Real-time monitoring of single biomolecular interaction under microfluidic control

• Project/Area Number: 21810012
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Nagoya University
• Principal Investigator: ONOSHIMA Daisuke Nagoya University, 工学研究科, 研究員 (40510219)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,561,000 (Direct Cost: ¥1,970,000、Indirect Cost: ¥591,000); Fiscal Year 2010: ¥1,222,000 (Direct Cost: ¥940,000、Indirect Cost: ¥282,000); Fiscal Year 2009: ¥1,339,000 (Direct Cost: ¥1,030,000、Indirect Cost: ¥309,000)
• Keywords: 1分子計測(SMD) / マイクロ・ナノデバイス / ナノバイオ / 生物物理 / 生体分子

Research Abstract

Single-molecule, real-time intermolecular interaction analysis was achieved by microfluidic control. The method enabled tracking and analysis of biomolecules in a physiologically natural state. Further applications of our method will provide new understanding of molecular mechanisms by which DNA and protein perform their diverse biological functions in cells.

Research Products

• [Journal Article] Tracking Degradations of Single DNA and Protein Molecules in Fluid (2011)
  • Author(s): D.Onoshima, N.Kaji, M.Tokeshi, Y.Baba
  • Journal Title: Biophysical Journal Vol.100
  • Peer Reviewed
• [Journal Article] Tracking Degradations of Single DNA and Protein Molecules in Fluid (2011)
  • Author(s): Daisuke Onoshima, et al
  • Journal Title: Biophysical Journal Volume: Vol.100
  • Peer Reviewed
• [Journal Article] TRACKING OF SINGLE DNA AND PROTEIN MOLECULES UNDERGOING ENZYMATIC DEGRADATION IN FLUID (2010)
  • Author(s): D.Onoshima, N.Kaji, M.Tokeshi, Y.Baba
  • Journal Title: Proceedings of μTAS 2010 Vol.1 Pages: 1439-1441
  • Peer Reviewed
• [Journal Article] TRACKING OF SINGLE DNA AND PROTEIN MOLECULES UNDERGOING ENZYMATIC DEGRADATION IN FLUID (2010)
  • Author(s): Daisuke Onoshima, et al
  • Journal Title: The Proceedings of μTAS 2010 Volume: Vol.1 Pages: 1439-1441
  • Peer Reviewed
• [Journal Article] A SIMPLE AND LOW-COST TECHNIQUE FOR FABRICATING MICROCHANNELS AND MICROSTRUCTURES WITH HIGH ASPECT RATIO (2009)
  • Author(s): D.Onoshima, J.Wang, M.Aki, K.Arinaga, N.Kaji, M.Tokeshi, S.Fujita, N.Yokoyama, Y.Baba
  • Journal Title: The Proceedings of μTAS 2009 Vol.2 Pages: 1587-1589
  • Peer Reviewed
• [Journal Article] A SIMPLE AND LOW-COST TECHNIQUE FOR FABRICATING MICRO CHANNELS AND MICROSTRUCTURES WITH HIGH ASPECT RATIO (2009)
  • Author(s): Daisuke Onoshima, et al
  • Journal Title: The Proceedings of μTAS 2009 Vol. 2 Pages: 1587-1589
  • Peer Reviewed
• [Presentation] マイクロ流体制御下におけるプロテアーゼ反応の分子的検出 (2011)
  • Author(s): 小野島大介, 他
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-28
• [Presentation] マイクロ流体制御下におけるプロテアーゼ反応の分子的検出 (2011)
  • Author(s): 小野島大介・加地範匡・渡慶次学・馬場嘉信
  • Organizer: 日本化学会第91春季年会
  • Place of Presentation: 講演予稿集
  • Year and Date: 2011-03-11
• [Presentation] Tracking Degradations of Single DNA and Protein Molecules in Fluid (2011)
  • Author(s): 小野島大介, 加地範匡, 渡慶次学, 馬場嘉信
  • Organizer: Biophysical Society 55th Annual Meeting
  • Place of Presentation: Baltimore Convention Center, Baltimore, Maryland, USA
  • Year and Date: 2011-03-06
• [Presentation] Tracking Degradations of Single DNA and Protein Molecules in Fluid (2011)
  • Author(s): Daisuke Onoshima, et al
  • Organizer: Biophysical Society 55^<th> Annual Meeting
  • Place of Presentation: Baltimore Convention Center, Baltimore, Maryland, USA
  • Year and Date: 2011-03-06
• [Presentation] マイクロ流体デバイスを利用した1分子生物学への新しいアプローチ、4大学ナノ・マイクロファブリケーションコンソーシアム 拠点形成シンポジウム (2011)
  • Author(s): 小野島大介
  • Organizer: 「グリーンイノベーション・ライフイノベーションを促進する 材料、計測、ファブリケーション、デバイス技術
  • Place of Presentation: 川崎市産業振興会館 1F メインホール
  • Year and Date: 2011-03-04
• [Presentation] マイクロ流体デバイスを利用した1分子生物学への新しいアプローチ (2011)
  • Author(s): 小野島大介
  • Organizer: 4大学ナノ・マイクロファブリケーションコンソーシアム拠点形成シンポジウム「グリーンイノベーション・ライフイノベーションを促進する材料、計測、ファブリケーション、デバイス技術」
  • Place of Presentation: 川崎市産業振興会館1Fメインホール
  • Year and Date: 2011-03-04
• [Presentation] Optical Tracking of Single Biomolecules Undergoing EnzymaticDegradations (2011)
  • Author(s): 小野島大介, 加地範匡, 渡慶次学, 馬場嘉信
  • Organizer: InternationalSymposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules(ISSMA2011)
  • Place of Presentation: Kyoto International Conference Center (ICC Kyoto), Kyoto, Japan
  • Year and Date: 2011-01-24
• [Presentation] Optical Tracking of Single Biomolecules Undergoing Enzymatic Degradations (2011)
  • Author(s): Daisuke Onoshima, et al
  • Organizer: International Symposium : Advanced Science and Technology for Single Molecular Analysis of DNA and Related Molecules (ISSMA 2011)
  • Place of Presentation: Kyoto International Conference Center (ICC Kyoto), Kyoto, Japan
  • Year and Date: 2011-01-24
• [Presentation] A molecular tracking ofprotein-DNA interactions under microfluidic control (2010)
  • Author(s): 小野島大介, 加地範匡, 渡慶次学, 馬場嘉信
  • Organizer: The International Chemical Congress ofPacific Basin Societies(Pacifichem2010)
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, USA
  • Year and Date: 2010-12-15
• [Presentation] A molecular tracking of protein-DNA interactions under microfluidic control (2010)
  • Author(s): Daisuke Onoshima, et al
  • Organizer: The International Chemical Congress of Pacific Basin Sacieties (Pacifichem 2010)
  • Place of Presentation: Hawaii Convention Center, Honolulu, Hawaii, USA
  • Year and Date: 2010-12-15
• [Presentation] プロテアーゼ反応の分子的リアルタイムトラッキング (2010)
  • Author(s): 小野島大介・加地範匡・渡慶次学・馬場嘉信
  • Organizer: 第22回化学とマイクロ・ナノシステム研究会(22nd CHEMINAS)
  • Place of Presentation: 名古屋大学鶴舞キャンパス
  • Year and Date: 2010-11-18
• [Presentation] TRACKING OF SINGLE DNA AND PROTEIN MOLECULES UNDERGOING ENZYMATIC DEGRADATION IN FLUID (2010)
  • Author(s): 小野島大介, 加地範匡, 渡慶次学, 馬場嘉信
  • Organizer: The 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2010)
  • Place of Presentation: Martiniplaza, Groningen, Nertherlands
  • Year and Date: 2010-10-06
• [Presentation] A molecular detection of nucleolytic degradation under microfluidic control (2010)
  • Author(s): 小野島大介, 加地範匡, 渡慶次学, 馬場嘉信
  • Organizer: International Symposium on Microchemistry and Microsystems 2010 (ISMM 2010)
  • Place of Presentation: Hong Kong University of Science and Technology (HKUST), Hong Kong
  • Year and Date: 2010-05-28
• [Presentation] マイクロ流体制御下における核酸分解酵素反応の分子的検出 (2010)
  • Author(s): 小野島大介、加地範匡、渡慶次学、馬場嘉信
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学本部キャンパス
  • Year and Date: 2010-03-27
• [Presentation] マイクロ流体制御下における核酸分解酵素反応の分子的検出 (2010)
  • Author(s): 小野島大介, 他
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 近畿大学本部キャンパス(大阪府)
  • Year and Date: 2010-03-27
• [Presentation] A Combination of a Single-Molecule Tracking and Microfluidic Flow System Enables an Analysis of Ongoing Processes of Enzymatic Reaction (2009)
  • Author(s): 小野島大介
  • Organizer: BIT's World Congress of Gene-2009 (WCG-2009)
  • Place of Presentation: Foshan Golden Sun Hotel (FGSH), Foshan, China
  • Year and Date: 2009-12-05
• [Presentation] A Combination of a Single-Molecule Tracking and Microfluidic Flow System Enables an Analysis of Ongoing Processes of Enzymatic Reaction (2009)
  • Author(s): Daisuke Onoshima
  • Organizer: BIT's World Congress of Gene-2009(WCG-2009)
  • Place of Presentation: Foshan Golden Sun Hotel(FGSH), Foshan, China
  • Year and Date: 2009-12-05
• [Presentation] ヌクレアーゼ相互作用のマイクロ流体制御と1分子リアルタイム計測 (2009)
  • Author(s): 小野島大介・加地範匡・渡慶次学・馬場嘉信
  • Organizer: 第40回中部化学関係学協会支部連合秋季大会
  • Place of Presentation: 岐阜大学工学部
  • Year and Date: 2009-11-08
• [Presentation] A SIMPLE AND LOW-COST TECHNIQUE FOR FABRICATING MICROCHANNELS AND MICROSTRUCTURES WITH HIGH ASPECT RATIO (2009)
  • Author(s): D.Onoshima, J.Wang, M.Aki, K.Arinaga, 加地範匡, 渡慶次学, S.Fujita, N.Yokoyama, 馬場嘉信
  • Organizer: The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS2009)
  • Place of Presentation: International Convention Center at Jeju Istand (ICC Jeju), Korea
  • Year and Date: 2009-11-04
• [Presentation] A SIMPLE AND LOW-COST TECHNIQUE FOR FABRICATING MICRO CHANNELS AND MICROSTRUCTURES WITH HIGH ASPECT RATIO (2009)
  • Author(s): Daisuke Onoshima, et al
  • Organizer: The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences(μTAS 2009)
  • Place of Presentation: International Convention Center at Jeju Istand(ICC Jeju), Korea
  • Year and Date: 2009-11-04
• [Book] 『化学フロンティア22生命現象を理解する分子ツール イメージングから生体機能解析まで』IV部19章(浜地格・二木史朗編) (2010)
  • Author(s): 小野島大介・馬場嘉信
  • Publisher: 化学同人
• [Book] 化学フロンティア22生命現象を理解する分子ツールイメージングから生体機能解析まで(浜地格・二木史朗編)(IV部19章) (2010)
  • Author(s): 小野島大介、馬場嘉信
  • Publisher: 化学同人
• [Patent(Industrial Property Rights)] 分析デバイス、分析デバイスの製造方法及び分析デバイスを用いる分析装置 (2009)
  • Inventor(s): 小野島大介・加地範匡・渡慶次学・馬場嘉信
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Number: 2009-251536
  • Filing Date: 2009-10-30
• [Patent(Industrial Property Rights)] 分析デバイス、分析デバイスの製造方法及び分析デバイスを用いる分析装置 (2009)
  • Inventor(s): 小野島大介, 他
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Number: 2009-251536
  • Filing Date: 2009-10-30