Elucidation of mechanisms of peroxisomal disorders using pluripotent stem cells
| Project/Area Number |
21790977
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pediatrics
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| Research Institution | Gifu University |
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Principal Investigator |
NAGASE Tomoko 岐阜大学, 生命科学総合研究支援センター, 助教 (20397326)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 小児神経学 / 脳神経疾患 / 発生・分化 / 再生医学 |
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| Research Abstract |
To reveal the involvements of peroxisome during early developmental stage and obtain some clues as to the underlying cause of peroxisome biogenesis disorders(PBD_s), we performed neural differentiation studies using mouse embryonic stem(mES) cells and human induced pluripotent stem(iPS) cells. Few punctate staining patterns of catalase were observed in neural precursor cells and oligodendrocyte precursor cells differentiated from mES cells. Furthermore, we established iPS cells from fibroblasts derived from PBD patients, and performed neural differentiation studies using SDIA method. There is no obvious difference in efficiency of neural differentiation between a normal healthy individual and PBD patients.
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Report
(4 results)
Research Products
(4 results)
