Functional analysis of ZNF521 in hematopoietic cell differentiation

• Project/Area Number: 21790919
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Ehime University
• Principal Investigator: YAMANOUCHI Jun Ehime University, 医学部附属病院, 講師 (10423451)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ZNF521 / Evi3 / 赤血球

Research Abstract

ZNF521(zinc finger protein 521) is a transcription factor with an N-terminal transcriptional repressor motif and 30 zinc finger domains. Although a high expression level of ZNF521 in human CD34+ progenitors and hematopoietic malignancies has been demonstrated, the functional role of ZNF521 in hematopoietic cell differentiation has not been clarified. In this study, we analyzed the role of ZNF521 in erythroid cell differentiation using the short hairpin RNA (shRNA)-mediated gene silencing method.

Research Products

• [Journal Article] Vasodilator-stimulated phosphoprotein (VASP) phosphorylation assay for platelet response to cilostazol. (2011)
  • Author(s): Yamanouchi J, Hato T, Niiya T, Nakagawa K, Kumon Y, Fujiwara H, Yakushijin Y, Yasukawa M.
  • Journal Title: Platelets 22 Pages: 135-142
  • Peer Reviewed
• [Journal Article] Vasodilator-stimulated phosphoprotein (VASP) phosphorylation assay for platelet response to cilostazol (2011)
  • Author(s): Yamanouchi J, Hato T, Niiya T, Nakagawa K, Kumon Y, Fujiwara H, Yakushijin Y, Yasukawa M
  • Journal Title: Platelets Volume: 22 Pages: 135-142
  • Peer Reviewed
• [Journal Article] Derivative (1;18)(q10;q10) in essential thrombocythemia. (2010)
  • Author(s): Azuma T, Yamanouhi J, Inoue K, Kohno M, Narumi H, Fujiwara H, Yakushijin Y, Hato T, Yasukawa M.
  • Journal Title: Cancer Genetics And Cytogenetics 199 Pages: 62-64
  • Peer Reviewed
• [Journal Article] Derivative (1;18)(g10;q10) in essential thrombocythemia (2010)
  • Author(s): Azuma T, Yamanouhi J, Inoue K, Kohno M, Narumi H, Fujiwara H, Yakushijin Y, Hato T, Yasukawa M
  • Journal Title: Cancer Genet Cytogenet. Volume: 199 Pages: 62-64
  • Peer Reviewed
• [Journal Article] The role of zinc finger protein 521/ early hematopoietic zinc finger protein in erythroidcell differentiation. (2009)
  • Author(s): Matsubara E, Sakai I, Yamanouchi J, Fujiwara H, Yakushijin Y, Hato T, Shigemoto K, Yasukawa M.
  • Journal Title: Journal of Biological Chemistry 284 Pages: 3480-3487
  • Peer Reviewed
• [Journal Article] Identification of a novel epitope derived from CML66 that is recognized by anti-leukemia cytotoxic T lyphocytes. (2009)
  • Author(s): Suemori K, Fujiwara H, Ochi T, Azuma T, Yamanouchi J, Narumi H, Yakushijin Y, Hato T, Yasukawa M.
  • Journal Title: British Journal of Haematology 146 Pages: 115-118
  • Peer Reviewed
• [Journal Article] Compound heterozygous mutations in the PROS1 gene responsible for quantitative and qualitative protein S deficiency. (2009)
  • Author(s): Yamanouchi J, Hato T, Tamura T, Fujiwara H, Yakushijin Y, Yasukawa M.
  • Journal Title: International Journal of Hematology 90 Pages: 537-539
  • Peer Reviewed
• [Journal Article] The role of zinc finger protein 521/early hematopoietic zinc finger protein in erythroid cell differentiation. (2009)
  • Author(s): Matsubara E, Sakai I, Yamanouchi J, et ai.
  • Journal Title: Journal of Biological Chemistry 284 Pages: 3480-3487
  • Peer Reviewed
• [Journal Article] Identification of a novel epitope derived from CML66 that is recognized by anti-leukemia cytotoxic T lyphocytes. (2009)
  • Author(s): Suemori K, Fujiwara H, Ochi T, Azuma T, Yamanouchi J, et al.
  • Journal Title: British Journal of Haematology 146 Pages: 115-118
  • Peer Reviewed
• [Journal Article] Compound heterozygous mutations in the PROS1 gene responsible for quantitative and qualitative protein S deficiency. (2009)
  • Author(s): Yamanouchi J., et al.
  • Journal Title: International Journal of Hematology 90 Pages: 537-539
  • Peer Reviewed
• [Presentation] The β tail domain (βTD) in the integrin β3 subunit regulates αIIbβ3 activation. (2010)
  • Author(s): Yamanouchi J, Hato T, Fujiwara H, Yakushijin Y, Yasukawa M.
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-09-24
• [Presentation] The β tail domain (βTD) in the integrin β3 subunit regulates αIIbβ3 activation (2010)
  • Author(s): 山之内純
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-09-24
• [Presentation] CD8陽性PTCL細胞のパーフォリン依存性細胞傷害には、腫瘍細胞上のCD3/T細胞受容体複合体が関与している (2009)
  • Author(s): 安軍、藤原弘、末盛浩一郎、越智俊元、永井功造、峰野純一、白方俊章、山之内純、薬師神芳洋、羽藤高明、安川正貴
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2009-10-25
• [Presentation] Tie2-Cre STAT3コンディショナルノックアウトマウスは溶血性貧血を呈する (2009)
  • Author(s): 山之内純、藤原弘、越智俊元、白方俊章、薬師神芳洋、羽藤高明、平川聡史、大久保信孝、鈴木洋司、満田憲昭、安川正貴
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2009-10-24
• [Presentation] Development of cancer gene-immunotherapy using Aurora-A kinase-specific T-cell receptor genes (2009)
  • Author(s): Fujiwara H, Ochi T, Nagai K, Shirakata T, Yamanouchi J, Mineno J, Kuzushima K, Yakushijin Y, Hato T, Yasukawa M.
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2009-10-23
• [Presentation] Integrin β3 Membrane-Proximal And -Distal Residues Cooperatively Regulate Talin-Mediated αIIbβ3 Acitivation. (2009)
  • Author(s): Yamanouchi J, Hato T, Fujiwara H, Yakushijin Y, Yasukawa M.
  • Organizer: 22th Congress of The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston, MA, U.S.A
  • Year and Date: 2009-07-15
• [Presentation] Integrin β3 Membrane-Proximal And-Distal Residues Cooperatively Regulate Talin-Mediated αIIbβ3 Acitivation (2009)
  • Author(s): Yamanouchi J., et al.
  • Organizer: 22th Congress of The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston, MA, U.S.A
  • Year and Date: 2009-07-15
• [Presentation] Compound Heterozygous Mutations In The PROS1 Gene Responsible For Quantitative And Qualitative Protein S Deficiency. (2009)
  • Author(s): Yamanouchi J, Hato T, Tamura T, Fujiwara H, Yakushijin Y, Yasukawa M.
  • Organizer: 22th Congress of The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston, MA, U.S.A
  • Year and Date: 2009-07-13
• [Presentation] Compound Heterozygous Mutations In The PROS1 Gene Responsible For Quantitative And Qualitative Protein S Deficiency (2009)
  • Author(s): Yamanouchi J., et al.
  • Organizer: 22th Congress of The International Society on Thrombosis and Haemostasis
  • Place of Presentation: Boston, MA, U.S.A
  • Year and Date: 2009-07-13
• [Presentation] Toll-like receptorsを介した血小板インテグリンallbb3の活性化 (2009)
  • Author(s): 山之内純、羽藤高明
  • Organizer: 第32回日本血栓止血学会学術集会会
  • Place of Presentation: 北九州市
  • Year and Date: 2009-06-05
• [Presentation] Toll-like receptorsを介した血小板インテグリンallbb3の活性化 (2009)
  • Author(s): 山之内純, 他
  • Organizer: 第32回日本血栓止血学会学術集会
  • Place of Presentation: 北九州市
  • Year and Date: 2009-06-05
• [Remarks] ホームページ等