Elucidation of pathway and molecular mechanism of radiation-induced non-apoptotic cell death mode in human solid carcinoma cell

• Project/Area Number: 21791201
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Radiation science
• Research Institution: Nagasaki University
• Principal Investigator: SUZUKI Masatoshi Nagasaki University, 医歯薬学総合研究科, 研究員 (60515823)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 放射線 / 細胞死 / 老化様増殖停止 / 生細胞イメージング / マイトティック・カタストロフィ / ネクローシス

Research Abstract

Live-cell imaging of mammary carcinoma cell line revealed prolonged cell cycle arrest as a predominant cell death mode induced by ionizing radiation. Regardless cell cycle phase that exposed to ionizing radiation, prolonged cell cycle arrest was induced in G1 phase. Particularly, direct cell cycle progression from G2 to G1 phase without mitosis was observed by G2-irradiation in presence of p53, suggesting that radiochemotherapy combined with taxane-treatment increases the treatment-sensitivity for p53-depleted carcinoma cells.

Research Products

• [Journal Article] Creating localized DNA double-strand breaks with microirradiation. (2011)
  • Author(s): Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S
  • Journal Title: Nat.Protoc. 6(2) Pages: 134-139
  • NAID: 120006985036
  • Peer Reviewed
• [Journal Article] Creating localized DNA double-strand breaks with microirradiation (2011)
  • Author(s): Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S.
  • Journal Title: Nature Protocol Volume: Vol.6 Pages: 134-139
  • NAID: 120006985036
  • Peer Reviewed
• [Journal Article] Requirement of ATM-dependent pathway for the repair of a subset of DNA double strand breaks created by restriction endonucleases. (2010)
  • Author(s): Suzuki K, Takahashi M, Oka Y, Yamauchi M, Suzuki M, Yamashita S
  • Journal Title: Genome Integr. 1(1) Pages: 4-4
  • Peer Reviewed
• [Journal Article] A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks. (2010)
  • Author(s): Suzuki K, Yamauchi M, Oka Y, Suzuki M, Yamashita S
  • Journal Title: Nucl.Acids Res. 38(12)
  • NAID: 120006984199
  • Peer Reviewed
• [Journal Article] がん細胞における放射線誘発細胞死の系譜 (2010)
  • Author(s): 鈴木正敏、鈴木啓司、山下俊一
  • Journal Title: 広島医学 63(4) Pages: 334-336
• [Journal Article] 放射線による細胞致死の分子メカニズムとその生物学的意義 (2009)
  • Author(s): 鈴木啓司、鈴木正敏
  • Journal Title: 放射線生物研究 44 Pages: 11-18
  • NAID: 40016597007
• [Journal Article] 放射線による細胞致死の分子メカニズムとその生物学的意義 (2009)
  • Author(s): 鈴木啓司
  • Journal Title: 放射線生物研究 44 Pages: 11-18
  • NAID: 40016597007
• [Presentation] FUCCIシステム導入ヒト乳癌細胞を用いた生細胞ライブイメージングによる老化様増殖停止誘導過程における細胞周期解析 (2010)
  • Author(s): 鈴木正敏、鈴木啓司、山下俊一
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都
  • Year and Date: 2010-10-22
• [Presentation] FUCCIシステム導入ヒト乳癌細胞を用いた生細胞ライブイメージングによる老化様増殖停止誘導過程における細胞周期解析 (2010)
  • Author(s): 鈴木正敏
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都テルサ(京都府)
  • Year and Date: 2010-10-22
• [Presentation] Comprehensive pedigree analysis of cell death pathway in mammary carcinoma cell line exposed to ionizing radiation. (2010)
  • Author(s): 鈴木正敏、鈴木啓司、山下俊一
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-23
• [Presentation] Comprehensive pedigree analysis of cell death pathway in mammary carcilloma cell line exposed to ionizing radiation (2010)
  • Author(s): 鈴木正敏
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-09-23
• [Presentation] 放射線照射による神経幹細胞からアストロサイトへの分化誘導促進機構 (2010)
  • Author(s): 尾関あゆみ、鈴木正敏、小澤寛樹、鈴木啓司、山下俊一
  • Organizer: Neuro 2010
  • Place of Presentation: 兵庫
• [Presentation] 放射線照射がん細胞における老化様増殖停止の誘導と非アポトーシス型細胞死の系譜 (2009)
  • Author(s): 鈴木正敏、鈴木啓司、山下俊一
  • Organizer: 日本放射線影響学会第52回大会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-13
• [Presentation] 放射線照射がん細胞における老化様増殖停止の誘導と非アポトーシス型細胞死の系譜 (2009)
  • Author(s): 鈴木正敏
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 南区民文化センター(広島)
  • Year and Date: 2009-11-13
• [Presentation] がん細胞における放射線誘発細胞死の系譜 (2009)
  • Author(s): 鈴木正敏
  • Organizer: 原子爆弾後障害研究会
  • Place of Presentation: 広島国際会議場(広島)
  • Year and Date: 2009-06-07
• [Presentation] Ionizing Radiation-Induced non-ppoptotic cell death, Stress-Induced Premature Senescence (SIPS) (2009)
  • Author(s): Masatoshi Suzuki
  • Organizer: Asian Congress of Radiation Research
  • Place of Presentation: COEX(ソウル)
  • Year and Date: 2009-05-19
• [Presentation] Ionizing Radiation-induced non-apoptotic cell death, Stress-Induced Premature Senescence(SIPS) (2009)
  • Author(s): Masatoshi Suzuki, Keiji Suzuki, Shunichi Yamashita
  • Organizer: Asian Congress of Radiation Research
  • Place of Presentation: Seoul.