Development of multi-functional radiosensitizer based on in vivo radiosensitizing activity using tumor-implant chick embryo

• Project/Area Number: 21791198
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: The University of Tokushima
• Principal Investigator: UTO Yoshihiro The University of Tokushima, 大学院・ソシオテクノサイエンス研究部, 准教授 (20304553)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 発育鶏卵 / 放射線増感剤 / 固形腫瘍 / 低酸素細胞放射線増感剤 / グルコース / 腫瘍移植鶏卵 / 薬物動態

Research Abstract

Chick embryo have been used as alternative experimental animals in various research fields, including virology, immunology, toxicology, oncology, and embryology. In this research, we developed the screening system of in vivo radiosensitizing activity of etanidazole as a radiosensitizer using tumor-bearing chick embryo. Moreover, we succeeded the evaluation of pharmacokinetic property of etanidazole using this model.

Research Products

• [Journal Article] Evaluation of the In vivo Radiosensitizing Activity of Etanidazole Using Tumor-bearing Chick Embryo (2011)
  • Author(s): Abe C, Uto Y, Nakae T, Shinmoto Y, Sano K, Nakata H, Teraoka M, Endo Y, Maezawa H, Masunaga S, Nakata E, Hori H
  • Journal Title: J Radiat Res 52 Pages: 208-214
  • NAID: 10027956760
  • Peer Reviewed
• [Journal Article] Synthesis and photophysical properties of new SNARF derivatives as dual emission pH sensors (2011)
  • Author(s): Nakata E, Nazumi Y, Yukimachi Y, Uto Y, Maezawa H, Hashimoto T, Okamoto Y, Hori H
  • Journal Title: Bioorg Med Chem Lett 21 Pages: 1663-1666
  • Peer Reviewed
• [Journal Article] Evaluation of the In vivo Radiosensitizing Activity of Etanidazole Using Tumor-bearing Chick Embryo. (2011)
  • Author(s): Abe C, Uto Y, et al.
  • Journal Title: Journal of Radiation Research Volume: 52 Pages: 208-214
  • NAID: 10027956760
  • Peer Reviewed
• [Journal Article] Medicinal electronomics bricolage design of hypoxia-targeting antineoplastic drugs and invention of boron tracedrugs as innovative future-architectural drugs (2010)
  • Author(s): Hori H, Uto Y, Nakata E
  • Journal Title: Anticancer Res 30 Pages: 3233-3242
  • Peer Reviewed
• [Journal Article] Vitamin D Binding Protein-Macrophage Activating Factor Inhibits HCC in SCID Mice (2010)
  • Author(s): Nonaka K, Onizuka S, Ishibashi H, Uto Y, Hori H, Nakayama T, Matsuura N, Kanematsu T, Fujioka H
  • Journal Title: J Surg Res in press
  • Peer Reviewed
• [Journal Article] A newly designed cell-permeable SNARF derivative as an effective intracellular pH indicator (2010)
  • Author(s): Nakata E, Yukimachi Y, Nazumi Y, Uto Y, Maezawa H, Hashimoto T, Okamoto Y, Hori H
  • Journal Title: Chem Commun (Camb) 46 Pages: 3526-3528
  • NAID: 110008621031
  • Peer Reviewed
• [Journal Article] Design of novel hypoxia-targeting IDO hybrid inhibitors conjugated with an unsubstituted L-TRP as an IDO affinity moiety (2010)
  • Author(s): Nakashima H, Ikkyu K, Nakashima K, Sano K, Uto Y, Nakata E, Nagasawa H, Sugimoto H, Shiro Y, Nakagawa Y, Hori H
  • Journal Title: Adv Exp Med Biol 662 Pages: 415-421
  • Peer Reviewed
• [Journal Article] Medicinal electronomics bricolage design of hypoxia-targeting antineoplastic drugs and invention of boron tracedrugs as innovative future-architectural drugs. (2010)
  • Author(s): Hori H, Uto Y, et al.
  • Journal Title: Anticancer Research Volume: 30 Pages: 3233-3242
  • Peer Reviewed
• [Journal Article] Design of a bioreductively-activated fluorescent pH probe for tumor hypoxia imaging (2009)
  • Author(s): Nakata E, Yukimachi Y, Kariyazono H, Im S, Abe C, Uto Y, Maezawa H, Hashimoto T, Okamoto Y, Hori H
  • Journal Title: Bioorg Med Chem 17 Pages: 6952-6958
  • NAID: 110007594198
  • Peer Reviewed
• [Journal Article] A chemical biosynthesis design for an antiatherosclerosis drug by acyclic tocopherol intermediate analogue based on "isoprenomics" (2009)
  • Author(s): Uto Y, Koyama D, Otsuki M, Otomo N, Shirai T, Abe C, Nakata E, Nagasawa H, Hori H
  • Journal Title: Adv Exp Med Biol 645 Pages: 109-114
  • Peer Reviewed
• [Journal Article] Downregulation of matrix metalloprotease-9 and urokinase plasminogen activator by TX-1877 results in decreased tumor growth and metastasis on xenograft model of rectal cancer (2009)
  • Author(s): Miyake K, Shimada M, Nishioka M, Sugimoto K, Batmunkh E, Uto Y, Nagasawa H, Hori H
  • Journal Title: Cancer Chemother Pharmacol 64 Pages: 885-892
  • Peer Reviewed
• [Journal Article] Correlation between antiangiogenic activity and antioxidant activity of various components from propolis (2009)
  • Author(s): Ahn MR, Kunimasa K, Kumazawa S, Nakayama T, Kaji K, Uto Y, Hori H, Nagasawa H, Ohta T
  • Journal Title: Mol Nutr Food Res 53 Pages: 643-651
  • Peer Reviewed
• [Journal Article] 次世代動物実験系としての腫瘍移植鶏卵の構築と放射線照射による腫瘍成長阻害活性 (2009)
  • Author(s): 安部千秋, 宇都義浩, 遠藤良夫, 新元優也, 中島宏一郎, 佐野圭一郎, 佐々木有紀, 皆巳和賢, 前澤博, 増永慎一郎, 中田栄司, 堀均
  • Journal Title: 放射線生物研究 44 Pages: 233-241
• [Journal Article] A chemical biosynthesis design for an antiatherosclerosis drug by acyclic tocopherol intermediate analogue based on "isoprenomics" (2009)
  • Author(s): Yoshihiro Uto
  • Journal Title: Advances in experimental medicine and biology 645 Pages: 109-114
  • Peer Reviewed
• [Journal Article] Downregulation of matrix metalloprotease-9 and urokinase plasminogen activator by TX-1877 results in decreased tumor growth and metastasis on xenograft model of rectal cancer (2009)
  • Author(s): Kotaro Miyake
  • Journal Title: Cancer chemotherapy and pharmacology 64 Pages: 885-892
  • Peer Reviewed
• [Journal Article] Artepillin C(ARC)in Brazilian green propolis selectively blocks oncoge-nic PAK1 signaling and suppresses the growth of NF tumors in mice (2009)
  • Author(s): Shanta M.Messerli
  • Journal Title: Phytotherapy research 23 Pages: 423-427
  • Peer Reviewed
• [Presentation] In vivo antioxidative activity of radical-containing-nanoparticle (RNP) in fertilized chicken egg assay (2011)
  • Author(s): Uto Y, Abe C, Yoshitomi T, Nagasaki Y, Endo Y, Nakata E, Hori H
  • Organizer: International Conference on Biomaterials Science 2011
  • Place of Presentation: つくば市
  • Year and Date: 2011-03-16
• [Presentation] 放射線増感活性における糖要求性からみたアセチル化グルコースハイブリッド放射線増感剤TX-2244のメディシナルケミストリー (2011)
  • Author(s): 宇都義浩, 中江崇, 金園剛行, 村井絵美, 佐野圭一郎, 寺岡瑞絵, 富永正英, 前澤博, 堀均
  • Organizer: 第13回癌治療増感研究シンポジウム
  • Place of Presentation: 奈良市
  • Year and Date: 2011-02-11
• [Presentation] Systems biology-based drug design of sugar-hybrid hypoxic cell radiosensitizers using the tumor-implantable chick embryo model (2010)
  • Author(s): Uto Y, Abe C, Nakae T, Endo Y, Tominaga M, Maezawa H, Nakata E, Hori H
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: ホノルル市
  • Year and Date: 2010-12-19
• [Presentation] Systems biology-based drug design of sugar-hybrid hypoxic cell radiosen sitizers using the tumor-implantable chick embryo model. (2010)
  • Author(s): Uto Y, et al.
  • Organizer: PACIFICHEM 2010
  • Place of Presentation: ハワイコンベンションセンタ(ホノルル)
  • Year and Date: 2010-12-19
• [Presentation] 癌免疫療法の確立を目指したマクロファージ活性化因子(GcMAF)前駆物質GcXの創製 (2010)
  • Author(s): 宇都義浩, 山本将太, 竹内亮太, 中川美典, 廣田慶司, 寺田弘, 鬼塚伸也, 中田栄司, 堀均
  • Organizer: 第14回バイオ治療法研究会学術集会
  • Place of Presentation: 福岡市
  • Year and Date: 2010-12-11
• [Presentation] SAR analysis of electron-rich polyyne analogues of FTY720 based on in vivo chick embryo antiangiogenic assay (2010)
  • Author(s): Uto Y, Abe C, Nakata E, Hori H
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-22
• [Presentation] Prenylated acylphloroglucinol derivatives : Isoprenomics-based design, syntheses and antioxidative activities (2010)
  • Author(s): Uto Y, Tanaka R, Ohnaka K, Ohta Y, Yazaki K, Umemoto N, Nakata E, Hori H
  • Organizer: International Society on Oxygen Transport to Tissue 2010
  • Place of Presentation: アスコナ(スイス)
  • Year and Date: 2010-07-19
• [Presentation] ストレス発育鶏卵モデルを用いたラジカル含有ナノ粒子TEMPO-RNPのin vivo抗酸化活性評価 (2010)
  • Author(s): 宇都義浩, 安部千秋, 吉冨徹, 長崎幸夫, 遠藤良夫, 中田栄司, 堀均
  • Organizer: 第63回日本酸化ストレス学会
  • Place of Presentation: 横浜市
  • Year and Date: 2010-06-24
• [Presentation] 腫瘍移植鶏卵モデルによる糖ハイブリッド放射線増感剤のin vivo放射線増感活性の評価 (2010)
  • Author(s): 宇都義浩, 安部千秋, 中江崇, 村井絵美, 遠藤良夫, 富永正英, 前澤博, 増永慎一郎, 中田栄司, 堀均
  • Organizer: 第16回国際癌治療増感研究会
  • Place of Presentation: 岐阜市
  • Year and Date: 2010-06-19
• [Presentation] 腫瘍移植鶏卵モデルによる糖ハイブリッド放射線増感剤のin vivo放射線増感活性の評価 (2010)
  • Author(s): 宇都義浩, 他
  • Organizer: 第16回国際癌治療増感研究会
  • Place of Presentation: 県民文化ホール未来会館(岐阜)
  • Year and Date: 2010-06-19
• [Presentation] 抗酸化剤と酸化ストレスの新しい評価法 (2010)
  • Author(s): 宇都義浩
  • Organizer: 第7回学際物質戦略イニシアチブ バイオGPワークショップ
  • Place of Presentation: つくば市
  • Year and Date: 2010-03-13
• [Presentation] システムバイオロジー構築のためのフィチルキノールの合成と抗酸化活性評価 (2010)
  • Author(s): 宇都義浩, 山下洋平, 田中涼, 中田栄司, 堀均
  • Organizer: 第21回ビタミンE研究会
  • Place of Presentation: 東京都
  • Year and Date: 2010-01-22
• [Presentation] 鶏胚異種移植腫瘍モデルを基盤とした糖ハイブリッド低酸素細胞放射線増感剤の創製 (2009)
  • Author(s): 中江崇, 宇都義浩, 安部千秋, 金園剛行, 新元優也, 佐野圭一郎, 中田栄司, 遠藤良夫, 富永正英, 前澤博, 橋本敏弘, 増永慎一郎, 堀均
  • Organizer: 創薬懇話会2009
  • Place of Presentation: 岐阜市
  • Year and Date: 2009-12-10
• [Presentation] Pharmacokinetic drug design of sugar-hybrid radiosensitizers using the tumor implant chick embryo model (2009)
  • Author(s): 宇都義浩
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-02
• [Presentation] 腫瘍移植鶏卵を用いて薬物動態を考慮した糖ハイブリッド放射線増感剤のドラッグデザイン (2009)
  • Author(s): 宇都義浩, 安部千秋, 遠藤良夫, 前澤博, 中田栄司, 堀均
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-01
• [Presentation] 腫瘍移植鶏卵モデルによる糖ハイブリッド放射線増感剤の薬物動態解析 (2009)
  • Author(s): 宇都義浩, 中江崇, 安部千秋, 新元優也, 佐野圭一郎, 遠藤良夫, 富永正英, 前澤博, 中田栄司, 堀均
  • Organizer: 第15回国際癌治療増感研究会
  • Place of Presentation: 京都市
  • Year and Date: 2009-06-20
• [Presentation] イソプレノミクスを基盤としたプレニル化フェニルプロパノイドの分子設計と発育鶏卵を用いたin ovo抗酸化活性の評価 (2009)
  • Author(s): 宇都義浩, 安部千秋, 山下洋平, 田中涼, 遠藤良夫, 中田栄司, 堀均
  • Organizer: 第62回日本酸化ストレス学会学術集会
  • Place of Presentation: 福岡市
  • Year and Date: 2009-06-11
• [Book] 酸素をターゲットとしたがんの治療 東京 (2009)
  • Author(s): 堀均, 宇都義浩, 永澤秀子, 中田栄司
  • Total Pages: 596
  • Publisher: 朝倉書店
• [Remarks] ホームページ等
  • URL: http://www.bio.tokushima-u.ac.jp/a2group/a2index.html