| Project/Area Number |
21890038
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Chiba University |
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Principal Investigator |
ISHIKAWA Hiroshi Chiba University, 大学院・医学研究院, 助教 (70553973)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥1,235,000 (Direct Cost: ¥950,000、Indirect Cost: ¥285,000)
Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
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| Keywords | 子宮筋腫 / 免疫不全マウス / 移植モデル / エストロゲン / プロゲステロン / xenograft / トランスレーショナルリサーチ / 重症免疫不全マウス / 腎被膜下移植法 / 疾患モデル / アロマターゼ |
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| Research Abstract |
We established a new xenograft model for uterine leiomyoma in which leiomyoma cell pellets were grafted beneath the kidney capsule of super immunodeficient mice. The xenografts enlarged under coadministration of estrogen and progesterone in ovariectomized mice. Then we evaluated the efficacy of RU486, which is a progesterone antagonist, for the reduction of leiomyoma xenograft size in this model. We found that RU486 significantly inhibited the growth of the xenograft even in the presence of both estrogen and progesterone. Finally, we proved that severe immunodeficient mice are suitable as hosts instead of super immunodeficient mice. Our model would be useful for the screening of potential therapeutic agents for the uterine leiomyoma.
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