Comprehensive identification of caspase-dependent signal transduction pathway using cell-free protein array

• Project/Area Number: 22310127
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: System genome science
• Research Institution: Ehime University
• Principal Investigator: SAWASAKI Tatsuya 愛媛大学, 無細胞生命科学工学研究センター, 教授 (50314969)
• Co-Investigator(Kenkyū-buntansha): 酒巻 和弘 京都大学, 生命科学研究科, 准教授 (20271017)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000); Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 蛋白質ネットワーク / カスパーゼ / 無細胞タンパク質合成 / プロテオリシス / タンパク質 / 切断 / プロテインアレイ / コムギ胚芽 / AlphaScreen / Alpha Screen / 無細胞蛋白質合成系 / カスパーゼ3 / 蛋白質代謝 / 細胞死 / プロテアーゼ / 蛋白ライブラリー / プロテインカイネース / 細胞内シグナル

Research Abstract

Using a cell-free protein array constructed by a wheat cell-free protein synthesis system, comprehensive screening of caspase-3-cleaved protein kinases was carried out. Newly 30 protein kinases were found as substrates of caspase-3. Almost of cleavage sites were located on N- or C-terminal region flaking with regulatory domain. Actually protein function of some substrates was regulated by the cleavage of caspse-3.

Research Products

• [Journal Article] Myosin phosphatase is inactivated by caspase-3 cleavage and phosphorylation of myosin phosphatase targeting subunit 1 during apoptosis (2013)
  • Author(s): Iwasaki T, Katayama T, Kohama K, Endo Y, Sawasaki T
  • Journal Title: Mol. Biol. Cell Volume: 24 Pages: 748-56
  • Peer Reviewed
• [Journal Article] Novel approach to identifying autoantibodies in rheumatoid synovitis with a biotinylated human autoantigen library and the enzyme-labeled antigen method (2013)
  • Author(s): Mizutani Y, Matsuoka K, Takeda H, Shiogama K, Inada KI, Hayakawa K, Yamada H, Miyazaki T, Sawasaki T, Endo Y, Tsutsumi Y.
  • Journal Title: J Immunol Methods Volume: 387 Pages: 57-70
  • Peer Reviewed
• [Journal Article] Interferon-Induced SCYL2 Limits Release of HIV-1 by Triggering PP2A-Mediated Dephosphorylation of the Viral Protein Vpu. (2012)
  • Author(s): Miyakawa K, Sawasaki T, Matsunaga S, Tokarev A, Quinn G, Kimura H, Nomaguchi M, Adachi A, Yamamoto N, Guatelli J, Ryo A
  • Journal Title: Sci Signal Volume: 5
  • Peer Reviewed
• [Journal Article] Stress-Inducible Caspase Substrate TRB3 Promotes Nuclear Translocation of Procaspase-3 (2012)
  • Author(s): Shimizu K, Takahama S, Endo Y, Sawasaki T
  • Journal Title: PLoS One Volume: 7
  • Peer Reviewed
• [Journal Article] Genome-wide biochemical analysis of Arabidopsis protein phosphatase using a wheat cell-free system. (2012)
  • Author(s): Takahashi H, Ozawa A, Nemoto K, Nozawa A, Seki M, Shinozaki K, Takeda H, Endo Y, Sawasaki T.
  • Journal Title: FEBS Lett. Volume: 586 Pages: 3134-3141
  • Peer Reviewed
• [Journal Article] The molecular mechanism of apoptosis upon caspase-8 activation: Quantitative experimental validation of a mathematical model (2012)
  • Author(s): Kominami K, Nakabayashi J, Nagai T, Tsujimura Y, Chiba K, Kimura H, Miyawaki A, Sawasaki T, Yokota H, Manabe N, Sakamaki K
  • Journal Title: Biochim Biophys Acta. Volume: 1823 Pages: 1825-1840
  • Peer Reviewed
• [Journal Article] Molecular and enzymatic characterization of XMRV protease by a cell-free proteolytic analysis. (2012)
  • Author(s): Matsunaga S, Sawasaki T, Ode H, Morishita R, Furukawa A, Sakuma R, Sugiura W, Sato H, Katahira M, Takaori-Kondo A, Yamamoto N, Ryo A
  • Journal Title: J Proteomics. Volume: 75 Pages: 4863-4873
  • Peer Reviewed
• [Journal Article] novel Sec14 phospholipid transfer protein from Nicotiana benthamiana is up-regulated in response to Ralstonia solanacearum infection, pathogen associated molecular patterns and effector molecules and involved in plant immunity. (2012)
  • Author(s): Kiba A, Nakano M, Vincent-Pope P, Takahashi H, Sawasaki T, Endo Y, Ohnishi K, Yoshioka H, Hikichi Y. A
  • Journal Title: J Plant Physiol Volume: 169 Pages: 1017-1022
  • Peer Reviewed
• [Journal Article] In vivo imaging of hierarchical spatiotemporal activation of caspase-8 during apoptosis. (2012)
  • Author(s): Kominami K., et al.,
  • Journal Title: PLoS One. Volume: 7 Issue: 11 Pages: 1-18
  • DOI: 10.1371/journal.pone.0050218
  • Peer Reviewed
• [Journal Article] Establishment of a robust dengue virus NS3-NS5 binding assay for identification of protein-protein interaction inhibitors. (2012)
  • Author(s): Takahashi H., et al.,
  • Journal Title: Antiviral Res. Volume: 96 Issue: 3 Pages: 305-314
  • DOI: 10.1016/j.antiviral.2012.09.023
  • Peer Reviewed
• [Journal Article] Interferon-induced SCYL2 limits release of HIV-1 by triggering PP2A-mediated dephosphorylation of the viral protein Vpu. (2012)
  • Author(s): Miyakawa K., et al.,
  • Journal Title: Sci Signal. Volume: 5 Issue: 245
  • DOI: 10.1126/scisignal.2003212
  • Peer Reviewed
• [Journal Article] Stress-inducible caspase substrate TRB3 promotes nuclear translocation of procaspase-3. (2012)
  • Author(s): Shimizu K., et al.,
  • Journal Title: PLoS One. Volume: 7 Issue: 8 Pages: e42721-e42721
  • DOI: 10.1371/journal.pone.0042721
  • Peer Reviewed
• [Journal Article] The molecular mechanism of apoptosis upon caspase-8 activation: quantitative experimental validation of a mathematical model. (2012)
  • Author(s): Kominami K., et al.,
  • Journal Title: Biochim Biophys Acta. Volume: 1823 Issue: 10 Pages: 1825-1840
  • DOI: 10.1016/j.bbamcr.2012.07.003
  • Peer Reviewed
• [Journal Article] A novel Sec14 phospholipid transfer protein from Nicotiana benthamiana is up-regulated in response to Ralstonia solanacearum infection, pathogen associated molecular patterns and effector molecules and involved in plant immunity. (2012)
  • Author(s): Kiba A., et al.,
  • Journal Title: J Plant Physiol. Volume: 169 Issue: 10 Pages: 1017-1022
  • DOI: 10.1016/j.jplph.2012.04.002
  • Peer Reviewed
• [Journal Article] Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system. (2011)
  • Author(s): Nozawa A, Ogasawara T, Matsunaga S, Iwasaki T, Sawasaki T, Endo Y
  • Journal Title: BMC Biotechnol Volume: 11 Pages: 35-35
  • Peer Reviewed
• [Journal Article] Caspase-8 cleavage of the interleukin-21 (IL-21) receptor is a negative feedback regulator of IL-21 signaling. (2011)
  • Author(s): Akagi T, Shimizu K, Takahama S, Iwasaki T, Sakamaki K, Endo Y, Sawasaki T
  • Journal Title: FEBS Lett. Volume: 585 Pages: 1835-1840
  • Peer Reviewed
• [Journal Article] Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system. (2011)
  • Author(s): Nemoto K, Seto T, Takahashi H, Nozawa A, Seki M, Shinozaki K, Endo Y, Sawasaki T.
  • Journal Title: Phytochemistry Volume: 72 Pages: 1136-1144
  • Peer Reviewed
• [Journal Article] Specific In Situ Visualization of Plasma Cells Producing Antibodies Against Porphyromonas Gingivalis in Gingival Radicular Cyst: Application of the Enzyme-Labeled Antigen Method. (2011)
  • Author(s): Tsuge S, Mizutani Y, Matsuoka K, Sawasaki T, Endo Y, Naruishi K, Maeda H, Takashiba S, Shiogama K, Inada KI, Tsutsumi Y.
  • Journal Title: J Histochem Cytochem Volume: 59 Pages: 673-689
  • Peer Reviewed
• [Journal Article] Use of domain enzymes from wheat RNA ligase for in vitro preparation of RNA molecules. (2011)
  • Author(s): Makino S, Sawasaki T, Endo Y, Takai K
  • Journal Title: Biochem Biophys ResCommun Volume: 404 Pages: 1050-1054
  • Peer Reviewed
• [Journal Article] Specific in situ visualization of plasma cells producing antibodies against Porphyromonas gingivalis in gingival radicular cyst : application of the enzyme-labeled antigen method (2011)
  • Author(s): Tsuge S, et al.
  • Journal Title: J Histochem Cytochem Volume: 59 Pages: 673-689
  • Peer Reviewed
• [Journal Article] Caspase-8 cleavage of the interleukin-21 (IL-21) receptor is a negative feedback regulator of IL-21 signaling (2011)
  • Author(s): Akagi T.
  • Journal Title: FEBS Lett Volume: 585 Pages: 1835-1840
  • Peer Reviewed
• [Journal Article] Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system (2011)
  • Author(s): Nozawa A., et al.
  • Journal Title: BMC Biotechnol Volume: 11 Issue: 1 Pages: 35-35
  • DOI: 10.1186/1472-6750-11-35
  • Peer Reviewed
• [Journal Article] Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system (2011)
  • Author(s): Nemoto K., et al.
  • Journal Title: Phytochemistry Volume: 72 Pages: 1136-1144
  • Peer Reviewed
• [Journal Article] Use of domain enzymes from wheat RNA ligase for in vitro preparation of RNA molecules. (2011)
  • Author(s): Makino S, et al.
  • Journal Title: Biochem Biophys Res Commun. Volume: 404 Pages: 1050-1054
  • Peer Reviewed
• [Journal Article] Wheat germ cell-free protein production system for post-genomic research. (2011)
  • Author(s): Madono M, et al.
  • Journal Title: N Biotechnol. Volume: 28 Pages: 211-217
  • Peer Reviewed
• [Journal Article] Characterization of a caspase-3-substrate kinome using an N- and C-terminally tagged protein kinase library produced by a cell-free system. (2010)
  • Author(s): Tadokoro D, Takahama S, Shimizu K, Hayashi S, Endo Y, Sawasaki T.
  • Journal Title: Cell death & Dis Volume: 1
  • Peer Reviewed
• [Journal Article] Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis. (2010)
  • Author(s): Matsuoka K, Komori H, Nose M, Endo Y, Sawasaki T.
  • Journal Title: J Proteome Res. Volume: 9 Pages: 4264-4273
  • Peer Reviewed
• [Journal Article] In vitro dissection revealed that the kinase domain of wheat RNA ligase is physically isolatable from the flanking domains as a non-overlapping domain enzyme. (2010)
  • Author(s): Makino S, Sawasaki T, Endo Y, Takai K
  • Journal Title: Biochem Biophys Res Commun Volume: 397 Pages: 762-726
  • Peer Reviewed
• [Journal Article] Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins. (2010)
  • Author(s): Matsunaga S, Matsuoka K, Shimizu K, Endo Y, Sawasaki T.
  • Journal Title: BMC Biotechnol Volume: 10 Pages: 42-42
  • Peer Reviewed
• [Journal Article] Practical cell-free protein synthesis system using purified wheat embryos. (2010)
  • Author(s): Takai K, Sawasaki T, Endo Y.
  • Journal Title: Nat Protoc Volume: 5 Pages: 227-238
  • Peer Reviewed
• [Journal Article] Evaluating the role of rheumatoid factors for the development of rheumatoid arthritis in a mouse model with a newly established ELISA system. (2010)
  • Author(s): Tanaka Y, Komori H, Mori S, Soga Y, Tsubaki T, Terada M, Miyazaki T, Fujino T, Nakamura S, Kanno H, Sawasaki T, Endo Y, Nose M
  • Journal Title: Tohoku J Exp Med. Volume: 220 Pages: 199-206
  • NAID: 10027083539
  • Peer Reviewed
• [Journal Article] Wheat germ cell-free protein production system for post-genomic research. (2010)
  • Author(s): Madono M, Sawasaki T, Morishita R, Endo Y.
  • Journal Title: N Biotechnol. Volume: 28 Pages: 211-217
• [Journal Article] The Wheat-Germ Cell-Free Expression System. (2010)
  • Author(s): Takai K, Sawasaki T, Endo Y.
  • Journal Title: Curr Pharm Biotechnol. Volume: 11 Pages: 272-278
  • Peer Reviewed
• [Journal Article] Cell-free-based protein microarray technology using agarose/DNA microplate (2010)
  • Author(s): Sawasaki T, Endo Y.
  • Journal Title: Methods Mol Biol. Volume: 607 Pages: 63-72
• [Journal Article] An efficient approach to the production of vaccines against the malaria parasite. (2010)
  • Author(s): Tsuboi T, Takeo S, Sawasaki T, Torii M, Endo Y
  • Journal Title: Methods Mol Biol Volume: 607 Pages: 73-83
• [Journal Article] Cell-free protein synthesis for structure determination by X-ray crystallography. (2010)
  • Author(s): Watanabe M, Miyazono K, Tanokura M, Sawasaki T, Endo Y, Kobayashi I.
  • Journal Title: Methods Mol Biol Volume: 607 Pages: 149-160
• [Journal Article] Characterization of a caspase-3-substrate kinome using an N- and C-terminally tagged protein kinase library produced by a cell-free system. (2010)
  • Author(s): Tadokoro D, et al.
  • Journal Title: Cell death & Dis. Volume: 1
  • Peer Reviewed
• [Journal Article] In vitro dissection revealed that the kinase domain of wheat RNA ligase is physically isolatable from the flanking domains as a non-overlapping domain enzyme. (2010)
  • Author(s): Makino S, et al.
  • Journal Title: Biochem Biophys Res Commun. Volume: 397 Pages: 762-726
  • Peer Reviewed
• [Journal Article] Biotinylated-sortase self-cleavage purification (BISOP) method for cell-free produced proteins. (2010)
  • Author(s): Matsunaga S, et al.
  • Journal Title: BMC Biotechnol. Volume: 10 Pages: 42-42
  • Peer Reviewed
• [Journal Article] Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling. (2010)
  • Author(s): Kanchiswamy CN, et al.
  • Journal Title: BMC Plant Biol. Volume: 10 Pages: 97-97
  • Peer Reviewed
• [Journal Article] Practical cell-free protein synthesis system using purified wheat embryos. (2010)
  • Author(s): Takai K, et al.
  • Journal Title: Nat Protoc. Volume: 5 Pages: 227-238
• [Journal Article] Characterization of a caspase-3-substrate kinome using an N- and C-terminally tagged protein kinase library produced by a cell-free system. (2010)
  • Author(s): Sawasaki T, et al.
  • Journal Title: Methods Mol Biol. Volume: 607 Pages: 63-72
• [Journal Article] An efficient approach to the production of vaccines against the malaria parasite. (2010)
  • Author(s): Tsuboi T, et al.
  • Journal Title: Methods Mol Biol. Volume: 607 Pages: 73-83
• [Journal Article] Cell-free protein synthesis for structure determination by X-ray crystallography. (2010)
  • Author(s): Watanabe M, et al.
  • Journal Title: Methods Mol Biol. Volume: 607 Pages: 149-160
• [Journal Article] Arabidopsis CPK3 plays extensive roles in various biological and environmental responses. (2010)
  • Author(s): Arimura GI., Sawasaki T.
  • Journal Title: Plant Signal Behav. Volume: 5 Pages: 1263-1265
• [Presentation] シロイヌナズナキナーゼの基質探索技術 (2013)
  • Author(s): 根本 圭一郎ら
  • Organizer: 第５４回日本植物生理学会年会
  • Place of Presentation: 岡山大学 津島キャンパス（岡山市）
  • Invited
• [Presentation] 無細胞技術を基盤とした蛋白質ライブラリー構築 (2013)
  • Author(s): 竹田 浩之ら
  • Organizer: 第５４回日本植物生理学会年会
  • Place of Presentation: 岡山大学 津島キャンパス（岡山市）
  • Invited
• [Presentation] Identification and Functional Analysis of Tyrosine Phosphorylationdependent MAP Kinase Cascade (2012)
  • Author(s): 根本圭一郎, ら
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都国際会議場(京都市)
  • Year and Date: 2012-03-17
• [Presentation] Analysis of Caspase-dependent Signaling Mechanism on Myogenic Differentiation. (2012)
  • Author(s): 清水 康平ら
  • Organizer: 2012 ASCB Annual Meeting
  • Place of Presentation: Moscone Center, San Francisco, USA
• [Presentation] コムギ無細胞系発現抗原を用いた抗GPCRモノクローナル抗体の作製と評価 (2012)
  • Author(s): 小原 賢大ら
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡市）
• [Presentation] HCV タンパク質と相互作用するE3 リガーゼの網羅的探索 (2012)
  • Author(s): 黒河 健太ら
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡市）
• [Presentation] コムギ無細胞系を基盤としたLKB １をユビキチン化するE3 リガーゼの探索 (2012)
  • Author(s): 西森 香織ら
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡市）
• [Presentation] コムギ無細胞系を基盤としたp53 をユビキチン化するE3 リガーゼの同定 (2012)
  • Author(s): 安岡 左起ら
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡市）
• [Presentation] コムギ無細胞系を用いたCYLD と相互作用するE3 リガーゼの探索 (2012)
  • Author(s): 上松 篤史ら
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡市）
• [Presentation] Tyrosine-autophosphorylation protein kinases in Arabidopsis (2011)
  • Author(s): 根本圭一郎, ら
  • Organizer: Plant Genomes & Biotechnology : from Genes to Networks
  • Place of Presentation: Cold Spring Harbor Laboratory, USA
  • Year and Date: 2011-12-03
• [Presentation] Absolute quantitative analysis of mouse transmebrane proteome using a combination of wheat cell-free synthesis and MRM mass spectrometry (2011)
  • Author(s): Nobuaki Takemori, Ayako Takemori, Kazuhiro Matsuoka, Natsuki Matsushita, Hiroyuki Takeda, Tatsuya Sawasaki, Yaeta Endo and Shigeki Higashiyama
  • Organizer: プロテインアイランド松山国際シンポジウム 2011
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2011-09-22
• [Presentation] Kinome-wide screening reveals Sgk495 as a novel HCV NS3 substrate (2011)
  • Author(s): Shoukichi Takahama, Masaru Arimoto, Saki Miyajima, Takahiro Masaki, Keiichirou Nemoto, Takanobu Kato, Takaji Wakita, Tetsuro Suzuki, Yaeta Endo and Tatsuya Sawasaki
  • Organizer: プロテインアイランド松山国際シンポジウム2011
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2011-09-22
• [Presentation] コムギ無細胞タンパク質発現系を用いた植物チロシンキナーゼ(PPTK)の網羅的探索・同定および機能解析 (2011)
  • Author(s): 根本圭一郎, 関原明, 篠崎一雄, 遠藤弥重太, 澤崎 達也
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大
• [Presentation] Mechanical Regulation and Implication of Cancer: Oncogene-Induced Cell Transformation Involves Proteolysis of the Mechano-Sensing Protein p130Cas (2011)
  • Author(s): Keiko Kawauchi, Tan Song Hui, Gao Rong, Guo Alvin, Katsuhiko Iwasaki, Ichiro Harada, Hirotaka Takahashi, Tatsuya Sawasaki, Yaeta Endo, Yasuhiro Sawada
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学
• [Presentation] Caspase cleavage of ER stress-inducible TRB3 is a key switch from survival to cell death (2011)
  • Author(s): Kouhei Shimizu,Shoukichi Takahama, Tatsuya Sawasaki and Yaeta Endo
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学
• [Presentation] An attempt to prepare membrane proteins using the wheat cell-free protein production system (2011)
  • Author(s): Yaeta Endo, Tatsuya Sawasaki, Lassale Michael W.
  • Organizer: XXII International Congress and General Assembly of the IUCr
  • Place of Presentation: Municipal Conference Centre(Spain)
• [Presentation] AN ABSOLUTE QUANTIFICATION METHOD OF MOUSE TRANSMEMBRANE PROTEOME USING A COMBINATION OF WHEAT CELL-FREE SYNTHESIS AND MRM MASS SPECTROMETRY (2011)
  • Author(s): N. Takemori, A. Takemori, K. Matsuoka, N. Matsushita, T. Sawasaki, Y. Endo, S. Higashiyama
  • Organizer: HUPO 2011
  • Place of Presentation: Geneva Palexpo(Switzerland)
• [Presentation] IMMUNE EVASION BY HIV-1PROTEASE-MEDIATED CLEAVAGE OF TANK-BINDING KINASE 1 (2011)
  • Author(s): Tomohiro Kanuma, Ayumi Kudoh, Nao Jounai, Fumihiko Takeshita, Tatsuya Sawasaki, Akihide Ryo
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター、札幌市産業振興センター
• [Presentation] I CONSTRUCTION OF INFLUENZA VIRUS-LIKE PARTICLES USING A LIPOSOME-SUPPLEMENTED WHEAT CELL-FREE TRANSLATION SYSTEM (2011)
  • Author(s): Atsushi Muroi, Tomio Ogasawara, Kyoko Shinya, Akiko Makino, Teridah E Ginting, Yaeta Endo, Tatsuya Sawasaki
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター、札幌市産業振興センター
• [Presentation] AN IN VITRO CLEAVAGE ASSAY SYSTEM FOR XMRV PROTEASE BY WHEAT-GERM CELL FREE PROTEIN PRODUCTIO (2011)
  • Author(s): Satoko Matsunaga, Ayako Furukawa, Yoshitsugu Kojima, Ryo Morishita, Tatsuya Sawasaki, Akifumi Takaori-Konda, Wataru Sugiura, Masato Katahira, Akihide Ryo
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター、札幌市産業振興センター
• [Presentation] Regulation of eEF2K activity in Fas-induced apoptosis (2011)
  • Author(s): 岩崎隆宏, ら
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜国際会議場(横浜市)
• [Presentation] 無細胞基盤プロテオリポソームを用いた細胞への膜蛋白質導入技術の開発 (2011)
  • Author(s): 高橋守, ら
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜国際会議場(横浜市)
• [Presentation] カスパーゼ3,6,7により切断されるプロテインキナーゼの比較 (2011)
  • Author(s): 林祥太, ら
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜国際会議場(横浜市)
• [Presentation] コムギ無細胞系を基盤としたp53をユビキチン化するE3リガーゼの同定 (2011)
  • Author(s): 安岡佐起, ら
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜国際会議場(横浜市)
• [Presentation] 澤崎達也,遠藤弥重太.コムギ無細胞系発現プロテオリポソームを用いた抗膜タンパク質抗体作製 (2011)
  • Author(s): 竹田浩之, ら
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜国際会議場(横浜市)
• [Presentation] コムギ無細胞系基盤タンパク質ライブラリーを用いた新規乳がんマーカー探索 (2011)
  • Author(s): 小原賢大, ら
  • Organizer: 第6回無細胞生命科学研究会
  • Place of Presentation: 兵庫県立大学(姫路市)
• [Presentation] コムギ無細胞系発現プロテオリポソームを用いた抗膜タンパク質抗体作製 (2011)
  • Author(s): 竹田浩之, ら
  • Organizer: 第6回無細胞生命科学研究会
  • Place of Presentation: 兵庫県立大学(姫路市)
• [Presentation] CONSTRUCTION OF INFLUENZA VIRUS-LIKE PARTICLES USING A LIPOSOME-SUPPLEMENTED WHEAT CELL-FREE TRANSLATION SYSTEM (2011)
  • Author(s): 室井敦, ら
  • Organizer: IUMS2011
  • Place of Presentation: 札幌コンベンションセンター(札幌市)
• [Presentation] コムギ無細胞系を基盤としたがん化促進ユビキチン化E3リガーゼの構築 (2010)
  • Author(s): 安岡左起, ら
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸国際会議場(神戸市)(口頭およびポスター発表)
  • Year and Date: 2010-12-10
• [Presentation] Caspase-3により切断されるプロテインカイネースの比較 (2010)
  • Author(s): 林祥太, ら
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸国際会議場(神戸市)(ポスター発表)
  • Year and Date: 2010-12-09
• [Presentation] Caspase-3 cleavage of stress-responsible TRB3 attenuates activated Akt and activates caspase-3 activity (2010)
  • Author(s): 清水康平, ら
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸国際会議場(神戸市)(口頭およびポスター発表)
  • Year and Date: 2010-12-09
• [Presentation] 無細胞基質プロテオリポソームを用いた細胞への膜タンパク質導入技術の開発 (2010)
  • Author(s): 高橋守, ら
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸国際会議場(神戸市)(ポスター発表)
  • Year and Date: 2010-12-07
• [Presentation] 無細胞系を用いた機能プロテオミクス技術とウイルス研究への応用 (2010)
  • Author(s): 澤崎達也
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 徳島県郷土文化会館
  • Year and Date: 2010-11-08
• [Presentation] 無細胞基盤プロテオリポソームを用いた細胞への膜タンパク質導入技術の開発 (2010)
  • Author(s): 高橋守, 宮島早紀, 小笠原富夫, 遠藤弥重太, 澤崎 達也
  • Organizer: 第5回無細胞生命科学研究会
  • Place of Presentation: 岡山大学創立五十周年記念館
  • Year and Date: 2010-09-29
• [Presentation] Caspase-3,6,7 により切断されるプロテインカイネースの比較 (2010)
  • Author(s): 林祥太, 清水康平, 橋本季明, 吉川潮, 鎌田真司, 遠藤弥重太, 澤崎 達也
  • Organizer: 第5回無細胞生命科学研究会
  • Place of Presentation: 岡山大学創立五十周年記念館
  • Year and Date: 2010-09-29
• [Presentation] 植物 Protein Kinase ライブラリーを用いたPlant Protein Tyrosine Kinase の探索と同定 (2010)
  • Author(s): 根本圭一郎, 瀬藤拓也, 関原明, 篠崎一雄, 遠藤弥重太, 澤崎 達也
  • Organizer: 第5回無細胞生命科学研究会
  • Place of Presentation: 岡山大学創立五十周年記念館
  • Year and Date: 2010-09-29
• [Presentation] コムギ無細胞系を基盤としたがん化促進ユビキチン化 E3 リガーゼの探索 (2010)
  • Author(s): 安岡佐起, 遠藤弥重太, 澤崎 達也
  • Organizer: 第5回無細胞生命科学研究会
  • Place of Presentation: 岡山大学創立五十周年記念館
  • Year and Date: 2010-09-29
• [Presentation] 無細胞タンパク質合成系を用いた植物のカルシウム依存リン酸化制御機構の解明 (2010)
  • Author(s): 有村源一郎, Chidananda Nagamangala Kanchiswam, 高橋宏隆, 吉岡博文, 遠藤弥重太, 澤崎 達也
  • Organizer: 第5回無細胞生命科学研究会
  • Place of Presentation: 岡山大学創立五十周年記念館
  • Year and Date: 2010-09-29
• [Presentation] Development of cell-free based proteoliposome technology for delivering membrane proteins to cells (2010)
  • Author(s): Mamoru Takahashi, Saki Miyajima, Tomio Ogasawara, Yaeta Endo, Tatsuya Sawasaki
  • Organizer: プロテインアイランド松山国際シンポジウム 2010
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2010-09-28
• [Presentation] Screening of cancer-related E3 ubiquitin ligases by wheat cell-free system (2010)
  • Author(s): Saki Yasuoka, Yaeta Endo, Tatsuya Sawasaki
  • Organizer: プロテインアイランド松山国際シンポジウム 2010
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2010-09-28
• [Presentation] Autophosphorylation profiling of Arabidopsis protein kinases using the cell-free system (2010)
  • Author(s): Keiichirou Nemoto, Takuya Seto, Motoaki Seki, Kazuo Shinozaki, Yaeta Endo and Tatsuya Sawasaki
  • Organizer: プロテインアイランド松山国際シンポジウム 2010
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2010-09-28
• [Presentation] Downregulation of myosin phosphatase targeting subunit 1 during apoptosis (2010)
  • Author(s): K Takahiro Iwasaki, Yaeta Endo and Tatsuya Sawasaki
  • Organizer: プロテインアイランド松山国際シンポジウム 2010
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2010-09-28
• [Presentation] Biochemical comparison among of Caspase-3,6,7-cleaved protein kinases (2010)
  • Author(s): Shouta Hayashi, Kouhei imizu, Toshiaki Hashimoto, Ushio Kikkawa, Shinji Kamada, Yaeta Endo and Tatuya Sawasaki
  • Organizer: プロテインアイランド松山国際シンポジウム 2010
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2010-09-28
• [Presentation] Caspase-3 cleavage of stress-responsible TRB3 reduces Akt activity and increases caspase-3 activity (2010)
  • Author(s): Kouhei Shimizu, Shoukichi Takahama, Yaeta Endo and Tatsuya Sawasaki
  • Organizer: プロテインアイランド松山国際シンポジウム 2010
  • Place of Presentation: 松山全日空ホテル
  • Year and Date: 2010-09-28
• [Presentation] TRB3がcaspase-3に切断されるかどうかは細胞生存と細胞死のターニングポイントの一つである (2010)
  • Author(s): 清水康平, ら
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 大阪国際会議場(大阪市)(口頭およびポスター発表)
  • Year and Date: 2010-05-20
• [Presentation] アポトーシス時におけるミオシンホスファターゼ活性調節機構 (2010)
  • Author(s): 岩崎隆宏, ら
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 大阪国際会議場(大阪市)(ポスター発表)
  • Year and Date: 2010-05-20
• [Presentation] アポトーシス時におけるミオシンホスファターゼ活性調節機構 (2010)
  • Author(s): 岩崎 隆宏, 遠藤 弥重太, 澤崎 達也
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場
• [Presentation] TRB3 が caspase-3 に切断されるかどうかは細胞生存と細胞死のターニングポイントの一つである (2010)
  • Author(s): 清水 康平, 高濱 正吉, 遠藤 弥重太, 澤崎 達也
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場
• [Presentation] タンパク質ライブラリーを用いた自己抗体プロファイリング (2010)
  • Author(s): 澤崎 達也, 松岡和弘, 遠藤 弥重太
  • Organizer: 日本ヒトプロテオーム機構第8回大会
  • Place of Presentation: 東京ベイホテル東急
• [Presentation] 無細胞系を用いた機能プロテオミクス技術とウイルス研究への応用 (2010)
  • Author(s): 澤崎 達也, 遠藤 弥重太
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール
• [Presentation] 酵素活性を指標とした HIV プロテアーゼ薬剤耐性新規検査法の開発 (2010)
  • Author(s): 正岡 崇志, 杉浦 亙, 澤崎 達也, 松永 智子, 遠藤 弥重太, 巽 正志, Robert Shafer, 山本 直樹, 梁 明秀
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール
• [Presentation] HCV NS5A 蛋白のリン酸化に関与する新規セリン/ スレオニンプロテインキナーゼの同定と機能解析 (2010)
  • Author(s): 政木 隆博,松永 智子,高橋 宏隆,加藤 孝宣,遠藤 弥重太,澤崎 達也,脇田 隆字, 鈴木 哲朗
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール
• [Presentation] 無細胞基盤プロテオリポソームを用いた細胞への膜タンパク質導入技術の開発 (2010)
  • Author(s): 高橋守、宮島早紀、小笠原富夫、遠藤弥重太、澤崎 達也
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Regulation of Myosin Phosphatase during Apoptosis (2010)
  • Author(s): 岩崎隆宏,遠藤弥重太,澤崎 達也
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Caspase-3,6,7により切断されるプロテインカイネースの比較 (2010)
  • Author(s): 林祥太、清水康平、橋本季明、吉川潮、遠藤弥重太、澤崎 達也
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Caspase-3 によるストレス応答性TRB3 の切断は活性型 Akt を減衰しCaspase-3 を活性化する (2010)
  • Author(s): 清水康平、高濱正吉、遠藤弥重太、澤崎 達也
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] コムギ無細胞系を基盤としたがん化促進ユビキチン化 E3 リガーゼの探索 (2010)
  • Author(s): 安岡左起、佐々木敦朗、遠藤弥重太、澤崎 達也
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] 細胞死に伴う小胞形成の時系列的変動の解析 (2010)
  • Author(s): 酒巻和弘,坂本尚久,河村拓馬,千場久美子,澤崎 達也,小山田耕二
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Development of a Cell-Free Based Screening Method to Identify Cancer Specific Autoantigen Proteins (2010)
  • Author(s): K. Nishimori, K Matsuoka, Y. Endo, T. Sawasaki
  • Organizer: the 50th annual meeting the american society for cell biology
  • Place of Presentation: Pennsyllvania Convention Center (Philadelphia, Pennsyllvania, USA)
• [Presentation] Screening of Cancer-Related E3 Ubiquitin Ligase by Wheat Cell-Free System (2010)
  • Author(s): S. Yasuoka, Y. Endo, T. Sawasaki
  • Organizer: the 50th annual meeting the american society for cell biology
  • Place of Presentation: Pennsyllvania Convention Center (Philadelphia, Pennsyllvania, USA)
• [Presentation] Identification of a Host Factor Antagonizing Vpu-Mediated BST-2 /Tetherin Down-Regulation (2010)
  • Author(s): K. Miyakawa, T. Sawasaki, S. Matsunaga, N. Yamamoto, A. Ryo
  • Organizer: the 50th annual meeting the american society for cell biology
  • Place of Presentation: Pennsyllvania Convention Center (Philadelphia, Pennsyllvania, USA)
• [Presentation] An In Vitro Cleavage Assay System for XMRV Protease by Wheat-Germ Cell Free Protein Production. (2010)
  • Author(s): S. Matsunaga, Y. Kojima, R. Morishita, T. Sawasaki, A. Ryo
  • Organizer: the 50th annual meeting the american society for cell biology
  • Place of Presentation: Pennsyllvania Convention Center (Philadelphia, Pennsyllvania, USA)
• [Remarks]
  • URL: http://www.pros.ehime-u.ac.jp/cell-free
• [Remarks] 澤崎研究室
  • URL: http://www.pros.ehime-u.ac.jp/cell-free/
• [Remarks]
  • URL: http://www.ehime-u.ac.jp/~cellfree/sawasakilab/
• [Remarks]
  • URL: http://www.ehime-u.ac.jp/~cellfree/endolab/