Bioengineering of artificial red cells for clinical application

• Project/Area Number: 22300161
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Waseda University
• Principal Investigator: SAKAI Hiromi 早稲田大学, 重点領域研究機構, 教授 (70318830)
• Co-Investigator(Renkei-kenkyūsha): KOBAYASHI Koichi 慶應義塾大学, 医学部, 名誉教授 (80051704)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2011: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2010: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: 人工臓器工学 / 再生医工学 / 人工血液 / リポソーム / 輸血代替 / 酸素輸液 / 血液代替物 / 臓器灌流 / 再生医療 / 分子集合体 / ヘモレオロジー / 人工赤血球 / ヘモグロビン

Research Abstract

One of the characteristics of artificial red cells (hemoglobin-vesicles) as a molecular assembly is that their physicochemical properties can be manipulated. New function can also be added to the particles. In this reasech we take the advantages of such artificial red cells to apply for new clinical indications which blood transfusion cannot be attained. We establish “gas bioengineering” using artificial red cells.

Research Products

• [Journal Article] Swine hemoglobin as a potential source of artificial oxygen carriers, hemoglobin-vesicles. (2013)
  • Author(s): Hiromi Sakai
  • Journal Title: Artif. Cells Nanomedicine Biotechnol. Volume: 41 Issue: 1 Pages: 37-41
  • DOI: 10.3109/10731199.2012.716067
  • Peer Reviewed
• [Journal Article] Fourteen-days observation and pharmacokinetic evaluation after massive intravenous infusion of hemoglobin-vesicles (artificial oxygen carriers) in cynomolgus monkeys. (2012)
  • Author(s): K. Taguchi, H. Watanabe, H. Sakai, H. Horinouchi, K. Kobayashi, T. Maruyama, M. Otagiri
  • Journal Title: J. Drug Metab. Toxicol Volume: 3 Pages: 1000128-1000128
• [Journal Article] Physiolgical capacity of the reticuloendothelial system for the degradation of hemoglobin-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusion for 7 days in Pregnant rats and fetuses. (2012)
  • Author(s): M. Kaga, H. Ohta, Y. Lee, R. Kamii, H. Yamamoto, S. Akiyama, S. Watanabe, T. Matsuda, Y. Kimura, S. Tsuchiya, H. Tei, L. Okamura, H. Sakai, N. Yaegashi
  • Journal Title: Life Sci. Volume: 91 Pages: 420-428
• [Journal Article] Cardiopulmonary hemodynamic responses to the small injection of hemoglobin-vesicles (artificial oxygen carriers) in miniature pigs (2012)
  • Author(s): H. Sakai, Y. Suzuki, K. Sou, M. Kano
  • Journal Title: J. Biomed. Mater. Res. A Volume: 100 Pages: 2668-2677
• [Journal Article] Microcirculation and NO-CO studies of a natural extracellular hemoglobin developed for an oxygen therapeutic carrier (2012)
  • Author(s): A.G. Tsai, M. Intaglietta, H. Sakai, E. Delpy, C.D. la Rochelle, M. Rousselot, F. Zal
  • Journal Title: Current Drug Discovery Techonl. Volume: 9 Pages: 166-172
• [Journal Article] Effect of the cellular-type artificial oxygen carrier Hb-vesicle as a resuscitative fluid for pre-hospital treatment : Experiments in a rat uncontrolled hemorrhagic shock model. (2012)
  • Author(s): Y. Seishi, H. Horinouchi, H. Sakai, K. Kobayashi
  • Journal Title: Shock Volume: 38 Pages: 153-158
• [Journal Article] Removal of Hemoglobin-vesicles from circulating blood by using centrifugation and ultrafiltration. (2012)
  • Author(s): H. Sakai, K. Sou, H. Horinouchi, E. Tsuchida, K. Kobayashi.
  • Journal Title: Artif. Organs Volume: 36 Pages: 202-209
• [Journal Article] Fluid resuscitation of hemorrhagic shock with Hemoglobin vesicles in Beagle dogs : pilot study. (2012)
  • Author(s): M. Yamamoto, H. Horinouchi, Y. Seishi, N. Sato, M. Itoh, K. Kobayashi, H. Sakai
  • Journal Title: Artif. Cells Blood Substitutes Biotechnol Volume: 40 Pages: 179-195
• [Journal Article] Protein-protein interactions in solution and their interplay with protein specific functions (2012)
  • Author(s): T. Sato, T. Fukasawa, T. Komatsu, H. Sakai, S. Ishiwata
  • Journal Title: J. Phys. Soc. Jpn Volume: 81
• [Journal Article] Cellular-type hemoglobin-based oxygen carrier (hemoglobin-vesicles) as a transfusion alternative and for oxygen therapeutics (2012)
  • Author(s): H. Sakai
  • Journal Title: Curr. Drug Discovery Techonl Volume: 9 Pages: 188-193
• [Journal Article] Protein-protein interactions in solution and their interplay with protein specific functions (2012)
  • Author(s): Sato, T., et al.
  • Journal Title: Phys. Soc. Jpn. Volume: 81 Issue: Suppl.A Pages: SA002-SA002
  • DOI: 10.1143/jpsjs.81sa.sa002
  • Peer Reviewed
• [Journal Article] Cellular-type hemoglobin-based oxygen carrier (hemoglobin-vesicles) as a transfusion alternative and for oxygen therapeutics (2012)
  • Author(s): Hiromi Sakai
  • Journal Title: Current Drug Discovery Techonl. Volume: 9 Issue: 3 Pages: 188-193
  • DOI: 10.2174/157016312802650805
  • Peer Reviewed
• [Journal Article] Hemoglobin vesicle as an effective blood substitute and its removal from circulating blood (2012)
  • Author(s): H.Sakai, K.Sou, H.Horinouchi, E.Tsuchida, K.Kobayashi
  • Journal Title: Artificial Organs Volume: 36 Issue: 2 Pages: 202-209
  • DOI: 10.1111/j.1525-1594.2011.01236.x
  • Peer Reviewed
• [Journal Article] Fluid resuscitation of hemorrhagic shock with Hemoglobin vesicles in Beagle dogs : pilot study (2012)
  • Author(s): M.Yamamoto, H.Horinouchi, Y.Seishi, K.Kobayashi, H.Sakai, et al
  • Journal Title: Artif.Cells Blood Substitutes Biotechnol Volume: 40 Issue: 1-2 Pages: 179-195
  • DOI: 10.3109/10731199.2011.637929
  • Peer Reviewed
• [Journal Article] Intravenous injection of Hb-vesicles (artificial oxygen carriers) after hemodilution with a series of plasma expanders (water-soluble biopolymers) in a rat repeated hemorrhage model. (2011)
  • Author(s): H. Sakai, N. Miyagawa, H. Horinouchi, S. Takeoka, M. Takaori, E. Tsuchida, K. Kobayashi
  • Journal Title: Polymers Adv. Technol. Volume: 22 Pages: 1216-1222
• [Journal Article] Fluid resuscitation with hemoglobin vesicles prevents Esherichia coli growth via complement activation in a hemorrhagic shock rat model. (2011)
  • Author(s): K. Taguchi, S. Ogaki, H. Watanabe, D. Kadowaki, H. Sakai, K. Kobayashi, H. Horinouchi, T. Maruyama, M. Otagiri
  • Journal Title: J. Pharmacol. Exp. Therap Volume: 337 Pages: 201-208
• [Journal Article] Phagocytosis of liposome particles by rat splenic immature monocytes makes them transiently and highly immunosuppressive in ex vivo culture condition. (2011)
  • Author(s): D. Takahashi, H. Azuma, H. Sakai, K. Sou, D. Wakita, H. Abe, M. Fujihara, H. Horinouchi, K. Kobayashi, T. Nishimura, H. Ikeda.
  • Journal Title: J. Pharmacol. Exp. Therap Volume: 337 Pages: 42-49
• [Journal Article] Repeated injection of high dose of hemoglobin encapsulated liposomes (hemoglobin-vesicles) induces accelerated blood clearance in a hemorrhagic shock rat model. (2011)
  • Author(s): K. Taguchi, Y. Iwao, H. Watanabe, D. Kadowaki, H. Sakai, K. Kobayashi, H. Horinouchi, T. Maruyama, M. Otagiri
  • Journal Title: Drug Metab. Dispos Volume: 39 Pages: 484-489
• [Journal Article] Increased viscosity of hemoglobin-based oxygen carriers retards NO-binding when perfused through narrow gas-permeable tubes (2011)
  • Author(s): H. Sakai, N. Okuda, S. Takeoka, E. Tsuchida
  • Journal Title: Microvasc. Res. Volume: 81 Pages: 169-176
• [Journal Article] Alteration in the pharmacokinetics of hemoglobin-vesicles in a rat model of chronic liver cirrhosis is associated with Kupffer cell phagocyte activity. (2011)
  • Author(s): K. Taguchi, M. Miyasato, H. Watanabe, H. Sakai, H. Horinouchi, K. Kobayashi, E. Tsuchida, T. Maruyama, M. Otagiri
  • Journal Title: J. Pharmaceut. Sci. Volume: 100 Pages: 775-783
• [Journal Article] An automated method for cell-free layer width determination in small arterioles. (2011)
  • Author(s): P.K. Ong, S. Jain, B. Namgung, Y.I. Woo, H. Sakai, D. Lim, K.J. Chun, S. Kim
  • Journal Title: Physiol. Meas Volume: 32
• [Journal Article] Gas bioengineering using hemoglobin-vesicles for versatile clinical application (2011)
  • Author(s): H. Sakai, S. Takeoka, K. Kobayashi
  • Journal Title: Curr. Pharmaceut. Design Volume: 17 Pages: 2352-2359
• [Journal Article] What is the major mechanism of slower NO uptake by red blood cells? (Letter to Editorial) (2011)
  • Author(s): H. Sakai
  • Journal Title: J. Biol. Chem. Volume: 286
• [Journal Article] Intravenous injection of Hb-vesicles after hemodilution with a series of plasma expanders (water-soluble biopolymers) in a rat repeated hemorrhage model (2011)
  • Author(s): H.Sakai, N.Miyagawa, H.Horinouchi, S.Takeoka, M.Takaori, et al
  • Journal Title: Polymers Adv.Technol. Volume: 22 Issue: 8 Pages: 1216-1222
  • DOI: 10.1002/pat.1964
  • Peer Reviewed
• [Journal Article] Cellular-type hemoglobin-based oxygen carrier (hemoglobin-vesicles) as a transfusion alternative and for oxygen therapeutics. (2011)
  • Author(s): H.Sakai
  • Journal Title: Current Drug Discovery Techonl Volume: (発行予定) Pages: 364-364
  • Peer Reviewed
• [Journal Article] Effects of crystalloid and colloid osmotic pressure on HbV (liposome encapsulated hemoglobin) membrane. (2010)
  • Author(s): Miyake, H. Sakai, M. Takaori
  • Journal Title: Artif. Blood Volume: 18 Pages: 128-133
• [Journal Article] Measurement of electrolyte concentrations in serum containing liposome vesicles (2010)
  • Author(s): Miyake, J. Takemura, H. Sakai, M. Takaori
  • Journal Title: Artif. Blood Volume: 18 Pages: 91-95
• [Journal Article] Hepatically-metabolized and .excreted artificial oxygen carrier, hemoglobin-vesicles, can be safely used under conditions of hepatic impairment. (2010)
  • Author(s): K. Taguchi, M. Miyasato, H. Ujihara, H. Watanabe, D. Kadowaki, H. Sakai, E. Tsuchida, H. Horinouchi, K. Kobayashi, T. Maruyama, M. Otagiri
  • Journal Title: Toxicol. Appl. Pharmacol. Volume: 248 Pages: 234-241
• [Journal Article] Hemoglobin-vesicle, a cellular artificial oxygen carrier, that fulfils the physiological roles of the red blood cells structure. (2010)
  • Author(s): H. Sakai, K. Sou, H. Horinouchi, K. Kobayashi, E. Tsuchida
  • Journal Title: Adv. Exp. Med. Biol. Volume: 662 Pages: 433-438
• [Journal Article] Increased viscosity of hemoglobin-based oxygen carriers retards NO-binding when perfused through narrow gas-permeable tubes (2010)
  • Author(s): H.Sakai, N.Okuda, S.Takeoka, E.Tsuchida
  • Journal Title: Microvasc.Res. Volume: 81 Pages: 169-176
  • Peer Reviewed
• [Presentation] Gas Bioengineering of Artificial Red Cells (invited) (2012)
  • Author(s): H. Sakai
  • Organizer: 28th Annual Conference, on 40th Anniversary of Faculty of Medicine, Prince of Songkla University
  • Place of Presentation: Hat Yai, Thailand
  • Year and Date: 2012-08-08
• [Presentation] 人工赤血球(ヘモグロビン小胞体)の物理化学的特徴と体内酸素輸送機能 (2012)
  • Author(s): 酒井宏水
  • Organizer: 第13回医療化学懇談会
  • Place of Presentation: 国立国際医療研究センター(東京都)(招待講演)
  • Year and Date: 2012-01-21
• [Presentation] 人工赤血球(ヘモグロビン小胞体)をブタに少量投与したときの血行動態に関する検討 (2012)
  • Author(s): 酒井宏水、鈴木勇司、宗慶太郎、狩野真由美
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール
• [Presentation] 人工赤血球/代用血漿剤(水溶性高分子)分散系のレオロジー挙動 (2012)
  • Author(s): 酒井宏水
  • Organizer: 第60回レオロジー討論会
  • Place of Presentation: 名古屋大学
• [Presentation] リポソーム製剤としての人工赤血球の効率の高い製造法 (2012)
  • Author(s): 酒井宏水
  • Organizer: 産学官連携推進会議<第11回>イノベーションジャパン2012
  • Place of Presentation: 東京フォーラム
• [Presentation] Artificial Red Cells (Hemoglobin-vesicles) as a Cellular-type Hemoglobin-based Oxygen Carrier for Versatile Clinical Applications (2012)
  • Author(s): H. Sakai
  • Organizer: BIT's 1st Annual International Symposium of Hematology
  • Place of Presentation: Beijing
• [Presentation] Cardiopulmonary hemodynamic responses to the injection of hemoglobin-vesicles (artificial oxygen carriers) in miniature pigs (2012)
  • Author(s): H. Sakai, Y. Suzuki, K. Sou, M. Kano
  • Organizer: 9th World Biomaterials Congress
  • Place of Presentation: Chengdu
• [Presentation] Cardiopulmonary hemodynamic responses to the injection of hemoglobin-vesicles (artificial oxygen carriers) in miniature pigs (2012)
  • Author(s): Hiromi Sakai
  • Organizer: 9th World Biomaterials Congress
  • Place of Presentation: Chengdu, China
• [Presentation] Gas Bioengineering of Artificial Red Cells (2012)
  • Author(s): Hiromi Sakai
  • Organizer: 28th Annual Conference, on 40th Anniversary of Faculty of Medicine, Prince of Songkla University
  • Place of Presentation: Hat Yai, Thailand
  • Invited
• [Presentation] Artificial Red Cells (Hemoglobin-vesicles) as a Cellular-type Hemoglobin-based Oxygen Carrier for Versatile Clinical Applications (2012)
  • Author(s): Hiromi Sakai
  • Organizer: BIT’s 1st Annual International Symposium of Hematology
  • Place of Presentation: Beijing, China
• [Presentation] 日本の人工酸素運搬体の歴史と現状 (2011)
  • Author(s): 酒井宏水
  • Organizer: 第18回日本血液代替物学会
  • Place of Presentation: 北海道大学医学部学友会館フラテ(北海道)(招待講演)
  • Year and Date: 2011-10-28
• [Presentation] Hemoglobin nanoencapsulation favorably retards gas reactions (2011)
  • Author(s): H. Sakai
  • Organizer: 2nd anniversary symposium of Waseda Bioscience Research Institute in Singapore
  • Place of Presentation: Matrix, Biopolis, Singapore
  • Year and Date: 2011-09-29
• [Presentation] Hemoglobin nanoencapsulation favorably retards gas reactions (2011)
  • Author(s): Hiromi Sakai
  • Organizer: 2nd anniversary symposium of Waseda Bioscience Research Institute in Singapore
  • Place of Presentation: シンガポール(招待講演)
  • Year and Date: 2011-09-29
• [Presentation] シンガポールにおける人工赤血球の研究(WABIOS ランチョンセミナー) (2011)
  • Author(s): H. Sakai
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大書写キャンパス
  • Year and Date: 2011-09-18
• [Presentation] Hb encapsulation in vesicles retards gas reactions by intracellular diffusion barrier and decreased diffusion of vesicles (2011)
  • Author(s): H. Sakai
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大書写キャンパス
  • Year and Date: 2011-09-16
• [Presentation] Hb encapsulation in vesicles retards gas reactions by intracellular diffusion barrier and decreased diffusion of vesicles (2011)
  • Author(s): Hiromi Sakai
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学書写キャンパス(兵庫県)
  • Year and Date: 2011-09-16
• [Presentation] Characteristics of hemoglobin vesicles as a cellular type artificial oxygen carrier (2011)
  • Author(s): Hiromi Sakai
  • Organizer: XIII International Symposium on Blood Substitutes and Oxygen Therapeutics
  • Place of Presentation: ボストン(米国)(招待講演)
  • Year and Date: 2011-07-28
• [Presentation] Hb encapsulation in vesicles retards NO- and CO-binding and O2-release (2011)
  • Author(s): Hiromi Sakai
  • Organizer: International Society on Oxygen Transport to Tissue 2011
  • Place of Presentation: ワシントンDC(米国)
  • Year and Date: 2011-07-24
• [Presentation] Artificial Red Cells (Hemoglobin-Vesicles) for a Transfusion Alternative and Oxygen Therapeutics (2011)
  • Author(s): Hiromi Sakai
  • Organizer: International Conference on Materials for Advanced Technologies
  • Place of Presentation: シンガポール
  • Year and Date: 2011-06-27
• [Presentation] Artificial oxygen carriers (hemoglobin-vesicles) as a transfusion alternative and for oxygen therapeutics (2011)
  • Author(s): Hiromi Sakai
  • Organizer: The 5th Kuala Lumpur International Conference on Biomodical Engineering
  • Place of Presentation: クアラルンプール(マレーシア)
  • Year and Date: 2011-06-23
• [Presentation] Developing next generation artificial oxygen carrier (artificial red cells) for versatile clinical application (2011)
  • Author(s): H.Sakai
  • Organizer: Drug Discovery World Asia 2011
  • Place of Presentation: Marina Bay Sands, Singapore(招待講演)
  • Year and Date: 2011-03-30
• [Presentation] 人工赤血球 : ヘモグロビン小胞体の新しい臨床応用 (2011)
  • Author(s): 酒井宏水
  • Organizer: Technology Link in WTLO 早稲田大学産官学研究推進センター
  • Place of Presentation: 早稲田大学55 号館N 棟大会議室
  • Year and Date: 2011-01-14
• [Presentation] 人工赤血球:ヘモグロビン小胞体の新しい臨床応用 (2011)
  • Author(s): 酒井宏水
  • Organizer: Technology Link in WTLO
  • Place of Presentation: 早稲田大学(招待講演)
  • Year and Date: 2011-01-14
• [Presentation] 日本の人工酸素運搬体の歴史と現状 (2011)
  • Author(s): 酒井宏水
  • Organizer: 第18回日本血液代替物学会
  • Place of Presentation: 北海道大学医学部学友会館フラテ
• [Presentation] Characteristics of hemoglobin vesicles as a cellular type artificial oxygen carrier (2011)
  • Author(s): H. Sakai, H. Horinouchi, K. Kobayashi
  • Organizer: XIII International Symposium on Blood Substitutes and Oxygen Therapeutics
  • Place of Presentation: Boston, USA
• [Presentation] Hb encapsulation in vesicles retards NO- and CO-binding and O_2-release (2011)
  • Author(s): H. Sakai
  • Organizer: International Society on Oxygen Transport to Tissue 2011
  • Place of Presentation: Georgetown Univ., Washington DC, USA
• [Presentation] Artificial Red Cells (Hb-Vesicles) for a Transfusion Alternative and Oxygen Therapeutics (2011)
  • Author(s): H. Sakai
  • Organizer: International Conference on Materials for Advanced Technologies.
  • Place of Presentation: Suntec, Singapore
• [Presentation] Artificial oxygen carriers (Hbvesicles) as a transfusion alternative and for oxygen therapeutics (2011)
  • Author(s): H. Sakai
  • Organizer: The 5th Kuala Lumpur International Conference on Biomedical Engineering
  • Place of Presentation: Kuala Lumpur, Malaysia
• [Presentation] Developing next generation artificial oxygen carrier (artificial red cells) for versatile clinical application (2011)
  • Author(s): H. Sakai
  • Organizer: Drug Discovery World Asia 2011
  • Place of Presentation: Singapore
• [Presentation] Characteristics of Artificial Red Cells (hemoglobin-vesicles) as a transfusion alternative and for oxygen therapeutics (Invited) (2010)
  • Author(s): H. Sakai
  • Organizer: Department of Biochemistry Seminars
  • Place of Presentation: Biochemistry, School of Medicine, National Univ. of Singapore
  • Year and Date: 2010-11-29
• [Presentation] 人工赤血球(ヘモグロビン小胞体)の開発状況(Invited) (2010)
  • Author(s): 酒井宏水
  • Organizer: 信州大学繊維学部G-COE 国際特別講演会
  • Year and Date: 2010-11-22
• [Presentation] Physicochemical importance of the red blood cells structure in the designing of artificial red cells (Invited) (2010)
  • Author(s): H. Sakai
  • Organizer: Seminar at Department of Bioengineering
  • Place of Presentation: University of California, San Diego
  • Year and Date: 2010-11-19
• [Presentation] Hemoglobin-vesicles as artificial oxygen carriers based on bioconjugate chemistry and nanobiotechnology for versatile clinical application (2010)
  • Author(s): H.Sakai
  • Organizer: BIT's 1st Annual World Conference of NanoMedicine-2010
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-10-23
• [Presentation] Hemoglobin-vesicles as oxygen carriers for versatile clinical application (2010)
  • Author(s): H. Sakai
  • Organizer: Applied Chemistry Lecture Series of Changchun Institute of Applied Chemistry, Chinese Academy of Sciences (CIAC)
  • Place of Presentation: Changchun, China
  • Year and Date: 2010-10-21
• [Presentation] ヘモグロビン小胞体の開発の歴史と現状 (2010)
  • Author(s): 酒井宏水
  • Organizer: 第17回日本血液代替物学会年次大会
  • Place of Presentation: 熊本市国際交流会館(招待講演)
  • Year and Date: 2010-10-17
• [Presentation] Nano dimension hemoglobin lipid vesicles as oxygen carriers for versatile clinical applications (2010)
  • Author(s): H.Sakai
  • Organizer: The 2nd World Congress of the International Academy of Nanomedicine
  • Place of Presentation: Antalya, Turkey(招待講演)
  • Year and Date: 2010-10-03
• [Presentation] HB-VESICLES AS AN ARTIFICIAL O2 CARRIER : THE REACTION PROFILES WITH GAS MOLECULES (O2, NO, AND CO) (2010)
  • Author(s): H.Sakai
  • Organizer: The 5th SBE International Conference on Bioengineering and Nanotechnology
  • Place of Presentation: Biopolis, Singapore
  • Year and Date: 2010-08-06
• [Presentation] Characteristics of Hb-vesicles as a cellular type Hb-based oxygen carrier (2010)
  • Author(s): H.Sakai
  • Organizer: The 2nd International Conference on Cellular & Molecular Bioen gineering
  • Place of Presentation: Nanyang Technol.Univ.
  • Year and Date: 2010-08-02
• [Presentation] 人工赤血球:ヘモグロビン小胞体の新しい臨床応用 (2010)
  • Author(s): 酒井宏水
  • Organizer: 第9回国際バイオEXPO
  • Place of Presentation: 東京ビックサイト(招待講演)
  • Year and Date: 2010-06-30
• [Presentation] Hemoglobin-vesicles as artificial oxygen carriers based on bioconjugate chemistry and nanobiotechnology for versatile clinical application (2010)
  • Author(s): H. Sakai
  • Organizer: BIT's 1st Annual World Conference of NanoMedicine-2010
  • Place of Presentation: International Convention Center / Beijing, China
• [Presentation] ヘモグロビン小胞体の開発の歴史と現状 (2010)
  • Author(s): 酒井宏水
  • Organizer: 第17回日本血液代替物学会年次大会
  • Place of Presentation: 熊本市国際交流会館
• [Presentation] Nano dimension hemoglobin lipid vesicles as oxygen carriers for versatile clinical applications (2010)
  • Author(s): H. Sakai, H. Horinouchi, E. Tsuchida, K. Kobayahi
  • Organizer: The 2nd World Congress of the International Academy of Nanomedicine
  • Place of Presentation: Calista Luxury Resort, Antalya, Turkey
• [Presentation] HEMOGLOBIN-VESICLES, ARTIFICIAL RED CELLS, AS O_2 AND CO CARRIERS FOR CYTOPROTECTION (2010)
  • Author(s): H. Sakai, H. Horinoichi, K. Kobayashi, E. Tsuchida
  • Organizer: The 5th SBE International Conference on Bioengineering and Nanotechnology
  • Place of Presentation: Biopolis, Singapore
• [Presentation] 人工赤血球 : ヘモグロビン小胞体の新しい臨床応用 (2010)
  • Author(s): 酒井宏水
  • Organizer: 第9回国際バイオEXPO
  • Place of Presentation: 東京ビックサイト
• [Book] Hemoglobin-based oxygen carriers; principles, approaches and current status (H.W. Kim & A.G. Greenburg eds.) (2013)
  • Author(s): Hiromi Sakai
  • Publisher: Springer-Verlag
• [Book] Academia-industry collaboration in blood substitute development. - issues, case histories and a proposal. In : Chemistry and Biochemistry of Oxygen Therapeutics : from Transfusion to Artificial Blood. (Ed. by S. Bettati and A. Mozzarelli), Chapter 29 (2011)
  • Author(s): H.W. Kim, A. Mozzarelli, H. Sakai, J. Jahr
  • Publisher: John Wiley & Sons
• [Book] Hemoglobin-vesicles as a cellular type hemoglobin-based oxygen carrier. In : Chemistry and Biochemistry of Oxygen Therapeutics : from Transfusion to Artificial Blood. (Ed. by S. Bettati and A. Mozzarelli), Chapter 27 (2011)
  • Author(s): H. Sakai, H. Horinouchi, E. Tsuchida, K. Kobayashi
  • Publisher: John Wiley & Sons
• [Book] Artificial oxygen carriers (hemoglobin-vesicles) as a transfusion alternative and for oxygen therapeutics (2011)
  • Author(s): H. Sakai
  • Publisher: IFMBE Proceedings 35 (5th Kuala Lumpur International Conference on Biomedical Engineering 2011 (BIOMED 2011)
• [Book] Hb-vesicles as a cellular type Hb-based O2 carrier. In : Chemistry and Biochemistry of Oxygen The rapeutics(Bettati & Mozzarelli, Ed.) (2011)
  • Author(s): H.Sakai, H.Horinouchi, E.Tsuchida, K.Kobayashi
  • Total Pages: 442
  • Publisher: John Wiley & Sons
• [Book] Academia-industry collaboration in blood substitute development. In : Chemistry and Biochemistry of Oxygen Therapeutics(Bettati & Mozzarolli, Ed.) (2011)
  • Author(s): H.W.Kim, A.Mozzarelli, H.Sakai, J.Jahr
  • Total Pages: 442
  • Publisher: John Wiley & Sons
• [Book] Cellular-type hemoglobin-based oxygen carriers to mimic the red blood cells structure. In : Hemoglobin-based oxygen carriers .principles, approaches and current status (H.W. Kim & A.G. Greenburg eds.)
  • Author(s): H. Sakai
  • Publisher: Springer-Verlag (Berlin/Heidelberg, Germany) (in press)
• [Book] Biocompatibility of a highly concentrated fluid of Hemoglobin-vesicles as a transfusion alternative. In : Selective Topics in Nanomedicine (T.M.S. Chang ed.)
  • Author(s): H. Sakai
  • Publisher: World Scientific, Singapore (in press)
• [Remarks]
  • URL: http://www.naramed-u.ac.jp/~chem/
• [Remarks] NHK E テレ「サイエンスZERO」人工赤血球の研究を紹介(2012年7月15日) 報道等
• [Remarks] J-CAST ニュースに紹介記事。「早大グループが「人工赤血球」を開発大学見本市で発表」(2012年10月19日) 報道等
• [Remarks] 日本経済新聞朝刊に紹介記事。「知の明日を築く、アジア発、生命科学研究」(2012年12月6日) 報道等
• [Patent(Industrial Property Rights)] METHOD FOR PRODUCTION OF VESICLES ENCAPSULATING FUNCTIONAL MATERIALS (2013)
  • Inventor(s): 酒井宏水
  • Industrial Property Rights Holder: 早稲田大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-04-04
  • Overseas
• [Patent(Industrial Property Rights)] 小胞体の製造法 (2012)
  • Inventor(s): 酒井宏水
  • Industrial Property Rights Holder: 早稲田大学
  • Filing Date: 2012-04-04
  • Overseas
• [Patent(Industrial Property Rights)] 小胞体の製造法 (2012)
  • Inventor(s): 酒井宏水
  • Industrial Property Rights Holder: 早稲田大学
  • Filing Date: 2012-04-04