• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Cell Imaging Technique by Electrical Impedance Electro-Rotation Tomography

Research Project

Project/Area Number 21K14072
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 19010:Fluid engineering-related
Research InstitutionChiba University

Principal Investigator

Kawashima Daisuke  千葉大学, 大学院工学研究院, 特任助教 (10813785)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2021: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords電気トモグラフィ / 誘電回転 / 細胞イメージング / 逆問題解析 / 電気インピーダンストモグラフィ / 細胞 / イメージング
Outline of Research at the Start

本研究は、細胞の内部構造や細胞を構成する細胞膜・小器官をイメージングするために、細胞の誘電回転制御(ERC)と電気インピーダンス・トモグラフィ(EIT)を統合した電気インピーダンス・誘電回転トモグラフィ(EIeRT)の開発を目的とする。本提案のEIeRTは、細胞の回転角の高精度な制御を実現することで、細胞と電極の相対位置を精密に変化させ、これにより、インピーダンス計測の電極ペア数を増加させ、EITによる画像再構成技術と組み合わせることで高解像度な細胞イメージングを実現する。

Outline of Final Research Achievements

This study has built an electrical impedance electrorotation tomography (EIeRT) system. A numerical analysis model for controlling cell rotation has been constructed by coupling the motion equations of cell rotation and fluid dynamics. Also, a microelectrode array sensor (EIeRT sensor) capable of controlling cell rotation and measuring impedance has been designed and developed. By utilizing this system, we have successfully achieved high-resolution imaging of single cells, capturing details of the cytoplasm and cell nucleus. This accomplishment represents a significant milestone in establishing the foundation for non-invasive real-time cell monitoring.

Academic Significance and Societal Importance of the Research Achievements

本研究では、誘電回転を利用した細胞回転制御に対する数値解析モデルを構築し、新たな細胞制御技術に関する物理的な知見を提供した。また、本イメージング手法の実現により、非侵襲リアルタイム・細胞モニタリングへの応用が期待され、細胞の内部構造や細胞を構成する細胞膜・小器官の性状を可視化する細胞イメージングにより、疾患や薬物の細胞動態を解析技術として、医療・創薬分野における革新的な技術としての基盤を築いた。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (12 results)

All 2022 2021 Other

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] Detection of Heterogeneous Cells in Cell Spheroids by Applying High-Frequency Second-Order Sensitivity Matrix Electrical Impedance Tomography (HSSM-EIT)2022

    • Author(s)
      Li Songshi、Kawashima Daisuke、Gao Zengfeng、Takei Masahiro
    • Journal Title

      IEEE Open Journal of Instrumentation and Measurement

      Volume: 1 Pages: 1-9

    • DOI

      10.1109/ojim.2022.3212753

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multiple Regression Fitting Electrical Impedance Spectro-Tomography for Quantitative Image Reconstruction of Dead Cell Fraction and Cell Concentration2022

    • Author(s)
      Kawashima Daisuke、Obara Hiromichi、Takei Masahiro
    • Journal Title

      IEEE Open Journal of Instrumentation and Measurement

      Volume: 1 Pages: 1-8

    • DOI

      10.1109/ojim.2022.3198476

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Non-invasive imaging of ion concentration distribution around cell spheroids by electrical impedance tomographic sensor printed on circuit board under temporal compensation by ion transport impedance model2022

    • Author(s)
      Kawashima Daisuke、Yuki Tsubasa、Li Songshi、Takei Masahiro
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 212 Pages: 114432-114432

    • DOI

      10.1016/j.bios.2022.114432

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Study of transmembrane ion transport under tonicity imbalance using a combination of low frequency-electrical impedance spectroscopy (LF-EIS) and improved ion transport model2022

    • Author(s)
      Li Songshi、Kawashima Daisuke、Sugawara Michiko、Obara Hiromichi、Okeyo Kennedy Omondi、Takei Masahiro
    • Journal Title

      Biomedical Physics & Engineering Express

      Volume: - Issue: 3 Pages: 035024-035024

    • DOI

      10.1088/2057-1976/ac5fc5

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Evaluation of transmembrane ion transport via ion channel by low-frequency electrical impedance tomography2022

    • Author(s)
      Daisuke Kawashima, Songshi Li, Masahiro Takei
    • Organizer
      9th World Congress of Biomechanics 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] マイクロ電気インピーダンストモグラフィーを利用した単一細胞の可視化2022

    • Author(s)
      李 淞什, 川嶋 大介, 青木 伸之, 武居 昌宏
    • Organizer
      日本機械学会 第33回バイオフロンティア講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 二次感度行列を利用した電気インピーダンストモグラフィーによる異種細胞混合スフェロイドの検出2022

    • Author(s)
      李 淞什, 川嶋 大介, 武居 昌宏
    • Organizer
      日本機械学会第34回バイオエンジニアリング講演会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Imaging of ion concentration around cell spheroid by electrical impedance tomography2021

    • Author(s)
      Daisuke Kawashima, Tsubasa Yuki, Songshi Li, hiromichi Obara, Masahiro Takei
    • Organizer
      The 11th Asian-Pacific Conference on Biomechanics
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Cell identification based on ion transmembrane transport under tonicity imbalance by low frequency-electrical impedance2021

    • Author(s)
      Songshi Li, Daisuke Kawashima, Michiko Sugawara, Hiromichi Obara, Masahiro Takei
    • Organizer
      The 11th Asian-Pacific Conference on Biomechanics
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 電気インピーダンストモグラフィを利用した細胞スフェロイド周囲のイオン拡散の定量分析2021

    • Author(s)
      川嶋大介, 結城翼, 李淞什, 小原弘道, 武居昌宏
    • Organizer
      可視化シンポジウム2021
    • Related Report
      2021 Research-status Report
  • [Presentation] Application of Electrical Impedance Spectroscopy and Tomography to Cell Measurement and Evaluation2021

    • Author(s)
      Daisuke Kawashima
    • Organizer
      The 5th Africa International Biotechnology and Biomedical Conference (AIBBC 2021)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Remarks] 千葉大学武居研究室

    • URL

      https://tomocloud.xsrv.jp/takei-lab/

    • Related Report
      2022 Annual Research Report

URL: 

Published: 2021-04-28   Modified: 2024-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi