Three dimensional reconstruction of electronmicroscopic images by New electron microscopic tomography

• Project/Area Number: 21791690
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Ophthalmology
• Research Institution: Kyushu University
• Principal Investigator: HISATOMI Toshio Kyushu University, 医学研究院, 助教 (50404033)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 眼細胞生物学 / 電子顕微鏡 / 細胞死 / アポトーシス / 3次元再構築 / 電子線トモグラフィー

Research Abstract

The immunihistochemistry for electron microscopy was established for electron microscopic tomography. The automatic input of continuous serial images and 3D reconstruction from these images were examined for tomography.

Research Products

• [Journal Article] Utilizing Targeted Gene Therapy with Nanoparticles Binding Alpha v Beta 3 for Imaging and Treating Choroidal Neovascularization. (2011)
  • Author(s): Salehi-Had H, Roh MI, Giani A, Hisatomi T, Nakao S, Kim IK, Gragoudas ES, Vavvas D, Guccione S, Miller JW.
  • Journal Title: PLoS One. 6(4)
• [Journal Article] Receptor interacting protein kinases mediate retinal detachment-induced photoreceptor necrosis and compensate for inhibition of apoptosis. (2010)
  • Author(s): Trichonas G, Murakami Y, Thanos A, Morizane Y, Kayama M, Debouck CM, Hisatomi T, Miller JW, Vavvas DG.
  • Journal Title: Proc Natl Acad Sci U S A. 107(50) Pages: 21695-21700
• [Journal Article] Establishment of a new animal model of focal subretinal fibrosis that resembles disciform lesion in advanced age-related macular degeneration. (2010)
  • Author(s): Jo YJ, Sonoda KH, Oshima Y, Takeda A, Kohno R, Yamada J, Hamuro J, Yang Y, Notomi S, Hisatomi T, Ishibashi T.
  • Journal Title: Invest Ophthalmol Vis Sci. 4
• [Journal Article] Retinitis pigmentosa associated with asteroid hyalosis. (2010)
  • Author(s): Ikeda Y, Hisatomi T, Murakami Y, Miyazaki M, Kohno R, Takahashi H, Hata Y, Ishibashi T.
  • Journal Title: Retina. 30(8) Pages: 1278-1281
• [Journal Article] Histopathology of neovascular tissue from eyes with proliferative diabetic retinopathy after intravitreal bevacizumab injection. (2010)
  • Author(s): Kohno R, Hata Y, Mochizuki Y, Arita R, Kawahara S, Kita T, Miyazaki M, Hisatomi T, Ikeda Y, Aiello LP, Ishibashi T.
  • Journal Title: Am J Ophthalmol. 150(2) Pages: 223-229
• [Journal Article] An animal model of spontaneous metabolic syndrome : Nile grass rat. (2010)
  • Author(s): Noda K, Melhorn MI, Zandi S, Frimmel S, Tayyari F, Hisatomi T, Almulki L, Pronczuk A, Hayes KC, Hafezi-Moghadam A.
  • Journal Title: FASEB J. 24(7) Pages: 2443-2453
• [Journal Article] Localization of vascular adhesion protein-1 (VAP-1) in the human eye. (2010)
  • Author(s): Almulki L, Noda K, Nakao S, Hisatomi T, Thomas KL, Hafezi-Moghadam A.
  • Journal Title: Exp Eye Res. 90(1) Pages: 26-32
• [Journal Article] Pharmacological inhibition of mitochondrial membrane permeabilization for neuroprotection. (2009)
  • Author(s): Hisatomi T, Ishibashi T, Miller JW, Kroemer G.
  • Journal Title: Exp Neurol. 218(2) Pages: 347-352
• [Journal Article] Surprising up-regulation of P-selectin glycoprotein ligand-1(PSGL-1)in endotoxin-induced uveitis. (2009)
  • Author(s): Almulki L, Noda K, Amini R, Schering A, Garland RC, Nakao S, Nakazawa T, Hisatomi T, Thomas KL, Masli S, Hafezi-Moghadam A.
  • Journal Title: FASEB J. 23(3) Pages: 929-939
• [Presentation] マウス脈絡膜新生血管の蛍光走査型電子顕微鏡(FL-SEM)による観察 (2011)
  • Author(s): 納富昭司・久冨智朗
  • Organizer: 日本眼科学会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-04-16
• [Presentation] Brilliant Blue Gの細胞染色性と細胞膜透過性の検討 (2011)
  • Author(s): 久冨智朗
  • Organizer: 班会議
  • Place of Presentation: 名古屋
• [Presentation] Brilliant Blue Gの細胞染色性と細胞膜透過性の検討 (2011)
  • Author(s): 久冨智朗
  • Organizer: 日本眼科学会
  • Place of Presentation: 東京
• [Presentation] P2X7受容体遮断薬Brilliant Blue Gによる網膜神経細胞保護効果の検討 (2011)
  • Author(s): 納富昭司、久冨智朗
  • Organizer: 班会議
  • Place of Presentation: 名古屋
• [Presentation] P2X7受容体遮断薬Brilliant Blue Gによる網膜神経細胞保護効果の検討 (2011)
  • Author(s): 納富昭司、久冨智朗
  • Organizer: 日本眼科学会
  • Place of Presentation: 東京
• [Presentation] 胞状網膜剥離と浅い網膜剥離における視細胞死の比較 (2010)
  • Author(s): 久冨智朗
  • Organizer: 分子生物学研究会
  • Place of Presentation: 大阪
• [Presentation] 胞状網膜剥離と浅い網膜剥離における視細胞死の比較 (2010)
  • Author(s): 久冨智朗
  • Organizer: 日本眼科学会
  • Place of Presentation: 名古屋
• [Presentation] マウス脈絡膜新生血管の蛍光走査型電子顕微鏡(FL-SEM)による観察 (2010)
  • Author(s): 納富昭司、久冨智朗
  • Organizer: 分子生物学研究会
  • Place of Presentation: 大阪
• [Presentation] マウス脈絡膜新生血管の蛍光走査型電子顕微鏡(FL-SEM)による観察 (2010)
  • Author(s): 納富昭司、久冨智朗
  • Organizer: 日本眼科学会
  • Place of Presentation: 名古屋
• [Presentation] 網膜剥離による視細胞死;ミトコンドリア脂質二重膜透過性亢進をした分子機構 (2009)
  • Author(s): 久冨智朗
  • Organizer: 若手神経保護研究会
  • Place of Presentation: 東京
  • Year and Date: 2009-09-26
• [Presentation] 視細胞保護と血管新生抑制におけるアポトーシスの関与 (2009)
  • Author(s): 久冨智朗、納富昭司
  • Organizer: 眼創傷治癒研究会
  • Place of Presentation: 仙台
  • Year and Date: 2009-08-07
• [Presentation] 網膜剥離による視細胞死;ミトコンドリア脂質二重膜透過性亢進を介した分子機構 (2009)
  • Author(s): 久冨智朗
  • Organizer: 眼分子生物学研究会
  • Place of Presentation: 福岡
• [Presentation] 網膜剥離と視細胞死;ミトコンドリア透過性亢進を介した分子機構 (2009)
  • Author(s): 久冨智朗
  • Organizer: 北九州九州大学眼科同門会
  • Place of Presentation: 小倉
• [Presentation] 眼科血管・リンパ管研究の最前線 実験的脈絡膜血管新生の発生および退縮過程 におけるアポトーシスの発生と役割(シンポジウム) (2009)
  • Author(s): 久冨智朗
  • Organizer: 日本眼科学会
  • Place of Presentation: 東京
• [Presentation] 網膜剥離でなぜ視細胞は変性するのか? ミトコンドリアの謎(インストラクションコース) (2009)
  • Author(s): 久冨智朗
  • Organizer: 日本臨床眼科学会
  • Place of Presentation: 福岡
• [Presentation] The Bird's Eye View Analysis of Internal limiting membrane ; Cellular migration around the macular hole (2009)
  • Author(s): Toshio Hisatomi
  • Organizer: JCK meeting
  • Place of Presentation: Fukuoka
• [Remarks] ホームページ等