Comparison between neural and tumor stem cells

• Project/Area Number: 21791372
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cerebral neurosurgery
• Research Institution: Sapporo Medical University
• Principal Investigator: AKIYAMA Yukinori 札幌医科大学, 医学部, 助教 (50404653)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経幹細胞 / 腫瘍幹細胞 / がん幹細胞 / CD133 / nestin / 癌幹細胞 / 細胞培養

Research Abstract

In the comparison between neural and tumor stem cells, there was some differences such as a weakness of proliferation in the tumor stem cells. We succeeded to culture some cell lines of tumor stem cells from glioma patients. The tumor stem cells could differentiate into neuronal, glial and oligodendreoglial cell lineage which were demonstrated by immunocytochemistry. The circumference of the tumor cells which was non-tumor-stem cells might affect to proliferation of the tumor stem cells. The SIRT 1 gene known as a long life gene was appeared in the CD133 positive cells. The efficacy of the SIRT1 gene in the cells will be examined by retrieving the difference of the amount of the SIRT1 gene expression in the brain tumor stem cell in the future.