The new mechanism in the development of polycystic kidney～the role of PTEN and EGF receptor in the proximal tubule～

Research Project

Project/Area Number	21890170
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Kidney internal medicine
Research Institution	The University of Tokushima
Principal Investigator	長井幸二郎 The University of Tokushima, 病院, 講師 (40542048)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000) Fiscal Year 2010: ¥1,235,000 (Direct Cost: ¥950,000、Indirect Cost: ¥285,000) Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
Keywords	PTEN / EGF受容体 / 多発性嚢胞腎
Research Abstract	The mechanism in the development of polycystic kidney is still unclear. We developed the proximal tubule specific PTEN knockout mice and found the development of polycystic kidney. We investigated the proliferation and apoptosis in the proximal tubule and the proliferation can be the main factor of the cyst formation. We also checked the cilia and the number and the length of the cilia were not different from control mice. To clarify the role of the EGF receptor in the cyst formation, we started to establish PTEN-EGF receptor double knockout mice in the proximal tubule. Now, we are choosing the effective line to develop the double knockout.