Spatiotemporal analysis of aqueous reaction field of plasma-generated free radicals
Project/Area Number |
21H04451
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Review Section |
Medium-sized Section 14:Plasma science and related fields
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Research Institution | Nagoya University |
Principal Investigator |
石川 健治 名古屋大学, 低温プラズマ科学研究センター, 教授 (60417384)
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Co-Investigator(Kenkyū-buntansha) |
田中 宏昌 名古屋大学, 低温プラズマ科学研究センター, 教授 (00508129)
古閑 一憲 九州大学, システム情報科学研究院, 教授 (90315127)
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Project Period (FY) |
2021-04-05 – 2024-03-31
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Project Status |
Completed (Fiscal Year 2023)
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Budget Amount *help |
¥43,030,000 (Direct Cost: ¥33,100,000、Indirect Cost: ¥9,930,000)
Fiscal Year 2023: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2022: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2021: ¥23,790,000 (Direct Cost: ¥18,300,000、Indirect Cost: ¥5,490,000)
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Keywords | プラズマ / 液相ラジカル / ラジカル / 電子スピン共鳴 |
Outline of Research at the Start |
放電プラズマによる抗腫瘍作用などのプラズマと生体との相互作用に関する新奇現象においては,プラズマが生成するラジカルが起点となり,逐次連鎖反応により次々と別のラジカルが生じ,それらの作用で細胞内代謝に変動が生じている.本研究は「プラズマ生成ラジカルが誘起する逐次連鎖反応はどのように進んでいるか?」に焦点を絞い,プラズマ生成ラジカルを起点とする逐次連鎖反応で生成する複数のラジカルをμs・mmの時空間分解能で検出・解析する.得られた結果を総括し,プラズマが誘起する液相に生じる非平衡反応場内のラジカルの反応と,逐次連鎖的に進行するラジカル誘起反応について体系的にまとめる.
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Outline of Annual Research Achievements |
本研究の目的は,新興プラズマバイオ大気圧低温プラズマの生体作用の学理の探究であり,核心をなす学術的な「問い」は「プラズマによって細胞内代謝に変動が及ぼされる要因とその作用機構」とは何かである.このようなプラズマが新奇に及ぼす作用の根幹は,液相中へのプラズマ起因のラジカル生成にある.しかしながら,生体近傍に特異に高い濃度で生成するラジカル群が,細胞膜上の受容体に作用して,その結果として細胞内代謝変動に発展する過程は未知であり,それらを知るためには,プラズマが生成する液相ラジカル解明と,その逐次連鎖的に生じる反応の解析が必要がある. プラズマ中では高速電子が分子と衝突してフリーラジカルを生成する.このラジカルが起点となり,逐次連鎖反応により次々と別のラジカルが生じ,それらの作用で細胞内化学反応(代謝)に変動が生じる. 本研究では「プラズマ生成ラジカルが誘起する逐次連鎖反応はどのように進んでいるか?」に焦点を絞り,プラズマが溶液内に生成したラジカルを起点とする逐次連鎖反応で生じる複数の『プラズマ誘起活性種』の検出・解析してきた.液相ラジカルの反応は有機分子の合成・修飾・分解,Ⅰ)官能基の置換,Ⅱ)C3(炭素原子が三つの化合物)からC4, C5への付加,Ⅲ)C2,C1への分解,に大別される.これらの液相ラジカルの反応は,非熱的に連鎖してラジカル発生する特徴を有する.非平衡に進む化学反応が故に,反応その場で定量的に解析される計測科学が重要であることを実証してきた.液相ラジカルの反応を,溶液内の物資の拡散や流れを考慮して,時間と空間を区分し,低温大気圧プラズマでは,10の15乗程度の高いプラズマ密度の環境で発生する有機分子について液相ラジカルの反応の理解を通して進められた.
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Research Progress Status |
令和5年度が最終年度であるため、記入しない。
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Strategy for Future Research Activity |
令和5年度が最終年度であるため、記入しない。
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Report
(4 results)
Research Products
(126 results)
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[Journal Article] Cancer-specific cytotoxicity of Ringer's acetate solution irradiated by cold atmospheric pressure plasma2023
Author(s)
Miron, Camelia; Ishikawa, Kenji; Kashiwagura, Satoshi; Suda, Yuki; Tanaka, Hiromasa; Nakamura, Kae; Kajiyama, Hiroaki; Toyokuni, Shinya; Mizuno, Masaaki; Hori, Masaru
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Journal Title
Free Radic. Res.
Volume: 57
Issue: 2
Pages: 91-104
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Generation and measurement of low-temperature plasma for cancer therapy: a historical review2023
Author(s)
Ishikawa, Kenji; Takeda, Keigo; Yoshimura, Shinji; Kondo, Takashi; Tanaka, Hiromasa; Toyokuni, Shinya; Nakamura, Kae; Kajiyama, Hiroaki; Mizuno, Masaaki; Hori, Masaru
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Journal Title
Free Radic. Res.
Volume: 57
Issue: 3
Pages: 239-270
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Elaborate cooperation of poly(rC)-binding proteins 1/2 and glutathione in ferroptosis induced by plasma-activated Ringer's lactate2023
Author(s)
Jiang, Li; Zheng, Hao; Ishida, Moe; Lyu, Qinying; Akatsuka, Shinya; Motooka, Yashiro; Sato, Kotaro; Sekido, Yoshitaka; Nakamura, Kae; Tanaka, Hiromasa; Ishikawa, Kenji; Kajiyama, Hiroaki; Mizuno, Masaaki; Hori, Masaru; Toyokuni, Shinya
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Journal Title
Free Radic. Biol. Med.
Volume: 214
Pages: 28-41
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Leukocyte depletion and size-based enrichment of circulating tumor cells using a pressure-sensing microfiltration device.2022
Author(s)
Onoshima D, *Hase T, Kihara N, Kuboyama D, Tanaka H, Ozawa N, Yukawa H, Sato M, Ishikawa K, Hasegawa Y, Ishii M, Hori M, Baba Y.
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Journal Title
ACS Measurement Science Au
Volume: 3
Issue: 2
Pages: 113-119
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Chemically Active Compounds Formed in Low-temperature Plasma Treated Liquids for Cancer Treatment2023
Author(s)
Camelia Miron, Hiromasa Tanaka, Taishi Yamakawa, Du Lyin, Hiroki Kondo, Hiroshi Hashizume, Takashi Kondo, Kenji Ishikawa, Shinya Toyokuni, Masaaki Mizuno, and Masaru Hori
Organizer
Global Plasma Forum in Aomori
Related Report
Int'l Joint Research
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[Presentation] On the mechanism of high-speed SiO2 etching using hydrogen fluoride-contained plasmas at cryogenic temperature2023
Author(s)
Shih-Nan Hsiao, Makoto Sekine, Nikolay Britun, Michael Kin-Ting Mo, Yusuke Imai, Takayoshi Tsusumi, Kenji Ishikawa, Yuki Iijima, Masahiko Yokoi, Ryutaro Suda, Yoshihide Kihara and Masaru Hori
Organizer
Global Plasma Forum in Aomori
Related Report
Int'l Joint Research
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[Presentation] Etching Selectivities of SiO2 and SiN Against a-C Films Using CF4/H2 with a Pseudo-Wet Plasma Etching Mechanism2023
Author(s)
Yusuke Imai, S. Hsiao, M. Sekine, T. Tsutsumi, K. Ishikawa, M. Iwata, M. Tamura, Y. Iijima, T. Gohira, K. Matsushima, Y. Ohya, M. Hori,
Organizer
AVS 69th International Symposium and Exhibition (AVS 69), Portland, USA
Related Report
Int'l Joint Research
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[Presentation] Cold plasma treatment during cultivation affects flesh firmness of strawberry fruits2023
Author(s)
Hiroshi Hashizume, Kohei Nakano, Kaoru Sanda, Akiko Abe, Hiroko Mizuno, Manasikan Thammawong, Camelia Miron, Nikolay Britun, Teruaki Shimazu, Hiromasa Tanaka, and Masaru Hori
Organizer
ISPlasma2023 / IC-PLANTS2023
Related Report
Int'l Joint Research
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[Presentation] Control of generating white core in grains of brewer’s rice cultivar with direct plasma irradiation to spikelet after flowering2023
Author(s)
Hiroshi Hashizume, Hidemi Kitano, Hiroko Mizuno, Akiko Abe, Kaoru Sanda, Shih-Nan Hsiao, Genki Yuasa, Satoe Tohno, Hiromasa Tanaka, Kenji Ishikawa, Shogo Matsumoto, Hitoshi Sakakibara, Yuji Hirosue , Masayoshi Maeshima, Masaaki Mizuno, and Masaru Hori
Organizer
ISPlasma2023 / IC-PLANTS2023
Related Report
Int'l Joint Research
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[Presentation] Effectiveness of Plasma Treatment for Various Rice Cultivation2022
Author(s)
Hiroshi Hashizume, Hidemi Kitano, Hiroko Mizuno, Akiko Abe, Kaoru Sanda, Genki Yuasa, Satoe Tohno, Hiromasa Tanaka, Kenji Ishikawa, Shogo Matsumoto, Hitoshi Sakakibara, Susumu Nikawa, Masayoshi Maeshima, Masaaki Mizuno, Masaru Hori
Organizer
9th International Conference on Plasma Medicine (ICPM9)
Related Report
Int'l Joint Research
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[Presentation] Comprehensive analysis of gene expression in PAL-treated glioblastoma cells2022
Author(s)
Hiromasa Tanaka, Masaaki Mizuno, Ayako Tanaka, Yuki Shibata, Kenji Ishikawa, Hiroki Kondo, Hiroshi Hashizume, Camelia Miron, Yasumasa Okazaki, Shinya Toyokuni, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaru Hori
Organizer
11th International Conference on Reactive Plasmas/2022 Gaseous Electronics Conference (ICRP-11/GEC2022)
Related Report
Int'l Joint Research
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[Presentation] Various approaches of cold plasma treatment to brewer’s rice plant for improvement of grain quality2022
Author(s)
Hiroshi Hashizume, Hidemi Kitano, Hiroko Mizuno, Akiko Abe, Kaoru Sanda, Genki Yuasa, Satoe Tohno, Shih-Nan Hsiao, Hiromasa Tanaka, Kenji Ishikawa, Shogo Matsumoto, Hitoshi Sakakibara, Yoji Hirosue, Masayoshi Maeshima, Masaaki Mizuno, Masaru Hori
Organizer
11th International Conference on Reactive Plasmas/2022 Gaseous Electronics Conference (ICRP-11/GEC2022)
Related Report
Int'l Joint Research
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[Presentation] Analysis of Cellular Respiration in Plasma-activated Solutions-treated Cancer Cells2021
Author(s)
Hiromasa Tanaka, Shogo Maeda, Masaaki Mizuno, Kenji Ishikawa, Kae Nakamura, Hiroaki Kajiyama, Yasumasa Okazaki, Shinaya Toyokuni, M. Ito, K. Ohno, Fumitaka Kikkawa, and Masaru Hori
Organizer
8th International Conference on Plasma Medicine (ICPM8)
Related Report
Int'l Joint Research
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[Presentation] Dynamical Changes in Free-radical Signals of Seeds during Water Imbibition and Seed Germination: Analysis of Plasma-Irradiation Effects Using an Electron Spin Resonance (ESR) Technique2021
Author(s)
Kenji Ishikawa, Ryo Arita, Takamasa Okumura, Pankaj Attri, Kazunori Koga, Ryoya Sato, Hayate Tanaka, Masaya Hiromatsu, Kayo Matsuo, Daisuke Yamashita, Kunihiro Kamataki, Naho Itagaki, Masaru Hori, and Masaharu Shiratani
Organizer
8th International Conference on Plasma Medicine (ICPM8)
Related Report
Int'l Joint Research
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[Presentation] Efficacy for Rice Plant Growth with Plasma Irradiation to Seeds2021
Author(s)
Hiroshi Hashizume, Hidemi Kitano, Hiroko Mizuno, Akiko Abe, Genki Yuasa, Satoe Tohno, Hiromasa Tanaka, Kenji Ishikawa, Shogo Matsumoto, Hitoshi Sakakibara, Susumu Nikawa, Masayoshi Maeshima, Masaaki Mizuno, and Masaru Hori
Organizer
8th International Conference on Plasma Medicine (ICPM8)
Related Report
Int'l Joint Research
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[Presentation] Production of plasma-activated Ringer's lactate solution with regulated surrounding atmosphere2021
Author(s)
Daiki Ito, Naoyuki Iwata, Kenji Ishikawa, Hiroshi Hashizume, Kae Nakamura, Camelia Miron, Hiromasa Tanaka, Hiroaki Kajiyama, Shinya Toyokuni, Masaaki Mizuno, and Masaru Hori
Organizer
31st Material Research Society in Japan (MRS-J)
Related Report
Int'l Joint Research
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[Presentation] Anti-tumor effect of plasma-activated solution produced by the flowing system2021
Author(s)
Satoshi Kashiwagura, Naoyuki Iwata, Kenji Ishikawa, Hiroshi Hashizume, Camelia Miron, Kae Nakamura, Hiromasa Tanaka, Hiroaki Kajiyama, Shinya Toyokuni, Masaaki Mizuno, and Masaru Hori
Organizer
31st Material Research Society in Japan (MRS-J)
Related Report
Int'l Joint Research
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[Presentation] Effects of cold plasma treatment with rice seedlings in a paddy on yield and grain quality for different cultivars2021
Author(s)
Hiroshi Hashizume, Hidemi Kitano, Hiroko Mizuno, Akiko Abe, Kaoru Sanda, Genki Yuasa, Satoe Tohno, Hiromasa Tanaka, Kenji Ishikawa, Shogo Matsumoto, Hitoshi Sakakibara, Susumu Nikawa, Masayoshi Maeshima, Masaaki Mizuno, and Masaru Hori
Organizer
31st Material Research Society in Japan (MRS-J)
Related Report
Int'l Joint Research
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[Presentation] Cellular Respiration System Affected by Low-temperature Plasma2021
Author(s)
Hiromasa Tanaka, Shogo Maeda, Shogo Matsumura, Masaaki Mizuno, Kenji Ishikawa, Masafumi Ito, Hiroshi Hashizume, Mikako Ito, Kinji Ohno, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Yasumasa Okazaki, Shinya Toyokuni, and Masaru Hori
Organizer
31st Material Research Society in Japan (MRS-J)
Related Report
Int'l Joint Research
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