| Project/Area Number |
21791293
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | The University of Tokushima |
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Principal Investigator |
YOSHIKAWA Kozo The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 助教 (80448331)
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| Research Collaborator |
SHIMADA Mitsuo 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10216070)
KURITA Nobuhiro 徳島大学, 病院, 特任教授 (30335814)
IWATA Takashi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (00380022)
NISHIOKA Masanori 徳島大学, 病院, 助教 (50398020)
TAKAHASHI Akira 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (90304047)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | バクテリアルトランスロケーション / オーファジー / 腸管傷害 / オートファジー / Toll like receptor / 腸上皮セルライン / オートファゴゾーム |
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| Research Abstract |
【Background】 : This aim of this study was conducted to clarify the possible functional involvement of transcellular and paracellular route in bacterial translocation.
【Methods and Results】 : 1) Transcellular route : Ten wister rats were divided into two groups : Five were treated with irinotecan and five were not treated with irinotecan, the control group. Irinotecan treated rats were administrated irinotecan 250 mg/kg intraperitoneally on days designated 0 and 1, were then killed at 48 h after treatment, and tissues were collected for analysis. Controls were treated with a saline solution. Apoptosis in the BT group was increased and inflammatory cytokine was also increased. Large intestinal resistance of the rats was decreased. 2) Paracellular route : Same model was adopted in paracellular route study. Claudin-1 protein expression of both the small and large intestine decreased (P<0.05), occludin protein expression of the small intestine decreased (P<0.05), and occludin protein expression of the large intestine had decreasing tendency (P=0.07) in irinotecan treated rats. In irinotecan treated rats, claudin-1 mRNA of the small intestine decreased (P<0.05), claudin-1 mRNA of large intestine had a tendency to decrease (P=0.05), occludin mRNA of both small and large intestine decreased (P<0.05). 3) Autophagy : We use the Atg knock out mouse (lacked the autophagy function). Mouse was treated with irinotecan. Now we investigate the relationship between the autophagy function and bacterial translocation.
【Conclusions】 : Those findings indicated some relationship between the transcellular and paracellular route and bacterial translocation. It is possible that these mechanism prevent the bacterial translocation.
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