| Project/Area Number |
21860057
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka University |
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Principal Investigator |
OJIMA Yoshihiro Osaka University, 大学院・基礎工学研究科, 助教 (20546957)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000)
Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
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| Keywords | 大腸菌 / バイオフィルム / アクチンタンパク質 / 鞭毛 / アクチン様タンパク質 / 運動性 / 遺伝子発現 / ストレス応答 / 接着性 / 遊走性 / 遺伝子 / 薬剤耐性力 / 抗酸化剤 |
|---|
| Research Abstract |
The knockout of yggE gene, which encodes the actin-like protein of E. coli, showed the strong attachment of cells on the solid surface (such as polyvinyl chloride (PVC) and polypropylenes (PP)) compared with the wild type of E. coli. As a mechanism of attachment, it was confirmed that the expression of flagella-related genes in yggE knockout mutant were 10 times higher than that of wild type and motility rate increased by three times. In addition, the observation of attached cells of yggE knockout mutant by using the scanning electron microscope revealed that the number of flagellum increased by several times compared with the wild strain, and those flagellums connected the cell with the solid surface or the cells each other. These results suggest that the actin-like protein of E. coli is deeply related to the flagellum and the biofilm formation.
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