Mechanisms of cell growth, morphology, and maintenance by bacterial cell surface regulation
Project/Area Number |
22248008
|
Research Category |
Grant-in-Aid for Scientific Research (A)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied microbiology
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Research Institution | Shinshu University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
ARAI Ryoichi 信州大学, 繊維学部, 助教 (50344023)
OGASAWARA Hiroshi 信州大学, ヒト環境科学研究支援センター, 助教 (30535232)
YAMAMOTO Hiroki 信州大学, 繊維学部, 准教授 (20262701)
ASAI Kei 埼玉大学, 大学院・理工学研究科, 准教授 (70283934)
HASHIMOTO Masayuki 首都大学東京, 理工学研究科, 研究員 (80402139)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥46,280,000 (Direct Cost: ¥35,600,000、Indirect Cost: ¥10,680,000)
Fiscal Year 2012: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
Fiscal Year 2011: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2010: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000)
|
Keywords | 微生物機能 / 表層蛋白質 / 細胞形態 / 細胞壁溶解酵素 / インヒビター / 表層タンパク質 / 枯草菌 / ペプチドグリカン / シグマ因子 / 細胞表層 / 細胞増殖 / 発現制御 / 立体構造解析 / 細胞壁 |
Research Abstract |
Analysis of two cell wall hydrolase genes (cwlO and lytE) in Bacillus subtilisindicated that these two gene products were localized at the lateral side of cells and their autolytic DL-endopeptidase activity was required for cell growth. The activity is inhibited by IseA, and NMR analysis indicated IseA is a unique protein with a “hacksaw” structure. To maintain the cell surface, the sigma-directed regulation (SigI and anti-sigma RsgI) and the two-component system, WalK-WalR, were required at the stationary and vegetative growth phases, respectively. Peptidoglycan modification by wall teichoic acids was also investigated and the enzymatic properties of novel surface proteins were reported.
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Report
(4 results)
Research Products
(72 results)
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[Presentation] Cell surface modification and engineering in Bacillus subtilis2011
Author(s)
Sekiguchi, J., Kodama, T., Manabe, K., Kageyama, Y., Ara, K., Ozaki, K., Fukushima, T.
Organizer
IUMS2011
Place of Presentation
Sapporo(招待講演)
Year and Date
2011-09-10
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