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Attempt of new electrical stimulation method for maintaining bone mass after sports injury and examination of its applicability

Research Project

Project/Area Number 21K11456
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 59020:Sports sciences-related
Research InstitutionToyo University

Principal Investigator

Ohsako Masafumi  東洋大学, 健康スポーツ科学部, 教授 (60152104)

Co-Investigator(Kenkyū-buntansha) 中井 真悟  常葉大学, 健康プロデュース学部, 講師 (10825540)
藤川 芳織  昭和大学, 歯学部, 助教 (60805943)
柴田 俊一  北海道医療大学, 歯学部, 客員教授 (80187400)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsラット / 後肢加重低減 / 骨量減少 / VP通電刺激 / 組織学的分析 / 免疫組織学的分析 / 形態計測 / 骨量維持効果 / ベクトルポテンシャル / 通電刺激 / 骨量維持 / 通電条件 / 加重低減 / 経皮通電 / 鍼通電 / VP通電 / 海綿骨骨密度 / 海綿骨骨梁幅 / ラット大腿骨 / 骨量 / 組織構造
Outline of Research at the Start

受傷したアスリートは競技への早期復帰を目指して、リハビリ初期からトレーニングに励むことが多い。しかし、運動が制限された状態でそれを進めなくはならなく、そこにトレーニングの限界があることから、運動せずに骨量維持を図れる可能性のある通電刺激法に期待が寄せられる。本研究では、最近開発されたベクトル・ポテンシャル装置を用いた通電刺激が、骨量や関節軟骨、オスグッド病が現れる脛骨粗面に及ぼす効果を検証する。

Outline of Final Research Achievements

In the first year, as a basic study, the effects of VP, acupuncture, and transcutaneous electrical stimulation on maintaining bone mass of the femur were compared and investigate using rats with their hindlimb suspended. As a result, bone mass was significantly reduced in the hindlimb suspended group, but bone mass was maintained in the electrical stimulation group, and the effect was particularly clear in the VP electrical stimulation group. In the second year, more effective electrical conditions for the VP electrical stimulation device were compared. A high frequency of intervention per week was effective, and effective results were obtained with intervention of 30 minutes or more per day. Higher voltage and frequency were also effective. In the last year, the possibility of VP electrical stimulation on tibial articular cartilage and knee joint capsule was examined, and it was found that VP electrical stimulation was effective in maintaining tissue structure in both tissues.

Academic Significance and Societal Importance of the Research Achievements

今日、高齢化に伴って骨粗鬆症、変形性膝関節症および関節拘縮の患者が増加の一途を辿っている。それらには薬物療法が広く行われているが、副作用があることも指摘されている。本研究は、動物実験によって、VP通電刺激が安全かつ有効な通電手段であることを証明し、それらの疾患の治療・予防に寄与し得る可能性を示唆した。また、この装置は周波数が高いほど装置の小型化が可能となるが、本研究では高い周波数領域でそれらの疾患の治療および予防効果が高いことが証明された。これらのことは社会実装を考える上でも本研究の成果が有効であることを意味する。これらの点に本研究の学術的および社会的意義があると思われる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (49 results)

All 2023 2022 2021

All Journal Article (13 results) (of which Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (31 results) (of which Int'l Joint Research: 8 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 3 results)

  • [Journal Article] Effects of Callus Formation during Bone Repair in Tibia Injured Rats on Hematopoietic Stem Cells Around the Callus2023

    • Author(s)
      Yashima Nao, Minamizono Wataru, Yang Chuwei, Ohsako Masafum
    • Journal Title

      Bull. Grad. School Toyo Univ,

      Volume: 59 Pages: 143-150

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Histological study in structure of cancellous bone at different site in growing rats2023

    • Author(s)
      Wataru, YASHIMA Nao, Xu Siqin, OHSAKO Masafumi
    • Journal Title

      Bull. Grad. School Toyo Univ

      Volume: 59 Pages: 185-196

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Eccentric contractions during downhill running induce Osgood-Schlatter disease in the tibial tuberosity in rats: a focus on histological structures2023

    • Author(s)
      Hirai Suito, Kaoru Fujikawa, Masafumi Ohsako
    • Journal Title

      Scientific Reports

      Volume: 13(1)

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Vector potential dual effect of promoting the proliferation of chondrocytes and inhibiting the calcification process in the articular cartilage2023

    • Author(s)
      Hirai Suito, Wataru Minamizono, Nao Yashima, Hiroya Matsunaga, Kaoru Fujikawa, Masafumi Ohsako
    • Journal Title

      Scientific Reports

      Volume: 13(1)

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ENPP1 downregulation and FGF23 upregulation in growth‐related calcification of the tibial tuberosity in rats2023

    • Author(s)
      Hirai Suito, Kaoru Fujikawa, Masafumi Ohsako
    • Journal Title

      J.Anatomy

      Volume: 224(2) Pages: 333-342

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 後肢不動化モデルラットの大腿骨骨折線に及ぼす鍼通電刺激効果の組織学的解析2023

    • Author(s)
      中井真悟、宇南山 伸、大迫正文
    • Journal Title

      全日本鍼灸学会雑誌

      Volume: 73(2) Pages: 121-130

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Study in effects of electrical stimulation of different intervention durations of intervention with electrical stimulation on structural changes of tibial articular cartilage induced by hind-limb suspension in rats2022

    • Author(s)
      ZENG, Xueqian, SUITOU, Hirai, MINAMIZONO, Wataru, YASHIMA,Nao, YANG,Chuwei, OHSAKO, Masafumi
    • Journal Title

      Bull. Grad. Sch. Toyo Univ.

      Volume: 57 Pages: 185-194

    • Related Report
      2022 Research-status Report
  • [Journal Article] Histological study on structural changes in articular capsule with growth in rats2022

    • Author(s)
      ANG,Chuwei,ZENG, Xueqian, SUITOU, Hirai, MINAMIZONO, Wataru, YASHIMA,Nao, OHSAKO, Masafumi
    • Journal Title

      Bull. Grad. Sch. Toyo Univ.

      Volume: 57 Pages: 221-246

    • Related Report
      2022 Research-status Report
  • [Journal Article] Effect of transcutaneous electrical stimulation of the different intervention frequency on bone structure of femur in hindlimb-suspended rats2021

    • Author(s)
      Minamizono,W.,Zeng, X.Q., Suitou,H., Yashima,N.,Young, C.W., Ohsako,M.
    • Journal Title

      Bull. Grad. Sch. Toyo Univ.

      Volume: 5656 Pages: 173-184

    • Related Report
      2021 Research-status Report
  • [Journal Article] Structural changes in attaching sites of tendon of femoral quadriceps muscle in growing rat2021

    • Author(s)
      Suitou H., Zeng X.Q., Minamizono W.,Ohsako M.
    • Journal Title

      Bull. Grad. Sch. Toyo Univ.

      Volume: 56 Pages: 185-194

    • Related Report
      2021 Research-status Report
  • [Journal Article] Effects of different forms of exercise on structures of tibial articular cartilage in rats2021

    • Author(s)
      Zeng X.Q.,Suitou H., Minamizono W.,Ohsako, M
    • Journal Title

      Bull. Grad. Sch. Toyo Univ.

      Volume: 5656 Pages: 195-207

    • Related Report
      2021 Research-status Report
  • [Journal Article] 加重低減に伴うラット大腿骨の構造変化に及ぼす異なる周波数の通電刺激の影響2021

    • Author(s)
      小林宗弘、望月将希、大迫正文
    • Journal Title

      ライフデザイン学研究

      Volume: 1616 Pages: 131-140

    • NAID

      120007035447

    • Related Report
      2021 Research-status Report
  • [Journal Article] 後肢懸垂ラットの異なる部位の骨構造に及ぼす 経皮通電刺激の影響2021

    • Author(s)
      望月将希、小林宗弘、大迫正文
    • Journal Title

      ライフデザイン学研究

      Volume: 1616 Pages: 195-203

    • NAID

      120007035438

    • Related Report
      2021 Research-status Report
  • [Presentation] Effect of VP electrcal stimulation on healing of bone injury in rats' tibias2023

    • Author(s)
      Nao Yashima, Hirai Suito, Wataru Minamizono, Masafumi Ohsako
    • Organizer
      28th Annual Congress of the European College of Sport Science
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of noncontact electrical stimulation under different conditions on bone structures of mechanical unloading rats2023

    • Author(s)
      Wataru Minamizono, Nao Yashima, Masafumi Osako
    • Organizer
      28th Annual Congress of the European College of Sport Science
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 短期間の通電刺激がラット関節軟骨の石灰化に及ぼす効果2023

    • Author(s)
      門間天空, 水藤飛来, 南園 航, 八嶋奈央, 大迫正文
    • Organizer
      第128回日本解剖学会学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 異なる時間のベクトルポテンシャル刺激が荷重低減に伴う骨量減少に及ぼす効果2023

    • Author(s)
      南園 航、八嶋奈央、徐 思琴、大迫正文
    • Organizer
      第128回日本解剖学会学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 骨折を想定した骨損傷モデルラットにおける腰椎体皮質骨の組織学的特徴2023

    • Author(s)
      八嶋奈央, 南園航、徐思琴、大迫正文
    • Organizer
      第128回日本解剖学会学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 発育期ラットの骨端板の軟骨細胞における雌雄差に関する研究2023

    • Author(s)
      徐 思琴、八嶋奈央、南園航、大迫正文
    • Organizer
      第128回日本解剖学会学術大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Effects of Noncontact Current Stimulation on Bone Healing in a Rat Leg Fracture Model2023

    • Author(s)
      Nao Yashima, Wataru Minamizono, Siqin Xu, Hirai Suito, Masafumi Ohsako
    • Organizer
      日本骨代謝学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 後肢加重低減ラットにおける大腿骨の構造に及ぼす高周波数の通電刺激の効果2023

    • Author(s)
      南園航, 水藤飛来, 八嶋奈央, 松永拓也, 中井真悟, 大迫正文
    • Organizer
      第32回日本柔道整復接骨医学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ラット脛骨骨端部における骨損傷が遠隔に位置する腰椎椎体の皮質骨に及ぼす影響2023

    • Author(s)
      八嶋奈央, 水藤飛来, 南園航, 松永拓也, 中井真悟, 大迫正文
    • Organizer
      第32回日本柔道整復接骨医学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 発育に伴うラット関節軟骨の組織学的構造変化ー石灰化層の変化を中心に2023

    • Author(s)
      松永拓也, 水藤飛来, 南園航, 八嶋奈央, 中井真悟, 大迫正文
    • Organizer
      第32回日本柔道整復接骨医学会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 異なる電流による通電刺激が後肢荷重低減下のラット大腿骨の構造に及ぼす影響2022

    • Author(s)
      南園航、八嶋奈央、徐思琴、大迫正文
    • Organizer
      日本体力医学会第77回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 後肢荷重低減が幼若ラットの大腿骨骨頭の構造に及ぼす影響2022

    • Author(s)
      八嶋奈央、南園航、徐思琴、大迫正文
    • Organizer
      日本体力医学会第77回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Effects of noncontact electrical stimulation under different electrical conditions on bone structure of mechanical unloading rats2022

    • Author(s)
      Zeng,X. Q. , H. Suito, , W. Minamizono, N. Yashima, C. Yang, K. Fujikawa, M. Ohsako
    • Organizer
      European College Sport Science
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effect of electrical stimulation at different frequencies on the structure of tibial articular cartilage in hindlimb-suspended rats2022

    • Author(s)
      Zeng,X. Q. , H. Suito, , W. Minamizono, N. Yashima, C. Yang, K. Fujikawa, M. Ohsako
    • Organizer
      European College Sport Science
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 後肢懸垂ラットによる異なる介入頻度の経皮通電刺激がラット大腿骨の骨構造に及ぼす影響に関する研究2021

    • Author(s)
      南園 航、曾 雪倩、水藤飛来、岩本紗由美、大迫正文
    • Organizer
      第126回日本解剖学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 下りトレッドミル走運動によるラット脛骨粗面の構造変化に物理刺激が与える影響2021

    • Author(s)
      水藤飛来、南園 航、中井真悟、大迫正文
    • Organizer
      第29回柔道整復接骨医学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 加重低減に伴う骨構造の変化に及ぼす経皮通電刺激の効果2021

    • Author(s)
      南園 航、水藤飛来、中井真悟、大迫正文
    • Organizer
      第29回柔道整復接骨医学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 下りトレッドミル走による発育期ラット脛骨粗面の構造変化に対する経皮通電刺激の影響2021

    • Author(s)
      水藤飛来、曾 雪倩、南園 航、 鈴木哲郎 、岩本紗由美 、大迫正文
    • Organizer
      第126回日本解剖学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 後肢懸垂に伴うラット脛骨関節軟骨の構造変化に及ぼす通電刺激の影響2021

    • Author(s)
      曾 雪倩、水藤飛来、南園 航、 鈴木哲郎、大迫正文
    • Organizer
      第126回日本解剖学会
    • Related Report
      2021 Research-status Report
  • [Presentation] Effects of nonattached electrical stimulation on structures of lumbar vertebrate in hindlimb -suspended rats2021

    • Author(s)
      Ohsako,M., Zeng,X.-Q., Suito,H., Minamizono,W., Yashima,N., Yang.C.
    • Organizer
      26th European College of Sport Medicine
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Study in effects of different periods of interventions with electrical stimulation on structural changes of tibial articular cartilage induced by hind-limb suspension in rats2021

    • Author(s)
      Zeng, X.-Q., Suito,H., Minamizono,W., Yashima,N., Yang.C. Ohsako,M.
    • Organizer
      26th European College of Sport Medicine
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Effect of transcutaneous electrical stimulations to different sites after down-hill treadmill running on the tissue structure of tibial tuberosity in rats2021

    • Author(s)
      Suito,H., Zeng, X.-Q., Minamizono,W., Yashima,N., Yang.C. Nakai,S., Fujikawa,K. ., Iwamoto,S., Ohsako,M.
    • Organizer
      26th European College of Sport Medicine
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Study in effects of contact and no-contact electrical stimulations on structural changes of femur caused by mechanical unloading in rats2021

    • Author(s)
      Minamizono,W.,Zeng, X.-Q., Suito,H., Yashima,N., Yang.C. Ohsako,M.
    • Organizer
      26th European College of Sport Medicine
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 加重低減によって引き起こされるラット脛骨関節軟骨の構造変化に対する異なる介入時間の通電刺激の影2021

    • Author(s)
      曾 雪倩、水藤飛来、南園 航、楊 楚薇、八島奈央、大迫正文
    • Organizer
      第78回日本体力医学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 上りおよび下りトレッドミル走がラット脛骨粗面の石灰化過程に及ぼす影響2021

    • Author(s)
      水藤飛来、曾 雪倩、南園 航、楊 楚薇、八島奈央、岩本 紗由美、大迫正文
    • Organizer
      第78回日本体力医学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 後肢加重低減に伴うラット大腿骨の初期の構造変化に対する非接触性通電刺激の影響2021

    • Author(s)
      南園 航、水藤飛来、曾 雪倩、楊 楚薇、八島奈央、大迫正文
    • Organizer
      第78回日本体力医学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 加重低減に伴うラット脛骨関節軟骨の構造変化に及ぼす異なる介入時間の通電刺激の影響2021

    • Author(s)
      曾 雪倩、水藤飛来、南園 航、楊 楚薇、八島奈央、大迫正文
    • Organizer
      第39回日本骨代謝学会
    • Related Report
      2021 Research-status Report
  • [Presentation] ラット膝蓋靭帯埋入部の発育に伴う構造変化2021

    • Author(s)
      水藤飛来、曾 雪倩、南園 航、楊 楚薇、八島奈央、大迫正文
    • Organizer
      第39回日本骨代謝学会
    • Related Report
      2021 Research-status Report
  • [Presentation] ベクトルポテンシャル発生装置を用いた通電刺激によるラットの骨量維持の試み2021

    • Author(s)
      南園 航、水藤飛来、曾 雪倩、陽 楚薔、八島奈央、大迫正文
    • Organizer
      第23日本骨粗鬆症学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 発育に伴うラット膝側副靭帯の構造変化に関する研究2021

    • Author(s)
      八島奈央、曽 雪倩、水藤飛来、南園 航、楊 楚薇、大迫正文
    • Organizer
      第78回日本体力医学会
    • Related Report
      2021 Research-status Report
  • [Presentation] 発育に伴うラット膝関節包の構造変化に関する研究2021

    • Author(s)
      楊 楚薇、曽 雪倩、水藤飛来、南園 航、八島奈央、大迫正文
    • Organizer
      第78回日本体力医学会
    • Related Report
      2021 Research-status Report
  • [Patent(Industrial Property Rights)] U.S. Patent Application2023

    • Inventor(s)
      Masafumi OHSAKO,
    • Industrial Property Rights Holder
      Masafumi OHSAKO,
    • Industrial Property Rights Type
      特許
    • Filing Date
      2023
    • Related Report
      2023 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 特許願2023

    • Inventor(s)
      大迫正文
    • Industrial Property Rights Holder
      大迫正文
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2023-066163
    • Filing Date
      2023
    • Related Report
      2023 Annual Research Report
  • [Patent(Industrial Property Rights)] 脂肪細胞の数を制御する方法2023

    • Inventor(s)
      斎藤正樹、大迫正文
    • Industrial Property Rights Holder
      斎藤正樹、大迫正文
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2023-066163
    • Filing Date
      2023
    • Related Report
      2022 Research-status Report
  • [Patent(Industrial Property Rights)] METHOD FOR TREATING LIVING BODY USING ELECTRICAL STIMULATOR2022

    • Inventor(s)
      K.TERAO, M.OHSAKO, X.Q.ZENG
    • Industrial Property Rights Holder
      K.TERAO, M.OHSAKO, X.Q.ZENG
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2022-027532
    • Filing Date
      2022
    • Related Report
      2022 Research-status Report
    • Overseas
  • [Patent(Industrial Property Rights)] 電気刺激装置の作動方法及び電気刺激装置を用いる生体治療方法2022

    • Inventor(s)
      大迫正文、曽 雪倩、金子卓樹、斎藤正、デトモト、寺尾健治
    • Industrial Property Rights Holder
      大迫正文、曽 雪倩、金子卓樹、斎藤正、デトモト、寺尾健治
    • Industrial Property Rights Type
      特許
    • Filing Date
      2022
    • Related Report
      2021 Research-status Report
    • Overseas

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Published: 2021-04-28   Modified: 2025-01-30  

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