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Metabolic recycling of plant hormone in organella.

Research Project

Project/Area Number 21K19097
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 38:Agricultural chemistry and related fields
Research InstitutionOkayama University of Science

Principal Investigator

Hayashi Ken-ichiro  岡山理科大学, 生命科学部, 教授 (30289136)

Project Period (FY) 2021-07-09 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2023: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2022: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2021: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsオーキシン / 植物ホルモン / 代謝 / 代謝不活性化 / オルガネラ / 貯蔵型ホルモン
Outline of Research at the Start

植物ホルモンであるオーキシンは,核内受容体に結合してホルモン活性を発揮する。細胞内のオーキシンが,小胞体などの細胞内オルガネラへ一時貯蔵されることよって,核へと移行するオーキシン量が調節されることを実験的に証明する。それにより,これまでのホルモンの生合成・輸送・分解によるホルモン活性の調節に加えて,ホルモンのオルガネラ局在制御による新奇な植物ホルモンの活性調節機構の解明に挑む。

Outline of Final Research Achievements

The intracellular auxin level is coordinately regulated by biosynthesis, transport, and metabolic inactivation of auxin. Endogenous auxin level is maintained by the expression and degradation rate of these metabolic enzymes. This study revealed that auxin storage forms are accumulated in intracellular organelles, which play an important role in maintaining auxin homeostasis to maintain auxin homeostasis. This study illustrates the physiological function of auxin storage forms.

Academic Significance and Societal Importance of the Research Achievements

オーキシンの細胞濃度の調節は,植物の分化成長の制御に直接関係することから,植物学上の重要な研究課題である。オーキシンは発根,茎の徒長,および結実のなどの農業上重要な形質にも関係する。オーキシンの配糖体やアミノ酸複合体などのオーキシン代謝物は,不活性化物として考えられてきたが,本研究によって,オーキシンの貯蔵体として小胞体などの細胞内コンパートメントへ一時貯蔵されることを見出した。これらオーキシンの合成・分解経路の全貌を解明することで,作物・園芸植物などの形質の理解につながると期待できる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (19 results)

All 2023 2022 Other

All Int'l Joint Research (7 results) Journal Article (6 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (6 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results)

  • [Int'l Joint Research] カルフォルニア大学サンディエゴ校(米国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] University of Leeds/University of Warwick(英国)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Ghent University(ベルギー)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Washington University/University of California San Diego(米国)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] University of Warwick(英国)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] University of California San Diego(米国)

    • Related Report
      2021 Research-status Report
  • [Int'l Joint Research] University of Warwick/University of Leeds(英国)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Intrinsic disorder and conformational coexistence in auxin coreceptors2023

    • Author(s)
      Sigurd Ramans-Harborough, Arnout P Kalverda, Iain W Manfield, Gary S Thompson, Martin Kieffer, Veselina Uzunova, Mussa Quareshy, Justyna M Prusinska, Suruchi Roychoudhry, Ken-Ichiro Hayashi, Richard Napier, Charo Del Genio, Stefan Kepinski
    • Journal Title

      Proc Natl Acad Sci U S A .

      Volume: 120 Issue: 40

    • DOI

      10.1073/pnas.2221286120

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] GH3-mediated auxin inactivation attenuates multiple stages of lateral root development2023

    • Author(s)
      Qing Wang, Hugues De Gernier, Xingliang Duan, Yuanming Xie, Danny Geelen, Ken-Ishiro Hayashi, Wei Xuan, Markus Geisler, Kirsten Ten Tusscher, Tom Beeckman, Steffen Vanneste
    • Journal Title

      New Phytologist

      Volume: 240 Issue: 5 Pages: 1900-1912

    • DOI

      10.1111/nph.19284

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] オーキシンの生合成と不活性化経路 (総説)2023

    • Author(s)
      林 謙一郎、笠原博幸
    • Journal Title

      植物の生長調節

      Volume: 58 Pages: 8-18

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Auxin Regulates Apical Stem Cell Regeneration and Tip Growth in the Marine Red Alga Neopyropia yezoensis2022

    • Author(s)
      Taya K, Takeuchi S, Takahashi M, Hayashi KI, Mikami K.
    • Journal Title

      Cells

      Volume: 11 Issue: 17 Pages: 2652-2652

    • DOI

      10.3390/cells11172652

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Chemical inhibition of the auxin inactivation pathway uncovers the roles of metabolic turnover in auxin homeostasis2022

    • Author(s)
      Fukui K, Arai K, Tanaka Y, Aoi Y, Kukshal V, Jez JM, Kubes MF, Napier R, Zhao Y, Kasahara H, Hayashi KI.
    • Journal Title

      Proc Natl Acad Sci U S A

      Volume: 119 Issue: 32

    • DOI

      10.1073/pnas.2206869119

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The auxin-inducible degron 2 (AID2) system enables controlled protein knockdown during embryogenesis and development in Caenorhabditis elegans2022

    • Author(s)
      Negishi T, Kitagawa S, Horii N, Tanaka Y, Haruta N, Sugimoto A, Sawa H, Hayashi KI, Harata M, Kanemaki MT.
    • Journal Title

      Genetics

      Volume: 220 Issue: 2

    • DOI

      10.1093/genetics/iyab218

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Auxin homeostasis is coordinately regulated by GH3 and AUX12023

    • Author(s)
      Ken-ichiro Hayashi*, Kazushi Arai, Masaaki Watahiki, Richard Napier, Yunde Zhao, Kosuke Fukui, Hiroyuki Kasahara
    • Organizer
      The 24th International Conference on. Plant Growth Substances.
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] オーキシンホメオスタシスにおけるUGT76F1配糖体化酵素の機能2023

    • Author(s)
      原田 実緒 、丸山 海成、Yunde Zhao、笠原 博幸、林 謙一郎
    • Organizer
      植物化学調節学会 第58回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] IAA不活性化酵素の細胞内局在解析2023

    • Author(s)
      窪津 智彬、丸山 海成、Yunde Zhao、笠原 博幸、林 謙一郎
    • Organizer
      植物化学調節学会 第58回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 胞子植物におけるオーキシン不活化経路の解析2022

    • Author(s)
      菅沼 有紀、丸山 海成、嶋村 正樹、林 謙一郎、笠原 博幸
    • Organizer
      植物化学調節学会
    • Related Report
      2022 Research-status Report
  • [Presentation] Chemical Biology of Indole Derivatives from Plant Hormone to Medicine2022

    • Author(s)
      Ken-ichiro Hayashi
    • Organizer
      The 37th Symposium on Natural Products
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 田中佑佳,江田 智彬,前田 雅志,古川 毅,福井康祐,三井亮司,林謙一郎2022

    • Author(s)
      標的細胞・オルガネラへの代謝活性化を利用したオーキシンデリバリー制御系の構築
    • Organizer
      日本農芸化学会中四国支部第61回講演会(例会)
    • Related Report
      2021 Research-status Report

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Published: 2021-07-13   Modified: 2025-01-30  

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