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Regulation of light-harvesting systems in photosystem II supercomplexes

Research Project

Project/Area Number 21K15129
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Kim Eunchul  基礎生物学研究所, 環境光生物学研究部門, 助教 (30836359)

Project Period (FY) 2021-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords光合成 / 集光 / 光防御 / 光化学系II / 光化学系I / 光化学系メガ複合体 / メガ複合体 / 配列形態 / 光化学系 / 光化学系配列形態
Outline of Research at the Start

植物は変動する光条件に対して、効率的な光合成を維持するため光合成機構を調節する多様なメカニズムを持っている。その中、チラコイド膜中の光化学系II-集光装置超複合体(PSII超複合体)が配列形態を変容させることにより、光合成のための集光機能を調節していることが最近明らかになった。しかし、どのような調節因子がこの配列形態を変容させるかなどのメカニズムはまだ明らかにされていない。本研究では、PSII超複合体の配列形態を変容させる因子を明らかにし、そのメカニズムを解明することを目的とする。本研究成果により、PSII超複合体の集光性質を調節する配列形態の変容メカニズムが明らかになると期待される。

Outline of Final Research Achievements

Photosystem I (PSI) and Photosystem II (PSII) in green plants form light-harvesting complexes and megacomplexes (PSI-PSII and PSII-PSII) to regulate light-harvesting properties. This study revealed that factors such as pH, PsbS, temperature, and light influence the arrangement of the photosynthetic system and the formation of megacomplexes. Furthermore, it was found that different plant species exhibit variations. A. thaliana forms both megacomplexes, while Spinach prefers the formation of PSII-PSII megacomplexes, and C. reinhardtii does not form stable megacomplexes. Additionally, it was evident that the PSI-PSII megacomplex derived from O. sativa exhibits stable and slower spillover compared to A. thaliana. This research elucidates the regulation factors and diversity of green plant photosynthetic megacomplexes.

Academic Significance and Societal Importance of the Research Achievements

光合成は地球上の生態系の保全に重要な役割を果たし、再生可能エネルギーの源としても注目されている。この研究により、光化学系における配列形態やメガ複合体の形成に影響を与える因子が明らかになり、緑色植物の光合成制御メカニズムを理解する上で重要である。さらに、異なる植物種が独自の光捕集機構を持っていることが示唆され、植物の進化と適応に関する理解を深めることができる。また、この研究の成果は光合成メカニズムの理解を深め、より効率的な再生可能エネルギーの開発に貢献することが期待される。このように、本研究の成果は基礎科学の進歩だけでなく、環境保全やエネルギー技術の発展にも重要な貢献をする研究成果である。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (14 results)

All 2023 2022 2021

All Journal Article (8 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 7 results,  Open Access: 4 results) Presentation (6 results) (of which Int'l Joint Research: 4 results)

  • [Journal Article] Photosynthetic Fluorescence from Earthlike Planets around Sunlike and Cool Stars2023

    • Author(s)
      Komatsu Yu、Hori Yasunori、Kuzuhara Masayuki、Kosugi Makiko、Takizawa Kenji、Narita Norio、Omiya Masashi、Kim Eunchul、Kusakabe Nobuhiko、Meadows Victoria、Tamura Motohide
    • Journal Title

      The Astrophysical Journal

      Volume: 942 Issue: 2 Pages: 57-57

    • DOI

      10.3847/1538-4357/aca3a5

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Regulation of light-harvesting systems in plants: Flexible conformation of light-harvesting complex II trimer depending on its binding site2023

    • Author(s)
      Kim Eunchul、Kubota-Kawai Hisako、Kawai Fumihiro、Yokono Makio、Minagawa Jun
    • Journal Title

      Biophysical Journal

      Volume: 122 Issue: 3 Pages: 242a-242a

    • DOI

      10.1016/j.bpj.2022.11.1411

    • Related Report
      2022 Annual Research Report
  • [Journal Article] The photosystem I supercomplex from a primordial green alga Ostreococcus tauri harbors three light-harvesting complex trimers2023

    • Author(s)
      Ishii Asako、Shan Jianyu、Sheng Xin、Kim Eunchul、Watanabe Akimasa、Yokono Makio、Noda Chiyo、Song Chihong、Murata Kazuyoshi、Liu Zhenfeng、Minagawa Jun
    • Journal Title

      eLife

      Volume: 12

    • DOI

      10.7554/elife.84488

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Overexpression of LHCSR and PsbS enhance light tolerance in Chlamydomonas reinhardtii2023

    • Author(s)
      Wilson Sam、Kim Eunchul、Ishii Asako、Ruban Alexander V.、Minagawa Jun
    • Journal Title

      Journal of Photochemistry and Photobiology B: Biology

      Volume: - Pages: 112718-112718

    • DOI

      10.1016/j.jphotobiol.2023.112718

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Formation of a stable PSI-PSII megacomplex in rice that conducts energy spillover2023

    • Author(s)
      Kim Eunchul、Yokono Makio、Tsugane Kazuo、Ishii Asako、Noda Chiyo、Minagawa Jun
    • Journal Title

      Plant And Cell Physiology

      Volume: - Issue: 8 Pages: 858-865

    • DOI

      10.1093/pcp/pcad037

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Conformation of Light-Harvesting Complex II Trimer Depends upon Its Binding Site2022

    • Author(s)
      Kim E, Kubota-Kawai H, Kawai F, Yokono M, Minagawa J
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 126 Issue: 31 Pages: 5855-5865

    • DOI

      10.1021/acs.jpcb.2c04061

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Editorial: Regulation of Light-Harvesting Systems During Acclimation of Photosynthetic Organisms2022

    • Author(s)
      Thomas Roach, Eunchul Kim, Lijin Tian, Bernard Lepetit
    • Journal Title

      Frontiers in Plant Science

      Volume: 13

    • DOI

      10.3389/fpls.2022.914047

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Plant and algal PSII-LHCII supercomplexes: structure, evolution and energy transfer.2021

    • Author(s)
      Sheng, X., Liu, Z., Kim, E., Minagawa, J.
    • Journal Title

      Plant Cell Physiol

      Volume: 62 Issue: 7 Pages: 1108-1120

    • DOI

      10.1093/pcp/pcab072

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Regulation of light-harvesting systems in plants: Flexible conformation of light-harvesting complex II trimer depending on its binding site2023

    • Author(s)
      Eunchul Kim, Hisako Kubota-Kawai, Fumihiro Kawai, Makio Yokono and Jun Minagawa
    • Organizer
      67th Biophysical Society Annual Meeting
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation on the Function and Regulatory Factor of Multimeric PSII Supercomplexes2022

    • Author(s)
      Eunchul Kim, Asako Ishii, Chiyo Noda, Jun Minagawa
    • Organizer
      International Congress on Photosynthesis Research 2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] タンパク質間相互作用による光合成集光システムの調節2022

    • Author(s)
      Eunchul Kim, Jun Minagawa
    • Organizer
      第60回日本生物物理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Heterogeneity of photosystem megacomplexes in green plants2022

    • Author(s)
      Eunchul Kim, Jun Minagawa
    • Organizer
      International Symposium on Photosynthesis and Chloroplast Regulation
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 緑藻クラミドモナスにおける LHCII サブユニットの光防御機能解析2022

    • Author(s)
      Eunchul Kim、川上 恵典、鎌田 このみ、石井 麻子、皆川 純
    • Organizer
      第15回クラミドモナス研究会
    • Related Report
      2021 Research-status Report
  • [Presentation] Regulation of light-harvesting systems by inter-protein interactions in green algae and plants2021

    • Author(s)
      Eunchul Kim, Akimasa Watanabe, Keisuke Kawakami, Ryoichi Sato, Asako Ishii, Christopher D. P. Duffy, Alexander V. Ruban, Jun Minagawa
    • Organizer
      The 11th Asian Photochemistry Conference
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research

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Published: 2021-04-28   Modified: 2024-01-30  

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