Innovative Therapy for Diabetes : ・-cell Replacement and Preservation

• Project/Area Number: 21790890
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: FUJIKURA Junji Kyoto University, 医学研究科, 助教 (70378743)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 糖尿病 / レプチン / 栄養学 / 移植・再生医療 / 再生医学 / 発生・分化

Research Abstract

We crossmated leptin-overexpressing transgenic mice with Akita mice and examined therapeutic effects of leptin on insulin-deficient diabetes mellitus. Leptin has a therapeutic impact on the onset and progression of glucose intolerance, diabetic complications and longevity in a mouse model of insulin-deficient nonobese diabetes. These data suggest that leptin may be useful as a long-term therapeutic agent for treating human diabetes mellitus.

Research Products

• [Journal Article] インスリン分泌低下型糖尿病を来すAkitaマウスにおけるレプチンの治療効果(京都大学大学院医学研究科内分泌代謝内科) (2011)
  • Author(s): 藤倉純二
  • Journal Title: 日本応用酵素協会誌 (0913-3348)45号 Pages: 84-85
• [Journal Article] 【メタボリックシンドローム(第2版) 基礎・臨床の最新知見】検査・診断 メタボリックシンドロームの病態診断 インスリン抵抗性の評価法HOMA-R法(京都大学医学部附属病院内分泌代謝内科) (2011)
  • Author(s): 藤倉純二, 内藤雅喜, 中尾一和
  • Journal Title: 日本臨床(メタボリックシンドローム) (0047-1852)69巻増刊1 Pages: 483-486
• [Journal Article] Podocyte-specific expression of tamoxifen-inducible Cre recombinase in mice. (2010)
  • Author(s): Yokoi H, Kasahara M, Mukoyama M, Mori K, Kuwahara K, Fujikura J, Arai Y, Saito Y, Ogawa Y, Kuwabara T, Sugawara A, Nakao K.
  • Journal Title: Nephrol Dial Transplant.,PubMed PMID : 20150167. 25(7) Pages: 2120-2124
• [Journal Article] Adipose tissue-specific regulation of angiotensinogen in obese humans and mice : impact of nutritional status and adipocyte hypertrophy. (2010)
  • Author(s): Yasue S, Masuzaki H, Okada S, Ishii T, Kozuka C, Tanaka T, Fujikura J, Ebihara K, Hosoda K, Katsurada A, Ohashi N, Urushihara M, Kobori H, Morimoto N, Kawazoe T, Naitoh M, Okada M, Sakaue H, Suzuki S, Nakao K.
  • Journal Title: Am J Hypertens.,PubMed PMID : 20057360 ; PubMed Central PMCID : PMC2842463. 23(4) Pages: 425-431
• [Journal Article] Adipose tissue-specific dysregulation of angiotensinogen by oxidative stress in obesity. (2010)
  • Author(s): Okada S, Kozuka C, Masuzaki H, Yasue S, Ishii-Yonemoto T, Tanaka T, Yamamoto Y, Noguchi M, Kusakabe T, Tomita T, Fujikura J, Ebihara K, Hosoda K, Sakaue H, Kobori H, Ham M, Lee YS, Kim JB, Saito Y, Nakao K.
  • Journal Title: Metabolism.,PubMed PMID : 20045538 ; PubMed Central PMCID : PMC2891233. 59(9) Pages: 1241-1251
• [Journal Article] Glucocorticoid reamplification within cells intensifies NF-kappaB and MAPK signaling and reinforces inflammation in activated preadipocytes. (2010)
  • Author(s): Ishii-Yonemoto T, Masuzaki H, Yasue S, Okada S, Kozuka C, Tanaka T, Noguchi M, Tomita T, Fujikura J, Yamamoto Y, Ebihara K, Hosoda K, Nakao K.
  • Journal Title: Am J Physiol Endocrinol Metab. Pub Med PMID : 19776225. 298(5),299(1) Pages: 930-940
• [Journal Article] 肥満症(第2版)基礎・臨床研究の進歩肥満症の疫学・病態・診断学の進歩症候性肥満病因、病態、管理法内分泌性肥満インスリノーマ(京都大学医学部附属病院内分泌代謝内科) (2010)
  • Author(s): 藤倉純二, 内藤雅喜, 細田公則, 中尾一和
  • Journal Title: 日本臨床(肥満症) (0047-1852)68巻増刊号2 Pages: 476-480
• [Journal Article] Adipose tissue-specific dysregulation of angiotensinogen by oxidative stress in obesity. (2010)
  • Author(s): Okada S, Kozuka C, Masuzaki H, Yasue S, Ishii-Yonemoto T, Tanaka T, Yamamoto Y, Noguchi M, Kusakabe T, Tomita T, Fujikura J, Ebihara K, Hosoda K, Sakaue H, Kobori H, Ham M, Lee YS, Kim JB, Saito Y, Nakao K.
  • Journal Title: Metabolism. Volume: 59 Pages: 1241-51
  • Peer Reviewed
• [Journal Article] Podocyte-specific expression of tamoxifen-inducible Cre recombinase in mice. (2010)
  • Author(s): Yokoi H, Kasahara M, Mukoyama M, Mori K, Kuwahara K, Fujikura J, Arai Y, Saito Y, Ogawa Y, Kuwabara T, Sugawara A, Nakao K.
  • Journal Title: Nephrology Dialysis Transplantation. Volume: 25 Pages: 2120-4
  • Peer Reviewed
• [Journal Article] Glucocorticoid reamplification within cells intensifies NF{kappa}B and MAPK signaling and reinforces inflammation in activated preadipocytes. (2010)
  • Author(s): Ishii-Yonemoto T, Masuzaki H, Yasue S, Okada S, Kozuka C, Tanaka T, Noguchi M, Tomita T, Fujikura J, Yamamoto Y, Ebihara K, Hosoda K, Nakao K.
  • Journal Title: American Journal of Physiology Endocrinology and Metabolism. Volume: 298
  • Peer Reviewed
• [Journal Article] Podocyte-specific expression of tamoxifen-inducible Cre recombinase in mice. (2010)
  • Author(s): Yokoi H, Kasahara M, Mukoyama M, Mori K, Kuwahara K, Fujikura J, Arai Y, Saito Y, Ogawa Y, Kuwabara T, Sugawara A, Nakao K.
  • Journal Title: Nephrology Dialysis Transplantation. (in press)
  • Peer Reviewed
• [Journal Article] Adipose tissue-specific regulation of angiotensinogen in obese humans and mice : impact of nutritional status and adipocyte hypertrophy. (2010)
  • Author(s): Yasue S, Masuzaki H, Okada S, Ishii T, Kozuka C, Tanaka T, Fujikura J, Ebihara K, Hosoda K, Katsurada A, Ohashi N, Urushihara M, Kobori H, Morimoto N, Kawazoe T, Naitoh M, Okada M, Sakaue H, Suzuki S, Nakao K.
  • Journal Title: American Journal of Hypertension. 4 Pages: 425-431
  • Peer Reviewed
• [Journal Article] Adipose tissue-specific dysregulation of angiotensinogen by oxidative stress in obesity. (2010)
  • Author(s): Okada S, Kozuka C, Masuzaki H, Yasue S, Ishii-Yonemoto T, Tanaka T, Yamamoto Y, Noguchi M, Kusakabe T, Tomita T, Fujikura J, Ebihara K, Hosoda K, Sakaue H, Kobori H, Ham M, Lee YS, Kim JB, Saito Y, Nakao K.
  • Journal Title: Metabolism. (in press)
  • Peer Reviewed
• [Journal Article] Glucocorticoid reamplification within cells intensifies NF{kappa}B and MAPK signaling and reinforces inflammation in activated preadipocytes. (2010)
  • Author(s): Ishii-Yonemoto T, Masuzaki H, Yasue S, Okada S, Kozuka C, Tanaka T, Noguchi M, Tomita T, Fujikura J, Yamamoto Y, Ebihara K, Hosoda K, Nakao K.
  • Journal Title: American Journal of Physiology Endocrinology and Metabolism. (in press)
  • Peer Reviewed
• [Presentation] Leptin Transgenic Akita mouseを用いたインスリン分泌低下型糖尿病に対するレプチン治療 (2010)
  • Author(s): 内藤雅喜, 藤倉純二, 海老原健, 宮永史子, 日下部徹, 宮本理人, 山本祐二, 野口倫生, 冨田努, 孫徹, 細田公則, 中尾一和
  • Organizer: 第31回日本肥満学会
  • Place of Presentation: 前橋テルサ
  • Year and Date: 2010-10-01
• [Presentation] インスリン分泌低下型糖尿病を来すAkitaマウスにおけるレプチンの治療効果の検討 (2010)
  • Author(s): 内藤雅喜, 藤倉純二, 野口倫生, 冨田努, 森栄作, 小鳥真司, 日下部徹, 山本祐二, 宮本理人, 海老原健, 益崎裕章, 細田公則, 中尾一和
  • Organizer: 第53回日本糖尿病学会
  • Place of Presentation: 岡山市デジタルミュージアム
  • Year and Date: 2010-05-29
• [Presentation] Physiological roles of the Hes1 in the pancreas. (2010)
  • Author(s): Junji Fujikura, Masaki Naito, Kiminori Hosoda, Hiroshi Iwakura, Tsutomu Tomita, Michio Noguchi, Shinji Odori, Eisaku Mori, Chisako Kozuka, Yuji Yamamoto, Ken Ebihara, Hiroaki Masuzaki, Yoshiya Kawaguchi, Rhoichiro Kageyama, Kazuwa Nakao
  • Organizer: 14^<th> International Congress of Endocrinology
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2010-03-28
• [Presentation] Physiological roles of the Hes1 in the pancreas. (2010)
  • Author(s): Fujikura, J, Naito, M, Hosoda, K, Iwakura, H, Tomita, T, Noguchi, M, Odori, S, Mori, E, Kozuka, C, Yamamoto, Y, Ebihara, K, Masuzaki, H, Kawaguchi, Y, Kageyama, R, Nakao, K
  • Organizer: 14th International Congress of Endocrinology
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-03-28
• [Presentation] Leptin ameliorates hyperglycemia in diabetic, insulin-deficient non-obese Akita mice (2010)
  • Author(s): Masaki Naito, Junji Fujikura, Kiminori Hosoda, Hiroshi Iwakura, Tsutomu Tomita, Michio Noguchi, Shinji Odori, Eisaku Mori, Toru Kusakabe, Yuji Yamamoto, Ken Ebihara, Hiroaki Masuzaki, Kazuwa Nakao
  • Organizer: 14^<th> International Congress of Endocrinology
  • Place of Presentation: Kyoto International Conference Center
  • Year and Date: 2010-03-27
• [Presentation] Leptin ameliorates hyperglycemia in diabetic, insulin-deficient non-obese Akita mice. (2010)
  • Author(s): Naito, M, Fujikura, J, Hosoda, K, Iwakura, H, Tomita, T, Noguchi, M, Odori, S, Mori, E, Kusakabe, T, Yamamoto, Y, Ebihara, K, Masuzaki, H, Nakao, K
  • Organizer: 14th International Congress of Endocrinology
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-03-27
• [Presentation] Notch/Rbp-jシグナルと膵転写因子との相互作用 (2009)
  • Author(s): 藤倉純二, 細田公則, 内藤雅喜, 森栄作, 小鳥真司, 野口倫生, 冨田努, 岩倉浩, 小塚智沙代, 岡田定規, 泰江慎太郎, 石井崇子, 宮本理人, 田中智洋, 日下部徹, 山本祐二, 平田雅一, 海老原健, 益崎裕章, 中尾一和
  • Organizer: 第52回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-05-22
• [Presentation] Notch/Rbp-jシグナルと膵転写因子との相互作用 (2009)
  • Author(s): 藤倉純二, 細田公則, 内藤雅喜, 森栄作, 小鳥真司, 野口倫生, 冨田努, 岩倉浩, 小塚智沙代, 岡田定規, 泰江慎太郎, 石井崇子, 宮本理人, 田中智洋, 日下部徹, 山本祐二, 平田雅一, 海老原健, 益崎裕章, 中尾一和
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 群馬県民会館
  • Year and Date: 2009-04-23
• [Remarks] ホームページ等
  • URL: http://www.kuhp.kyoto-u.ac.jp/~med2/jpn/research/diabetes.html
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~med2/jpn/research/diabetes.html
• [Remarks]
  • URL: http://www.kuhp.kyoto-u.ac.jp/~med2/jpn/research/diabetes.html