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Study of technological basis for application of microbial monitoring in subsurface resource exploration

Research Project

Project/Area Number 21K04961
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 31020:Earth resource engineering, Energy sciences-related
Research InstitutionThe University of Tokyo

Principal Investigator

Kobayashi Hajime  東京大学, 大学院工学系研究科(工学部), 准教授 (50549269)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2022: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords資源開発 / 石油 / 貯留層 / モニタリング / トレーサー / 環境微生物 / 油ガス田 / DNA解析 / 自然トレーサー / 石油開発 / 微生物源追跡 / 油田 / 微生物解析 / マーカー
Outline of Research at the Start

微生物トレーサ法を地下の資源開発へ適用するための技術基盤を確立する。国内の油田を研究対象として,地下での微生物トレーサの挙動を,実証試験,微生物実験,流動シミュレーションにより包括的に明らかする。さらに,同技術の高度利用の概念実証を行う。

Outline of Final Research Achievements

Microbial DNA-based monitoring is a promising tool for environmental monitoring. To assess applicability of this tool on subsurface resource developments、 this study applied microbial DNA-based monitoring to an operating oilfield in Japan. Fluid samples were collected periodically from nine production wells and two injection wells in the Sarukawa oilfield (Alita prefecture) for three years. DNA was extracted from the samples、 and the microbial composition was analyzed. A comparative analysis of the microbial profiles suggested possible contribution of each reservoir formation to production、 reservoir compartmentation by faults、 well connectivity and water breakthrough、 demonstrating that microbial DNA-based monitoring can provide useful information for subsurface resource developments.

Academic Significance and Societal Importance of the Research Achievements

本研究は微生物トレーサ法の油田における世界初の長期実証研究であり、石油・天然ガスなど地下資源の開発における同手法の有用性が示された。地層水など地下から採取した試料に含まれる微生物叢を分析することで、地下の地層構造や流体の流動、微生物代謝など有用な情報が得られる事が示され、新規の地下環境モニタリング手法の技術基盤が確立された。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (8 results)

All 2023 Other

All Int'l Joint Research (4 results) Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (2 results) Remarks (1 results)

  • [Int'l Joint Research] スタンフォード大学(米国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] 中国科学院(中国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] スタンフォード大学(米国)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] 精華大学(中国)

    • Related Report
      2022 Research-status Report
  • [Journal Article] Long-Term Microbial DNA-Based Monitoring of the Mature Sarukawa Oil Field in Japan2023

    • Author(s)
      Kobayashi H.、Goto A.、Feng X.、Uruma K.、Momoi Y.、Watanabe S.、Sato K.、Zhang Y.、Horne R. N.、Shibuya T.、Okano Y.
    • Journal Title

      SPE Reservoir Evaluation & Engineering

      Volume: 26 Issue: 03 Pages: 1-10

    • DOI

      10.2118/214313-pa

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] DNAを用いた長期バイオモニタリング、申川油田の事例紹介2023

    • Author(s)
      後藤彩恵、漆間功真、桃井勇輝、渡辺駿一、渋谷岳史、岡野祥之、佐藤光三、小林肇
    • Organizer
      石油技術協会春季講演会
    • Related Report
      2023 Annual Research Report
  • [Presentation] DNAを用いた長期バイオモニタリング、申川油田の事例紹介2023

    • Author(s)
      後藤彩恵・漆間功真・桃井勇輝・渡辺駿一・渋谷岳史・岡野祥之・佐藤光三・小林肇
    • Organizer
      石油技術協会春季講演会
    • Related Report
      2022 Research-status Report
  • [Remarks] 小林研究室webページ

    • URL

      https://sites.google.com/site/hajimekobayashisys/home

    • Related Report
      2023 Annual Research Report 2022 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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