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Molecular mechanism of translocation complex in P. falciparum gametocytes

Research Project

Project/Area Number 21K06994
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 49040:Parasitology-related
Research InstitutionNagasaki University

Principal Investigator

Miyazaki Shinya  長崎大学, 熱帯医学研究所, 助教 (40637435)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2022: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsマラリア / ガメトサイト / 骨髄間葉系幹細胞 / タンパク質輸送 / 熱帯熱マラリア原虫 / NanoLuc / トランスロコン / ノックダウン / マラリア原虫
Outline of Research at the Start

本研究では、ガメトサイト期トランスロコン複合体を構成する新規のタンパク質を同定し、それらがガメトサイト性分化に果たす役割を解明する。遺伝子改変の手法によりコンディショナルノックダウン原虫を作出し、ガメトサイト性分化に必須なトランスロコン構成分子を同定する。また、生化学の手法によりガメトサイト期トランスロコン複合体を単離精製し、その構成タンパク質を質量分析により網羅的に同定する。

Outline of Final Research Achievements

The aim of this study was to investigate the role of translocon-associated molecules in the gametocyte stage of Plasmodium falciparum. Through this study, we have gained knowledge on the genetic modification know-how using the gametocyte-producing strain NF54. Furthermore, new insights were obtained into the cytoadhesion of P. falciparum-infected erythrocytes to human bone marrow mesenchymal stem cells. We established an assay system to assess the cytoadhesion of P. falciparum-infected erythrocytes in the asexual blood stage to immortalised human bone marrow mesenchymal stem cells, and several candidate parasite adhesion ligand molecules were identified that could be involved in this phenomenon. This cytoadhesion phenomenon may be conserved not only in the asexual blood stage but also in the gametocyte stage. Future work will focus on the role of the identified molecules in the gametocyte stage.

Academic Significance and Societal Importance of the Research Achievements

本研究により、ガメトサイト期への性分化に深く関与する生物現象である骨髄間葉系幹細胞への接着現象を評価するための分子基盤が整った。骨髄細胞への接着にはトランスロコン複合体を介したタンパク質輸送が必須であると予想される。当初の計画とは異なるアプローチではあったが、目的のトランスロコン複合体が担う現象に注目し研究を進展させることができた。本研究は熱帯熱マラリア原虫のガメトサイト期がどのように分化を進めていくか、その分子基盤の一端を明らかにしガメトサイト生物学・抗マラリア薬開発における新たな知見を提供する。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (12 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (2 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Open Access: 4 results,  Peer Reviewed: 4 results) Presentation (3 results) (of which Invited: 1 results) Remarks (2 results)

  • [Int'l Joint Research] ライデン大学/TropIQ(オランダ)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] ソルボンヌ大学(フランス)

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Pivotal roles of Plasmodium falciparum lysophospholipid acyltransferase 1 in cell cycle progression and cytostome internalization2024

    • Author(s)
      Fukumoto Junpei、Yoshida Minako、Tokuoka Suzumi M.、Hayakawa Eri Saki H.、Miyazaki Shinya、Sakura Takaya、Inaoka Daniel Ken、Kita Kiyoshi、Usukura Jiro、Shindou Hideo、Tokumasu Fuyuki
    • Journal Title

      Biorexiv

      Volume: 2024.1.13 Pages: 59-59

    • DOI

      10.1101/2024.01.13.575495

    • Related Report
      2023 Annual Research Report
    • Open Access
  • [Journal Article] A versatile Plasmodium falciparum reporter line expressing NanoLuc enables highly sensitive multi-stage drug assays2023

    • Author(s)
      Miyazaki Yukiko、Vos Martijn W.、Geurten Fiona J. A.、Bigeard Pierre、Kroeze Hans、Yoshioka Shohei、Arisawa Mitsuhiro、Inaoka Daniel Ken、Soulard Valerie、Dechering Koen J.、Franke-Fayard Blandine、Miyazaki Shinya
    • Journal Title

      Communications Biology

      Volume: 6 Issue: 1 Pages: 713-713

    • DOI

      10.1038/s42003-023-05078-5

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plasmodium falciparum SURFIN4.1 forms an intermediate complex with PTEX components and Pf113 during export to the red blood cell2021

    • Author(s)
      Miyazaki Shinya、Chitama Ben-Yeddy Abel、Kagaya Wataru、Lucky Amuza Byaruhanga、Zhu Xiaotong、Yahata Kazuhide、Morita Masayuki、Takashima Eizo、Tsuboi Takafumi、Kaneko Osamu
    • Journal Title

      Parasitology International

      Volume: 83 Pages: 102358-102358

    • DOI

      10.1016/j.parint.2021.102358

    • NAID

      120007166820

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Screening of viral-vectored P. falciparum pre-erythrocytic candidate vaccine antigens using chimeric rodent parasites2021

    • Author(s)
      Kolli Surendra Kumar、Salman Ahmed M.、Ramesar Jai、Chevalley-Maurel Severine、Kroeze Hans、Geurten Fiona G. A.、Miyazaki Shinya、Mukhopadhyay Ekta、Marin-Mogollon Catherin、Franke-Fayard Blandine、Hill Adrian V. S.、Janse Chris J.
    • Journal Title

      PLOS ONE

      Volume: 16 Issue: 7 Pages: 0254498-0254498

    • DOI

      10.1371/journal.pone.0254498

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Creative interior design by Plasmodium falciparum: Lipid metabolism and the parasite's secret chamber2021

    • Author(s)
      Tokumasu Fuyuki、Hayakawa Eri H.、Fukumoto Junpei、Tokuoka Suzumi M.、Miyazaki Shinya
    • Journal Title

      Parasitology International

      Volume: 83 Pages: 102369-102369

    • DOI

      10.1016/j.parint.2021.102369

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 熱帯熱マラリア原虫感染赤血球の多様なヒト細胞への細胞接着を評価するためのプラットフォーム2024

    • Author(s)
      宮崎真也 , 田中萌, Ben-Yeddy Abel Chitama , 福本準平 , 吉田益奈子 , 徳舛冨由樹
    • Organizer
      第93回 日本寄生虫学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 創薬・ワクチン開発のための遺伝子組み換えマラリア原虫レポーターライン2022

    • Author(s)
      宮崎真也
    • Organizer
      第95回日本生化学会大会
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] A critical role of the apicoplast protein PALM for Plasmodium falciparum sporozoite development in the mosquito2021

    • Author(s)
      Shinya Miyazaki, Catherin Marin-Mogollon, Yukiko Miyazaki, Fiona J.A. Geurten, Jai Ramesar, Surendra Kumar Kolli, Severine Chevalley-Maurel, Chris J. Janse, Blandine Franke-Fayard
    • Organizer
      日本寄生虫学会総会
    • Related Report
      2021 Research-status Report
  • [Remarks] 長崎大が遺伝子改変熱帯熱マラリア原虫を創出 抗マラリア薬の開発へ進歩

    • URL

      https://news.mynavi.jp/techplus/article/20230724-2733899/

    • Related Report
      2023 Annual Research Report
  • [Remarks] マラリア創薬に向けた革新的ツールとなる遺伝子改変原虫を創出

    • URL

      https://www.nagasaki-u.ac.jp/ja/guidance/kouhou/press/file/2023/20230721-1-1.pdf

    • Related Report
      2023 Annual Research Report

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Published: 2021-04-28   Modified: 2025-01-30  

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