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Establishment of a macaque cancer model using cancer cells derived from MHC homozygous macaque iPS cells

Research Project

Project/Area Number 22300143
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionShiga University of Medical Science

Principal Investigator

OGASAWARA Kazumasa  滋賀医科大学, 医学部, 教授 (20169163)

Co-Investigator(Kenkyū-buntansha) ITOH Yasushi  滋賀医科大学, 医学部, 准教授 (90324566)
ISHIGAKI Hirohito  滋賀医科大学, 医学部, 助教 (90432301)
TORII Ryuuzou  滋賀医科大学, 動物生命科学研究センター, 教授 (50106647)
Co-Investigator(Renkei-kenkyūsha) SHIINA Takashi  東海大学, 医学部, 准教授 (00317744)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
KeywordsiPS 細胞 / カニクイザル / 癌 / MHC / iPS細胞
Research Abstract

We made somatic cells from iPS cells derived from cynomolgus macaque, which homozygously had a certain haplotype of major histocompatibility complex (Mafa-HT1). And then, we transduced oncogenes to the differentiated cells resulting in establishment of malignant cells, an embryonal carcinoma PTY and a glioblastoma NPC#5PR2. These two cancer cell lines could make tumors in NOG mouse. We transplanted PTY cellsinto subcutaneous, ovary and abdominal cavity of a cynomolgus macaque with heterogygous MHC. However, all PTY cells were rejected after 4 weeks post transplantation. On the other hand, we are transplanting NPC#5PR2 cells into an immune privilege site, brain, of a Mafa HT-1 heterozygous monkey.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (14 results)

All 2013 2012 2010 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (9 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Epidemiology of Hepatitis E Virus in Indoor-Captive Cynomolgus Monkey Colony2012

    • Author(s)
      Nakamura S, Tsuchiya H, Okahara N, Nakagawa T, Ohara N, Yamamoto H, Li T-C, Takeda N, Ogasawara K, Torii R
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 74 Issue: 3 Pages: 279-283

    • DOI

      10.1292/jvms.11-0394

    • NAID

      130001190495

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Memory immune responses against pandemic (H1N1) 2009 influenza virus induced by a whole particle vaccine in ynomolgus monkeys carrying Mafa-A*052:022012

    • Author(s)
      Arikata M, Itoh Y, Okamatsu M, Maeda T, Shiina T, Tanaka K, Suzuki S, Nakayama M, Sakoda Y, Ishigaki H, Takada A, Ishida H, Soda K, Pham VL, Tsuchiya H, Nakamura S, Torii R, Shimizu T, Inoko H, Ohkubo I, Kida H, Ogasawara K
    • Journal Title

      PLos ONE

      Volume: 7 Issue: 5 Pages: e37220-e37220

    • DOI

      10.1371/journal.pone.0037220

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] カニクイザル iPS 細胞由来癌細胞株を用いた、担癌カニクイザルモデルの作製2013

    • Author(s)
      石垣宏仁、仲山美沙子、伊藤靖、小笠原一誠
    • Organizer
      第102回病理学会総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] iPS研究の前臨床試験のためのカニクイザルMHC遺伝子の多型解析2013

    • Author(s)
      椎名 隆
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] フィリピン産カニクイザルのMHC多型解析によるホモ接合個体の特定2012

    • Author(s)
      椎名 隆
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] Hemoperfusion of tumor-bearing rats with an adsorbent column specific for LAP positive T cells suppressed the tumor growth2012

    • Author(s)
      Teramoto K
    • Organizer
      he 3rd Workshop of Synthetic Immunology
    • Place of Presentation
      Kyoto
    • Related Report
      2012 Annual Research Report
  • [Presentation] カニクイザル MHC ホモ接合個体の特定2010

    • Author(s)
      椎名隆、小笠原一誠、鳥居隆三等
    • Organizer
      第19回日本組織適合性学会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] カニクイザルMHCホモ接合個体の特定2010

    • Author(s)
      椎名隆、小笠原一誠、鳥居隆三, 等
    • Organizer
      第19回日本組織適合性学会
    • Place of Presentation
      東京
    • Related Report
      2010 Annual Research Report
  • [Presentation] カニクイザルiPS細胞由来癌細胞株を用いた、担癌カニクイザルモデルの作製

    • Author(s)
      石垣宏仁
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] An inactivated whole particle vaccine induced memory immune responses against pandemic (H1N1) 2009 influenza virus in cynomolgus monkeys carrying Mafa-A1*052:02

    • Author(s)
      Itoh Y
    • Organizer
      第16回日本ワクチン学会学術集会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] herapeutic efficacy of a human-mouse chimeric anti-H5 hemagglutinin antibody on lethal H5N1 highly pathogenic avian influenza virus infection in cynomolgus macaques

    • Author(s)
      Itoh Y
    • Organizer
      第41回日本免疫学会学術集会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.shiga-med.ac.jp/~hqpatho2/

    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] カニクイザル MHC 全遺伝子ホモ接合体を作成するためのキット及び方法2010

    • Inventor(s)
      小笠原一誠、鳥居隆三、椎名隆
    • Industrial Property Rights Holder
      滋賀医科大学
    • Industrial Property Number
      2010-105956
    • Filing Date
      2010-04-30
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] MHChomozygousカニクイザルの作成2010

    • Inventor(s)
      小笠原一誠、鳥居隆三、椎名隆
    • Industrial Property Rights Holder
      滋賀医科大学
    • Filing Date
      2010-04-30
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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