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Below-ground biodiversity assessment using soil nematodes for conversion of cedar plantations into broad-leaved forests

Research Project

Project/Area Number 21K14876
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 40010:Forest science-related
Research InstitutionMie University

Principal Investigator

Kitagami Yudai  三重大学, 生物資源学研究科, 助教 (40882684)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords生物指標 / スギ人工林 / 広葉樹林化 / 土壌線虫 / ポット実験 / ネットワーク解析 / 土壌微生物 / メタゲノム解析 / 土壌化学性 / 広葉樹林
Outline of Research at the Start

本研究はスギ人工林から広葉樹林へ転換後の地下部多様性の変化を示すため,線虫を用いた生物指標の確立を目指す.はじめに,スギ人工林と広葉樹林の線虫群集の特徴を把握する.次に,室内ポット実験で,スギ林土壌に広葉樹を植栽し,植栽後の線虫群集やその餌資源である微生物の動態を解明する.最後に,スギ皆伐地に広葉樹を植栽し,線虫群集指数を用いて地下部多様性をモニタリングすることで,広葉樹林化後の土壌の生態系機能の評価を試みる.

Outline of Final Research Achievements

The aim of this study was to establish nematodes as bio-indicator for evaluating changes in below-ground biodiversity by conversion of cedar plantations to broad-leaved forests. Soil nematode communities were different between cedar plantations and broad-leaved forests. Nematodes that feed on plant roots were dominant in the cedar plantations, and ones that eat fungal-mycelium were dominant in the broad-leaved forests. Moreover, nematode communities were affected by soil pH, carbon and nitrogen content. These results suggested that conversion of tree species alters the soil environment and affects below-ground nematode communities, and that nematodes respond sensitively to changes in the above-ground environment.

Academic Significance and Societal Importance of the Research Achievements

森林の多面的機能を発揮するために,放棄されたスギ人工林を豊かな広葉樹林に誘導する試みがある.広葉樹林の多面的機能として生物多様性の保全が期待されるが,そのためには物質循環に寄与する地下部の生物群集をモニタリングする適切な生物指標が求められる.森林土壌において線虫は環境変化に鋭敏に応答し様々な生態系における生物指標になることから,線虫は広葉樹林化後の地下部多様性の変化を適切にモニタリングできる生物指標になると考えた.本研究において,土壌線虫が森林タイプの違いに応答したことから,地上部環境の改変に対して地下部多様性の変化を評価する生物指標としての利用可能性を示した.

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (24 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results) Presentation (13 results) (of which Int'l Joint Research: 1 results) Remarks (3 results)

  • [Int'l Joint Research] Division of Botanical Garden/Taiwan Forestry Research Institute(その他の国・地域)

    • Related Report
      2022 Research-status Report
  • [Journal Article] Effects of tree species identity and soil origin on soil nematode communities and trophic composition in coniferous and broad-leaved forests.2024

    • Author(s)
      Yudai KItagami, Kohei Suzuki, Yosuke Matsuda
    • Journal Title

      Plant and Soil

      Volume: - Issue: 1-2 Pages: 489-502

    • DOI

      10.1007/s11104-024-06599-6

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution and characterization of nematodes in above-ground microhabitats in a natural pristine cedar forest in Yakushima Island, Japan2024

    • Author(s)
      Kitagami Yudai、Matsuda Yosuke
    • Journal Title

      Canadian Journal of Zoology

      Volume: 102 Issue: 3 Pages: 264-271

    • DOI

      10.1139/cjz-2023-0107

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Review of the Current Status and Perspectives of Nematode Communities in Forest Ecosystems2023

    • Author(s)
      Kitagami Yudai
    • Journal Title

      Journal of the Japanese Forest Society

      Volume: 105 Issue: 4 Pages: 136-146

    • DOI

      10.4005/jjfs.105.136

    • ISSN
      1349-8509, 1882-398X
    • Year and Date
      2023-04-30
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of climatic and edaphic conditions on structuring patterns of soil nematode communities in Japanese cedar (Cryptomeria japonica) plantations2022

    • Author(s)
      Kitagami Yudai、Obase Keisuke、Chen Chien-Fan、Matsuda Yosuke
    • Journal Title

      Forest Ecology and Management

      Volume: 524 Pages: 120518-120518

    • DOI

      10.1016/j.foreco.2022.120518

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] High-throughput sequencing covers greater nematode diversity than conventional morphotyping on natural cedar forests in Yakushima Island, Japan2022

    • Author(s)
      Kitagami Yudai、Matsuda Yosuke
    • Journal Title

      European Journal of Soil Biology

      Volume: 112 Pages: 103432-103432

    • DOI

      10.1016/j.ejsobi.2022.103432

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] High-throughput sequencing and conventional morphotyping show different soil nematode assemblages but similar community responses to altitudinal gradients on Mt. Ibuki, Japan2022

    • Author(s)
      Kitagami Yudai、Obase Keisuke、Matsuda Yosuke
    • Journal Title

      Pedobiologia

      Volume: 90 Pages: 150788-150788

    • DOI

      10.1016/j.pedobi.2021.150788

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effect of ectomycorrhizal fungal species on population growth and food preference of a fungivorous nematode2022

    • Author(s)
      Kitagami Yudai、Matsuda Yosuke
    • Journal Title

      Mycorrhiza

      Volume: 32 Issue: 1 Pages: 95-104

    • DOI

      10.1007/s00572-021-01063-0

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] 針葉樹林と広葉樹林の土壌細菌・真菌・線虫群集構造と共起ネットワーク構造2024

    • Author(s)
      北上雄大・松田陽介
    • Organizer
      第135回日本森林学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 針葉樹人工林と広葉樹二次林の土壌と樹種の入れ替えが土壌線虫群集に与える影響2023

    • Author(s)
      北上雄大・松田陽介
    • Organizer
      第45回日本土壌動物学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 異なる高さのスギ樹皮に生息する線虫の分子系統学的位置と群集構造2023

    • Author(s)
      松村龍一・北上雄大・山中豪・松田陽介
    • Organizer
      第30回日本線虫学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] スギ人工林と広葉樹二次林の境界部の土壌線虫群集構造2023

    • Author(s)
      今尾陽太・北上雄大・山中豪・松田陽介
    • Organizer
      第30回日本線虫学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] ササクレヒトヨタケの線虫捕捉器官の形態学的特徴2023

    • Author(s)
      竹田奏斗・北上雄大・井上伸・松田陽介
    • Organizer
      第30回日本線虫学会大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Effects of climatic and edaphic conditions on structuring patterns of soil nematode communities in Japanese cedar (Cryptomeria japonica) plantations2023

    • Author(s)
      Kitagami Y・Obase K・Chen CF・Matsuda Y
    • Organizer
      The 10th East Asian Federation of Ecological Societies International Congress
    • Related Report
      2023 Annual Research Report
  • [Presentation] スギ林とコナラ林の土壌と樹種の入れ替えが 土壌生物群集に与える影響2023

    • Author(s)
      北上雄大・鈴木康平・松田陽介
    • Organizer
      第134回日本森林学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] DNAメタバーコーディング法と形態観察法による屋久島スギ天然林の土壌線虫群集の比較2022

    • Author(s)
      北上雄大・松田陽介
    • Organizer
      第44回日本土壌動物学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 真菌食性線虫の増殖に関わるスギ細根由来の内生菌の餌資源特性2022

    • Author(s)
      河村実紀・北上雄大・松田陽介
    • Organizer
      第12回中部森林学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 異なる高さのスギ樹皮に生息する線虫の群集構造2022

    • Author(s)
      松村龍一・北上雄大・松田陽介
    • Organizer
      第12回中部森林学会大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Effect of ectomycorrhizal fungal species on population growth and food preference of a fungivorous nematode2022

    • Author(s)
      Kitagami Y・Matsuda Y
    • Organizer
      The 11th International Conference on Mycorrhiza
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Diversity and assemblage patterns of soil nematodes in Cryptomeria japonica plantations2022

    • Author(s)
      Kitagami Y・Obase K・Tanikawa T・Chen CF・Matsuda Y
    • Organizer
      第133回日本森林学会大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 外生菌根菌4種が真菌食性線虫Aphelenchoides sp.の個体数と餌選択性に及ぼす影響2021

    • Author(s)
      北上雄大・松田陽介
    • Organizer
      第28回日本線虫学会大会
    • Related Report
      2021 Research-status Report
  • [Remarks] researchmap

    • URL

      https://researchmap.jp/ykitagami

    • Related Report
      2023 Annual Research Report 2022 Research-status Report
  • [Remarks] 北上雄大(Yudai Kitagami) ホームページ

    • URL

      https://y-kitagami-nema.jimdofree.com

    • Related Report
      2021 Research-status Report
  • [Remarks] 森林微生物学 - 三重大学生物資源学部

    • URL

      https://www.bio.mie-u.ac.jp/junkan/forest/mycology2/

    • Related Report
      2021 Research-status Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

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