Development of quantitative detection method for virulent strain of Vibrio parahaemolyticus and application for seafood in field

• Project/Area Number: 21790552
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hygiene
• Research Institution: Kyoto University
• Principal Investigator: NAKAGUCHI Yoshitsugu Kyoto University, 東南アジア研究所, 助教 (70378967)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 腸炎ビブリオ / 腸管感染症 / 下痢症 / 東南アジア / 魚介類 / 食品衛生 / 魚介類汚染 / 感染症 / 転写産物 / タイ

Research Abstract

Southeast Asian countries supply seafood to many countries. To achieve safety of seafood, the system of quantitative detection method is needed for quantitative detection of virulent strains of Vibrio parahaemolyticus. TRC method was applied for risk assessment of seafood contaminated by virulent strains of Vibrio parahaemolyticus. This system was practiced at the field of Thailand.

Research Products

• [Journal Article] Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freashwater hypermarket level (2011)
  • Author(s): Noorlis,A., Ghazali,F.M.,Cheah, Y.K., Tuan Zainazor, T.C., Ponniah,J., Tunung,R., Tang,J.Y.H., Nishibuchi,M., Nakaguchi,Y.Son,R.
  • Journal Title: International Food Research Journal 18 Pages: 673-679
  • Peer Reviewed
• [Journal Article] Prevalence and quantification of Vibrio species and Vibrio parahaemolyticus in freashwater hypermarket level (2011)
  • Author(s): Noorlis, A., Ghazali, F.M., Cheah, Y.K., Tuan Zainazor, T.C., Ponniah, J., Tunung, R., Tang, J.Y.H., Nishibuchi, M., Nakaguchi, Y., Son, R.
  • Journal Title: International Food Research Journal Volume: 18 Pages: 673-679
  • Peer Reviewed
• [Journal Article] I nvestigation of stx_2^+ eae^+ Escherichia coli O1 57 : H7 in beef imported from Malyasia to Thailand (2010)
  • Author(s): Sukhumungoon,P., Nakaguchi,Y., Ingviya,N., Pradutkanchana,J., Iwade,Y., Seto,K.Son,R., Nishibuchi,M.Vuddhakul,V.
  • Journal Title: International Food Research Journal. 18 Pages: 381-386
  • Peer Reviewed
• [Journal Article] Prevalence and Quantification of Vibrio parahaemolyticus in Raw Salad Vegetables at Retail Level (2010)
  • Author(s): Tunung,R., S.P.Margaret,P.Jeyaletchumi,L.C.Chai, T.C.Tuan Zainazor, F.M.Ghazali,Y.Nakaguchi,M.Nishibuchi, R.Son.
  • Journal Title: Journal of Microbiological Biotechnology. 20 Pages: 391-396
  • Peer Reviewed
• [Journal Article] Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes in Vibrio parahaemolyticus and related Vibrio species. (2010)
  • Author(s): Yamazaki,W., Kumeda,Y., Misawa,N., Nakaguchi,Y., Nishibuchi,M.
  • Journal Title: Applied Environmental Microbiology. 76(3) Pages: 820-828
  • Peer Reviewed
• [Journal Article] Prevalence and Quantification of Vibrio parahaemolyticus in Raw Salad Vegetables at Retail Level (2010)
  • Author(s): Tunung, R., S.P.Margaret, P.Jeyaletchumi, L.C.Chai, T.C.Tuan Zainazor, F.M.Ghazali, Y.Nakaguchi, M.Nishibuchi, R.Son.
  • Journal Title: Journal of Microbiological Biotechnology Volume: 20 Pages: 391-396
  • Peer Reviewed
• [Journal Article] Investigation of stx_2^+ eae^+ Escherichia coli O157:H7 in beef imported from Maly asia to Thailand (2010)
  • Author(s): Sukhumungoon, P., Nakaguchi, Y., Ingviya, N., Pradutkanchana, J., Iwade, Y., Seto, K., Son, R., Nishibuchi, M., Vuddhakul, V.
  • Journal Title: International Food Research Journal Volume: 18 Pages: 381-386
  • Peer Reviewed
• [Journal Article] Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes in Vibrio parahaemolyticus and related Vibrio species (2010)
  • Author(s): Wataru Yamazaki, Yoshitsugu Nakaguchi, et al.
  • Journal Title: Applied Environmental Microbiology 76(3) Pages: 820-828
  • Peer Reviewed
• [Presentation] ベトナム北部ハノイ市における腸炎ビブリオ感染症調査 (2010)
  • Author(s): 中口義次、Nguyen Binh Minh、Cuong Ngo Tuan、Tran Hoang Huy、Nguyen Hoai T hu、Le Thanh Huong、勢戸和子、大久保和洋、岩出義人、西渕光昭
  • Organizer: 第44回腸炎ビブリオシンポジウム
  • Place of Presentation: 秋田県秋田市
• [Presentation] 腸炎ビブリオのK抗原をコードするDNA領域の比較解析 (2010)
  • Author(s): 瀬尾晃司、権平文夫、勢戸和子、山崎渉、岩出義人、杉山純一、中口義次、西渕光昭
  • Organizer: 第44回腸炎ビブリオシンポジウム
  • Place of Presentation: 秋田県秋田市
• [Presentation] ベトナム・ハノイにおける腸炎ビブリオ感染症に関する調査 (2010)
  • Author(s): 中口義次、Nguyen Binh Minh、Cuong Ngo Tuan、Tran Hoang Huy、Nguyen Hoai T hu、Le Thanh Huong、勢戸和子、大久保和洋、岩出義人、西渕光昭
  • Organizer: 第51回日本熱帯医学会大会
  • Place of Presentation: 宮城県仙台市
• [Presentation] O:K serotype of Vibrio parahaemolyticus : a very important epidemiological marker (2010)
  • Author(s): Koji Seo, Fumio Gondaira, Junichi Sug iyama, Pharanai Sukhumungoon, Varaporn Vuddhakul, Wataru Yamazaki, Kazuko Seto, Yo shito Iwade, Rika Shimizu, Natsuko Tanaka, Yoshitsugu Nakaguchi, Mitsuaki Nishibuchi
  • Organizer: 45th A nnual Joint Panel Meeting on Cholera & Other Bacterial Enteric Infections Panel Meeting, United States-Japan Cooperative Medical Sci ence Program.
  • Place of Presentation: Kyoto (Japan)
• [Presentation] The First Reported Vibrio parahaemolyticus Diarrheal Case from Batam, Indonesiaand Some additional Related Epidemiological Characteristics 45th Annual Joint Panel Meeting on Cholera & Other Bacterial Enteric Infections Panel Meeting (2010)
  • Author(s): Abdul Aziz Djamal, Harry Fajri Zisoni, Yoshitsugu Nakaguchi, Kazuko Seto, Mitsuaki Nishibuchi
  • Organizer: United States-Japan Cooperative Medical Science Program.
  • Place of Presentation: Kyoto(Japan).
• [Presentation] Surveillance of Vibrio parahaemolyticus Infection in Hanoi, Vietnam 45th Annual Joint Panel Meeting on Cholera & Other Bacterial Enteric Infections Panel Meeting (2010)
  • Author(s): Yoshitsugu Nakaguchi, Nguyen Binh Minh, Cuong Ngo Tuan, Tran Hoang Huy, Nguyen Hoai Thu, Le Thanh Huong, Kazuko Seto, Kazuhiro Okubo, Yoshito Iwade, Mitsuaki Nishibuchi
  • Organizer: United States-Japan Cooperative Medical Science Program.
  • Place of Presentation: Kyoto(Japan).
• [Presentation] ベトナム北部ハノイ市における腸炎ビブリオ感染症調査 (2010)
  • Author(s): 中口義次、Nguyen Binh Minh、Cuong Ngo Tuan、Tran Hoang Huy、Nguyen Hoai Thu、Le Thanh Huong、勢戸和子、大久保和洋、岩出義人、西渕光昭
  • Organizer: 第44回腸炎ビブリオシンポジウム
  • Place of Presentation: 秋田県秋田市(秋田ビューホテル)
• [Presentation] 腸炎ビブリオのK抗原をコードするDNA領域の比較解析 (2010)
  • Author(s): 瀬尾晃司、権平文夫、勢戸和子、山崎渉、岩出義人、杉山純一、中口義次、西渕光昭
  • Organizer: 第44回腸炎ビブリオシンポジウム
  • Place of Presentation: 秋田県秋田市(秋田ビューホテル)
• [Presentation] ベトナム・ハノイにおける腸炎ビブリオ感染症に関する調査 (2010)
  • Author(s): 中口義次、Nguyen Binh Minh、Cuong Ngo Tuan、Tran Hoang Huy、Nguyen Hoai Thu、Le Thanh Huong、勢戸和子、大久保和洋、岩出義人、西渕光昭
  • Organizer: 第51回日本熱帯医学会大会
  • Place of Presentation: 宮城県仙台市(仙台国際センター)
• [Presentation] O : K serotype of Vibrio parahaemolyticus : a very important epidemiological marker (2010)
  • Author(s): Koji Seo, Fumio Gondaira, Junichi Sugiyama, Pharanai Sukhumungoon, Varaporn Vuddhakul, Wataru Yamazaki, Kazuko Seto, Yoshito Iwade, Rika Shimizu, Natsuko Tanaka, Yoshitsugu Nakaguchi, Mitsuaki Nishibuchi
  • Organizer: 45th Annual Joint Panel Meeting on Cholera & Other Bacterial Enteric Infections Panel Meeting, United States-Japan Cooperative Medical Science Program
  • Place of Presentation: Kyoto, Japan(稲盛財団記念会館)
• [Presentation] The First Reported Vibrio parahaemolyticus Diarrheal Case from Batam, Indonesia and Some additional Related Epidemiological Characteristics (2010)
  • Author(s): Abdul Aziz Djamal, Harry Fajri Zisoni, Yoshitsugu Nakaguchi, Kazuko Seto, Mitsuaki Nishibuchi
  • Organizer: 45th Annual Joint Panel Meeting on Cholera & Other Bacterial Enteric Infections Panel Meeting, United States-Japan Cooperative Medical Science Program
  • Place of Presentation: Kyoto, Japan(稲盛財団記念会館)
• [Presentation] Surveillance of Vibrio parahaemolyticus Infection in Hanoi, Vietnam (2010)
  • Author(s): Yoshitsugu Nakaguchi, Nguyen Binh Minh, Cuong Ngo Tuan, Tran Hoang Huy, Nguyen Hoai Thu, Le Thanh Huong, Kazuko Seto, Kazuhiro Okubo, Yoshito Iwade, Mitsuaki Nishibuchi
  • Organizer: 45th Annual Joint Panel Meeting on Cholera & Other Bacterial Enteric Infections Panel Meeting, United States-Japan Cooperative Medical Science Program
  • Place of Presentation: Kyoto, Japan(稲盛財団記念会館)
• [Presentation] LAMP法による腸炎ビブリオtdh,trh1,trh2の簡易迅速検出法の開発 (2009)
  • Author(s): 山崎渉、久米田裕子、中口義次、西渕光昭
  • Organizer: 第43回腸炎ビブリオシンポジウム
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2009-11-27
• [Presentation] LAMP法による腸炎ビブリオtdh, trh1, trh2の簡易迅速検出法の開発 (2009)
  • Author(s): 山崎渉、久米田裕子、中口義次、西渕光昭
  • Organizer: 第43回腸炎ビブリオシンポジウム
  • Place of Presentation: 岡山市
  • Year and Date: 2009-11-27
• [Presentation] Molecular biology study on gene expression, transcription and mRNA of virulent genes in Vibrio parahaemolyticus (2009)
  • Author(s): Yoshitsugu Nakaguchi, (Keynote speech)
  • Organizer: The 2nd International Seminar and Workshop on Advance Molecular Biology.
  • Place of Presentation: Padang (Indonesia).
  • Year and Date: 2009-08-19
• [Presentation] Antibiotic Resistance Pattern, Plasmid Profiling, and Random Amplified Polymorphic DNA (RAPD) Analysis of Vibrio vulnificus Isolated from Seawater in Padang, West Sumatera (2009)
  • Author(s): Yuherman, Yoshitsugu Nakaguchi, et al.
  • Organizer: The 2nd International Seminar and Workshop on Advance Molecular Biology.
  • Place of Presentation: Padang (Indonesia).
  • Year and Date: 2009-08-19
• [Presentation] Molecular biology study on gene expression, transcription and mRNA of virulent genes in Vibrio parahaemolyticus (Keynote speech) (2009)
  • Author(s): Yoshitsugu NAKAGUCHI
  • Organizer: The 2nd International Seminar and Workshop on Advance Molecular Biology
  • Place of Presentation: パダン(インドネシア)
  • Year and Date: 2009-08-19
• [Presentation] Antibiotic Resistance Pattern, Plasmid Profiling, and Random Amplified Polymorphic DNA (RAPD) Analysis of Vibrio vulnificus Isolated from Seawater in Padang, West Sumatera (2009)
  • Author(s): Yuherman, Yoshitsugu Nakaguchi, et al.
  • Organizer: The 2nd International Seminar and Workshop on Advance Molecular Biology
  • Place of Presentation: パダン(インドネシア)
  • Year and Date: 2009-08-19
• [Book] 食品により媒介される感染症等に関する文調査報告書 (2011)
  • Author(s): 中口義次, 他20名
  • Total Pages: 376
  • Publisher: 社団法人畜産技術協会
• [Book] 食品により媒介される感染症等に関する文献調査報告書 (2010)
  • Author(s): 中口義次, 他20名
  • Total Pages: 376
  • Publisher: 社団法人 畜産技術協会
• [Remarks] ホームページ等