Simulation and analysis of stem cell-carcinogenesis induced by chemical carcinogens using murine embryonic stem cell

• Project/Area Number: 21890279
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Environmental pharmacy
• Research Institution: Meijo University
• Principal Investigator: OKAMOTO Yoshinori Meijo University, 薬学部, 助教 (50512323)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000); Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000); Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
• Keywords: ES細胞 / 発がん / 性ホルモン / 乳がん / 遺伝子損傷 / 幹細胞発がん / 酸化ストレス / DNA付加体

Research Abstract

Cyp1a1 and Cyp1b1 were detected in mES cell and MEF. The extent of Cyp1b1 expression was 14 times higher in MEF as compared with mES cell. 3-Methylcholanthrene (3-MC) treatment was induced Cyp1a1 and Cyp1b1 expressions in both cells in a dose-dependent manner. Especially, Cyp1a1 was induced significantly in MEF. These results suggest that hydrophobic xenobiotics tend to be concentrated in undifferentiated cells such as tissue stem cells, although further study is needed.

Research Products

• [Journal Article] Transesterification in the microbial degradation of phthalate esters. (2011)
  • Author(s): Okamoto Y., Toda C., Ueda K., Hashizume K., Kojima N.
  • Journal Title: J.Health Sci. 57 Pages: 293-299
  • NAID: 130000800091
  • Peer Reviewed
• [Journal Article] Differentmechanisms between copper and iron in catecholamines-mediated oxidative DNA damage and disruption of gene expression in vitro. (2011)
  • Author(s): Nishino Y., Ando M., Makino R., Ueda K., Okamoto Y., Kojima N.
  • Journal Title: Neurotox. Res. 20 Pages: 84-92
  • Peer Reviewed
• [Journal Article] Combined effects of manganese, iron, copper, and dopamine on oxidative DNA damage. (2011)
  • Author(s): Ando M., Ueda K., Okamoto Y., Kojima N.
  • Journal Title: J.Health Sci. 57 Pages: 204-209
  • NAID: 130000663122
  • Peer Reviewed
• [Journal Article] Preparation of oligoselenodiglutathiones and their suppressive effects on oxidative DNAdamage induced by catechol and copper. (2011)
  • Author(s): Ando M., Nishida H., Ueda K., Itoh K., Okamoto Y., Kojima N.
  • Journal Title: J.Health Sci. 57 Pages: 72-77
  • NAID: 130000425125
  • Peer Reviewed
• [Journal Article] Modulation of oxidative DNA damage and DNA-crosslink formation induced by cis-diammine-tetrachloro-platinum(IV) in the presence of endogenous reductants. (2011)
  • Author(s): Nakai T., Ando M., Okamoto Y., Ueda K., Kojima N.
  • Journal Title: J.Inorg. Biochem. 105 Pages: 1-5
  • NAID: 130007003176
  • Peer Reviewed
• [Journal Article] Anti-breast cancer potential of SS5020, a novel benzopyran antiestrogen. (2011)
  • Author(s): Suzuki N., Liu X., Laxmi Y.R.S., Okamoto K., Kim H.J., Zhang G., Chen J.J., Okamoto Y., Shibutani S.
  • Journal Title: Int.J.Cancer 128 Pages: 974-982
  • Peer Reviewed
• [Journal Article] Anti-breast cancer potential of SS5020, a novel benzopyran antiestrogen (2011)
  • Author(s): Yoshinori Okamoto, 他8名
  • Journal Title: Int.J.Cancer Volume: 128 Pages: 974-982
  • Peer Reviewed
• [Journal Article] Modulation of oxidative DNA damage and DNA-crosslink formation induced by cis-diammine-tetrachloro-platinum(IV) in the presence of endogenous reductants (2011)
  • Author(s): Yoshinori Okamoto, 他5名
  • Journal Title: J.Inorg.Biochem. Volume: 105 Pages: 1-5
  • NAID: 130007003176
  • Peer Reviewed
• [Journal Article] Preparation of oligoselenodiglutathiones and their suppressive effects on oxidative DNA damage induced by catechol and copper. (2011)
  • Author(s): Yoshinori Okamoto, 他5名
  • Journal Title: J.Health Sci. Volume: 57 Pages: 72-77
  • NAID: 130000425125
  • Peer Reviewed
• [Journal Article] Combined effects of manganese, iron, copper, and dopamine on oxidative DNA damage (2011)
  • Author(s): Yoshinori Okamoto, 他3名
  • Journal Title: J.Health Sci. Volume: 57 Pages: 204-209
  • NAID: 130000663122
  • Peer Reviewed
• [Journal Article] Different mechanisms between copper and iron in catecholamines-mediated oxidative DNA damage and disruption of gene expression in vitro (2011)
  • Author(s): Yoshinori Okamoto, 他3名
  • Journal Title: Neurotox.Res. Volume: 20 Pages: 84-92
  • Peer Reviewed
• [Journal Article] Transesterification in the microbial degradation of phthalate esters (2011)
  • Author(s): Yoshinori Okamoto, 他4名
  • Journal Title: J.Health Sci. Volume: (未定 in press)
  • NAID: 130000800091
  • Peer Reviewed
• [Journal Article] Equine estrogen-induced mammary tumors in rats. (2010)
  • Author(s): Okamoto Y., Liu X., Suzuki N., Okamoto K., Kim H.J., Laxmi Y.R.S., Sayama K., Shibutani S.
  • Journal Title: Toxicol. Lett. 193 Pages: 224-228
  • Peer Reviewed
• [Journal Article] Anti-breast cancer potential of SS1020, a novel antiestrogen lacking estrogenic and genotoxic actions. (2010)
  • Author(s): Laxmi Y.R.S., Liu X., Suzuki N., Kim S.Y., Okamoto K., Kim H.J., Zhang G., Chen J.J., Okamoto Y., Shibutani S.
  • Journal Title: Int.J.Cancer 127 Pages: 1718-1726
  • Peer Reviewed
• [Journal Article] Penicillamine selenotrisulfide suppressed DNA strand breaks and oxidative DNA damage in vitro. (2010)
  • Author(s): Ando M., Nishida H., Itoh K., Okamoto Y., Ueda K., Kojima N.
  • Journal Title: J.Res.Inst.Meijo Univ. 9 Pages: 27-33
  • Peer Reviewed
• [Journal Article] Production of polyselenodipenicillamines, unique selenium compounds. (2010)
  • Author(s): Nishida H., Ando M., Itoh K., Ueda K., Nishida Y., Okamoto Y., Toda C., Kojima N.
  • Journal Title: Chem. Pharm. Bull. 58 Pages: 957-960
  • NAID: 130000299793
  • Peer Reviewed
• [Journal Article] Carbonyl side-chain of catechol compounds is a key structure for the suppression of copper-associated oxidative DNA damage in vitro. (2010)
  • Author(s): Ando M., Nishida H., Nishino Y., Ohbayashi M., Ueda K., Okamoto Y., Kojima N.
  • Journal Title: Toxicol. Lett. 199 Pages: 213-217
  • Peer Reviewed
• [Journal Article] Equine estrogen-induced mammary tumors in rats. (2010)
  • Author(s): Yoshinori Okamoto, 他7名
  • Journal Title: Toxicol.Lett. Volume: 193 Pages: 224-228
  • Peer Reviewed
• [Journal Article] Anti-breast cancer potential of SS1020, a novel antiestrogen lacking estrogenic and genotoxic actions. (2010)
  • Author(s): Yoshinori Okamoto, 他9名
  • Journal Title: Int.J.Cancer Volume: 127 Pages: 1718-1726
  • Peer Reviewed
• [Journal Article] Penicillamine selenotrisulfide suppressed DNA strand breaks and oxidative DNA damage in vitro. (2010)
  • Author(s): Yoshinori Okamoto, 他5名
  • Journal Title: J.Res.Inst.Meijo Univ. Volume: 9 Pages: 27-33
  • Peer Reviewed
• [Journal Article] Production of polyselenodipenicillamines, unique selenium compounds. (2010)
  • Author(s): Yoshinori Okamoto, 他7名
  • Journal Title: Chem.Pharm.Bull. Volume: 58 Pages: 957-960
  • NAID: 130000299793
  • Peer Reviewed
• [Journal Article] Carbonyl side-chain of catechol compounds is a key structure for the suppression of copper-associated oxidative DNA damage in vitro (2010)
  • Author(s): Yoshinori Okamoto, 他6名
  • Journal Title: Toxicol.Lett. Volume: 199 Pages: 213-217
  • Peer Reviewed
• [Journal Article] Equine estrogen-induced mammary tumors in rats (2010)
  • Author(s): Yoshinori Okamoto, 他7名
  • Journal Title: Toxicol.Lett. 193 Pages: 224-228
  • Peer Reviewed
• [Journal Article] Anti-breast cancer potential of SS1020, a novel antiestrogen lacking estrogenic and genotoxic actions (2010)
  • Author(s): Yoshinori Okamoto, 他9名
  • Journal Title: Int.J.Cancer (in press)
  • Peer Reviewed
• [Presentation] セレン架橋チオール化合物の酸化的DNA損傷抑制作用と還元型チオール共存による作用変動 (2011)
  • Author(s): 岡本誉士典, 他7名
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] 生体内還元物質共存下におけるPt(IV)化合物の酸化的DNA損傷と配意子の影響 (2011)
  • Author(s): 岡本誉士典, 他5名
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] Fe(III)-/Cu(II)-カテコール化合物介在性酸化的DNA損傷に対するMn(II)のSOD様作用・自動酸化促進による増強効果 (2011)
  • Author(s): 岡本誉士典,, 他5名
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] 植物由来化合物によるエストロゲン受容体転写調節における構造的特徴 (2011)
  • Author(s): 岡本誉士典, 他5名
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-30
• [Presentation] マンガンのSOD様作用・自動酸化促進による鉄-/銅-カテコール誘導酸化的DNA損傷の増強 (2011)
  • Author(s): 岡本誉士典, 他5名
  • Organizer: 第81回日本衛生学会学術総会
  • Place of Presentation: 静岡
  • Year and Date: 2011-03-26
• [Presentation] マンガンのSOD様作用・自動酸化促進による鉄-/銅-カテコール誘導酸化的DNA損傷の増強 (2011)
  • Author(s): 岡本誉士典, 他5名
  • Organizer: 第81回日本衛生学会学術総会
  • Place of Presentation: 東京
  • Year and Date: 2011-03-26
• [Presentation] マウス胚性幹細胞を用いた神経細胞分化と化学物質による影響評価 (2011)
  • Author(s): 岡本誉士典, 他1名
  • Organizer: 名城大学学術フロンティア推進事業第3回若手研究者シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2011-01-08
• [Presentation] マウス胚性幹細胞を用いた神経細胞分化と化学物質による影響評価 (2011)
  • Author(s): 岡本誉士典, 他1名
  • Organizer: 名城大学学術フロンティア推進事業 第3回若手研究者シンポジウム
  • Place of Presentation: 名古屋
  • Year and Date: 2011-01-08
• [Presentation] 植物由来化合物によるエストロゲン受容体転写調節と成分総和による作用緩和 (2010)
  • Author(s): 岡本誉士典, 他7名
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会
  • Place of Presentation: 静岡
  • Year and Date: 2010-11-28
• [Presentation] 新しい抗エストロゲン化合物:遺伝毒性および女性ホルモン作用の無い乳がん治療薬の開発 (2010)
  • Author(s): 岡本誉士典, 他4名
  • Organizer: 日本環境変異原学会第39回大会
  • Place of Presentation: つくば
  • Year and Date: 2010-11-28
• [Presentation] 植物由来化合物によるエストロゲン受容体転写調節と成分総和による作用緩和 (2010)
  • Author(s): 岡本誉士典, 他7名
  • Organizer: 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2010
  • Place of Presentation: 静岡
  • Year and Date: 2010-11-28
• [Presentation] 生体内チオール化合物のセレン架橋生成物が示す遺伝子損傷抑制作用 (2010)
  • Author(s): 岡本誉士典, 他7名
  • Organizer: フォーラム2010衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Presentation] エストロゲン受容体転写に対するブラジル産プロポリス各種成分の多彩な正負作用と成分総和による作用緩和 (2010)
  • Author(s): 岡本誉士典, 他6名
  • Organizer: フォーラム2010衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Presentation] カテコールアミンによる遺伝子損傷におけるロイコアミノクロム生成の重要性 (2010)
  • Author(s): 岡本誉士典, 他4名
  • Organizer: フォーラム2010衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Presentation] エストロゲン受容体転写に対するブラジル産プロポリス各種成分の多彩な正負作用と成分総和による作用緩和 (2010)
  • Author(s): 岡本誉士典, 他5名
  • Organizer: フォーラム2010 衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Presentation] カテコールアミンによる遺伝子損傷におけるロイコアミノクロム生成の重要性 (2010)
  • Author(s): 岡本誉士典, 他4名
  • Organizer: フォーラム2010 衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-10
• [Presentation] 生体内チオール化合物のセレン架橋生成物が示す遺伝子損傷抑制作用 (2010)
  • Author(s): 岡本誉士典, 他7名
  • Organizer: フォーラム2010 衛生薬学・環境トキシコロジー
  • Place of Presentation: 東京
  • Year and Date: 2010-09-09
• [Presentation] ポリセレノジペニシラミンの生成機構とCu(II)-カテコール依存的DNA損傷抑制効果 (2010)
  • Author(s): 岡本誉士典, 他7名
  • Organizer: 第56回日本薬学会東海支部総会・大会
  • Place of Presentation: 岐阜
  • Year and Date: 2010-07-03
• [Presentation] Metal-mediated oxidative DNA damage induced by endogenous catecholamines and its suppressive effect on gene expression (2010)
  • Author(s): 岡本誉士典, 他4名
  • Organizer: 第20回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 徳島
  • Year and Date: 2010-06-26
• [Presentation] Metal-mediated oxidative DNA damage induced by endogenous catecholamines and its suppressive effect on gene expression (2010)
  • Author(s): 岡本誉士典, 他4名
  • Organizer: 第20回金属の関与する生体関連反応シンポジウムSRM2010
  • Place of Presentation: 徳島
  • Year and Date: 2010-06-26
• [Presentation] カテコールアミン酸化中間体および生体内金属による酸化的DNA損傷と神経変性における役割 (2010)
  • Author(s): 岡本誉士典, 他4名
  • Organizer: 第63回日本酸化ストレス学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2010-06-24
• [Presentation] 生体内還元物質によって変動するPt(IV)化合物の酸化的DNA損傷および架橋形成作用 (2010)
  • Author(s): 岡本誉士典, 他5名
  • Organizer: 第37回日本トキシコロジー学会学術年会
  • Place of Presentation: 宜野湾
  • Year and Date: 2010-06-17
• [Presentation] 食品由来成分によるエストロゲン受容体転写発現への影響 (2010)
  • Author(s): 岡本誉士典, 他6名
  • Organizer: 日本食品化学学会第16回総会・学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2010-06-10
• [Presentation] Production of polyselenodipenicillamines, unique selenium compounds. (2010)
  • Author(s): 岡本誉士典, 他5名
  • Organizer: 9th International Symposium on Selenium in Biology and Medicine
  • Place of Presentation: 京都
  • Year and Date: 2010-06-01
• [Presentation] Production of polyselenodipenicillamines, unique selenium compounds (2010)
  • Author(s): Yoshinori Okamoto, 他5名
  • Organizer: 9th International Symposium on Selenium in Biology and Medicine
  • Place of Presentation: 京都
  • Year and Date: 2010-06-01
• [Presentation] Novel antiestrogens, SS1020 and SS5020, for breast cancer therapy and prevention. (2010)
  • Author(s): 岡本誉士典, 他5名
  • Organizer: AACR 101st Annual Meeting
  • Place of Presentation: 米国ワシントンD.C.
  • Year and Date: 2010-04-20
• [Presentation] DNA oxidation induced by platinum(IV) compound in the presence of endogenous reductants. (2010)
  • Author(s): 岡本誉士典, 他5名
  • Organizer: AACR 101st Annual Meeting
  • Place of Presentation: 米国ワシントンD.C.
  • Year and Date: 2010-04-20
• [Presentation] DNA oxidation induced by platinum(IV) compound in the presence of endogenous reductants (2010)
  • Author(s): Yoshinori Okamoto, 他5名
  • Organizer: AACR 101st Annual Meeting 2010
  • Place of Presentation: ワシントンDC、アメリカ
  • Year and Date: 2010-04-20
• [Presentation] Novel antiestrogens, SS1020 and SS5020, for breast cancer therapy and prevention (2010)
  • Author(s): Yoshinori Okamoto, 他5名
  • Organizer: AACR 101st Annual Meeting 2010
  • Place of Presentation: ワシントンDC、アメリカ
  • Year and Date: 2010-04-20
• [Remarks] ホームページ等
  • URL: http://www-yaku.meijo-u.ac.jp/Research/Laboratory/hygie_chem/index.html
• [Remarks]
  • URL: http://www-yaku.meijo-u.ac.jp/Research/Laboratory/hygie_chem/publications.html