| Budget Amount *help |
¥217,490,000 (Direct Cost: ¥167,300,000、Indirect Cost: ¥50,190,000)
Fiscal Year 2014: ¥34,580,000 (Direct Cost: ¥26,600,000、Indirect Cost: ¥7,980,000)
Fiscal Year 2013: ¥34,580,000 (Direct Cost: ¥26,600,000、Indirect Cost: ¥7,980,000)
Fiscal Year 2012: ¥34,580,000 (Direct Cost: ¥26,600,000、Indirect Cost: ¥7,980,000)
Fiscal Year 2011: ¥48,360,000 (Direct Cost: ¥37,200,000、Indirect Cost: ¥11,160,000)
Fiscal Year 2010: ¥65,390,000 (Direct Cost: ¥50,300,000、Indirect Cost: ¥15,090,000)
|
|---|
| Outline of Final Research Achievements |
This project aims to study regulatory gene expression associated with various biological processes mediated by post-transcriptional modifications. We established a biochemical method called "inosine chemical erasing (ICE)" to directly identify inosines on RNA strands with high reliability, and applied the ICE method combined with deep sequencing (ICE-seq) to successfully map about 20,000 novel A-to-I RNA editing sites in human brain transcriptome. As novel RNA modifications, we identified 2-agmatinylcytidine (agm2C) and cyclic t6A (ct6A) from tRNAs, and studies their function and biogenesis.
|
