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Analysis of the ancestral function of the plant hormone KL as a regulator of vegetative reproduction using hornworts

Research Project

Project/Area Number 21K20649
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeMulti-year Fund
Review Section 0702:Biology at cellular to organismal levels, and related fields
Research InstitutionTohoku University

Principal Investigator

Hidemasa Suzuki  東北大学, 生命科学研究科, 助教 (30908059)

Project Period (FY) 2021-08-30 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords陸上植物 / 進化 / 栄養繁殖 / 植物ホルモン / KL信号伝達 / コケ植物 / ツノゴケ類 / 植物 / ツノゴケ / 頂端幹細胞 / 発生 / KAI2-Ligand (KL) / 植物ホルモンKL
Outline of Research at the Start

本研究は、陸上植物の栄養繁殖に普遍的なしくみの解明を目指す。植物ホルモンKAI2-Ligand (KL)は栄養繁殖の制御因子として誕生したという仮説のもと、陸上植物進化の最早期に分岐したコケ植物の祖先種でそのしくみが確立されていたのかを明らかにする。そのために、本研究ではコケ植物の中でも基部に位置するツノゴケ類ホウライツノゴケの実験系を整備する。系統的に離れたホウライツノゴケと苔類ゼニゴケで、KL経路とKL経路の標的遺伝子がともに栄養繁殖を調節していることを分子遺伝学の手法で検証する。

Outline of Final Research Achievements

Plants propagate vigorously by generating clones through a process called vegetative reproduction. KAI2-Ligand (KL), an as-yet-unknown novel plant hormone, is one of the regulators of vegetative propagation. In this study, we investigated the mechanism of KL signaling and vegetative reproduction in hornworts.
In the hornwort Anthoceros agrestis, we comprehensively searched for target genes of KL signaling, and identified genes that are widely conserved in land plants. Additionally, we generated KL-response reporter plants and plants that blocked the KL signaling to further analyze the physiological function of KL. Furthermore, we explored the developmental basis of vegetative reproduction in the hornwort A. angustus, which evolved a unique vegetative reproduction system. To facilitate future experiments as a new research model for vegetative reproduction, we established a transformation method in A. angustus.

Academic Significance and Societal Importance of the Research Achievements

栄養繁殖は植物の重要な繁殖戦略であるとともに、植物栽培における重要な性質でもある。本研究では、陸上植物で共通してKL信号伝達に制御される遺伝子を明らかにした。これらの遺伝子とKLによる栄養繁殖制御の関係を明らかにすることで、栄養繁殖を精緻に制御する技術につながると期待される。
また、本研究ではホウライツノゴケという新たな栄養繁殖の研究モデルを立ち上げた。ツノゴケ類はコケ植物のなかでも最初期に分岐した系統であり、被子植物などの維管束植物や従来のモデルコケ植物とは進化的に大きな隔たりがある。今後、栄養繁殖の普遍性や多様性を解明する研究への展開が期待される。

Report

(3 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • Research Products

    (18 results)

All 2023 2022 2021

All Journal Article (4 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (14 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] コケ植物の栄養繁殖の分子基盤解明に向けて2023

    • Author(s)
      鈴木秀政
    • Journal Title

      アグリバイオ

      Volume: 7 Pages: 64-67

    • Related Report
      2022 Annual Research Report
  • [Journal Article] Auxin signaling is essential for organogenesis but not for cell survival in the liverwort<i>Marchantia polymorpha</i>2022

    • Author(s)
      Suzuki Hidemasa、Kato Hirotaka、Iwano Megumi、Nishihama Ryuichi、Kohchi Takayuki
    • Journal Title

      The Plant Cell

      Volume: 35 Issue: 3 Pages: 1058-1075

    • DOI

      10.1093/plcell/koac367

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diminished Auxin Signaling Triggers Cellular Reprogramming by Inducing a Regeneration Factor in the Liverwort <i>Marchantia polymorpha</i>2022

    • Author(s)
      Ishida Sakiko、Suzuki Hidemasa、Iwaki Aya、Kawamura Shogo、Yamaoka Shohei、Kojima Mikiko、Takebayashi Yumiko、Yamaguchi Katsushi、Shigenobu Shuji、Sakakibara Hitoshi、Kohchi Takayuki、Nishihama Ryuichi
    • Journal Title

      Plant and Cell Physiology

      Volume: 63 Issue: 3 Pages: 384-400

    • DOI

      10.1093/pcp/pcac004

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] An Ancestral Function of Strigolactones as Symbiotic Rhizosphere Signals2021

    • Author(s)
      Kodama Kyoichi、Rich Melanie K.、Yoda Akiyoshi、Shimazaki Shota、Xie Xiaonan、Akiyama Kohki、--- 、Yamaguchi Shinjiro、Shimamura Masaki、Delaux Pierre-Marc、Nomura Takahito、Kyozuka Junko
    • Journal Title

      BioRxv

      Volume: -

    • DOI

      10.1101/2021.08.20.457034

    • Related Report
      2021 Research-status Report
    • Open Access / Int'l Joint Research
  • [Presentation] Analysis of vegetative reproduction in the hornwort Anthoceros angustus2023

    • Author(s)
      Hidemasa Suzuki, Junko Kyozuka
    • Organizer
      日本植物生理学会第64回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゼニゴケにおけるKL信号伝達系の下流因子探索2023

    • Author(s)
      Kazato Kumagai, Hidemasa Suzuki, Aino Komatsu, Kyoichi Kodama, Xie X, Junko Kyozuka
    • Organizer
      日本植物生理学会第64回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Cytokinin works downstream of the KL signaling pathway to control vegetative reproduction in Marchantia polymorpha.2023

    • Author(s)
      Aino Komatsu, Mizuki Fujibayashi, Fukutaro Hosoya, Kazato Kumagai, Hidemasa Suzuki, Kyoichi Kodama, Yohei Mizuno, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara, Xiaonan Xie, Satoshi Naramoto, Junko Kyozuka
    • Organizer
      日本植物生理学会第64回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Roles and downstream gene networks of Rboh-mediated ROS production in cell division pattern and cell cycle progression in Marchantia polymorpha2023

    • Author(s)
      Yuto Yamashita, Yuki Hagiwara, Kenji Hashimoto, Hidemasa Suzuki, Ryuichi Nishihama, Kazuyuki Kuchitsu
    • Organizer
      日本植物生理学会第64回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] サイトカイニンによるタイ類ゼニゴケの栄養繁殖制御2022

    • Author(s)
      小松 愛乃, 藤林 瑞季, 細矢 福多郎, 熊谷 風杜, 鈴木 秀政, 児玉 恭一, 竹林 裕美子, 小嶋 美紀子, 榊原 均, 経塚 淳子
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ツノゴケ類ホウライツノゴケにおける栄養繁殖の遺伝的制御2022

    • Author(s)
      鈴木秀政,経塚淳子
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 苔類ゼニゴケにおけるKL信号伝達の下流因子探索2022

    • Author(s)
      熊谷風杜、鈴木秀政、小松愛乃、児玉恭一、経塚淳子
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Analysis of the vegetative reproduction in the hornwort Anthoceros angustus2022

    • Author(s)
      Hidemasa Suzuki, Junko Kyozuka
    • Organizer
      EMBO Worksop: An integrated view of early land plant evolution
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Low auxin responsiveness, key to cellular reprogramming and stem cell regulation in Marchantia polymorpha2022

    • Author(s)
      Ryuichi Nishihama, Sakiko Ishida, Hidemasa Suzuki, Takayuki Kohchi
    • Organizer
      EMBO Worksop: An integrated view of early land plant evolution
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ゼニゴケのMpRbohsによる活性酸素種(ROS)生成の細胞分裂・分化における役割と標的因子の探索2022

    • Author(s)
      山下 優音,萩原 雄樹,橋本 研志,鈴木 秀政,西浜 竜一,朽津 和幸
    • Organizer
      日本植物学会第86回大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Analysis of the vegetative reproduction in the hornwort Anthoceros angustus2022

    • Author(s)
      Hidemasa Suzuki, Junko Kyozuka
    • Organizer
      The 63rd Annual Meeting of the Japanese Society of Plant Physiologists
    • Related Report
      2021 Research-status Report
  • [Presentation] Control of vegetative reproduction by KL signaling in Marchantia polymorpha2022

    • Author(s)
      Junko Kyozuka, Aino Komatsu, Kyoichi Kodama, Kazato Kumagai, Hidemasa Suzuki
    • Organizer
      The 63rd Annual Meeting of the Japanese Society of Plant Physiologists
    • Related Report
      2021 Research-status Report
  • [Presentation] ホウライツノゴケの栄養成長2022

    • Author(s)
      鈴木秀政、経塚淳子
    • Organizer
      第4 回コケ幹細胞研究
    • Related Report
      2021 Research-status Report
  • [Presentation] Transcriptome approach to reveal target genes of SMXL in the liverwort Marchantia polymorpha2021

    • Author(s)
      Hidemasa Suzuki, Kazato Kumagai, Aino Komatsu, Kyoichi Kodama, Junko Kyozuka
    • Organizer
      3rd International Congress on Strigolacones.
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research

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Published: 2021-10-22   Modified: 2024-01-30  

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