| Project/Area Number |
21890013
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Metabolomics
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| Research Institution | Tohoku University |
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Principal Investigator |
OKAMURA Masashi Tohoku University, 大学院・医学系・研究科, 助手 (60547397)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥1,235,000 (Direct Cost: ¥950,000、Indirect Cost: ¥285,000)
Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
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| Keywords | 低酸素 / PAI-I / 脂肪細胞分化 / PHD / ホモfloxマウス / siRNA / 7xHRE人工レポーター / MTSアッセイ / 分解性検討試験 / 3T3-L1 / レトロウイルス / 低酸素シグナル / ルシフェラーゼアッセイ |
|---|
| Research Abstract |
Overexpression of PHD1,2,3 and 4 or siRNA-mediated knockdown of PHD1 and 2 did not affect 3T3-L1 adipogenesis. We successfully transferred and set up Phd1^<ff>Phd2^<ff>Phd3^<ff>, Phd2^<ff>, Phd3^<f+>and Phd2^<ff>Rosa26^<+/CreERT2>mice from Connecticut University. By using 7 x HRE artificial promoter, we screened various compounds and founded new compounds equivalent to cobalt.
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