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Development of high-sensitivity time-resolved CD measuring system for elucidation of functional biopolymers dynamics

Research Project

Project/Area Number 21K18992
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 35:Polymers, organic materials, and related fields
Research InstitutionTohoku University

Principal Investigator

WADA Takehiko  東北大学, 多元物質科学研究所, 教授 (20220957)

Project Period (FY) 2021-07-09 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2022: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2021: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsCDスペクトル / 楕円偏光 / 方位角制御 / 円偏光二色性 / 高感度測定 / 三重項 / 過渡吸収 / 高時間分解 / 円二色性スペクトル測定 / 高感度 / 楕円偏光検出 / 時間分解 / 円二色性 / 構造変化検出 / 動的挙動解析
Outline of Research at the Start

タンパク質や核酸の複合体形成過程の検出と解析は、基礎科学的観点ならびに新規高効率生体機能性分子開発の観点からも重要な研究テーマであり、高効率で精度の高い分子開発には、複合体形成過程の動的挙動の直接的観察と解析が極めて重要と考えられ円二色性(CD)スペクトル測定が実時間観測には有効な手法であると認識されてきた。しかし、現行CD測定の時間分解能は高感度測定手法の原理的限界に基づきミリ秒程度で、マイクロ秒程度と予想されている早い複合体形成過程の検出には、本質的に適用不可能であり、根本的な解決課題を有している。本研究では高感度と高時間分解能を両立可能な新規CD測定原理の提案と測定装置を目指す。

Outline of Final Research Achievements

Circular dichroism (CD) spectroscopy is widely used to detect structural changes in chiral molecular systems. However, the time resolution of current CD spectrometers is limited to m-sec order because of the utilization of the PEM. To observe more fast events of chiral molecules, it has been desired to develop a novel CD measurement system that can achieve both high sensitivity and high temporal resolution of micro-sec order.
In this study, we have developed a CD measurement system with high sensitivity and time resolution by utilizing a new detection method using elliptically polarized light, which was proposed by our collaborator Assoc. Prof. Yasuyuki Araki, and demonstrated that the high sensitivity and high time-resolution observation method based on azimuthal change works effectively. We also have demonstrated that the transient CD measurement method developed in this study can measure the CD spectra of excited triplet states of organic molecules in solution at room temperature.

Academic Significance and Societal Importance of the Research Achievements

左右の円偏光吸収の差異であるCDシグナルは、シグナル強度が小さいため高感度測定が困難である事も広く知られ、SMを用いることで本課題を解決しているが、時間分解能はミリ秒程度が上限である。このため高感度と高時間分解能を両立可能な新規CD測定原理の開発が待望されてきた。本研究では荒木准教授が提案した新規楕円偏光制御法に基づく高感度CD検出装置の構築に取り組み、方位角変化に基づく高感度・高時間分解観測法が有効に機能することを実証した。さらに本研究で構築した装置により、室温・溶液中で有機分子の励起三重項状態のCDスペクトルを測定可能であることを実証し、その波及効果は極めて大きいと評価されている。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (17 results)

All 2023 2022 2021 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 3 results) Presentation (8 results) (of which Int'l Joint Research: 8 results,  Invited: 6 results) Book (3 results)

  • [Int'l Joint Research] University de Bordeaux(フランス)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] University of Bordeaux(フランス)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Unique Crosslinking Properties of Psoralen‐conjugated Oligonucleotides developed by Novel Psoralen N‐Hydroxysuccinimide Esters2023

    • Author(s)
      Nakao Juki、Mikame Yu、Eshima Honoka、Yamamoto Tsuyoshi、Dohno Chikara、Wada Takehiko、Yamayoshi Asako
    • Journal Title

      ChemBioChem

      Volume: - Issue: 15 Pages: 1-9

    • DOI

      10.1002/cbic.202200789

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Selective Photo-Crosslinking Detection of Methylated Cytosine in DNA Duplex Aided by a Cationic Comb-Type Copolymer2022

    • Author(s)
      Kojima Atsuhiro、Nakao Juki、Shimada Naohiko、Yoshida Naoki、Abe Yota、Mikame Yu、Yamamoto Tsuyoshi、Wada Takehiko、Maruyama Atsushi、Yamayoshi Asako
    • Journal Title

      ACS Biomaterials Science & Engineering

      Volume: 8 Issue: 5 Pages: 1799-1805

    • DOI

      10.1021/acsbiomaterials.2c00048

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Amphiphilic Perforated Honeycomb Films for Gravimetric Liquid Separation2021

    • Author(s)
      Chen Bihai、Wada Takehiko、Yabu Hiroshi
    • Journal Title

      Advanced Materials Interfaces

      Volume: 9 Issue: 1 Pages: 2101954-2101954

    • DOI

      10.1002/admi.202101954

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Random laser oscillation from an organic fluorescent dye loaded inside a porous zirconia medium2021

    • Author(s)
      Sakurayama Yukari、Onodera Tsunenobu、Araki Yasuyuki、Wada Takehiko、Oikawa Hidetoshi
    • Journal Title

      RSC Advances

      Volume: 11 Issue: 51 Pages: 32030-32037

    • DOI

      10.1039/d1ra05791j

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] Novel Design Strategy of DNA-Artificial Nucleic Acid Chimera Toward Enhancement of Target RNA Cleavage Activities2022

    • Author(s)
      Takehiko WADA
    • Organizer
      8th Annual Data Science in Engineering and Life Sciences Symposium, Case Western Reserve University, Cleveland, USA
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Remarkable Enhancement of RNaseH Cleavage Activities of RNA Complexed with Chimeric DNA-Peptide Ribonucleic Acid (PRNA)2022

    • Author(s)
      Takehiko WADA
    • Organizer
      Oligonucleotide Therapeutics Society Symposium, Tokyo
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Design, Synthesis, and Properties of Functionalized Artificial Nucleic Acids: Toward Creation of Cancer Cell Selective Nucleic Acids Medicine2022

    • Author(s)
      Takehiko WADA
    • Organizer
      Melbourne University - Tohoku University Joint Work Shop, Melbourne, Australia
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Novel Design Strategy of DNA-Artificial Nucleic Acid Chimera Toward Enhancement of Target RNA Cleavage Activities:Application for COVID-19 Therapeutics2021

    • Author(s)
      Masahito Inagaki,Nozomu Ishiwata,Ryota Azuma,Masaki NIshijima,Hironori Hayashi,Yasuyuki Araki,Eiichi Kodama,Takehiko Wada
    • Organizer
      ISNAC2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Photochirogenesis of 2-Anthracene Carboxylate and 2,6-Anthracene Dicarboxylate mediated by Serum Albumin as Chiral Reaction Nanoreactor2021

    • Author(s)
      Masaki Nishijima, Nagisa Kanazawa, Misaki Kawai, Sota Toyooka, Yuta Fujishiro, Tadashi Mori, Yasuyuki Araki, Yoshihisa Inoue, ○Takehiko WADA
    • Organizer
      MRM2021(Materials Research Meeting)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] DNA-artificial nucleic acids chimeric engineering strategy for enhancement of RNA cleavage function utilizing RNaseH2021

    • Author(s)
      ○Takehiko WADA,
    • Organizer
      PACIFICHEM2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Novel design strategy of XNA for enhancement of target RNA cleavage activities with RNaseH and therapeutic effects2021

    • Author(s)
      ○Takehiko WADA, Presenter; Masahito Inagaki; Akira Yano; Tsuyoshi Yamamoto; Seiya ISHIZAWA; Satoru Ishibashi; Masaki NISHIJIMA; Yasuyuki ARAKI; Satoko Suzuki; Asako Yamayoshi; Takanori Yokota
    • Organizer
      PACIFICHEM2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Supramolecular enantio- and regioselective photocyclodimerization of 2-anthracenecarboxylate mediated by hybrid nanofibers and antibodies in water2021

    • Author(s)
      Takehiko WADA, Presenter; Wijak YOSPANYA; Masaki NISHIJIMA; Yasuyuki ARAKI; Reiko ODA
    • Organizer
      PACIFICHEM2021
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Book] 高分子材料の事典2022

    • Author(s)
      中條善樹、大山俊幸、和田健彦
    • Total Pages
      630
    • Publisher
      朝倉書店
    • ISBN
      9784254252729
    • Related Report
      2022 Annual Research Report
  • [Book] 刺激応答性高分子の開発動向2021

    • Author(s)
      荏原充宏
    • Total Pages
      315
    • Publisher
      シーエムシー出版
    • ISBN
      9784781316116
    • Related Report
      2021 Research-status Report
  • [Book] 進化を続ける核酸化学2021

    • Author(s)
      日本化学会
    • Total Pages
      228
    • Publisher
      化学同人
    • ISBN
      9784759814019
    • Related Report
      2021 Research-status Report

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Published: 2021-07-13   Modified: 2024-01-30  

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