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Molecular interactome on the regulation of lanthanide-dependent methylotrophy

Research Project

Project/Area Number 21H02105
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38020:Applied microbiology-related
Research InstitutionOkayama University

Principal Investigator

Tani Akio  岡山大学, 資源植物科学研究所, 准教授 (00335621)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2023: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2022: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2021: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
KeywordsMethanol / Methylobacterium / Lanthanide / lanthanide / Methylotroph / Methanol dehydrogenase / ランタノイド / メタノール代謝 / methylotroph / Methylotrophy / Interactome
Outline of Research at the Start

Methylobacterium属細菌はメタノールの資化にカルシウム依存MxaFとランタノイド依存のXoxFの二種類のメタノール脱水素酵素(MDH)を持つ。これらはランタノイドの有無により制御されている。この制御機構は複雑であり、遺伝的解析により必要な因子は分かりつつあるが、制御機構におけるそれらの位置関係は分かっていない。本研究ではこれら因子間の相互作用の有無を解析することによりランタノイドに応答する制御分子機構を解明する。

Outline of Final Research Achievements

Methylobacterium species are methylotrophic plant-symbiotic bacteria residing in the phyllosphere. They usually have Ca-dependent MxaF and Ln-dependent XoxF, both of which are methanol dehydrogenase. The expression of these genes is switched by the presence of Ln. In this study we investigated the molecular mechanism of the Ln switch, genetic and biochemistry of the siderophore and lanmodulin, genetic and phenotypic analysis on methanol-chemotaxis that is also dependent on Ln, and compartive genomics across all species in the genus to elucidate the core genomes of Methylobacterium.

Academic Significance and Societal Importance of the Research Achievements

Lnは生物学的には重要でないと考えられてきたが、メタノール資化性のグラム陰性細菌はほぼ全てLn依存のMDHを持っていることが分かってきた。Methylobacteriumは植物葉上の細菌であるためLn依存酵素だけでなくCa依存酵素を持っていると考えられる。これまで必須と考えられてきたCa依存MxaFは、ラボ環境のような特別な条件でしか必要ではなく、自然界ではLn依存XoxFが主に使われている。本属細菌ではそれが切りかわる現象があり、その分子メカニズムに迫ることを通じて、本研究では生物における新しい金属の機能と役割を明らかにしつつある。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (26 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (2 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (19 results) (of which Int'l Joint Research: 5 results,  Invited: 1 results)

  • [Int'l Joint Research] Inst Res Genet Eng Mol Biol(アルゼンチン)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Mugla university(トルコ)

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Metabolism-linked methylotaxis sensors responsible for plant colonization in Methylobacterium aquaticum strain 22A2023

    • Author(s)
      Tani Akio、Masuda Sachiko、Fujitani Yoshiko、Iga Toshiki、Haruna Yuuki、Kikuchi Shiho、Shuaile Wang、Lv Haoxin、Katayama Shiori、Yurimoto Hiroya、Sakai Yasuyoshi、Kato Junichi
    • Journal Title

      Frontiers in Microbiology

      Volume: 14 Pages: 1258452-1258452

    • DOI

      10.3389/fmicb.2023.1258452

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Periplasmic Lanthanide Mediator, Lanmodulin, in Methylobacterium aquaticum Strain 22A2022

    • Author(s)
      Fujitani Yoshiko、Shibata Takeshi、Tani Akio
    • Journal Title

      Frontiers in Microbiology

      Volume: 13 Pages: 921636-921636

    • DOI

      10.3389/fmicb.2022.921636

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Siderophore for Lanthanide and Iron Uptake for Methylotrophy and Plant Growth Promotion in Methylobacterium aquaticum Strain 22A2022

    • Author(s)
      Juma Patrick Otieno、Fujitani Yoshiko、Alessa Ola、Oyama Tokitaka、Yurimoto Hiroya、Sakai Yasuyoshi、Tani Akio
    • Journal Title

      Frontiers in Microbiology

      Volume: 13 Pages: 921635-921635

    • DOI

      10.3389/fmicb.2022.921635

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comprehensive Comparative Genomics and Phenotyping of Methylobacterium Species2021

    • Author(s)
      Ola Alessa, Yoshitoshi Ogura, Yoshiko Fujitani, Hideto Takami, Tetsuya Hayashi, Nurettin Sahin, Akio Tani
    • Journal Title

      Frontiers in Microbiology

      Volume: 12 Pages: 740610-740610

    • DOI

      10.3389/fmicb.2021.740610

    • NAID

      120007170513

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Discovery of lanthanide-dependent methylotrophy and screening methods for lanthanide-dependent methylotrophs2021

    • Author(s)
      Tani Akio、Mitsui Ryoji、Nakagawa Tomoyuki
    • Journal Title

      Methods in Enzymology

      Volume: 650 Pages: 1-18

    • DOI

      10.1016/bs.mie.2021.01.031

    • ISBN
      9780128238561
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] 葉面細菌Methylobacteriumのバクテリオクロロフィル生産と遺伝子発現2024

    • Author(s)
      井口 博之、宮部 業、吉田 朱里、谷 明生
    • Organizer
      日本農芸化学会2024年度東京大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Methylobacterium aquaticum 22AにおけるLanmodulinの機能解析2023

    • Author(s)
      藤谷良子、柴田猛、谷 明生
    • Organizer
      農芸化学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Signal crosstalk between Methylobacterium sp. 2A and Arabidopsis thaliana2023

    • Author(s)
      Cecilia E.M. Grossi, AkioTani, Rita M. Ulloa
    • Organizer
      PMRN 2013
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Metabolism-linked methanol chemotaxis in Methylobacterium aquaticum strain 22A.2022

    • Author(s)
      Tani A., Iga T., Haruna Y., Kikuchi S., Masuda S., Fujitani Y., Kato J.
    • Organizer
      International society of microbial ecology 18
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ランタノイド依存型C1細菌の植物共生と生育促進技術への応用2022

    • Author(s)
      久田健司、野村颯人、根本侑知、水野洸介、三井亮司、谷明生、井口博之、清水将文、島田昌也、中川智行
    • Organizer
      2022 日本生物工学会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 植物共生メチロトローフ細菌 Methylorubrum extorquens AM1 のメタノールデヒドロゲナーゼアイソザイムの発現制御に関わる二成分制御系の解析2022

    • Author(s)
      高橋莉史,矢野嵩典,谷明生,中川智行,三井亮司
    • Organizer
      日本生物工学会西日本支部大会2022
    • Related Report
      2022 Annual Research Report
  • [Presentation] Methylobacterium sp. OR01株におけるメタノール走化性に関わるMCPタンパク質の同定2022

    • Author(s)
      加地奏絵、川端和弥、片山志織、谷明生、由里本博也、阪井康能
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 植物共生メチロトローフ細菌Methylorubrum extorquens AM1のランタノイドに応答したメタノールデヒドロゲナーゼXoxF1の発現調節機構の解析2022

    • Author(s)
      高橋 莉史、矢野 嵩典、中川 智行、谷 明生、三井 亮司
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] グラム陽性メチロトローフ細菌Arthrobacter sp. YM1のランタノイド依存的メタノール生育に関する研究2022

    • Author(s)
      森田 翔太、八木 茜、矢野 嵩典、谷 明生、中川 智行、阿野 嘉孝、三井 亮司
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Methylobacterium aquaticum 22A株におけるメタノール走化性の分子機構の解明2022

    • Author(s)
      菊池 志保、藤谷 良子、加藤 純一、谷 明生
    • Organizer
      日本農芸化学会2022年度大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Functional analysis of siderophore in Methylobacterium aquaticum 22A.2021

    • Author(s)
      Patrick Juma, Ola Alessa, Yoshiko Fujitani, Tokitaka Oyama, Hiroya Yurimoto, Yasuyoshi Sakai, Akio Tani
    • Organizer
      Phyllosphere Fortnight 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparative genomics and phenotyping approach on Methylobacterium species.2021

    • Author(s)
      Alessa O., Ogura Y., Fujitani Y., Hayashi T., Sahin N., Tani A.
    • Organizer
      Phyllosphere Fortnight 2021
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物表層共生細菌の生態と応用2021

    • Author(s)
      谷 明生
    • Organizer
      第2回生物刺激制御研究会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Comparative genomic analysis of the pink pigmented facultative methylotrophs (PPFMs)2021

    • Author(s)
      Ola Alessa, Yoshitoshi Ogura, Yoshiko Fujitani, Hideto Takami, Tetsuya Hayashi, Akio Tani
    • Organizer
      Galaxy online meeting
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] グラム陽性メチロトローフ細菌Arthrobacter sp. YM1の新規ランタノイド依存型メタノールデヒドロゲナーゼの精製と諸性質2021

    • Author(s)
      森田翔太, 任家宜, 谷明生, 中川智行, 阿野嘉孝, 矢野嵩典, 三井亮司
    • Organizer
      日本農芸化学会西日本・中四国・関西支部合同大会(第60回講演会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] Genomic insights into the PPFMs phylogeny and unique features2021

    • Author(s)
      Ola Alessa, Yoshitoshi Ogura, Yoshiko Fujitani, Hideto Takami, Tetsuya Hayashi, Nurettin Sahin, Akio Tani
    • Organizer
      日本農芸化学会西日本・中四国・関西支部合同大会(第60回講演会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] メチロバクテリウム属細菌におけるメタノール走化性の分子メカニズムの解明2021

    • Author(s)
      菊池志保, 藤谷良子,加藤純一, 谷明生
    • Organizer
      日本農芸化学会西日本・中四国・関西支部合同大会(第60回講演会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] 低栄養下におけるMethylorubrum属細菌のランタノイドによる生育促進応答に関する研究2021

    • Author(s)
      根本侑知,水野洸介,原田雄斗,岩本悟志,谷明生,三井亮司,島田昌也,早川享志,中川智行
    • Organizer
      日本生物工学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] メチロバクテリウムがシロイヌナズナの形質に及ぼす影響とその共生作用2021

    • Author(s)
      井内敦子,飯野隆夫,朝比奈雅志,谷明生,成川(奈良)恵,市橋泰範,大熊盛也,安部洋,小林正智
    • Organizer
      植物化学調節学会
    • Related Report
      2021 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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