| Project/Area Number |
22248008
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ARAI Ryoichi 信州大学, 繊維学部, 助教 (50344023)
OGASAWARA Hiroshi 信州大学, ヒト環境科学研究支援センター, 助教 (30535232)
YAMAMOTO Hiroki 信州大学, 繊維学部, 准教授 (20262701)
ASAI Kei 埼玉大学, 大学院・理工学研究科, 准教授 (70283934)
HASHIMOTO Masayuki 首都大学東京, 理工学研究科, 研究員 (80402139)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥46,280,000 (Direct Cost: ¥35,600,000、Indirect Cost: ¥10,680,000)
Fiscal Year 2012: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2011: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2010: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
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| Keywords | 微生物機能 / 表層蛋白質 / 細胞形態 / 細胞壁溶解酵素 / インヒビター / 表層タンパク質 / 枯草菌 / ペプチドグリカン / シグマ因子 / 細胞表層 / 細胞増殖 / 発現制御 / 立体構造解析 / 細胞壁 |
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| Research Abstract |
Analysis of two cell wall hydrolase genes (cwlO and lytE) in Bacillus subtilisindicated that these two gene products were localized at the lateral side of cells and their autolytic DL-endopeptidase activity was required for cell growth. The activity is inhibited by IseA, and NMR analysis indicated IseA is a unique protein with a “hacksaw” structure. To maintain the cell surface, the sigma-directed regulation (SigI and anti-sigma RsgI) and the two-component system, WalK-WalR, were required at the stationary and vegetative growth phases, respectively. Peptidoglycan modification by wall teichoic acids was also investigated and the enzymatic properties of novel surface proteins were reported.
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