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Development of liposome-typed molecular robots that will work in air and sea water

Research Project

Project/Area Number 21K19786
Research Category

Grant-in-Aid for Challenging Research (Exploratory)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 61:Human informatics and related fields
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

Kawano Ryuji  東京農工大学, 工学(系)研究科(研究院), 教授 (90401702)

Co-Investigator(Kenkyū-buntansha) 小祝 敬一郎  東京海洋大学, 学術研究院, 助教 (10867617)
Project Period (FY) 2021-07-09 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2022: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2021: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Keywords脂質二分子膜 / リポソーム / マイクロ流路 / 分子ロボット / シャボン玉 / マイクロ流体 / 海水
Outline of Research at the Start

本研究では1)海水中で動作するリポソーム型分子ロボット、2)空気中で動作するシャボン玉型分子ロボットのプロトタイプ開発を行う。これらが開発できた場合、実用的には、例えば水産養殖現場での環境モニタリングや病原性微生物モニタリング・浄化、大気汚染のモニタリング・浄化(PM2.5の捕捉)への応用展開が期待できる。

Outline of Final Research Achievements

The researchers aimed to develop cell-sized molecular robots that can operate in natural and dehydrated environments. Liposome and soap bubble types were attempted using PDMS flow and glass capillary channels. Stable liposomes were produced by optimizing the ratio of interfacial tension, while soap bubbles were produced using a channel with air and soap bubble liquid. Hydrodynamic simulations are underway to create micro-sized soap bubbles. The findings have shown promising results, but challenges remain such as improving the stability of liposomes in seawater and fabricating smaller soap bubbles.

Academic Significance and Societal Importance of the Research Achievements

分子ロボットは、分子でできた部品をアセンブルして任意の仕事を行う細胞サイズのロボットと定義された次世代の生体模倣型ロボットである。リポソーム型分子ロボットは脂質二分子膜でできたカプセルで、脂質膜中に膜タンパク質や疎水性分子、内部に水溶性の様々な機能性分子を内包でき高度な機能の埋め込みが可能であることから、申請者はこのリポソーム型の分子ロボットの開発に取り組んでいる。これまで分子ロボットの研究のほとんどが生体内や単純バッファー中での検討しか行われていなため自然環境中や大気のような脱水環境での使用が望まれると考え、本研究を推進し、海水中・大気中で作動可能な分子ロボットのプロトタイプの開発を行った。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (15 results)

All 2023 2022 2021 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (6 results) (of which Int'l Joint Research: 4 results,  Invited: 1 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Liposome Deformation Induced by Membrane-Binding Peptides2023

    • Author(s)
      Izumi Kayano, Saito Chihiro, Kawano Ryuji
    • Journal Title

      Micromachines

      Volume: 14 Issue: 2 Pages: 373-373

    • DOI

      10.3390/mi14020373

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Long-term survivable liposomes in seawater2023

    • Author(s)
      Izumi Kayano, Keiichiro Koiwai, Kawano Ryuji
    • Journal Title

      Biophysical Journal

      Volume: 122 Issue: 3 Pages: 82a-82a

    • DOI

      10.1016/j.bpj.2022.11.650

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Toward Operation of Molecular Robot in the Air: Glass Capillary-based Generation of Reprosducible Soap Bubble2022

    • Author(s)
      Rina Takagi, Sotaro Takiguchi, Ryuji Kawano
    • Journal Title

      Proceedings of MicroTAS

      Volume: 1 Pages: 74-75

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Liposome Deformation Stimulated by Peptides2022

    • Author(s)
      Izumi Kayano, Kawano Ryuji
    • Journal Title

      Proceedings of MicroTAS

      Volume: 1 Pages: 369-370

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 分子でつくるロボットーリポソーム型分子ロボットの開発2021

    • Author(s)
      小祝敬一郎、和泉佳弥乃、川野竜司
    • Journal Title

      化学

      Volume: 76 Pages: 70-71

    • Related Report
      2021 Research-status Report
  • [Presentation] Long-term survivable liposomes in seawater2023

    • Author(s)
      Izumi Kayano, Keiichiro Koiwai, Kawano Ryuji
    • Organizer
      BPS2023
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 人工細胞膜システムを創る2022

    • Author(s)
      川野竜司
    • Organizer
      細胞を創る研究会15.0
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Toward Operation of Molecular Robot in the Air: Glass Capillary-based Generation of Reprosducible Soap Bubble2022

    • Author(s)
      Rina Takagi, Sotaro Takiguchi, Ryuji Kawano
    • Organizer
      uTAS2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Liposome Deformation Stimulated by Peptides2022

    • Author(s)
      Izumi Kayano, Kawano Ryuji
    • Organizer
      uTAS2022
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Rupture-reseal liposomes generated by microfluidic device2022

    • Author(s)
      JiaJue Ji, Kayano Izumi, Ryuji Kawano
    • Organizer
      CHEMINAS46
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ガラスキャピラリーによる均一サイズリポソームの作製2021

    • Author(s)
      高木里菜,滝口創太郎,小祝敬一郎,鈴木宏明,川野竜司
    • Organizer
      化学とマイクロ・ナノシステム学会 第44 回研究会
    • Related Report
      2021 Research-status Report
  • [Book] Molecular Robotics An Introduction2022

    • Author(s)
      Satoshi Murata
    • Total Pages
      296
    • Publisher
      Springer Nature
    • ISBN
      9789811939877
    • Related Report
      2022 Annual Research Report
  • [Book] Cell-Penetrating Peptides: Design, Development and Applications2022

    • Author(s)
      Makoto Oba, Yosuke Demizu
    • Total Pages
      416
    • Publisher
      Wiley&Sons
    • ISBN
      9783527350117
    • Related Report
      2022 Annual Research Report
  • [Remarks] 東京農工大学 川野研究室

    • URL

      http://web.tuat.ac.jp/~rjkawano/index.html

    • Related Report
      2022 Annual Research Report
  • [Remarks] 東京農工大学 川野研究室

    • URL

      http://web.tuat.ac.jp/~rjkawano/index.html

    • Related Report
      2021 Research-status Report

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Published: 2021-07-13   Modified: 2024-01-30  

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