adipogenesis and chemical biology
| Project/Area Number |
21890013
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Metabolomics
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| Research Institution | Tohoku University |
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Principal Investigator |
OKAMURA Masashi Tohoku University, 大学院・医学系・研究科, 助手 (60547397)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥1,235,000 (Direct Cost: ¥950,000、Indirect Cost: ¥285,000)
Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
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| Keywords | 低酸素 / PAI-I / 脂肪細胞分化 / PHD / ホモfloxマウス / siRNA / 7xHRE人工レポーター / MTSアッセイ / 分解性検討試験 / 3T3-L1 / レトロウイルス / 低酸素シグナル / ルシフェラーゼアッセイ |
|---|
| Research Abstract |
Overexpression of PHD1,2,3 and 4 or siRNA-mediated knockdown of PHD1 and 2 did not affect 3T3-L1 adipogenesis. We successfully transferred and set up Phd1^<ff>Phd2^<ff>Phd3^<ff>, Phd2^<ff>, Phd3^<f+>and Phd2^<ff>Rosa26^<+/CreERT2>mice from Connecticut University. By using 7 x HRE artificial promoter, we screened various compounds and founded new compounds equivalent to cobalt.
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Report
(3 results)
Research Products
(62 results)
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[Journal Article] The peroxisome proliferator-activated receptor gamma/ retinoid X receptor alpha heterodimer targets the histone modification enzyme PR-Set7/Setd8 gene and regulates adipogenesis through a positive feedback loop.2009
Author(s)
Wakabayashi K, Okamura M, Tsutsumi S, Nishikawa N S, Tanaka T, Sakakibara I, Kitakami J, Ihara S, Hashimoto Y, Hamakubo T, Kodama T, Aburatani H, Sakai J.
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Journal Title
Mol Cell Biol. 29(13)
Pages: 3544-55
Related Report
Peer Reviewed
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[Journal Article] COUP-TFII acts downstream of Wnt/beta-catenin signal to silence PPARgamma gene expression and repress adipogenesis.2009
Author(s)
Okamura M, Kudo H, Wakabayashi K, Tanaka T, Nonaka A, Uchida A, Tsutsumi S, Sakakibara I, Naito M, Osborne TF, Hamakubo T, Ito S, Aburatani H, Yanagisawa M, Kodama T, Sakai J.
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Journal Title
Proc Natl Acad Sci U S A. 106(14)
Pages: 5819-24
Related Report
Peer Reviewed
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[Journal Article] The peroxisome proliferator-activated receptor gamma/retinoid X receptor alpha heterodimer targets the histone modification enzyme PR-Set7/Setd8 gene and regulates adipogenesis through a positive feedback loop.2009
Author(s)
Wakabayashi Ken-ichi, Okamura Masashi, Tsutsumi Shuichi, Nishikawa Naoko S, Tanaka Toshiya, Sakakibara Iori, Kitakami Jun-ichi, Ihara Sigeo, Hashimoto Yuichi, Hamakubo Takao, Kodama Tatsuhiko, Aburatani Hiroyuki, Sakai Juro
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Journal Title
Mol Cell Biol 29
Pages: 3544-3555
Related Report
Peer Reviewed
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[Journal Article] COUP-TFII acts downstream of Wnt/beta-catenin signal to silence PPARgamma gene expression and repress adipogenesis.2009
Author(s)
Okamura Masashi, Kudo Hiromi, Wakabayashi Ken-ichi, Tanaka Toshiya, Nonaka Aya, Uchida Aoi, Tsutsumi Shuichi, Sakakibara Iori, Naito Makoto, Osborne Timothy F, Hamakubo Takao, Ito Sadayoshi, Aburatani Hiroyuki, Yanagisawa Masashi, Kodama Tatsuhiko, Sakai Juro
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Journal Title
Proc Natl Acad Sci USA 106
Pages: 5819-5824
Related Report
Peer Reviewed
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