Cellular systems of prion maintenance

• Project/Area Number: 21870009
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: KURAHASHI Hiroshi The University of Tokyo, 医科学研究所, 助教 (60508357)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: プリオン / アミロイド / 蛋白質 / 酵母 / 感染症 / 脳神経疾患 / タンパク質 / 凝集

Research Abstract

The yeast Saccharomyces cerevisiae has prions that are infectious proteins. In this study, we isolated single-amino-acid mutations in rnq1 which are defective in the stable maintenance of the [RNQ^+] prion and inhibit [PSI^+] propagation. The majority of these mutated residues are mapped to the surface of contiguous α-helices of the nonprion domain of Rnq1, suggesting its involvement in interactions with a prion or a factor necessary for prion development.

Research Products

• [Journal Article] [PSI+] aggregate enlargement in rnq1 non-prion domain mutants, leading to a loss-of-prion in yeast. (2011)
  • Author(s): Hiroshi Kurahashi, Chan-Gi Pack, Shoichiro Shibata, Keita Oishi, Yasushi Sako, Yoshikazu Nakamura
  • Journal Title: Genes Cells 16 Pages: 576-589
  • Peer Reviewed
• [Journal Article] [PSI%+] aggregate enlargement in rnql nonprion domain mutants, leading to a loss of prion in yeast (2011)
  • Author(s): Hiroshi Kurahashi, Chan-Gi Pack, Shoichiro Shibata, Keita Oishi, Yasushi Sako, Yoshikazu Nakamura
  • Journal Title: Genes to Cells Volume: (in press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Localization of prion-destabilizing mutations in the N-terminal non-prion domain of Rnq1 in Saccharomyces cerevisiae. (2009)
  • Author(s): Shoichiro Shibata, Hiroshi Kurahashi, Yoshikazu Nakamura
  • Journal Title: Prion 3 Pages: 250-258
  • Peer Reviewed
• [Journal Article] Localization of prion-destabilizing mutations in the N-terminal non-prion domain of Rnq1 in Saccharomyces cerevisiae (2009)
  • Author(s): Shoichiro Shibata, Hiroshi Kurahashi, Yoshikazu Nakamura
  • Journal Title: Prion VOL.3 Pages: 250-258
  • Peer Reviewed
• [Presentation] 出芽酵母のプリオン動態におけるQ/N-rich領域の"二重人格性" (2010)
  • Author(s): 大石圭太,倉橋洋史,中村義一
  • Organizer: BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] LOCALIZATION OF AMYLOID-DESTABILIZING MUTATIONS IN THE N-TERMINAL DOMAIN OF RNQ1 IN SACCHAROMYCES CEREVISIAE. (2010)
  • Author(s): Hiroshi Kurahashi, Shoichiro Shibata, Yoshikazu Nakamura
  • Organizer: The 4th International Symposium of Molecular Science of Fluctuations toward Biological Functions
  • Place of Presentation: 大津
  • Year and Date: 2010-12-01
• [Presentation] LOCALIZATION OF AMYLOID-DESTABILIZING MUTATIONS IN THE N-TERMINAL DOMAIN OF RNQ1 IN SACCHAROMYCES CEREVISIAE (2010)
  • Author(s): Hiroshi Kurahashi, Shoichiro Shibata, Yoshikazu Nakamura
  • Organizer: The 4th International Symposium of Molecular Science of Fluctuations toward Biological Functions
  • Place of Presentation: 大津 ピアザ淡海
  • Year and Date: 2010-12-01
• [Presentation] A novel prion antagonist in yeast. (2010)
  • Author(s): Keita Oishi, Hiroshi Kurahashi, Yoshikazu Nakamura
  • Organizer: A FEBS Advanced Lecture Course : Analysis and Engineering of Biomolecular Systems
  • Place of Presentation: Island of Spetes, Greece
  • Year and Date: 2010-09-13
• [Presentation] The mechanism of yeast prion elimination. (2010)
  • Author(s): Chie Arai, Hiroshi Kurahashi, Keita Oishi, Yoshikazu Nakamura
  • Organizer: A FEBS Advanced Lecture Course : Analysis and Engineering of Biomolecular Systems
  • Place of Presentation: Island of Spetes, Greece
  • Year and Date: 2010-09-13
• [Presentation] Alterations in predicted α-helices of non-prion domains of Rnq1 affect homologous and heterologous prion propagations in yeast. (2010)
  • Author(s): Hiroshi Kurahashi, Shoichiro Shibata, Yoshikazu Nakamura
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: 奈良
  • Year and Date: 2010-09-13
• [Presentation] Alterations in predicted α-helices of non-prion domains of Rnql affect homologous and heterologous prion propagations in yeast (2010)
  • Author(s): Hiroshi Kurahashi, Shoichiro Shibata, Yoshikazu Nakamura
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: 奈良 ホテル日航奈良
  • Year and Date: 2010-09-13
• [Presentation] Rnq1の非プリオンドメインの変異は、同種と異種のプリオン維持阻害を引き起こす (2010)
  • Author(s): 倉橋洋史,柴田勝一郎,中村義一
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-18
• [Presentation] Rnq1の非プリオンドメインの変異は、同種と異種のプリオン維持阻害を引き起こす (2010)
  • Author(s): 倉橋洋史, 柴田勝一郎, 中村義一
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌,札幌コンベンションセンター
  • Year and Date: 2010-06-18
• [Presentation] Localization of prion-destabilizing mutations in the N-terminal non-prion domain of Rnq1 in Saccharomyces cerevisiae. (2009)
  • Author(s): Shoichiro Shibata, Hiroshi Kurahashi, Yoshikazu Nakamura
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10