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Development of pulp regeneration for root canal treatment with dentin coating agent

Research Project

Project/Area Number 21K09968
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 57040:Regenerative dentistry and dental engineering-related
Research InstitutionNational Center for Geriatrics and Gerontology

Principal Investigator

IOHARA Koichiro  国立研究開発法人国立長寿医療研究センター, 研究所 ジェロサイエンス研究センター, 室長 (60435865)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2023: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2022: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords歯髄再生 / 象牙質処理 / 歯髄再生誘導象牙質コーティング方法 / 象牙質誘導 / 感染根管 / 象牙質コーティング
Outline of Research at the Start

感染根管治療薬剤による象牙質微小環境の変化を検討し、再生阻害因子を明らかにする。また、この象牙質微小環境を再生に適した状態に誘導する因子を明らかにし、歯髄再生誘導象牙質コーティング剤を開発する。最終的にイヌ感染根管治療歯髄再生モデルに応用し、有効性を検討する。

Outline of Final Research Achievements

The effects of chemicals on the dentin microenvironment in infected root canal therapy were clarified, and dentin coating materials suitable for pulp regeneration were developed. Toxicity tests showed that the calcium hydroxide and phenol formulations were toxic only on the surface, while the formalin formulation penetrated the entire dentin and was highly toxic. Cell attachment tests showed similar results. In the adipocyte induced pulp cell differentiation test, dentin EDTA-extracted protein was effective for inducing pulp regeneration. These results suggest that coatings with fibronectin, collagen, laminin, or EDTA-extracted dentin protein are highly recommended. However, in vivo studies did not show statistically significant results due to the effects of reinfection.

Academic Significance and Societal Importance of the Research Achievements

私たちはこれまで不可逆性歯髄炎に対する歯髄再生治療を開発し、臨床研究を行った。現在、この治療は特定再生医療等委員会の承認を得ることで開業医でも実施可能である。感染根管治療への適用を広げるために、根管の無菌化が必要で、水酸化カルシウム製剤を使用した治療では歯髄の再生が難しいことをあきらかにしていた。今回の結果より、水酸化カルシウム製剤とフェノール製剤は象牙質を一層削除することで歯髄再生が適した環境にできると考えられた。ホルマリン製剤は歯髄再生治療の際には使用しない方が良いと考えられた。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (14 results)

All 2024 2023 2022 2021

All Journal Article (5 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (9 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results)

  • [Journal Article] Periapical bacterial disinfection is critical for dental pulp regenerative cell therapy in apical periodontitis in dogs2024

    • Author(s)
      Iohara Koichiro、Tominaga Michiyo、Watanabe Hideto、Nakashima Misako
    • Journal Title

      Stem Cell Research & Therapy

      Volume: 15 Issue: 1

    • DOI

      10.1186/s13287-023-03628-6

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Periostin Is a Candidate Regulator of the Host Microenvironment in Regeneration of Pulp and Dentin Complex and Periodontal Ligament in Transplantation with Stem Cell-Conditioned Medium2024

    • Author(s)
      Shintarou Sakatoku, Yuki Hayashi, Taku Futenma, Yoshihiko Sugita, Ryo Ishizaka, Hiroyuki Nawa, Koichiro Iohara
    • Journal Title

      stem cells internationals

      Volume: e2024 Issue: 1 Pages: 7685280-7685280

    • DOI

      10.1155/2024/7685280

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Age Related Senescence, Apoptosis, and Inflammation Profiles in Periodontal Ligament Cells from Canine Teeth2022

    • Author(s)
      Zayed Mohammed、Iohara Koichiro
    • Journal Title

      Current Molecular Medicine

      Volume: 22 Issue: 8 Pages: 808-814

    • DOI

      10.2174/1566524022666220520124630

    • Related Report
      2022 Research-status Report
  • [Journal Article] Pulp Regenerative Cell Therapy for Mature Molars: A Report of 2 Cases2022

    • Author(s)
      Nakashima Misako、Fukuyama Fusanosuke、Iohara Koichiro
    • Journal Title

      Journal of Endodontics

      Volume: 48 Issue: 10 Pages: 1334-1340.e1

    • DOI

      10.1016/j.joen.2022.07.010

    • Related Report
      2022 Research-status Report
  • [Journal Article] Biological characteristics and pulp regeneration potential of stem cells from canine deciduous teeth compared with those of permanent teeth2022

    • Author(s)
      Ziauddin S. M.、Nakashima Misako、Watanabe Hideto、Tominaga Michiyo、Iohara Koichiro
    • Journal Title

      Stem Cell Research & Therapy

      Volume: 13 Issue: 1 Pages: 1-13

    • DOI

      10.1186/s13287-022-03124-3

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 根尖性歯周炎における歯髄再生治療を成功へ導く細菌検出法および移植時基準の検討2023

    • Author(s)
      庵原耕一郎,冨永三千代,中島美砂子
    • Organizer
      第159回日本歯科保存学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 歯髄幹細胞を用いた根管治療後の歯髄再生治療の実用化のための共同研究2023

    • Author(s)
      中島美砂子、庵原耕一郎
    • Organizer
      第22回日本再生医療学会総会 シンポジウム
    • Related Report
      2022 Research-status Report
    • Invited
  • [Presentation] 乳歯歯髄幹細胞を用いた同種移植による歯髄再生2023

    • Author(s)
      庵原耕一郎、中島美砂子、冨永三千代
    • Organizer
      第22回日本再生医療学会総会 ポスター発表
    • Related Report
      2022 Research-status Report
  • [Presentation] Periradicular disinfection is essential for pulp regeneration in the apical periodontitis.2022

    • Author(s)
      Iohara K, Ziauddin SM, Tominaga M, Nakashima M.
    • Organizer
      2022 IADR GENERAL SESSION and Exhibition Virtual Experience.
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Regenerative Potential of Stem Cells from Deciduous and Permanent Teeth.2022

    • Author(s)
      Ziauddin SM, Iohara K, Tominaga M, Nakashima M.
    • Organizer
      2022 IADR GENERAL SESSION and Exhibition Virtual Experience.
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 感染根管治療において除菌が困難な副根管へのプラス帯電性ナノバブルの効果 ―プラス帯電性ナノバブルの難治性感染根管治療への応用―2022

    • Author(s)
      庵原耕一郎、大平猛、冨永三千代、中島美砂子
    • Organizer
      日本マイクロ・ナノバブル学会 第10回学術総会
    • Related Report
      2022 Research-status Report
  • [Presentation] 「Recent Progress of Pulp Regenerative Therapy with Dental Pulp Stem Cells for the Application to the Periapical Disease in the Aged.」2021

    • Author(s)
      Koichiro Iohara
    • Organizer
      第69回国際歯科研究学会(JADR)日本部会総会・学術大会
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 「高齢者および根尖性歯周炎への歯髄幹細胞を用いた歯髄再生治療の最近の進歩 ―プラス帯電性ナノバブルの治療への応用―」2021

    • Author(s)
      庵原耕一郎、大平猛、Ziauddin SM、冨永三千代、中島美砂子
    • Organizer
      日本マイクロ・ナノバブル学会 第9回学会総会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] 「虫歯から歯を蘇らせる歯髄細胞Padによる新規象牙質再生」2021

    • Author(s)
      庵原 耕一郎
    • Organizer
      第21回日本再生医療学会総会 共催学術セミナー
    • Related Report
      2021 Research-status Report
    • Invited

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Published: 2021-04-28   Modified: 2025-01-30  

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