| Project/Area Number |
21K17199
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 57080:Social dentistry-related
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
Oda Yuki 広島大学, 病院(歯), 専門研究員 (40641949)
|
|---|
| Project Period (FY) |
2021-04-01 – 2024-03-31
|
| Project Status |
Completed (Fiscal Year 2023)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2023: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2022: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | Autistic spectrum / oral flora / intestinal flora / ゲノム解析 / 16S rRNA gene analysis / oral microbiota / intestinal microbiota / next-generation sequence / プロバイオティクス / 障害者 / 自閉スペクトラム症 / L8020 / 腸脳相関 / 口腔内細菌 / 腸内細菌 / 口腔内細菌叢 |
|---|
| Outline of Research at the Start |
腸内細菌と脳機能や精神疾患が関連する「脳腸相関 brain-gut interaction」についてエビデンスが蓄積されており，精神疾患のひとつである自閉スペクトラム症では腸内細菌叢とその重症度が関連することが報告されている．近年，口腔内と腸内の細菌叢の関連が報告され，口腔・腸・脳相関の存在の可能性が高まっているが，その実態は明らかでない．本研究では，プロバイオティクスで自閉スぺクトラム症者の口腔内細菌叢を変化させ，腸内細菌叢およびその症状の変化を分析することで，口腔・腸・脳相関の実態を解明したい．
|
| Outline of Final Research Achievements |
In the first year, due to COVID-19, the number of patients with ASD visiting the hospital dropped sharply, making it difficult to determine the subjects. The study broadly targeted people with intellectual disabilities, and first examined whether there were differences in the oral flora of healthy people and those with intellectual disabilities. It was found that the diversity of the oral flora of people with intellectual disabilities was significantly lower than that of healthy people. The above results were presented at the Japanese Society of Disability Dentistry and the International Society of Disability Dentistry, and then compiled as an English paper. From the following year onwards, saliva, dental plaque, and stool were collected from 16 people with ASD and their siblings living together, and genome analysis was performed. It was found that the ASD group had significantly higher diversity in saliva and dental plaque than healthy people, but there was no difference in stool.
|
| Academic Significance and Societal Importance of the Research Achievements |
本研究の成果より、自閉スペクトラム症者の細菌叢を健常者と比較した結果、唾液と歯垢では自閉症者で多様性が有意に高かった。一方で、かねてより報告されていた便においては多様性に違いがみられなかった。かねてより、精神疾患の精神症状と腸内細菌叢に何らかの関連がある可能性が指摘されているが、本研究よりASD群と健常群の口腔内細菌叢の違いは、腸内細菌叢における違いよりも顕著であった。このことから、口腔・腸・脳相関が存在する可能性が示唆された。
|
