Analysis of translesion synthesis at replication fork in vitro

• Project/Area Number: 21870023
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: FURUKOHRI Asako Nara Institute of Science and Technology, バイオサイエンス研究科, 助教 (90546293)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,743,000 (Direct Cost: ¥2,110,000、Indirect Cost: ¥633,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: DNA複製 / DNA修復 / 突然変異 / 大腸菌

Research Abstract

The aim of this study is finding a new role of translesion DNA polymerase in a mechanism for chromosomal DNA replication. Using E.coli in vitro DNA replication system, we found that the polymerase switching from replicative polymerase Pol III to translesion polymerase Pol IV was stimulated by the action of Pol IV.

Research Products

• [Journal Article] Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication. (2011)
  • Author(s): Kundu LR, Seki M, Watanabe N, Murofushi H, Furukohri A, Waga S, Score AJ, Blow JJ, Horikoshi M, Enomoto T, Tada S.
  • Journal Title: Biochim Biophys Acta. 1813(6) Pages: 1129-1136
  • Peer Reviewed
• [Journal Article] Biphasic chromatin binding of histone chaperone FACT during eukaryotic chromatin DNA replication. (2011)
  • Author(s): Kundu LR
  • Journal Title: Biochim Biophys Acta. Volume: Jan
  • Peer Reviewed
• [Journal Article] Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex. (2011)
  • Author(s): Kundu LR
  • Journal Title: Nucleic Acids Res. Volume: 38 Pages: 5409-18
  • Peer Reviewed
• [Journal Article] Deregulated Cdc6 inhibits DNA replication and suppresses Cdc7-mediated phosphorylation of Mcm2-7 complex. (2010)
  • Author(s): Kundu LR, Kumata Y, Kakusho N, Watanabe S, Furukohri A, Waga S, Seki M, Masai H, Enomoto T, Tada S.
  • Journal Title: Nucleic Acids Res. 38(16) Pages: 5409-5418
  • Peer Reviewed
• [Presentation] Overproduction of Escherichia coli DNA Polymerase DinB(Pol IV) Inhibits Replication Fork Progression and is Lethal. (2010)
  • Author(s): Uchida K, Furukohri A, Shinozaki Y.Mori T, Ogawara D, Kanaya S, Nohmi T, Maki H, Akiyama M
  • Organizer: The 57th NIBB Conference THE DYNAMIC GENOME
  • Place of Presentation: Okazaki Conference Center, Okazaki, Aichi, Japan
  • Year and Date: 2010-10-14
• [Book] Elsevier (2011)
  • Author(s): Hisaji Maki, Asako Furukohri
  • Publisher: The Encyclopedia of Biological Chemistry second edition(in pres)