Development of Laser-induced Tumor Model in Targeted Tissues

• Project/Area Number: 22300322
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Carcinogenesis
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: YUBA Shunsuke 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究グループ長 (40263248)
• Co-Investigator(Kenkyū-buntansha): DEGUCHI Tomonori 独立行政法人産業技術総合研究所, 健康工学研究部門, 研究員 (30415715) ; KAWASAKI Takashi 独立行政法人産業技術総合研究所, 健康工学研究部門, 主任研究員 (60356839)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2012: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2010: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
• Keywords: 癌 / 病理学 / 発癌 / メダカ

Research Abstract

We aimed at development of Tumor Model in Targeted Tissues using Infrared laser-evoked gene operator(IR-LEGO). First, we have established transgenic(TG) medaka lines which pancreatic cells were visualized as the target of laser irradiation, and another li

Research Products

• [Journal Article] Intergenic region between TATA-box binding protein and proteasome subunit C3 genes of Medaka function as the bidirectional promoter in vitro and in vivo (2012)
  • Author(s): Fujimori KE, Hazama K, Kawasaki T, Deguchi T, Yuba S
  • Journal Title: Gene. Volume: 511(2) Pages: 177-86
• [Journal Article] Transgenic medaka fish which mimic the endogenous expression of neuronal kinesin, KIF5A (2012)
  • Author(s): Kawasaki T, Kurauchi K, Higashihata A, Deguchi T, Ishikawa Y, Yamauchi M, Sasanuma M, Hori H, Tsutsumi M, Wakamatsu Y, Yuba S, Kinoshita M
  • Journal Title: Brain Res Volume: 1480 Pages: 12-21
  • NAID: 120004874013
• [Journal Article] In vivo visualization of the lymphatic vessels in pFLT4-EGFP transgenic medaka (2012)
  • Author(s): Deguchi, T., Fujimori, KE., Kawasaki, T.,Maruyama, K., Yuba, S
  • Journal Title: Genesis Volume: 50(8) Pages: 625-34
  • Peer Reviewed
• [Journal Article] In vivo visualization of the lymphatic vessels in pFLT4-EGFP transgenic medaka. (2012)
  • Author(s): Deguchi, T., Fujimori, KE., Kawasaki, T., Maruyama, K., Yuba, S.
  • Journal Title: Genesis Volume: 50 Pages: 625-34
  • Peer Reviewed
• [Journal Article] Transgenic medaka fish which mimic the endogenous expression of neuronal kinesin, KIF5A. (2012)
  • Author(s): Kawasaki T, Kurauchi K, Higashihata A, Deguchi T, Ishikawa Y, Yamauchi M, Sasanuma M, Hori H, Tsutsumi M, Wakamatsu Y, Yuba S, Kinoshita M.
  • Journal Title: Brain Res. Volume: 1480 Pages: 12-21
  • NAID: 120004874013
  • Peer Reviewed
• [Journal Article] Intergenic region between TATA-box binding protein and proteasome subunit C3 genes of Medaka function as the bidirectional promoter in vitro and in vivo. (2012)
  • Author(s): Fujimori KE, Hazama K, Kawasaki T, Deguchi T, Yuba S.
  • Journal Title: Gene Volume: 511 Pages: 177-86
  • Peer Reviewed
• [Journal Article] Identification of a functional Medaka heat shock promoter andcharacterization of its ability to induce in vitro and in vivoexogenous gene expression in Medaka (2010)
  • Author(s): Oda, S., Mikami, S., Urushihara,Y., Murata, Y., Kamei, Y., Deguchi, T., Kitano, T., Fujimori, K.,Yuba, S., Todo, T., Mitani, H
  • Journal Title: Zoological Science Volume: 27(5) Pages: 410-5
• [Journal Article] 赤外レーザーによる局所的遺伝子発現誘導法 (2010)
  • Author(s): 浦和博子、出口友則、木村英二、伊藤真理子、鈴木基史、岡田清孝、八田公平、高木新、 弓場俊輔、亀井保博
  • Journal Title: 比較内分泌学 Volume: 36(138) Pages: 217-21
  • NAID: 10026666291
• [Presentation] Application of infrared laser to the zebrafish vascular system: gene induction, tracing, and ablation of single endothelial cells (2012)
  • Author(s): Kimura, E.,Deguchi, T., Kamei, Y., Shoji, W., Yuba, S., Hitomi, J
  • Organizer: 第35回分子生物学会年会博多
  • Place of Presentation: 博多
  • Year and Date: 2012-12-12
• [Presentation] Interactionbetween lymphatic vasculature and peripheral nerves during development of medaka (2012)
  • Author(s): Deguchi, T., Isogai, S., Shimoda, H.,Kawasaki, T., Yuba, S
  • Organizer: International Symposium 2012 Neuro-Vascular ring
  • Place of Presentation: Nara
  • Year and Date: 2012-11-12
• [Presentation] Medaka is a new animal model to study lymphatic development and regeneration (2012)
  • Author(s): Deguchi, T., Kawasaki, T., Yuba, S., Isogai, S., Shimoda, H., Nonaka, S., Hajiura-Kobayashi, H
  • Organizer: 18th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: Kyoto
  • Year and Date: 2012-09-22
• [Presentation] Lymphatic Development of medaka, Oryzias latipes (2012)
  • Author(s): Deguchi, T., Kawasaki, T., Yuba, S., Isogai, S., Shimoda, H., Nonaka, S.,Hajiura-Kobayashi, H
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
  • Year and Date: 2012-09-13
• [Presentation] The lymphatic vessels are visible in vivo in a pFLT4-EGFP transgenicmedaka (2011)
  • Author(s): Deguchi, T., Fujimori, KE., Kawasaki, T., Maruyama, K., Yuba,S
  • Organizer: The 1st Strategic Meeting for Medaka Research
  • Place of Presentation: Okazaki
  • Year and Date: 2011-11-23
• [Presentation] The lymphatic vessels are visible in vivo in a pFLT4-EGFP transgenic medaka (2011)
  • Author(s): Deguchi, T., Fujimori, KE., Kawasaki, T., Maruyama, K., Yuba, S.
  • Organizer: The 1st Strategic Meeting for Medaka Research
  • Place of Presentation: Okazaki Conference Center, Aichi (Japan)
  • Year and Date: 2011-11-23
• [Presentation] Generation of the transgenic medaka for in vivo imaging of cell-cycle (2011)
  • Author(s): Deguchi, T., Matsumoto, T., Kawasaki, T., Yuba, S.,Kinoshita, M., Sakaue-Sawano, A., Miyawaki, A., Naruse,K
  • Organizer: 第44回日本発生生物学会大会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Presentation] Generation of the transgenic medaka for in vivo imaging of cell-cycle (2011)
  • Author(s): Deguchi, T., Matsumoto, T., Kawasaki, T., Yuba, S., Kinoshita, M., Sakaue-Sawano, A., Miyawaki, A., Naruse, K.
  • Organizer: 第44回日本発生生物学会大会
  • Place of Presentation: 沖縄コンベンションセンター、沖縄
  • Year and Date: 2011-05-20
• [Presentation] pFLT4-EGFP トランスジェニックメダカを用いた in vivoでのリンパ管発生観察 (2010)
  • Author(s): Deguchi, T., Kawasaki,T., Isogai, S., Jiro, H., Yuba, S
  • Organizer: 第43回日本発生生物学会大会
  • Place of Presentation: 京都
  • Year and Date: 2010-06-22
• [Presentation] Lymphatic Development of medaka, Oryzias latipes.
  • Author(s): Deguchi, T., Kawasaki, T., Yuba, S., Isogai, S., Shimoda, H., Nonaka, S., Hajiura-Kobayashi, H.
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪
• [Presentation] Medaka is a new animal model to study lymphatic development and regeneration.
  • Author(s): Deguchi, T., Kawasaki, T., Yuba, S., Isogai, S., Shimoda, H., Nonaka, S., Hajiura-Kobayashi, H.
  • Organizer: 18th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 京都
• [Presentation] Interaction between lymphatic vasculature and peripheral nerves during development of medaka.
  • Author(s): Deguchi, T., Isogai, S., Shimoda, H., Kawasaki, T., Yuba, S.
  • Organizer: International Symposium 2012 Neuro-Vascular Wiring
  • Place of Presentation: 奈良
• [Presentation] Application of infrared laser to the zebrafish vascular system: gene inductio n, tracing, and ablation of single endothelial cells.
  • Author(s): Kimura, E., Deguchi, T., Kamei, Y., Shoji, W., Yuba, S., Hitomi, J.
  • Organizer: 第35回分子生物学会年会
  • Place of Presentation: 博多
• [Remarks]
  • URL: http://unit.aist.go.jp/hri/group/hri-terg/
• [Patent(Industrial Property Rights)] リンパ管可視化トランスジェニックメダカ及び該メダカを用いたリンパ管新生阻害薬のスクリーニング法 (2012)
  • Inventor(s): 出口 友則・藤森 一浩・川崎 隆史・弓場 俊輔
  • Industrial Property Rights Holder: 独立行政法人産業技術総合研究所
  • Acquisition Date: 2012-10-19