An investigation of tissue regeneration therapy using dental pulp-derived CD45(-), Sca-1(+) and CXCR4(+) cells transplantation.

• Project/Area Number: 21791958
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Kansai Medical University
• Principal Investigator: NAKATSUKA Ryusuke Kansai Medical University, 医学部, 助教 (90454561)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯髄幹細胞 / 分化可塑性

Research Abstract

In this study, we investigated whether these DPSCs can be prospectively isolated by FACS using bone marrow-derived mesenchymal stem cell (MSC) markers. Furthermore, characteristics of these DPSCs have been extensively investigated using FACS, immunostaining, and in vitro tissue-orientetd differentiation cultures. This study developed a novel method to prospectively isolate the dental pulp-derived mouse DPSCs and revealed that the identified DPSCs possess the potential to differentiate into multiple lineages similar to the MSCs, and these cells exist in the niche for tooth forming cells.

Research Products

• [Journal Article] Development of a High Resolution Purification Method for Precise Functional Characterization of Primitive Human Cord Blood-derived CD34-negative SCID-repopulating Cells. (2011)
  • Author(s): Ishii M, Matsuoka Y, Sasaki Y, Nakatsuka R, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Asano H, Uemura Y, Tsuji T, Fukuhara S, Sonoda Y.
  • Journal Title: Experimental hematology. 39(2) Pages: 203-213
  • Peer Reviewed
• [Journal Article] CXCL8 enhances the angiogenic activity of umbilical cord blood-derived outgrowth endothelial cells in vitro. (2011)
  • Author(s): Kimura T, Kohno H, Matsuoka Y, Murakami M, Nakatsuka R, Hase M, Yasuda K, Uemura Y, Sasaki Y, Fukuhara S, Sonoda Y.
  • Journal Title: Cell biology international. 35(3) Pages: 201-208
  • Peer Reviewed
• [Journal Article] CD16 antigen is a positive marker of peripheral blood-derived early endothelial progenitor cells. (2011)
  • Author(s): Kimura T, Kohno H, Matsuoka Y, Nakatsuka R, Sasaki Y, Fukuhara S, Sonoda Y.
  • Journal Title: International journal of hematology. 2011(1) Pages: 123-125
  • NAID: 10029532800
  • Peer Reviewed
• [Journal Article] Development of a High Resolution Purification Method for Precise Functional Characterization of Primitive Human Cord Blood-derived CD34-negative SCID-repopulating Cells (2011)
  • Author(s): Ishii M.
  • Journal Title: Experimental hematology Volume: 39 Pages: 203-213
  • Peer Reviewed
• [Journal Article] CD16 antigen is a positive marker of peripheral blood-derived early endothelial progenitor cells (2011)
  • Author(s): Kimura T.
  • Journal Title: International journal of hematology Volume: 2011 Pages: 123-125
  • Peer Reviewed
• [Journal Article] CXCL8 enhances the angiogenic activity of umbilical cord blood-derived outgrowth endothelial cells in vitro (2011)
  • Author(s): Kimura T.
  • Journal Title: Cell biology international Volume: 35 Pages: 201-208
  • Peer Reviewed
• [Journal Article] Dilazep Decreases Lipopolysaccharide-Induced Nitric Oxide and TNF-α Synthesis in RAW 264 Cells. (2010)
  • Author(s): Nakatsuka R, Nozaki T, Shinohara M, Ohura K.
  • Journal Title: Journal of pharmacological sciences. 113(3) Pages: 271-275
  • NAID: 10029889433
  • Peer Reviewed
• [Journal Article] 5-Aza-2'-deoxycytidine treatment induces skeletal myogenic differentiation of mouse dental pulp stem cells. (2010)
  • Author(s): Nakatsuka R, Nozaki T, Uemura Y, Matsuoka Y, Sasaki Y, Shinohara M, Ohura K, Sonoda Y.
  • Journal Title: Archives of Oral Biology. 55(5) Pages: 350-357
  • Peer Reviewed
• [Journal Article] In vivo dynamics of human cord blood-derived CD34(-) SCID-repopulating cells using intra-bone marrow injection. (2010)
  • Author(s): Kimura T, Matsuoka Y, Murakami M, Kimura T, Takahashi M, Nakamoto T, Yasuda K, Matsui K, Kobayashi K, Imai S, Asano H, Nakatsuka R, Uemura Y, Sasaki Y, Sonoda Y.
  • Journal Title: Leukemia. 24(1) Pages: 162-168
  • Peer Reviewed
• [Journal Article] Dilazep Decreases Lipopolysaccharide-Induced Nitric Oxide and TNF-α Synthesis in RAW 264 Cells (2010)
  • Author(s): Nakatsuka R.
  • Journal Title: Journal of pharmacological sciences Volume: 113 Pages: 271-275
  • NAID: 10029889433
  • Peer Reviewed
• [Journal Article] 5-Aza-2'-deoxycytidine treatment induces skeletal myogenic differentiation of mouse dental pulp stem cells (2010)
  • Author(s): Ryusuke Nakatsuka
  • Journal Title: Archives of Oral Biology -55 Pages: 350-357
  • Peer Reviewed
• [Journal Article] Marginal expression of CXCR4 on c-kit+Sca-1+Lineage- hematopoietic stem/progenitor cells. (2009)
  • Author(s): Sasaki Y, Matsuoka Y, Hase M, Toyohara T, Murakami M, Takahashi M, Nakatsuka R, Uemura Y, Sonoda Y.
  • Journal Title: International Journal of Hematology. 90(5) Pages: 553-560
  • NAID: 10026109792
  • Peer Reviewed
• [Journal Article] Cytokine-dependent modification of IL-12p70 and IL-23 balance in dendritic cells by ligand activation of Valpha24 invariant NKT cells. (2009)
  • Author(s): Uemura Y, Liu TY, Narita Y, Suzuki M, Nakatsuka R, Araki T, Matsumoto M, Iwai LK, Hirosawa N, Matsuoka Y, Murakami M, Kimura T, Hase M, Kohno H, Sasaki Y, Ichihara Y, Ishihara O, Kikuchi H, Sakamoto Y, Jiao SC, Senju S, Sonoda Y.
  • Journal Title: Journal of Immunology. 183(1) Pages: 201-208
  • Peer Reviewed
• [Journal Article] Cytokine-Dependent Modification of IL-12p70 and IL-23 Balance in Dendritic Cells by Ligand Activation of Vα24 Invariant NKT Cells (2009)
  • Author(s): Yasushi Uemura
  • Journal Title: The Journal of Immunology 183 Pages: 201-208
  • Peer Reviewed
• [Presentation] Prospective isolation of mouse Sca-1^+PDGFRα^+ dental pulp stem cells (DPSCs) existing in the tooth forming niche. (2010)
  • Author(s): 中塚隆介
  • Organizer: 第9回幹細胞シンポジウム
  • Place of Presentation: 兵庫県、淡路夢舞台国際会議場
  • Year and Date: 2010-05-13
• [Presentation] Prospective isolation of mouse Sca-1^+PDGFRα^+ dental pulp stem cells (DPSCs) existing in the tooth forming niche (2010)
  • Author(s): 中塚隆介
  • Organizer: 第9回幹細胞シンポジウム
  • Place of Presentation: 淡路夢舞台国際会議場
  • Year and Date: 2010-05-13
• [Presentation] Sca-1陽性、PDGFRα陽性マウス歯髄幹細胞の予期的分取と特性の解析 (2010)
  • Author(s): 中塚隆介
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島県、広島国際会議場
  • Year and Date: 2010-03-19
• [Presentation] Sca-1陽性、PDGFRα陽性マウス歯髄幹細胞の予期的分取と特性の解析 (2010)
  • Author(s): 中塚隆介
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2010-03-19
• [Presentation] Multipotent mouse Sca-1 and PDGFRα double positive DPSCs exist in the tooth forming niche. (2009)
  • Author(s): 中塚隆介
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 神奈川県、パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] Multipotent mouse Sca-1 and PDGFRα double positive DPSCs exist in the tooth forming niche (2009)
  • Author(s): 中塚隆介
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] Prospective isolation of mouse Sca-1+PDGFRα^+ dental pulp stem cells (DPSCs) existing in the tooth forming niche. (2009)
  • Author(s): 中塚隆介
  • Organizer: 第8回幹細胞シンポジウム
  • Place of Presentation: 東京都、泉ガーデンギャラリー
  • Year and Date: 2009-05-15
• [Presentation] Prospective isolation of mouse Sca-1^+PDGFRα^+ dental pulp stem cells(DPSCs)existing in the tooth forming niche (2009)
  • Author(s): 中塚隆介
  • Organizer: 第8回幹細胞シンポジウム
  • Place of Presentation: 泉ガーデンギャラリー
  • Year and Date: 2009-05-15