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The presence of specific oral streptococci and hyperfunction of innate immune responce are causative factors for childhood allergy diseases in oral area.

Research Project

Project/Area Number 21792069
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Orthodontic/Pediatric dentistry
Research InstitutionTokyo Dental College (2010)
Osaka University (2009)

Principal Investigator

SAKURAI Atsuo  Tokyo Dental College, 歯学部, 助教 (90431759)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords小児歯科学 / 口腔レンサ球菌 / 上皮細胞 / 自然免疫 / レンサ球菌 / 口腔細菌叢 / 口腔最近叢
Research Abstract

Streptococcus sanguinis is a predominant bacterium in the human oral cavity. Some S. sanguinis strains are considered benetifical because they are reported to attenuate imflamatory responses caused by periodontopathogens. We identified S. sanguinis strain SK36 had an ability to adhere to and invade into human epithelial cells. Pili of S. sanguinis was an important factor to bind to fibronectin, and such binding ability to ECM proteins contribute to such invasion. SK36 enhances the immune responses of host cells such as overexpression of inflammatory cytokines and beta-defensin. A standard S. sanguinis strain ATCC10556 and pili defective mutant of SK36 failed to invade into epithelial cells and to stimulate the immune response. Taken together, our results suggest the possible involvement of pili in the pathogenesis of infective endocarditis, oral allergic diseases, and disorder of immune system.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (26 results)

All 2011 2010 2009

All Journal Article (14 results) (of which Peer Reviewed: 12 results) Presentation (11 results) Book (1 results)

  • [Journal Article] Pili of oral Streptococcus sanguinis bind to salivary amylase and promote the biofilm formation.2011

    • Author(s)
      N.Okahashi, M.Nakata, Y.Terao, R.Isoda, A.Sakurai, T.Sumitomo, M.Yamaguchi, R.K.Kimura, E.Oiki, S.Kawabata, T.Ooshima.
    • Journal Title

      Microbial Pathogenesis. 50

      Pages: 148-154

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two cases of less invasive treatment for radicular cyst in primary dentition.2011

    • Author(s)
      A.Sakurai, et al.
    • Journal Title

      Pediatric Dental Journal

      Volume: (In press)

    • NAID

      10028257262

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pili of oral Streptococcus sanguinis bind to salivary amylase and promote the biofilm formation.2011

    • Author(s)
      N.Okahashi, et al.
    • Journal Title

      Microbial Pathogenesis

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] (1)小児齲蝕症(授乳と齲蝕の関係をどのように指導するか・最新の齲蝕病因論・哺乳齲蝕とその対応)(2)歯冠修復処置(乳臼歯隣接面コンポジットレジン修復の適応と実際)2011

    • Author(s)
      桜井敦朗(分担執筆)
    • Journal Title

      デンタルダイヤモンド春期特別号「小児歯科はいま,成育医療へ」

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
  • [Journal Article] The specific behavior of intracellular Streptococcus pyogenes undergone the autophagic degradation is associated with bacterial streptolysin O and host small G proteins Rab5 and Rab7.2010

    • Author(s)
      A.Sakurai, F.Maruyama, J.Funao, T.Nozawa, C.Aikawa, N.Okahashi, S.Shintani, S.Hamada, T.Ooshima, I.Nakagawa.
    • Journal Title

      Journal of Biological Chemistry. 285(29)

      Pages: 22666-22675

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Histopathological characteristics of primary teeth in pre-term very low birth-weight child : Case report.2010

    • Author(s)
      J.Funao-Tanigawa, T.Onishi, A.Sakurai, A.Kojima, H.Homma, T.Ooshima, M.Rakugi, S.Shintani.
    • Journal Title

      Pediatric Dental Journal. 20(2)

      Pages: 182-185

    • NAID

      10027485966

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pili of oral Streptococcus sanguinis bind to fibronectin and contribute to cell adhesion.2010

    • Author(s)
      N.Okahashi, N.Masanobu, A.Sakurai, T.Terao, T.Hoshino, M.Yamaguchi, R.Isoda, T.Sumitomo, K.Nakano, S.Kawabata, T.Ooshima.
    • Journal Title

      Biochemical and Biophysical Research Communications. 391(2)

      Pages: 1192-1196

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Histopathological characteristics of primary teeth in pre-term very lowbirth-weight child : Case report.2010

    • Author(s)
      J.Funao-Tanigawa, et al.
    • Journal Title

      Pediatric Dental Journal

      Volume: 20 Pages: 182-185

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The specific behavior of intracellular Streptococcus pyogenes undergone the autophagic degradation is associated with bacterial streptolysin O and host small G proteins Rab5 and Rab7.2010

    • Author(s)
      A.Sakurai, et al.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 22666-22675

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pili of oral Streptococcus sanguinis bind to fibronectin and contribute to cell adhesion.2010

    • Author(s)
      N.Okahashi
    • Journal Title

      Biochem Biophys Res Commun. 391

      Pages: 1192-1196

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative genomic analyses of Streptococcus mutans provide insights into chromosomal shuffling and species-specific content.2009

    • Author(s)
      F.Maruyama, M.Kobata, K.Kurokawa, K.Nishida, A.Sakurai, K.Nakano, R.Nomura, S.Kawabata, T.Ooshima, K.Nakai, M.Hattori, S.Hamada, I.Nakagawa.
    • Journal Title

      BMC genomics. 10(Aug.5)

      Pages: 358-378

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cost effective DNA sequencing and template preparation from bacterial colonies and plasmids.2009

    • Author(s)
      F.Maruyama, T.Nozawa, C.Aikawa, A.Sakurai, I.Nakagawa.
    • Journal Title

      Journal of bioscience and bioengineering. 107(4)

      Pages: 471-473

    • NAID

      110007225791

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cost effective DNA sequencing and template preparation from bacteri alcolonies and plasmids.2009

    • Author(s)
      F.Maruyama
    • Journal Title

      Journal of bioscience and bioengineering 107

      Pages: 471-473

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative genomic analyses of Streptococcus mutans provide insights into chromosomal shuffling and species-specific content.2009

    • Author(s)
      F.Maruyama
    • Journal Title

      BMC genomics 10

      Pages: 358-3783

    • Related Report
      2009 Annual Research Report
  • [Presentation] 母乳と齲蝕を細菌学から考察する2010

    • Author(s)
      桜井敦朗
    • Organizer
      千葉県小児歯科医会セミナー(招待講演)
    • Place of Presentation
      千葉
    • Year and Date
      2010-08-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 母乳と齲蝕を細菌学から考察する2010

    • Author(s)
      桜井敦朗
    • Organizer
      千葉県小児歯科医会セミナー
    • Place of Presentation
      千葉(招待講演)
    • Year and Date
      2010-08-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 上顎骨腫瘍摘出術後に長期経過観察を行った一例2010

    • Author(s)
      桜井敦朗, 大川玲奈, 新谷誠康, 大嶋隆
    • Organizer
      第48回日本小児歯科学会
    • Year and Date
      2010-05-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 上顎骨腫瘍摘出術後に長期経過観察を行った1例2010

    • Author(s)
      桜井敦朗, ら
    • Organizer
      第48回日本小児歯科学会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-05-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 口腔レンサ球菌S.sanguinis線毛piliの機能2010

    • Author(s)
      岡橋暢夫, 中田匡宣, 桜井敦朗, 寺尾豊, 山口雅也, 星野倫範, 川端重忠, 大嶋隆
    • Organizer
      第83回日本細菌学会総会
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Streptococcus anginosusの遺伝系統学的分類および宿主病原性に関する解析2010

    • Author(s)
      桜井敦朗, 星野倫範, 岡橋暢夫, 森有可, 丸山史人, 藤原卓, 大嶋隆
    • Organizer
      第83回日本細菌学会総会
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Streptococcus angionosusの遺伝系統学的分類および宿主病原性に関する解析2010

    • Author(s)
      桜井敦朗
    • Organizer
      日本細菌学会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 口腔領域レンサ球菌による宿主上皮細胞への付着侵入と炎症の誘導2009

    • Author(s)
      桜井敦朗, 岡橋暢夫, 中田匡宣, 寺尾豊, 丸山史人, 川端重忠, 大嶋隆
    • Organizer
      第18回Lancefieldレンサ球菌研究会
    • Year and Date
      2009-06-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 口腔領域レンサ球菌による宿主上皮細胞への付着侵入と炎症の誘導2009

    • Author(s)
      桜井敦朗
    • Organizer
      Lancefieldレンサ球菌研究会
    • Place of Presentation
      福岡
    • Year and Date
      2009-06-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 石灰化が I 型コラーゲンの発現に与える影響2009

    • Author(s)
      鎌倉尚史、小畑充彦, 大川玲奈, 桜井敦朗, 大嶋隆
    • Organizer
      第47回日本小児歯科学会
    • Year and Date
      2009-05-15
    • Related Report
      2010 Final Research Report
  • [Presentation] 口腔レンサ球菌の宿主上皮細胞への付着侵入能と宿主免疫応答の解析2009

    • Author(s)
      桜井敦朗
    • Organizer
      日本小児歯科学会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-15
    • Related Report
      2009 Annual Research Report
  • [Book] 小児歯科はいま,成育医療へ((1)小児齲蝕症(授乳と齲蝕の関係をどのように指導するか・最新の齲蝕病因論・哺乳齲蝕とその対応).(2)歯冠修復処置(乳臼歯隣接面コンポジットレジン修復の適応と実際).デンタルダイヤモンド春期特別号)2011

    • Author(s)
      桜井敦朗(分担執筆)
    • Total Pages
      4
    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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