| Project/Area Number |
21790601
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Public health/Health science
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
YOSHIMITSU Masato Osaka Prefectural Institute of Public Health, 衛生化学部, 研究員 (70321940)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | 亜硫酸 / DNA抽出 / 塩基配列 / シークエンス / DNA / 食品 |
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| Research Abstract |
In this study, I have tried to develop the method for analyzing DNA sequence of dried fruits, which were treated with sulfites as a preservative.
As a result, in specific dried fruits, it was suggested that some nucleotides were converted to another, and it was not possible to analyze the DNA sequence, although the amount of PCR amplification products was enough to use. Using real time PCR system with SYBR green fluorescent reagent, it was possible to detect dried fruits more clearly than using thermal cycler and electrophoresis, and the real time PCR products were useful for direct sequencing of nucleotides.
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