The final form for highly sensitive and non-labeling separation-detection of non- or less-fluorescent chemicals in liquid solutions

• Project/Area Number: 22350035
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kyushu University
• Principal Investigator: HARATA Akira 九州大学, 総合理工学研究科(研究院), 教授 (90222231)
• Co-Investigator(Renkei-kenkyūsha): ISODA Miki 九州大学, 大学院・総合理工学府, 技術専門職員 (70467885)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 分析科学 / １分子計測 / 非蛍光性化学種 / 紫外レーザー分光 / 光熱変換 / ミクロ液体クロマトグラフィー / ヘテロダイン検出 / 生体・環境関連化学種 / 一分子計測 / ミクロクロマトグラフィー / ヘテロダイン計測 / 多環芳香族 / 1分子計測 / 親水性相互作用

Research Abstract

As an ultimate method for highly sensitive and non-labeling separation-detection of non- or less-fluorescent chemical species in liquid solutions, a new equipment is developed in coupling micro-liquid chromatographic separation with the heterodyne interferometic detection of ultraviolet-laser excited photothermal effects. Photothermal signal is successfully detected with heterodyne interferometer for liquid sample in micro-flow cells. Separation condition is assigned for non-labeled 20 kinds of biologically important amino acids.

Research Products

• [Journal Article] 溶液中にある非蛍光性化学種の単一分子検出向けて (2013)
  • Author(s): 原田 明
  • Journal Title: Toward single molecule detection of non-fluorescent species in solution, 信学技報 (IEICE Technical Report) Volume: Vol.113, No.167 Pages: 51-54
• [Journal Article] 溶液中にある非蛍光性化学種の単一分子検出向けて (2013)
  • Author(s): 原田 明
  • Journal Title: 信学技報 Volume: 113 Pages: 51-54
• [Journal Article] Development of the Multi-color Excitation Photothermal Lens Microscope (2012)
  • Author(s): Noriyuki FUJII and Akira HARATA
  • Journal Title: Proc. 33rd Symposium on Ultrasonic Electronics Pages: 331-332
  • Peer Reviewed
• [Journal Article] Rapid Decomposition of Cellulose Dissolved in Ionic Liquid Using Gas/Liquid Interface Discharge (2012)
  • Author(s): Shoichiro FURUKAWA
  • Journal Title: Japanese Journal of Applied Physics Volume: 51 Issue: 7R Pages: 0702051-3
  • DOI: 10.1143/jjap.51.070205
  • Peer Reviewed
• [Journal Article] Release of Nile Red from Thermoresponsive Gold Nanocomposites by Heating a Solution and the Addition of Glutathione (2012)
  • Author(s): Kentaro TOMITA
  • Journal Title: Analytical Sciences Volume: 28 Issue: 12 Pages: 1125-1132
  • DOI: 10.2116/analsci.28.1125
  • NAID: 130004442096
  • ISSN: 0910-6340, 1348-2246
  • Peer Reviewed
• [Journal Article] Ultraviolet laser-excited autofluorescence for aqueous solution of amino acids (2011)
  • Author(s): Takahiro ONIKI and Akira HARATA
  • Journal Title: Proc. 13rd Cross Straits Symposium on Materials, Energy and Environmental Sciences Pages: 207-208
• [Journal Article] Direct Detection of Polycyclic Aromatic Hydrocarbons by Using micro-HPLC/Ultraviolet Excitation Thermal Lens Spectroscopy (2011)
  • Author(s): Sayaka NOJI, Miki ISODA and Akira HARATA
  • Journal Title: Proc. of 13rd Cross Straits Symposium on Materials, Energy and Environmental Sciences Pages: 171-172
• [Journal Article] Observation of Single Yeast Cell with Advanced Photothermal Lens Microscope (2011)
  • Author(s): Noriyuki FUJII and Akira HARATA
  • Journal Title: Proceedings of the 32th Symposium on Ultrasonic Electronics Pages: 81-82
  • Peer Reviewed
• [Journal Article] Development of Reflection Objective-Employed Collinear Mode-mismatched Thermal Lens Microscope (2011)
  • Author(s): Noriyuki Fujii, et al
  • Journal Title: Japanese Journal of Applied Physics Volume: 50 Issue: 7S Pages: 07HC05-07HC05
  • DOI: 10.1143/jjap.50.07hc05
  • Peer Reviewed
• [Journal Article] Chemistry at the water surface : Spectroscopic investigation on behaviors of molecules in interfacial nano-environments (2011)
  • Author(s): Akira HARATA
  • Journal Title: Kyushu University G-COE program "NOVEL CARBON RESOURCE SCIENCES" Volume: 5 Pages: 19-23
  • NAID: 40018939445
• [Journal Article] Development of Photothermal Lens UV Imaging Method for Living Cells (2010)
  • Author(s): Noriyuki FUJII and Akira HARATA
  • Journal Title: Proceedings of the 31th Symposium on Ultrasonic Electronics Pages: 181-182
  • Peer Reviewed
• [Journal Article] Direct detection of gradient-eluted non-labeled amino acids using micro-HPLC with ultraviolet thermal lensing (2010)
  • Author(s): Hitoshi KATAE, Satoshi HIRASHIMA, and Akira HARATA
  • Journal Title: J. Phys. : Conf. Ser.214 Volume: 012122 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Direct detection of gradient-eluted non-labeled amino acids using micro-HPLC with ultraviolet thermal lensing (2010)
  • Author(s): Hitoshi KATAE, Satoshi HIRASHIMA, Akira HARATA
  • Journal Title: Journal of Physics : Conference Series Volume: 214
  • Peer Reviewed
• [Presentation] Ultrasensitive Detection of Nonfluorescent Molecules in Liquid Solutions (2013)
  • Author(s): Akira HARATA
  • Organizer: 15th Beijing conference and exhibition on Instrumental Analysis
  • Place of Presentation: Beijing, China
  • Year and Date: 2013-10-25
  • Invited
• [Presentation] 溶液中にある非蛍光性化学種の単一分子検出向けて (2013)
  • Author(s): 原田 明
  • Organizer: 第58回音波と物性討論会
  • Place of Presentation: 九州大学筑紫キャンパス, 福岡
  • Year and Date: 2013-07-29
  • Invited
• [Presentation] 多色励起光熱変換分光法の開発 (2013)
  • Author(s): 野崎 恭平, 原田 明
  • Organizer: 第50回化学関連支部合同九州大会
  • Place of Presentation: 小倉
  • Year and Date: 2013-07-06
• [Presentation] マイクロHPLCを用いた環境関連物質の微量成分分析 (2013)
  • Author(s): 松本 滉生, 原田 明
  • Organizer: 第50回化学関連支部合同九州大会
  • Place of Presentation: 小倉
  • Year and Date: 2013-07-06
• [Presentation] micro-HPLC/紫外励起熱レンズ法を用いた無標識化学種の分離高感度検出と溶媒 (2013)
  • Author(s): 原田 明, 井之上 紗緒梨
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 北海道大学函館キャンパス
  • Year and Date: 2013-05-18
• [Presentation] micro-HPLC/紫外励起熱レンズ法を用いた無標識化学種の分離高感度検出と溶媒 (2013)
  • Author(s): 原田明, 井之上紗緒梨
  • Organizer: 第73回分析化学討論会
  • Place of Presentation: 函館
• [Presentation] 紫外励起光熱変換顕微鏡によるミクロ粒子の観察 (2013)
  • Author(s): 眞子 翠、藤井宜行、原田 明
  • Organizer: 第31回九州分析化学若手の会夏季セミナー
  • Place of Presentation: 長崎
• [Presentation] ミクロHPLC/顕微ヘテロダイン干渉検出法による生体関連物質の高感度検出 (2013)
  • Author(s): 福間 誠、原田 明
  • Organizer: 第31回九州分析化学若手の会夏季セミナー
  • Place of Presentation: 長崎
• [Presentation] 溶液中にある非蛍光性化学種の単一分子検出向けて (2013)
  • Author(s): 原田 明
  • Organizer: 第58回音波と物性討論会
  • Place of Presentation: 福岡
  • Invited
• [Presentation] Development of the Multi-color Excitation Photothermal Lens Microscope (2012)
  • Author(s): Noriyuki FUJII and Akira HARATA
  • Organizer: 第33回超音波エレクトロニクスの基礎と応用に関する シンポジウム
  • Place of Presentation: 千葉大学
  • Year and Date: 2012-11-15
• [Presentation] Three Dimensional Imaging with Multi-color Excitation Photothermal Lens Microscope (2012)
  • Author(s): Noriyuki FUJII and Akira HARATA
  • Organizer: 9th International Symposium on NOVEL CARBON RESOURCE SCIENCES
  • Place of Presentation: 九大筑紫
  • Year and Date: 2012-11-03
• [Presentation] micro-HPLC/紫外励起熱レンズ法を用いた酸性および塩基性アミノ酸の無標識高感度分離検出 (2012)
  • Author(s): 井之上 紗緒梨, 原田 明
  • Organizer: 日本分析化学会第61年会
  • Place of Presentation: 金沢大学
  • Year and Date: 2012-09-20
• [Presentation] 反射対物型紫外励起熱レンズ顕微鏡による3次元イメージング (2012)
  • Author(s): 藤井 宣行, 原田 明
  • Organizer: 日本分析化学会第61年会
  • Place of Presentation: 金沢大学
  • Year and Date: 2012-09-19
• [Presentation] 酸性および塩基性アミノ酸の無標識高感度分離検出 (2012)
  • Author(s): 井之上 紗緒梨 , 原田 明
  • Organizer: 酸性および塩基性アミノ酸の無標識高感度分離検出
  • Place of Presentation: 小倉
  • Year and Date: 2012-06-30
• [Presentation] 深紫外レーザー励起による非蛍光性アミノ酸水溶液の蛍光スペ クトル測定 (2012)
  • Author(s): 原田 明, 鬼木 喬玄
  • Organizer: 第72回分析化学討論会(鹿児島大学郡元キャンパス)
  • Place of Presentation: 鹿児島
  • Year and Date: 2012-05-18
• [Presentation] Development of Multi-color Excitation Photothermal Lens Microscope (2012)
  • Author(s): Noriyuki Fujii
  • Organizer: 第33回超音波エレクトロニクスの基礎と応用に関するシンポジウム
  • Place of Presentation: 千葉
• [Presentation] 深紫外レーザー励起による非蛍光性アミノ酸水溶液の蛍光スペクトル測定 (2012)
  • Author(s): 鬼木 喬玄
  • Organizer: 第72回分析化学討論会
  • Place of Presentation: 鹿児島
• [Presentation] micro HPLC / 紫外励起熱レンズ分光法を用いた酸性および塩基性アミノ酸の無標識高感度分離検 (2012)
  • Author(s): 井之上 沙緒梨
  • Organizer: 日本分析化学会第61年会
  • Place of Presentation: 金沢
• [Presentation] Ultraviolet laser-excited autofluorescence for aqueous solution of amino acids (2011)
  • Author(s): T. ONIKI A. HARATA
  • Organizer: 13rd Cross Straits Symposium on Materials, Energy and Eenvironmental Sciences (CSS13)
  • Place of Presentation: Kasuga, Kyushu Univ., Fukuoka
  • Year and Date: 2011-11-23
• [Presentation] Direct Detection of Polycyclic Aromatic Hydrocarbons By Using micro-HPLC/Ultraviolet Excitation Thermal Lens Spectroscopy (2011)
  • Author(s): Sayaka NOJI, Miki ISODA, Akira HARATA
  • Organizer: 13rd Cross Straits Symposium on Materials, Energy and Environmental Sciences (CSS13)
  • Place of Presentation: Kasuga, Kyushu Univ., Fukuoka
  • Year and Date: 2011-11-23
• [Presentation] Direct Detection of Polycyclic Aromatic Hydrocarbons By Using micro-HPLC/Ultraviolet Excitation Thermal Lens Spectroscopy (2011)
  • Author(s): Sayaka Noji, Miki Isoda, Akira Harata
  • Organizer: The 13rd Cross Straits Symposium on Materials, Energy and environmental Sciences (CSS13)
  • Place of Presentation: 福岡
  • Year and Date: 2011-11-23
• [Presentation] Ultraviolet laser-excited autofluorescence for aqueous solution of amino acids (2011)
  • Author(s): T.Oniki, A.Harata
  • Organizer: The 13rd Cross Straits Symposium on Materials, Energy and environmental Sciences (CSS13)
  • Place of Presentation: 福岡
  • Year and Date: 2011-11-23
• [Presentation] Observation of Single Yeast Cell with Advanced Photothermal Lens (2011)
  • Author(s): Noriyuki FUJII and Akira HARATA
  • Organizer: 第32回超音波エレクトロニクスの基礎と応用に関するシンポジウム
  • Place of Presentation: 京都大学
  • Year and Date: 2011-11-09
• [Presentation] Observation of Single Yeast Cell with Advanced Photothermal Lens (2011)
  • Author(s): Noriyuki Fujii, Akira Harata
  • Organizer: 第32回超音波エレクトロニクスの基礎と応用に関するシンポジウム
  • Place of Presentation: 京都
  • Year and Date: 2011-11-08
• [Presentation] 細胞観察のための広波長域対応型紫外熱レンズイメージング法の開発 (2011)
  • Author(s): 藤井 宣行, 原田 明
  • Organizer: 2011年度総理工セミナー
  • Place of Presentation: 千里阪急ホテル,大阪
  • Year and Date: 2011-09-16
• [Presentation] micro-HPLC/紫外励起熱レンズ法を用いた多環芳香族炭化水素の分離検出 (2011)
  • Author(s): 野地 紗也加, 礒田 美紀, 原田 明
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2011-09-14
• [Presentation] 深紫外レーザー光照射時のアミノ酸の分光特性解析 (2011)
  • Author(s): 鬼木 喬玄, 原田 明
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2011-09-14
• [Presentation] 深紫外レーザー照射時のアミノ酸の分光特性解析 (2011)
  • Author(s): 鬼木喬玄、原田明
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-14
• [Presentation] micro-HPLC/紫外励起熱レンズ法を用いた多環芳香族炭化水素の分離検出 (2011)
  • Author(s): 野地紗也加、礒田美紀、原田明
  • Organizer: 日本分析化学会第60年会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-09-14
• [Presentation] 反射対物型紫外励起熱レンズ顕微鏡の開発と細胞イメージングへの適用 (2011)
  • Author(s): 藤井 宣行, 原田 明
  • Organizer: 第72回応用物理学会学術講演会
  • Place of Presentation: 山形大学
  • Year and Date: 2011-08-29
• [Presentation] 紫外励起熱レンズ法とmicro-HPLCを組み合わせた多環芳香族炭化水素の分離検出 (2011)
  • Author(s): 野地 紗也加, 礒田 美紀, 原田 明
  • Organizer: 第48回化学関連支部合同九州大会
  • Place of Presentation: 小倉
  • Year and Date: 2011-07-09
• [Presentation] 深紫外レーザー光を用いたアミノ酸の分光特性解析 (2011)
  • Author(s): 鬼木 喬玄, 原田 明
  • Organizer: 第48回化学関連支部合同九州大会
  • Place of Presentation: 小倉
  • Year and Date: 2011-07-06
• [Presentation] Design for Highly Sensitive Detection of Non-Labeled Amino Acids Using Hilic with Micro-HPLC/ Ultraviolet Thermal Lens Spectroscopy (2011)
  • Author(s): Yujiro SUMI and Akira HARATA
  • Organizer: IUPAC International Congress on Analytical Sciences 2011
  • Place of Presentation: 京都国際会議場, Kyoto
  • Year and Date: 2011-05-23
• [Presentation] Design for Highly Sensitive Detection of Non-Labeled Amino Acids Using Hilic with Micro-HPLC/Ultraviolet Thermal Lens Spectroscopy (2011)
  • Author(s): Yujiro Sumi, Akira Harata
  • Organizer: IUPAC International Congress on Analytical Sciences 2011
  • Place of Presentation: 京都
  • Year and Date: 2011-05-22
• [Presentation] Direct detection of nonlabeled amino acids using micro-HILICHPLC with ultraviolet thermal lens spectroscopy (2010)
  • Author(s): Y.Sumi, A.Hazata
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies (Pacifichem 2010)
  • Place of Presentation: Honolulu(米国)
  • Year and Date: 2010-12-19
• [Presentation] Direct detection of nonlabeled amino acids using micro-HILIC-HPLC with ultraviolet thermal lens spectroscopy (2010)
  • Author(s): Y. SUMI and A. HARATA
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies(Pacifichem 2010)
  • Place of Presentation: Honolulu, U.S.A.
  • Year and Date: 2010-12-18
• [Presentation] Ultraviolet photothermal image of a single nonstained yeast cell measured with reflection objective (2010)
  • Author(s): N. FUJII and A. HARATA
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies(Pacifichem 2010)
  • Place of Presentation: Honolulu, U.S.A.
  • Year and Date: 2010-12-17
• [Presentation] Development of Photothermal Lens UV Imaging Method for Living Cells (2010)
  • Author(s): Noriyuki FUJII and Akira HARATA
  • Organizer: 第31回超音波エレクトロニクスの基礎と応用に関するシンポジウム
  • Place of Presentation: 日本大・駿河台, 東京
  • Year and Date: 2010-12-06
• [Presentation] Development of Photothermal Lens UV Imaging Method for Living Cells (2010)
  • Author(s): N.Fujii, A.Harata
  • Organizer: 第31回 超音波エレクトロニクスの基礎と応用に関するシンポジウム
  • Place of Presentation: 日本大学(東京都)
  • Year and Date: 2010-12-06
• [Presentation] Technical Points of Photothermal Lens UV Imaging (2010)
  • Author(s): Noriyuki FUJII and Akira HARATA
  • Organizer: The 6th International Symposium on Novel Carbon Resource Sciences - Aiming toward Low-Carbon Society -
  • Place of Presentation: Kyushu University, Kasuga
  • Year and Date: 2010-11-12
• [Presentation] 紫外励起熱レンズ分光法/micro-HPLCを用いたHILICによる無標識アミノ酸の高感度分離分析 (2010)
  • Author(s): 角裕次郎, 原田 明
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 東北大, 仙台
  • Year and Date: 2010-09-15
• [Presentation] 紫外励起熱レンズ分光法/micro-HPLCを用いたHILICによる無標識アミノ酸の高感度分離分析 (2010)
  • Author(s): 角裕次郎、原田明
  • Organizer: 日本分析化学会第59年会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2010-09-15
• [Presentation] 紫外励起熱レンズ法とmicro-HILIC-HPLCによる無標識アミノ酸の高感度分離検出 (2010)
  • Author(s): 角裕次郎, 原田 明
  • Organizer: 第47回化学関連支部合同九州大会
  • Place of Presentation: 北九州国際会議場, 小倉
  • Year and Date: 2010-07-10
• [Presentation] Spectroscopic Investigation of Environmental Molecules at the Water Surface (2010)
  • Author(s): Akira HARATA, Yasushi IMNISHI, Miki SATO ( ISODA ) , and Toshio ISHIOKA
  • Organizer: 5th International Symposium on Novel Carbon Resource Sciences
  • Place of Presentation: Curtin University of Technology, Perth, Australia
  • Year and Date: 2010-04-22
• [Book] 化学のブレークスルー - 革新論文から見たこの10年の進歩と未来, 生細胞をターゲットとした顕微鏡が技術発展を加速する (2011)
  • Author(s): 原田 明
  • Total Pages: 293
  • Publisher: 化学同人編集部編
• [Book] 化学のブレークスルー【機器分析編】, 生細胞をターゲットとした顕微鏡が技術発展を加速する (2011)
  • Author(s): 原田 明
  • Total Pages: 82
  • Publisher: 別冊化学
• [Book] Ionization of Solute Molecules at the Liquid Water Surface, Interfaces, and Self-Assembled Systems (2010)
  • Author(s): Akira HARATA, Miki SATO ( ISODA ) , and Toshio ISHIOKA
• [Book] Charged Particle and Photon Interactions with Matter : Recent Advances, Applications, and Interfaces (2010)
  • Author(s): Akira Harata, Miki Sato, Toshio Ishioka
  • Total Pages: 1045
  • Publisher: CRC Press
• [Remarks] 「原田 明」教育研究関連のデータベース
  • URL: http://www.mm.kyushu-u.ac.jp/lab_07/members/harata/AHDB_01.html
• [Remarks] 「原田 明」教育研究関連のデータベース
  • URL: http://www.mm.kyushu-u.ac.jp/lab_07/members/harata/AHDB_01.html
• [Remarks] 「原田 明」教育研究関連のデータベース
  • URL: http://www.mm.kyushu-u.ac.jp/lab_07/members/harata/AHDB_01.html
• [Remarks]
  • URL: http://www.mm.kyushu-u.ac.jp/lab_07/members/harata/AHDB_01.html