Analysis of cytokine production regulatory mechanism in basophils by immunoglobulin-like receptor PIR-B.

• Project/Area Number: 21790945
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Tohoku Institute of Technology
• Principal Investigator: SAKAMOTO Yuzuru Tohoku Institute of Technology, 共通教育センター, 准教授 (30316434)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 好塩基球 / PIR-B / IL-3 / ITIM / Th2免疫応答

Research Abstract

To examine the potential involvement of PIR-B, the inhibitory type murine paired immunoglobulin-like receptor, in the regulation of Th2-type cytokine production in basophils, we used retroviral gene transfer system and analyzed PIR-B-deficient mice bone marrow-derived basophils. As a result, we found that PIR-B regulates IL-3-induced IL-4 production in murine basophils. Moreover, from the result of analysis that used various PIR-B mutants, PIR-B expressed on basophils negatively regulates IL-3-induced IL-4 production in an ITIM-independent manner, pointing to previously unrecognized regulatory mechanisms by PIR-B.

Research Products

• [Journal Article] Differential but competitive binding of Nogo and MHCI to PIR-B provides an inhibition of cells.
  • Author(s): Matsushita H, Endo S, Kobayashi E, Sakamoto Y, Kobayashi K, Kitaguchi K, Kuroki K, Soderhall A, Maenaka K, Nakamura A, Strittmatter SM, Takai T
  • Journal Title: J Biol Chem (in press)
  • Peer Reviewed
• [Presentation] 抑制型レセプターによる好塩基球のTh2免疫応答制御について (2010)
  • Author(s): 坂本譲
  • Organizer: JSEI-1^<st> Spring Seminar 2010
  • Place of Presentation: 宮城
• [Presentation] Regulation of cytotoxic T lymphocyte activity by CD8 and PIR-B. (2010)
  • Author(s): Endo S, Sakamoto Y, Kobayashi E, Nakamura A, Takai T
  • Organizer: The 5th International Symposium of Institute Network
  • Place of Presentation: Kanazawa
• [Presentation] Kinetic analysis of the association between PIR-B and its related molecules in B cells. (2010)
  • Author(s): Sakamoto Y, Watanabe Y, Nakamura A, Takai T
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe
• [Presentation] Paired immunoglobulin-like receptor (PIR)-B regulates IL-3-induced IL-4 production in a novel ITIM-independent manner in murine basophils. (2010)
  • Author(s): Oda A, Sakamoto Y, Koshi I, Takai T, Hida S, Taki S.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: Kobe
• [Presentation] Regulation of cytotoxic T lymphocyte activity by CD8 and PIR-B. (2010)
  • Author(s): Endo S, Sakamoto Y, Kobayashi E, Nakamura A, Takai T.
  • Organizer: The 5th International Symposium of Institute Network
  • Place of Presentation: KKRホテル金沢(石川)
• [Presentation] Kinetic analysis of the association between PIR-B and its related molecules in B cells. (2010)
  • Author(s): Sakamoto Y, Watanabe Y, Nakamura A, Takai T.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸国際会議場(兵庫)
• [Presentation] Paired immunoglobulin-like receptor (PIR)-B regulates IL-3-induced IL-4 production in a novel ITIM-independent manner in murine basophils. (2010)
  • Author(s): Oda A, Sakamoto Y, Koshi I, Takai T, Hida S, Taki S.
  • Organizer: 14th International Congress of Immunology
  • Place of Presentation: 神戸国際会議場(兵庫)
• [Presentation] Competitive binding of MHC class I between PIR-B and CD8 regulates CTLactivity. (2009)
  • Author(s): Endo S, Sakamoto Y, Nakamura A, Takai T.
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2009-12-03
• [Presentation] Paired immunoglobulin-like receptor (PIR)-B attenuates IL-3-induced IL-4 production in a novel ITIM-independent manner in murine basophils. (2009)
  • Author(s): Sakamoto Y, Oda A, Takai T, Hida S, Taki S.
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 大阪国際会議場(大阪)
  • Year and Date: 2009-12-02
• [Presentation] Fc receptor γ-chain is a subunit of the IL-3 receptor, required for IL-4 production by murine basophils. (2009)
  • Author(s): Hida S, Sakamoto Y, Yamasaki S, Saito T, Taki S.
  • Organizer: 9th World Congress on Inflammation
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2009-07-07
• [Presentation] Fc receptor γ-chain is a subunit of the IL-3 receptor, required for IL-4 production by murine basophils. (2009)
  • Author(s): Hida S, Sakamoto Y, Yamasaki S, Saito T, Taki S
  • Organizer: 9th World Congress on Inflammation
  • Place of Presentation: Tokyo
• [Presentation] Competitive binding of MHC class I between PIR-B and CD8 regulates CTL activity. (2009)
  • Author(s): Endo S, Sakamoto Y, Nakamura A, Takai T
  • Organizer: 第39回日本免疫学会総会・学術集会記録第39巻, 128, 2009
  • Place of Presentation: 大阪
• [Presentation] Paired immunoglobulin-like receptor (PIR)-B attenuates IL-3-induced IL-4 production in a novel ITIM-independent manner in murine basophils. (2009)
  • Author(s): Sakamoto Y, Oda A, Hida S, Taki S
  • Organizer: 第39回日本免疫学会総会・学術集会記録第39巻, 95, 2009
  • Place of Presentation: 大阪