Rapid diagnosis for tiny sentinel node metastasis in malignant melanoma using RT-LAMP method
| Project/Area Number |
21791056
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Hirosaki University |
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Principal Investigator |
KANEKO Takahide Hirosaki University, 医学部附属病院, 講師 (20333718)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 悪性黒色腫 / RT-LAMP法 / センチネルリンパ節 / センチネルリンパ節生検 / RT-PCR |
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| Research Abstract |
Malignant melanoma is generally resistant for chemotherapy and radiation therapy, and one of malignant tumors with the worst prognosis. The purpose of this study is to establish rapid diagnosis for tiny sentinel node metastasis in malignant melanoma. As a result, we have performed sentinel node biopsies about approximate 30 cases of melanoma patients, resulting in total of 50 cases. In 82% of cases, RT-PCR diagnosis is correspond to pathological diagnosis while RT-PCR is different from pathological diagnosis in 18%. This result further confirms that RT-PCR diagnosis for tiny sentinel node metastasis in malignant melanoma is very useful method. In addition, we examined feasibility of RT-LAMP method and showed that RT-LAMP and RT-PCR methods have almost the same ability to detect tiny metastasis of malignant melanoma. We think we need further experiments to improve conditions of RT-LAMP method.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Relation between the expression levels of the POU transcription factors Skn-1a and Skn-1n and keratinocyte differentiation.2010
Author(s)
Takemoto H, Tamai K, Akasaka E, Rokunohe D, Takiyoshi N, Umegaki N, Nakajima K, Aizu T, Kaneko T, Nakano H, Sawamura D.
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Journal Title
J Dermatol Sci 60
Pages: 203-205
NAID
Related Report
Peer Reviewed
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[Journal Article] Lymphoscintigraphy for sentinel lymph node mapping in patients with malignant skin neoplasms of the lower extremrties.2010
Author(s)
Miura H, Ono S, Nagahata M, Shibutani K, Kakehata S, Morimoto K, Seino H, Kakuta A, Aoki M, Hatayama Y, Kawaguchi H, Takai Y, Abe Y, Kaneko T, Sawamura D, Hanada K.
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Journal Title
Ann Nucl Med 24
Pages: 601-608
Related Report
Peer Reviewed
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