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Analysis of fatty acid-related signals contributing to the pathogenesis of bovine abomasum displacement and their application to early diagnostic markers

Research Project

Project/Area Number 21K05946
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

Tajima Tsuyoshi  日本獣医生命科学大学, 獣医学部, 講師 (60508878)

Co-Investigator(Kenkyū-buntansha) 三浦 亮太朗  日本獣医生命科学大学, 獣医学部, 准教授 (60782133)
金田 剛治  日本獣医生命科学大学, 獣医学部, 教授 (10350175)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords牛 / 第四胃変位 / n-3脂肪酸 / n-3/AA比 / TLR4シグナル / 血中脂肪酸 / エイコサペンタエン酸 / n-3/n-6脂肪酸比 / 中鎖脂肪酸 / ω-3脂肪酸 / 脂肪酸 / 代謝 / シグナル解析 / 診断マーカー
Outline of Research at the Start

分娩後の牛で好発する第四胃変位は発症後に乳量減少を伴うだけでなく死亡・廃用淘汰率も高いことから供用年数短縮の要因にもなっている。しかしこれまで変異した第四胃の組織で何がおこっているのかは不明であった。我々はこれまでに中鎖脂肪酸およびω-3脂肪酸が第四胃の収縮に影響を与える可能性を検討してきた。本課題では中鎖脂肪酸およびω-3脂肪酸の動態を分娩後継時的にモニタリングし、脂肪酸産生・代謝経路およびや脂肪酸/TLR4シグナルがどのように変化するかを理解することをめざす。また血中/第四胃組織中のアディポサイトカインにも着目し、関連するシグナルの増減が第四胃変位の早期診断マーカーとなり得るかも検討する。

Outline of Final Research Achievements

Blood samples were obtained from Holstein cows one week before calving and at 0-8 weeks after calving. The samples were analyzed to assess the blood fatty acid profile. A health check of the cows was also conducted to explore the relationship between abomasal displacement (DA) and ketosis, a known underlying pathological process of DA. The study investigated the correlation between DA and ketosis, which is considered the underlying factor in the development of DA.
The blood fatty acids level, including lauric acid, palmitic acid, and myristic acid, which act as endogenous toll-like receptor 4 (TLR4) activators, did not show significant differences between healthy cows and ketosis cows. However, blood levels of n-3 fatty acids (docosahexaenoic acid and docosapentaenoic acid) ratio vs arachidonic acid, which have a role in suppressing TLR4 signalling, decreased in ketosis cows within 1-2 weeks after calving.

Academic Significance and Societal Importance of the Research Achievements

第四胃平滑筋を用いたin vitro試験からTLR4シグナルの増強に関連する脂肪酸の分娩前後における血中動態について解析した。内因性TLR4リガンドとして働きうると推察されたラウリン酸、パルミチン酸、ミリスチン酸の血中濃度は健康牛・罹患牛ともに分娩前後で有意な変化はみられなかった。一方、n-3脂肪酸/アラキドン酸比が低下するとTLR4シグナル抑制機構の減弱に関与することから、DA発症に重要なTLRシグナル亢進の基盤として分娩直後にn-3脂肪酸が減少していることが寄与していると考えられた。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (6 results)

All 2023 2022

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 2 results) Presentation (3 results)

  • [Journal Article] Oral pharmacokinetics of sulfadiazine and sulfamonomethoxine in female Holstein milking cows2023

    • Author(s)
      TAJIMA Tsuyoshi、SAIGA Masumi、YAMAMOTO Haru、ELBADAWY Mohamed、ABUGOMAA Amira、MIURA Ryotaro、USUI Tatsuya、SASAKI Kazuaki、SHIMODA Minoru
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 85 Issue: 7 Pages: 715-720

    • DOI

      10.1292/jvms.23-0110

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prediction of the Spontaneous Estrus Expression Period Based on Large (?10 mm) Follicle Numbers in Lactating Holstein Dairy Cows2023

    • Author(s)
      Miura Ryotaro、Inoue Takuma、Kunugi Yuka、Yasukawa Miya、Koyama Saku、Sato Rena、Matsumura Tomochika、Tajima Tsuyoshi、Yoshimura Itaru、Ajito Tadaharu
    • Journal Title

      Veterinary Sciences

      Volume: 10 Issue: 3 Pages: 231-231

    • DOI

      10.3390/vetsci10030231

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of Prolonged Endometrial Inflammation Associated with the Periparturient Metabolic State in Dairy Cows2022

    • Author(s)
      Rena Sato, Saku Koyama, Miya Yasukawa, Takuma Inoue, Tomochika Matsumura, Asuka Kanazawa, Yukari Nozue, Yuriko Wada, Itaru Yoshimura, Yujiro Hagita, Hisashi Mizutani, Tsuyoshi Tajima, Tadaharu Ajito, Ryotaro Miura
    • Journal Title

      Animals

      Volume: 12 Issue: 23 Pages: 3401-3401

    • DOI

      10.3390/ani12233401

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Associations between postpartum uterine luminal fluid volume and long-term reproductive performance in lactating Holstein dairy cows.2023

    • Author(s)
      Ryotaro Miura, Yukari Nozue, Yujiro Hagita Tsuyoshi Tajima and Tadaharu Ajito
    • Organizer
      11th International Ruminant Reproduction Conference
    • Related Report
      2023 Annual Research Report
  • [Presentation] 黒毛和種リピートブリーダー牛における 繁殖開始時期の子宮内および糞便中の メタゲノム解析の特徴2023

    • Author(s)
      三浦亮太朗、萩田祐二朗、田島剛、味戸忠春、 濱野晴三、白砂孔明
    • Organizer
      日本畜産学会131回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 乳牛における子宮内膜の炎症収束時期と分娩前後の栄養代謝との関連2022

    • Author(s)
      三浦 亮太朗,佐藤 玲奈,小山 朔,安川 美弥,井上 拓馬,松村 智周,金澤 明日香, 野末 優花里,和田 有里子,吉村 格,萩田 祐二朗,水谷 尚,田島 剛,味戸 忠春
    • Organizer
      第115回 日本繁殖生物学会大会
    • Related Report
      2022 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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