Elucidation of the mammalian mitochondrial respiration mechanism at the atomic level.

• Project/Area Number: 22247012
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: University of Hyogo
• Principal Investigator: YOSHIKAWA Shinya 兵庫県立大学, 生命理学研究科, 教授 (40068119)
• Co-Investigator(Renkei-kenkyūsha): OGURA Takashi 兵庫県立大学, 大学院・生命理学研究科, 教授 (70183770) ; SHIMADA Hideo 兵庫県立大学, 大学院・生命理学研究科, 教授 (80095611) ; MIYAZAWA Otsuo 兵庫県立大学, 大学院・生命理学研究科, 教授 (60247252) ; MURAMOTO Kazumasa 兵庫県立大学, 大学院・生命理学研究科, 准教授 (50305679) ; KAMIYA Katsumasa 筑波大学, 大学院・数理物質系, 助教 (60436243)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000); Fiscal Year 2012: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000); Fiscal Year 2011: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000); Fiscal Year 2010: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000)
• Keywords: 生体エネルギー変換 / 膜タンパク質 / 金属タンパク質 / X線結晶構造解析 / 振動分光解析

Research Abstract

X-ray structure of bovine heart cytochrome oxidase at the hydrogen atom level showed that the proton-pumping pathway (H-pathway) can accept 4 protons, while the time-resolved infrared analysis showed that the open/closed transition of H-pathway is controlled by the O2 reduction site. The mechanism of suppression of reactive oxygen production by NADH-ubiquinone reductase has been proposed vibrational spectroscopically. The 2D crystallization conditions for FoF1ATP synthase has been essentially established. The induction of conformationl changes in the transmembrane region of CcO by cytocrome c was discovered during the 2D crystallization of the complex of the two proteins.

Research Products

• [Journal Article] Resonance Raman Spectral properties of FMN of bovine Heart NADH:ubiquinone Oxidoreductase Suggesting a Mechanism for the Prevention of spontaneous Production of reactive Oxygen Species (2013)
  • Author(s): M. Hikita, K. Shinzawa-Itoh, M. Moriyama, T. Ogura, K. Kihira, S. Yoshikawa
  • Journal Title: Biochemistry Volume: 52 Pages: 98-104
• [Journal Article] EPR detection of two protein-associated ubiquinone components (SQNf and SQNs) in the membrane in situ and in proteoliposomes of isolated bovine heart complex I (2012)
  • Author(s): Tomoko Ohnishi, S. Tsuyoshi Ohnishi, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Ralph T. Weber
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume: 1817 Pages: 1803-1809
• [Journal Article] Two tyrosyl radicals stabilize high oxidation states in cytochrome c oxidase for efficient energy conservation and proton translocation (2012)
  • Author(s): Michelle A. Yu, Tsuyoshi Egawa, Kyoko Shinzawa-Itoh, Shinya Yoshikawa, Victor Guallar, Syun-Ru Yeh, Denis L. Rousseau, Gary J. Gerfen
  • Journal Title: Journal of the American Chemical Society Volume: 134 Pages: 4753-4761
  • Peer Reviewed
• [Journal Article] An intermediate conformational state during ligand binding to cytochrome c oxidase detected by time-resolved resonance Raman analyses of heme peripheral groups. Izumi Ishigami (2012)
  • Author(s): Takeshi Nishigaki, Kyoko Shinzawa-Itoh, Shinya Yoshikawa Takashi Ogura
  • Journal Title: Chemistry Letters Volume: 41 Pages: 178-180
  • Peer Reviewed
• [Journal Article] Reaction mechanism of Mammalian mitochondrial cytochrome C oxidase (2012)
  • Author(s): Shinya Yoshikawa, Kazumasa Muramoto, Kyoko Shinzawa-Itoh
  • Journal Title: Advances in experimental medicine and biology Volume: 748 Pages: 215-236
  • Peer Reviewed
• [Journal Article] EPR detection of two protein-associated ubiquinone components (SQNf and SQNs) in the membrane in situ and in proteoliposomes of isolated bovine heart complex I (2012)
  • Author(s): Tomoko Ohnishi
  • Journal Title: Biochimica et Biophysica Acta (BBA) ; Bioenergetics Volume: 1817 Issue: 10 Pages: 1803-1809
  • DOI: 10.1016/j.bbabio.2012.03.032
  • Peer Reviewed
• [Journal Article] Structural studies on bovine heart cytochrome c oxidase (2012)
  • Author(s): 吉川信也
  • Journal Title: Biochim.Biophys.Acta Volume: 1817 Issue: 4 Pages: 579-589
  • DOI: 10.1016/j.bbabio.2011.12.012
  • Peer Reviewed
• [Journal Article] An intermediate conformational state during ligand binding to cytochrome c oxidase detected by time-resolved resonance Raman analysis of heme peripheral groups (2012)
  • Author(s): Izumi Ishigami
  • Journal Title: Chem.Lett. Volume: 41 Pages: 178-180
  • Peer Reviewed
• [Journal Article] Two tyrosyl radicals stabilize high oxidation states in cytochrome c oxidase for efficient energy conservation and proton translocation (2012)
  • Author(s): Michelle Yu
  • Journal Title: J.Am.Chem.Soc. Volume: 1817 Issue: 10 Pages: 579-589
  • DOI: 10.1021/ja210535w
  • Peer Reviewed
• [Journal Article] Distinguishing between Cl^- and O_2^2- as the bridging element between Fe^3+ and Cu^2+ in resting-oxidized cytochrome c oxidase. Michihiro Suga (2011)
  • Author(s): Michihiro Suga,Naomine Yano, Kazumasa Muramoto, Kyoko Shinzawa-Itoh, Tomoko Maeda, Eiki Yamashita, Tomitake Tsukihara, Shinya Yoshikawa
  • Journal Title: Acta crystallographica. Section D Volume: 67 Pages: 742-744
  • Peer Reviewed
• [Journal Article] NMR basis for interprotein electron transfer gating between cytochrome c and cytochrome c oxidase (2011)
  • Author(s): Koichi Sakamoto, Masakatsu Kamiya, Mizue Imai, Kyoko Shinzawa-Itoh,Takeshi Uchida, Keiichi Kawano, Shinya Yoshikawa, Koichiro Ishimori
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 Pages: 12271-12276
  • Peer Reviewed
• [Journal Article] The O_2 reduction and proton pumping gate mechanism of bovine heart cytochrome c oxidase (2011)
  • Author(s): Shinya Yoshikawa, Kazumasa Muramoto, Kyoko Shinzawa-Itoh
  • Journal Title: Biochimica et biophysica acta Volume: 1807 Pages: 1279-1286
  • Peer Reviewed
• [Journal Article] Proton-pumping mechanism of cytochrome c oxidase (2011)
  • Author(s): Shinya Yoshikawa, Kazumasa Muramoto, Kyoko Shinzawa-Itoh
  • Journal Title: Annual Review of Biophysics Volume: 40 Pages: 205-223
  • Peer Reviewed
• [Journal Article] The O_2 reduction and proton pumping gate mechanism of bovine heart cytochrome c oxidase (2011)
  • Author(s): 吉川信也
  • Journal Title: Biochim.Biophys.Acta Volume: 1807 Issue: 10 Pages: 1279-1286
  • DOI: 10.1016/j.bbabio.2011.06.008
  • Peer Reviewed
• [Journal Article] NMR basis for interprotein electron transfer gating between cytochrome c and cytochrome c oxidase (2011)
  • Author(s): Koichi Sakamoto
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 108 Issue: 30 Pages: 12271-12276
  • DOI: 10.1073/pnas.1108320108
  • Peer Reviewed
• [Journal Article] Proton-pumping mechanism of of cytochrome c oxidase (2011)
  • Author(s): 吉川信也
  • Journal Title: Annu.Rev.Biophys. Volume: 40 Issue: 1 Pages: 205-223
  • DOI: 10.1146/annurev-biophys-042910-155341
  • Peer Reviewed
• [Journal Article] Distinguishing between Cl and O_2^<2-> as the bridging element between Fe^<3+> and Cu<2+> in the resting oxidized cytochrome c oxidase (2011)
  • Author(s): Michihiro Suga
  • Journal Title: Acta Cryst.D Volume: 67 Issue: 8 Pages: 742-744
  • DOI: 10.1107/s0907444911022803
  • Peer Reviewed
• [Journal Article] Bovine cytochrome c oxidase structures enable O_2 reduction with minimization of reactive oxygens and provide a proton-pumping gate (2010)
  • Author(s): Kazumasa Muramoto, Kazuhiro Ohta, Kyoko Shinzawa-Itoh, Katsumasa Kanda, Maki Taniguchi, Hiroyuki Nabekura, Eiki Yamashita, Tomitake Tsukihara, ShinyaYoshikawa
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 107 Pages: 7740-7745
  • Peer Reviewed
• [Journal Article] Cell-free synthesis of cytochrome c oxidase, a multicomponent membrane protein (2010)
  • Author(s): Yukie Katayama, Kunitoshi Shimokata, Makoto Suematsu, Takashi Ogura, Tomitake Tsukihara, Shinya Yoshikawa
  • Journal Title: Hideo Shimada Journal of Bioenergetics and Biomembranes Volume: 42 Pages: 235-240
  • Peer Reviewed
• [Journal Article] A resonance Raman band assignable to the O-O stretching mode in the resting oxidized state of bovine heart cytochrome c oxidase (2010)
  • Author(s): Miyuki Sakaguchi, Kyoko Shinzawa-Itoh, Shinya Yoshikawa
  • Journal Title: Takashi Ogura Journal of bioenergetics and biomembranes Volume: 42 Pages: 241-243
  • Peer Reviewed
• [Journal Article] X-ray structure of the NO-bound CuB in bovine cytochrome c oxidase (2010)
  • Author(s): Kazuhiro Ohta, Kazumasa Muramoto, Kyoko Shinzawa-Itoh, Eiki Yamashita, Shinya Yoshikawa
  • Journal Title: Tomitake Tsukihara Acta Crystallographica section F Volume: F66 Pages: 251-253
  • Peer Reviewed
• [Journal Article] Bovine cytochrome c oxidase structures enable O_2 reduction with minimization of active oxygen species and provide a proton pumping gate (2010)
  • Author(s): Kazumasa Muramoto
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 107 Pages: 7740-7745
  • Peer Reviewed
• [Journal Article] A Resonance Raman Band Assignable to the O-O Stretching Mode in the Resting Oxidized State of Bovine Heart Cytochrome c Oxidase (2010)
  • Author(s): Miyuki Sakaguchi
  • Journal Title: J.Bioenergetics and Biomembranes Volume: 42 Pages: 241-243
  • Peer Reviewed
• [Journal Article] Cell-free synthesis of cytochrome c oxidase, a multicomponent membrane protein (2010)
  • Author(s): Yukie Katayama
  • Journal Title: J.Bioenergetics and Biomembranes Volume: 42 Pages: 235-240
  • Peer Reviewed
• [Journal Article] X-ray structure of the NO-bound Cu(B) in bovine cytochrome c oxidase (2010)
  • Author(s): Kazuhiko Ohta
  • Journal Title: Acta Cryst.F Volume: 66 Pages: 251-253
  • Peer Reviewed
• [Journal Article] Bovine heart NADH-ubiquinone oxidoreductae contains one molecule of ubiquinone with ten isoprenoide units as one of the cofactors (2010)
  • Author(s): Kyoko Shinzawa-Itoh
  • Journal Title: Biochemistry Volume: 49 Pages: 487-492
  • Peer Reviewed
• [Presentation] Nanosecond time-resolved infrared basis for a bulge of the transmembrane helix between hemes a and a3 to facilitate highly efficient proton pumping by bovine heart cytochrome c oxidase (2012)
  • Author(s): Shinya Yoshikawa, Minoru Kubo, Satoru Nakashima, Satoru Yamaguchi, Takashi Ogura, Masao Mochizuki JiYoung Kang, Masaru Tateno, Kazumasa Muramoto
  • Organizer: Kyoko Shinzawa-Itoh Invited Lecture, European Bioenergetics Conference 2012, September 15th-20th Freiburg
  • Place of Presentation: Germany
• [Presentation] Structural studies on bovine heart cytochrome c oxidase (2012)
  • Author(s): Shinya Yoshikawa, Kazumasa Muramoto, Kyoko Shinzawa-Itoh
  • Organizer: Masao Mochizuki Biochimica et biophysica acta
• [Presentation] Reaction mmechanism of bovine heart cytochrome c oxidase (2012)
  • Author(s): Shinya Yoshikawa
  • Organizer: Invited Lecture, SBMPs (Structural Biology of Membrane Proteins) 4th Annual Meeting
  • Place of Presentation: Maratea, Italy
• [Presentation] The O_2 Reduction and Proton Pumping Mechanism Gate Mechanism of Bovine Heart Cytochrome c oxidase (2012)
  • Author(s): Shinya Yoshikawa
  • Organizer: Invited Lecture Workshop on Allosteric cooperativity in soluble, membrane bound hemoproteins and related membrane system
  • Place of Presentation: Bari Italy
• [Presentation] Nanosecond time-resolved infrared basis for a bulge of the transmembrane helix between hemes a and a3 to facilitate highly efficient proton pumping by bovine heart cytochrome c oxidase (2012)
  • Author(s): Shinya Yoshikawa
  • Organizer: European Bioenergetics Conference 2012
  • Place of Presentation: Freiburg (Germany)
  • Invited
• [Presentation] Reaction mmechanism of bovine heart cytochrome c oxidase (2012)
  • Author(s): Shinya Yoshikawa
  • Organizer: SBMPs (Structural Biology of Membrane Proteins) 4th Annual Meeting
  • Place of Presentation: Maratea (Italy)
  • Invited
• [Presentation] Mutational analyses of D-pathway of bovine heart cytochrome c oxidase suggest that the pathway does not transfer the pumping protons (2012)
  • Author(s): Ryota Aminaka
  • Organizer: European Bioenergetics Conference 2012
  • Place of Presentation: Freiburg (Germany)
• [Presentation] Development of Highly Sensitive Nanosecond Time-Resolved IR Apparatus Applicable to Protein System in H2O (2012)
  • Author(s): Satoru Nakashima
  • Organizer: European Bioenergetics Conference 2012
  • Place of Presentation: Freiburg （Germany）
• [Presentation] EPR analysis of two protein-associated ubiquinone (SQNf and SQNs) in the membrane in situ and in proteoliposomes of isolated bovine heart complex I (2012)
  • Author(s): S. Tsuyoshi Ohnishi
  • Organizer: European Bioenergetics Conference 2012
  • Place of Presentation: Freiburg （Germany）
• [Presentation] チトクロムcbb_3酸化酵素の発現と精製 (2011)
  • Author(s): 安藤友里子, 他
  • Organizer: 日本生体エネルギー研究会第37回討論会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2011-12-21
• [Presentation] チトクロム酸化酵素反応中間体 (2011)
  • Author(s): 木登梨佳, 他
  • Organizer: 日本生体エネルギー研究会第37回討論会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2011-12-21
• [Presentation] チトクロム酸化酵素反応サイクルの構造解析 (2011)
  • Author(s): 村本和優, 他
  • Organizer: 日本生体エネルギー研究会第37回討論会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2011-12-21
• [Presentation] Optimization of expression condition and purification of cbb_3-type cytochrome c oxidase (2011)
  • Author(s): 安藤友里子, 他
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学(兵庫県)
  • Year and Date: 2011-09-17
• [Presentation] Crystallization of bovine cytochrome c oxidase by new detergents (2011)
  • Author(s): 畑中美紀, 他
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] The effect of crystallization and cryoprotectant treatment on the absorption spectra of fully-oxidized cytochrome c oxidase (2011)
  • Author(s): 望月正雄, 他
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] X-ray structural analysis of cytochrome c oxidase in the resting oxidized state at 1.4Å resolution (2011)
  • Author(s): 矢野直峰, 他
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] X-ray structural analysis of R and O form intermediates of bovine cytochrome c oxidase (2011)
  • Author(s): 藤澤秀徳, 他
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] X-ray structural analysis of P form intermediate of bovine cytochrome c oxidase (2011)
  • Author(s): 木登梨佳, 他
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] X-ray structural analysis of F form intermediate of bovine cytochrome c oxidase (2011)
  • Author(s): 村本和優, 他
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] Structural changes of bovine cytochrome c oxidase dependent on the redox states (2011)
  • Author(s): 村本和優, 他
  • Organizer: XXII Congress and General Assembly of the Inter national Union of Crystallography
  • Place of Presentation: Madrid (Spain)
  • Year and Date: 2011-08-23
• [Presentation] High resolution analysis and anamolous dispersion analysis of bovine cytochrome c oxidase (2011)
  • Author(s): 矢野直峰, 他
  • Organizer: XXII Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: Madrid (Spain)
  • Year and Date: 2011-08-23
• [Presentation] High resolution structures of bovine cytochrome c oxidase in the resting oxidized and the reduced states (2011)
  • Author(s): 村本和優, 他
  • Organizer: Gordon Research Conferences, Bioenergetics
  • Place of Presentation: New Hampshire (USA)
  • Year and Date: 2011-06-27
• [Presentation] The O_2 reduction and protonpumping gate mechanisms of bovine heart cytochrome c oxidase (2011)
  • Author(s): 吉川信也, 他
  • Organizer: Gordon Research Conferences, Bioenergetics
  • Place of Presentation: New Hampshire (USA)
  • Year and Date: 2011-06-27
• [Presentation] ウシ心筋チトクロム酸化酵素還元型の1.4Å分解能X線構造解析 (2011)
  • Author(s): 藤澤秀徳, 他
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急(大阪府)
  • Year and Date: 2011-06-08
• [Presentation] ウシ心筋チトクロム酸化酵素休止酸化型の1.4Å分解能X線構造解析 (2011)
  • Author(s): 矢野直峰, 他
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急(大阪府)
  • Year and Date: 2011-06-08
• [Presentation] ウシ心筋チトクロム酸化酵素の還元・再酸化による構造変化 (2011)
  • Author(s): 村本和優, 他
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急(大阪府)
  • Year and Date: 2011-06-07
• [Presentation] Proton pumping mechanism of cytohrome c oxidase (2011)
  • Author(s): Shinya Yoshikawa
  • Organizer: Seminar series at Department of physiology and Biophysics, Albert Einstein College of Medicine
  • Place of Presentation: New York(Albert Einstein College of Medicine), USA
  • Year and Date: 2011-03-10
• [Presentation] Bovine cytohrome oxidase structures enable molecular oxygen reduction without formation of active oxygen species, providing a proton pumping gate. (2011)
  • Author(s): Shinya Yoshikawa
  • Organizer: 55^<th> Annual meeting of USA Biophysical Society
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2011-03-06
• [Presentation] The O_2 reduction and Proton Pumping Gate Mechanisms of Bovine Heart Cytochrome c Oxidase. (2011)
  • Author(s): Shinya Yoshikawa
  • Organizer: 欧州膜構造生物学協会(SBMPs)講演会
  • Place of Presentation: Grenoble(University Joseph Fourier), France
  • Year and Date: 2011-01-19
• [Presentation] The O_2 reduction and Proton Pumping Gate Mechanisms of Bovine Heart Cytochrome c Oxidase. (2011)
  • Author(s): Shinya Yoshikawa
  • Organizer: 欧州膜構造生物学協会(SBMPs)講演会
  • Place of Presentation: Paris(University of Paris), France
  • Year and Date: 2011-01-17
• [Presentation] チトクロム酸化酵素のO_2還元反応機構 (2010)
  • Author(s): 村本和優
  • Organizer: 日本生体エネルギー研究会第36回討論会
  • Place of Presentation: 大阪大学(大阪)
  • Year and Date: 2010-11-19
• [Presentation] ウシ心筋チトクロム酸化酵素の高分解能X線構造解析 (2010)
  • Author(s): 矢野直峰
  • Organizer: 日本生体エネルギー研究会第36回討論会
  • Place of Presentation: 大阪大学(大阪)
  • Year and Date: 2010-11-19
• [Presentation] X-ray structural analysis of catalytic F-intermediate in bovine cytochrome coxidase. (2010)
  • Author(s): 佐々木歩惟
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-22
• [Presentation] Proton pumping pathway of bovine heart cytochrome c oxidase. (2010)
  • Author(s): Ryota Aminaka
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-22
• [Presentation] The integrity of the bovine heart NADH-ubiquinone oxidoreductase responsible for the quality of the 2D crystals. (2010)
  • Author(s): Satoru Shimada
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-22
• [Presentation] The effects of detergents for quality of 2D crystal of bovine heart NADH-ubiquinone oxidoreductase. (2010)
  • Author(s): Satoko Amano
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-22
• [Presentation] Resonance Raman spectrum changes of FMN and iron-sulfur clusters of NADH-ubiquinone oxidoreductase upon redox state changes. (2010)
  • Author(s): Masahide Hikita
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-22
• [Presentation] Redox dependent structural change of cytochrome c oxidase at 1.4 Aresolution. (2010)
  • Author(s): 藤澤秀徳
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-21
• [Presentation] X-ray structural analysis of catalytic P-intermediate in bovine cytochrome coxidase. (2010)
  • Author(s): 木登梨佳
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-21
• [Presentation] X-ray structural analysis of catalytic O-intermediate in bovine cytochrome coxidase. (2010)
  • Author(s): 吉川信也
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-21
• [Presentation] Photo-dependent binding structures of CO and NO on the heme-copper site in bovine cytochrome c oxidase. (2010)
  • Author(s): 村本和優
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-21
• [Presentation] Non-Resonance Raman Evidence for Conformational Changes in the FoSubunits by Substrate Binding in Bovine FoF1-ATP Synthase. (2010)
  • Author(s): Kaoru Mieda
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-21
• [Presentation] Reaction mechanism of cytochrome c oxidase based on the O_2 analogues-bound structures. (2010)
  • Author(s): 村本和優
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-20
• [Presentation] High resolution X-ray structural analysis of cytochrome c oxidase to clarify the proton pumping mechanism at hydrogen atom level. (2010)
  • Author(s): 矢野直峰
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-20
• [Presentation] An X-ray structural evaluation of the possibility of chloride binding to the O_2 reduction site of bovine heart cytochrome c oxidase. (2010)
  • Author(s): 月原冨武
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-20
• [Presentation] Structural Relaxation of Cytochrome c Oxidase after CO Photolysis : Assignment of a Heme peripheral Raman Mode (2010)
  • Author(s): Izumi Ishigami
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-20
• [Presentation] Resonance Raman Evidence for the Heme-heme interaction in Cytochrome c Oxidase (2010)
  • Author(s): Miyuki Sakaguchi
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-20
• [Presentation] Femutosecond-Laser-Based Highly-Sensitive Time-Resolved IR Spectrometer for Studying Enzyme Reaction Under Physiological Conditions (2010)
  • Author(s): Minorca Kubo
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-20
• [Presentation] Photo-dependent binding structures of CO and NO on the heme-copper sitein bovine cytochrome c oxidase. (2010)
  • Author(s): 村本和優
  • Organizer: The 16th European Bioenergetics Conference
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2010-07-17
• [Presentation] The O_2 reduction and proton pumping gate mechanisms of bovine heart cytochrome c oxidase. (2010)
  • Author(s): 吉川信也
  • Organizer: The 16th European Bioenergetics Conference
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2010-07-17
• [Presentation] ウシ心筋チトクロム酸化酵素の酸素還元反応とプロトンポンプゲート機構 (2010)
  • Author(s): 吉川信也, 他
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2010-06-18
• [Presentation] ウシチトクロム酸化酵素のヘム・銅部位におけるNO、 COの光化学的結合解離構造 (2010)
  • Author(s): 村本和優
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2010-06-18
• [Presentation] チトクロム酸化酵素の水素位置決定を目的としたX線結晶構造解析 (2010)
  • Author(s): 菅倫寛
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2010-06-18
• [Presentation] 還元型ウシ心筋チトクロム酸化酵素の高分解能X線構造解析 (2010)
  • Author(s): 藤澤秀徳
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(札幌)
  • Year and Date: 2010-06-17
• [Presentation] The O_2 reduction and Proton Pumping Gate Mechanisms of Bovine Heart Cytochrome c Oxidase. (2010)
  • Author(s): Shinya Yoshikawa
  • Organizer: Workshop on Allosteric cooperativity in soluble, membrane bound hemoprotein and related membrane systems, organized by S.Papa, M.Brunori, T.Yonetani, S.Yoshikjawa
  • Place of Presentation: Bari (University of Bari), Italy
  • Year and Date: 2010-05-31
• [Presentation] The O_2 reduction and Proton Pumping Gate Mechanisms of Bovine Heart Cytochrome c Oxidase. (2010)
  • Author(s): Shinya Yoshikawa
  • Organizer: 欧州膜構造生物学協会(SBMPs)講演会
  • Place of Presentation: Toulouse (University of Toulouse), France
  • Year and Date: 2010-05-10
• [Presentation] チトクロム酸化酵素の水素原子観測に向けて
  • Author(s): 吉川信也
  • Organizer: 第１２回日本蛋白質科学会年会ワークショップ「中性子を用いた蛋白質化学研究の新展開」
  • Place of Presentation: 名古屋国際会議場（愛知県）
  • Invited
• [Presentation] An intermediate conformation of cytochrome oxidase in the ligand-free state identified by time-resolved resonance Raman spectroscopy
  • Author(s): Izumi Ishigami
  • Organizer: 日本生物物理学会第５０回年会
  • Place of Presentation: 名古屋大学東山キャンパス（愛知県）
• [Presentation] Time-resolved IR analysis of proteins using a novel pulse flow system synchronized with pump/probe lasers
  • Author(s): Minoru Kubo
  • Organizer: 日本生物物理学会第５０回年会
  • Place of Presentation: 名古屋大学東山キャンパス（愛知県）
• [Presentation] 電子線構造解析によるウシ心筋チトクロム酸化酵素・チトクロムｃ複合体の立体構造解明
  • Author(s): 押田幸歩
  • Organizer: 日本生体エネルギー研究会第３８回討論会
  • Place of Presentation: 岡山大学薬学部（岡山県）
• [Presentation] ウシ心筋ＮＡＤＨ―ユビキノン酸化還元酵素の２次元結晶化
  • Author(s): 島田悟
  • Organizer: 第１２回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] 電子線結晶構造解析によるウシ心筋チトクロム酸化酵素・チトクロムｃ複合体の立体構造解明
  • Author(s): 押田幸歩
  • Organizer: 第１２回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] 氷晶防止剤の導入方法によるウシ心筋チトクロム酸化酵素結晶の分解能の向上
  • Author(s): 畑中美紀
  • Organizer: 第１２回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] ウシ心筋チトクロム酸化酵素休止酸化型の高分解能Ｘ線結晶構造解析
  • Author(s): 矢野直峰
  • Organizer: 第１２回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] ウシ心筋チトクロム酸化酵素反応中間体のＸ線結晶構造解析
  • Author(s): 村本和優
  • Organizer: 第１２回日本蛋白質科学会年会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] X-ray structural analysis of bovine heart cytochrome c oxidase to clarify redox coupled structural changes at H-atom level
  • Author(s): Naomine Yano
  • Organizer: 日本生物物理学会第５０回年会
  • Place of Presentation: 名古屋大学東山キャンパス（愛知県）
• [Presentation] Characterization by mutagenesis analysis of putative proton transfer pathway, D-pathway of bovine heart cytochrome c oxidase
  • Author(s): Ryota Aminaka
  • Organizer: 日本生物物理学会第５０回年会
  • Place of Presentation: 名古屋大学東山キャンパス（愛知県）
• [Presentation] ミトコンドリアによる核の新規制御機構―チトクロムｃ酸化酵素のミトコンドリア由来サブユニットによる核呼吸因子(NRF)の発現機構
  • Author(s): 網中良太
  • Organizer: 日本生体エネルギー研究会第３８回討論会
  • Place of Presentation: 岡山大学薬学部（岡山県）
• [Presentation] ウシ心筋チトクロム酸化酵素の高分解能Ｘ線構造解析と水クラスターの役割
  • Author(s): 矢野直峰
  • Organizer: 日本生体エネルギー研究会第３８回討論会
  • Place of Presentation: 岡山大学薬学部（岡山県）
• [Presentation] Progress and challenges in the two-dimensional crystallization of intact bovine heart F-ATP synthase
  • Author(s): Christoph Gerle
  • Organizer: 日本生体エネルギー研究会第３８回討論会
  • Place of Presentation: 岡山大学薬学部（岡山県）
• [Remarks]
  • URL: http://www.sci.u-hyogo.ac.jp/life/regulation/publication.html