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Speciation via reinforcement as revealed by evolutionary developmental biology and comparative genomics

Research Project

Project/Area Number 21H02566
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 45040:Ecology and environment-related
Research InstitutionKobe University

Principal Investigator

Takami Yasuoki  神戸大学, 人間発達環境学研究科, 教授 (60432358)

Co-Investigator(Kenkyū-buntansha) 小笠原 道生  千葉大学, 大学院理学研究院, 准教授 (00343088)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
Fiscal Year 2023: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2022: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2021: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Keywordsエボデボ / 交尾器 / 種分化 / 生殖隔離 / 交尾器形態 / 形質置換
Outline of Research at the Start

本研究は,交尾器形態の違いが機械的生殖隔離をもたらすオオオサムシ類について,種分化過程における交尾器形態とゲノムの分化機構と,形態の遺伝発生学的基盤を明らかにすることを目的とする.種間交雑に伴う形態とゲノムの分化機構を解明するために,分布を接する近縁種を対象に,行動実験によって交尾器形態の形質置換をもたらした自然淘汰を定量するとともに,集団ゲノミクス解析 により,自然淘汰と遺伝子流動の影響下にあるゲノムの分化動態を解明する.また,マイクロCTとin situ hybridizationを用いた形態形成・遺伝子発現解析により,交尾器形態の発生過程と,形態の種間差に関わる遺伝的背景を解明する.

Outline of Final Research Achievements

We detected reinforcing selection possibly responsible for reproductive character displacement between Carabus maiyasanus and C. iwawakianus based on interspecific mating experiments. For analyzing genomic differentiation concerning such a morphological differentiation, we sequenced genomes of a total of 25 individuals throughout the distributional ranges.
We analyzed morphogenetic processes of extremely enlarged male and female genitalia of C. uenoi by micro-CT analyses. In addition, we examined constraints on genital exaggeration in C. uenoi by morphometric and comparative analyses. To reveal the genes responsible for such morphological differentiation and enlargement in the genitalia, we developed a protocol for in situ hybridization that is optimized for ground beetles.

Academic Significance and Societal Importance of the Research Achievements

本研究は,生物多様性を創出する根源的過程である種分化に関わる,交尾器形態の分化,多様化の進化機構と,その背景にある遺伝発生学的基盤を明らかにしようとするものである.材料であるオサムシは昆虫の中でも進化生態学的研究が進んでおり,一般にも良く知られている生物であるため,このような対象についての新たな知見は,社会全体の生物に対する知的好奇心を高め,自然環境に対する認識や理解を涵養することにつながると期待される.

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (9 results)

All 2024 2023 2022 2021

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results) Presentation (5 results) (of which Invited: 1 results)

  • [Journal Article] Functional, genetic, and structural constraints on the exaggeration and diversification of male genital morphology in <i>Ohomopterus</i> ground beetles2023

    • Author(s)
      Terada Karen、Takahashi Sougo、Takami Yasuoki
    • Journal Title

      Entomological Science

      Volume: 26 Issue: 1

    • DOI

      10.1111/ens.12538

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relationships between reproductive character displacement in genital morphology and the population-level cost of interspecific mating: implications for the Templeton effect2023

    • Author(s)
      Nishimura Taira、Terada Karen、Xia Tian、Takami Yasuoki
    • Journal Title

      Biological Journal of the Linnean Society

      Volume: 138 Issue: 1 Pages: 14-26

    • DOI

      10.1093/biolinnean/blac126

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
  • [Journal Article] Reproductive isolation via divergent genital morphology due to cascade reinforcement in <i>Ohomopterus</i> ground beetles2022

    • Author(s)
      Xia Tian、Nishimura Taira、Nagata Nobuaki、Kubota Kohei、Sota Teiji、Takami Yasuoki
    • Journal Title

      Journal of Evolutionary Biology

      Volume: 36 Issue: 1 Pages: 169-182

    • DOI

      10.1111/jeb.14116

    • Related Report
      2023 Annual Research Report 2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Reproductive Character Displacement in Genital Morphology in <i>Ohomopterus</i> Ground Beetles2022

    • Author(s)
      Nishimura Taira、Nagata Nobuaki、Terada Karen、Xia Tian、Kubota Kohei、Sota Teiji、Takami Yasuoki
    • Journal Title

      The American Naturalist

      Volume: 199 Issue: 3 Pages: E76-E90

    • DOI

      10.1086/717864

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] 交尾器形態の生殖的形質置換:壊れにくく異種を識別しやすい柔らかい雄交尾器の進化2024

    • Author(s)
      西村太良, 寺田夏蓮, 井上真理, 高見泰興
    • Organizer
      日本生態学会第71回全国大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] オオオサムシ亜属における交尾器の巨大化に関わる進化発生学的要因2024

    • Author(s)
      古本知奈美, 寺田夏蓮, 高見泰興
    • Organizer
      日本生態学会第71回全国大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 生殖的形質置換に伴う雄交尾器の柔らかさの進化2022

    • Author(s)
      西村太良, 高見泰興, 井上真理
    • Organizer
      日本生態学会第69回全国大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 雄交尾器の巨大化に伴うボディーパーツ間の相関構造の変化2022

    • Author(s)
      寺田夏蓮, 高橋颯吾, 高見泰興
    • Organizer
      日本生態学会第69回全国大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 性的対立をもたらす交尾器形態が野生個体群動態と分布域形成におよぼすインパクト2021

    • Author(s)
      高見泰興
    • Organizer
      日本進化学会第23回大会シンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited

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Published: 2021-04-28   Modified: 2025-01-30  

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