Analysis of protozoa in the natural environment for bioaugmentation

• Project/Area Number: 22360216
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: Tohoku Gakuin University
• Principal Investigator: NAKAMURA Kanji 東北学院大学, 工学部, 教授 (90382655)
• Co-Investigator(Kenkyū-buntansha): ENDO Ginro 東北学院大学, 工学部, 環境建設工学科 (80194033) ; MIYAUCHI Keisuke 東北学院大学, 工学部, 環境建設工学科 (20324014)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
• Keywords: 環境浄化 / バイオオーグメンテーション / 捕食 / 原生動物 / 特定細菌 / バイオラセイン / 蛍光遺伝子 / 特定微生物 / real-time PCR / 18S rRNA / グラム陰性細菌 / グラム陽性細菌 / 被食者 / ITS

Research Abstract

Bioaugmentation is a method to use a special microorganism for soil cleanup. However, microorganisms introduced to a contaminated site decrease rapidly in many cases. In this research, we found out that the decrease was caused by protozoa existing in the natural environment. Most of protozoa that we have found were flagellates that were classified to Chrysophyceae, Stramenopiles. Several flagellates were isolated and they were found to ingest various kinds of bacteria.

Research Products

• [Journal Article] 自然環境中に生息する細菌捕食性原生動物の解析 (2012)
  • Author(s): 中村寛治,須藤真志
  • Journal Title: 土木学会論文集G(環境) Volume: Vol.68 Pages: 31-40
  • NAID: 130004962411
  • Peer Reviewed
• [Journal Article] Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2 (2012)
  • Author(s): Ikeda-Ohtsubo W, Miyahara M, Kim SW, Yamada T, Matsuoka M, Watanabe A, Fushinobu S, Wakagi T, Shoun H, Miyauchi K, Endo G
  • Journal Title: J Biosci Bioeng. Volume: Sep18
  • NAID: 110009578187
  • Peer Reviewed
• [Journal Article] 自然環境中に生息する細菌捕食性原生動物の解析 (2012)
  • Author(s): 中村寛治
  • Journal Title: 土木学会論文集G（環境） Volume: 68 Pages: 31-40
  • NAID: 130004962411
  • Peer Reviewed
• [Journal Article] 広瀬川河川水からのBacillus 属細菌捕食性原生動物の単離および解析 (2010)
  • Author(s): 須藤真志,中村寛治
  • Journal Title: 環境工学研究論文集 Volume: Vol.47 Pages: 85-91
  • Peer Reviewed
• [Journal Article] ビニルクロライドリダクターゼ遺伝子の取得・解析および浄化現場での検出 (2010)
  • Author(s): 中村寛治, 荒聡美, 水本正浩, 上野俊洋, 石田浩昭
  • Journal Title: 環境工学研究論文集 Volume: 47 Pages: 243-249
  • Peer Reviewed
• [Journal Article] 広瀬川河川水からのBacillus属細菌捕食性原生動物の単離および解析 (2010)
  • Author(s): 須藤真志, 中村寛治
  • Journal Title: 環境工学研究論文集 Volume: 47 Pages: 85-91
  • Peer Reviewed
• [Journal Article] 自然環境下に放出された特定細菌の挙動解析 (2010)
  • Author(s): 中村寛治
  • Journal Title: 環境バイオテクノロジー学会誌 Volume: 10 Pages: 59-62
  • Peer Reviewed
• [Presentation] トリクロロエチレン分解能を有する細菌への蛍光遺伝子の導入および検出 (2013)
  • Author(s): 渡辺健幸
  • Organizer: 土木学会 東北支部 技術研究発表会
  • Place of Presentation: 東北大学 川内キャンパス
• [Presentation] トリクロロエチレン分解能を有する細菌への蛍光遺伝子の導入および検出 (2012)
  • Author(s): 渡辺健幸・中村寛治
  • Organizer: 平成 24 年度 土木学会 東北支部 技術研究発表会
  • Place of Presentation: 東北大学 川内キャンパス
  • Year and Date: 2012-03-09
• [Presentation] 多賀城市内の水環境に生息する細菌捕食性原生動物の単離および解析 (2011)
  • Author(s): 千葉峻(中村研究室大学院生)
  • Organizer: 第48回環境工学研究フォーラム(土木学会)
  • Place of Presentation: 大同大学(愛知県)
  • Year and Date: 2011-11-26
• [Presentation] Analysis of indigenous protozoa in the natural environment (2011)
  • Author(s): K. Nakamura
  • Organizer: 111th General Meeting of the American Society for Microbiology
  • Place of Presentation: New OrleansConvention Center, Louisiana, USA
  • Year and Date: 2011-05-22
• [Presentation] Analysis of indigenous protozoa in a river that graze foreign bacteria introduced (2011)
  • Author(s): Kanji NAKAMURA
  • Organizer: 111th General Meeting of the American Society for Microbiology
  • Place of Presentation: New Orleans Convention Center, Louisiana, USA
  • Year and Date: 2011-05-22
• [Presentation] Analysis of indigenous protozoa in a river that graze foreign bacteria introduced (2010)
  • Author(s): K. Nakamura
  • Organizer: IBS 2010 The 14th International Biotechnology Symposium
  • Place of Presentation: Palacongressi, Rimini - Italy
  • Year and Date: 2010-09-14
• [Presentation] Analysis of indigenous protozoa in a river that graze foreign bacteria introduced (2010)
  • Author(s): Kanji Nakamura
  • Organizer: IBS 2010 The 14th International Biotechnology Symposium
  • Place of Presentation: Palacongressi, Rimini-Italy
  • Year and Date: 2010-09-14
• [Book] バイオ活用による汚染・廃水の新処理法 (2012)
  • Author(s): 中村寛治、他(共著)
  • Publisher: シー・エム・シー出版
• [Book] バイオ活用による汚染・廃水の新処理法 (2012)
  • Author(s): 中村寛治, 他(共著)
  • Total Pages: 9
  • Publisher: シー・エム・シー出版