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Molecular mechanism controlling polarity and morphogenesis of guard cells in plant

Research Project

Project/Area Number 21K06216
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionShimane University

Principal Investigator

Nakagawa Tsuyoshi  島根大学, 学術研究院農生命科学系, 教授 (30202211)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords気孔 / 孔辺細胞 / 孔辺母細胞 / 受容体型キナーゼ / 細胞極性 / シロイヌナズナ / ロイシンリッチリピート / 植物 / 遺伝子発現 / 突然変異体 / 形態構築
Outline of Research at the Start

細胞の分裂、伸長や変形など生物の形作りは細胞の極性に従って進行する。植物における細胞極性の重要性を示すユニークな例として気孔の形成が挙げられる。気孔を形成する2個の孔辺細胞は孔辺母細胞の対称な分裂によって生じ、その後それぞれの細胞が対称分裂面を挟んで背腹(外側-孔側)の極性を獲得して湾曲し、細胞の形態構築が進行する。本研究では我々が発見した孔辺細胞の背腹極性を制御する鍵因子シロイヌナズナMUSTACHES (MUS)受容体型キナーゼに着目した解析を進める。MUS受容体型キナーゼが働く信号伝達の仕組みをあきらかにし、植物細胞における極性形成の新たなメカニズムを提唱する。

Outline of Final Research Achievements

The MUS receptor-like kinase is expressed prior to division of guard mother cells and thought to function in establishing the polarity of guard cells. In this project, we explored the components of the MUS receptor-like kinase signaling pathway to understand the molecular mechanisms of signal transduction. Since it is known that leucine-rich repeat (LRR) receptor-like kinases function by forming complexes with other LRR receptor proteins, we conducted a semi-comprehensive expression analysis of Arabidopsis LRR receptor protein genes using promoter-reporter assay to identify genes expressed in the stomatal lineage. As a result, we discovered that the genes named AtExLRR1, AtExLRR3, and AtExLRR9 are expressed in the stomatal lineage.

Academic Significance and Societal Importance of the Research Achievements

地球環境にとって植物による光合成は重要な意味を持つ。光合成における植物内外のガス交換は気孔を通じて行われている。気孔形成、つまり葉の表面に孔をあけるためには、孔辺細胞が特殊な形となり、さらに2つの孔辺細胞が対称的に配置されなければならない。そのため孔辺細胞形態構築メカニズムの理解は植物発生分野における学術的意義に加え、地球環境にも深く関わる社会的意義を持つものである。本研究で着目しているMUS受容体型キナーゼ信号伝達系は、細胞板という植物に特有な分裂面形成を利用した巧妙な仕組みが使われている。生命活動の多様性の理解につながることからも社会的意義を持つものである。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (8 results)

All 2024 2023 2022 2021

All Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (4 results)

  • [Journal Article] Genetic and transcriptomic dissection of nitrate-independent function of Arabidopsis NRT1.1/NPF6.3/CHL1 under high ammonium condition2024

    • Author(s)
      Hachiya Takushi、Makita Nobue、Bach Lien、Gojon Alain、Nakagawa Tsuyoshi、Sakakibara Hitoshi
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: in press Issue: 5-6 Pages: 1-10

    • DOI

      10.1080/00380768.2024.2326086

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Expression analysis of genes encoding extracellular leucine-rich repeat proteins in <i>Arabidopsis thaliana</i>2023

    • Author(s)
      Dutta Amit Kumar、Sultana Mst Momtaz、Tanaka Ai、Suzuki Takamasa、Hachiya Takushi、Nakagawa Tsuyoshi
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 88 Issue: 2 Pages: 154-167

    • DOI

      10.1093/bbb/zbad171

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Targeted expression of bgl23-D, a dominant-negative allele of ATCSLD5, affects cytokinesis of guard mother cells and exine formation of pollen in Arabidopsis thaliana2023

    • Author(s)
      Hossain Md. Firose、Dutta Amit Kumar、Suzuki Takamasa、Higashiyama Tetsuya、Miyamoto Chiharu、Ishiguro Sumie、Maruta Takanori、Muto Yuki、Nishimura Kohji、Ishida Hideki、Aboulela Mostafa、Hachiya Takushi、Nakagawa Tsuyoshi
    • Journal Title

      Planta

      Volume: 257 Issue: 4 Pages: 64-82

    • DOI

      10.1007/s00425-023-04097-0

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Expression analysis of plant intracellular Ras-group related leucine-rich repeat proteins (PIRLs) in Arabidopsis thaliana2022

    • Author(s)
      Hossain Md. Firose、Sultana Mst Momtaz、Tanaka Ai、Dutta Amit Kumar、Hachiya Takushi、Nakagawa Tsuyoshi
    • Journal Title

      Biochemistry and Biophysics Reports

      Volume: 30 Pages: 101241-101241

    • DOI

      10.1016/j.bbrep.2022.101241

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Gatewayベクターにおけるネガティブコントロールコンストラクト作製システムの開発2024

    • Author(s)
      葛原大貴、門田宏太、蜂谷卓士、中川強
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 多様な遺伝子構築が可能なイネ形質転換システムの開発2024

    • Author(s)
      荒谷寧音、佐藤(志水)佐江、佐藤豊、蜂谷卓士、中川強
    • Organizer
      第65回日本植物生理学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Development of a vector system (Boost Gateway vector system) that facilitates the preparation of GAL4/UAS constructs and enhancement of expression with various promoter2022

    • Author(s)
      T. Nakagawa, M. Aboulela, Y. Yamada, S. Ishiguro, T. Hachiya, H. Tsukagoshi
    • Organizer
      第64回日本植物生理学会年会(仙台大会)
    • Related Report
      2022 Research-status Report
  • [Presentation] Expression analysis of Leucine-Rich Repeats Receptor-Like Kinases (LRR-RLKs) under different abiotic stresses in Arabidopsis thaliana2021

    • Author(s)
      Md. Firose Hossain, Takushi Hachiya, Tsuyoshi Nakagawa
    • Organizer
      第44回 日本分子生物学会年会
    • Related Report
      2021 Research-status Report

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Published: 2021-04-28   Modified: 2025-01-30  

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