Regulatory mechanisms of the auxin response mediated by nuclear-cytoplasmic transport systems

• Project/Area Number: 22370018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kobe University
• Principal Investigator: FUKAKI Hidehiro 神戸大学, 理学(系)研究科(研究院), 准教授 (80324979)
• Co-Investigator(Renkei-kenkyūsha): MIMURA Tetsuro 神戸大学, 大学院理学研究科, 教授 (20174120)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000); Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
• Keywords: 植物ホルモン / 遺伝子発現 / 核-細胞質間輸送 / シロイヌナズナ / マイクロRNA / 側根形成 / オーキシン / 核ー細胞質間輸送

Research Abstract

Auxin is one of the important hormones for growth and development in plants. In general, auxin-mediated growth and developmental processes are regulated by auxin synthesis, auxin transport and auxin-dependent gene expression. However, the mechanisms of auxin responses still remain unknown. Genetic analyses using Arabidopsis thaliana mutants defective in lateral root formation have shown that HASTY/exportin-5, a component of the nuclear pore complex, is involved in auxin-mediated lateral root formation. In this project, we found that both biosynthesis of microRNAs and nuclear-cytoplasmic transport are necessary for the regulation of SLR/IAA14-ARF7/19 module-dependent auxin response in lateral root formation.

Research Products

• [Journal Article] GNOM/FEWER ROOTS is required for the establishment of an auxin response maximum for Arabidopsis lateral root initiation (2013)
  • Author(s): Okumura K., Goh T., Toyokura K., Kasahara H., Takabayashi Y., Mimura T., Kamiya Y., and Fukaki H.
  • Journal Title: Plant Cell Physiol. Volume: 54 Pages: 406-417
  • NAID: 10031163836
  • Peer Reviewed
• [Journal Article] GNOM/FEWER ROOTS is required for the establishment of auxin response maximum for Arabidopsis lateral root initiation. (2013)
  • Author(s): Okumura, K. I., Goh, T., To yokura, K., Kasahara, H, T akabayashi, Y, Mimura, T., Kamiya Y. and Fukaki, H.
  • Journal Title: Plant and Cell Physiology Volume: 54 Issue: 3 Pages: 406-417
  • DOI: 10.1093/pcp/pct018
  • NAID: 10031163836
  • Peer Reviewed
• [Journal Article] Multiple Aux/IAA-ARF modules regulate lateral root formation : the role of Arabidopsis SHY2/IAA3-mediated auxin signaling (2012)
  • Author(s): Goh T., Kasahara H., Mimura T., Kamiya Y., and Fukaki H.
  • Journal Title: Phil. Trans. R. Soc. B Biol. Sci. Volume: 367 Pages: 1461-1468
  • Peer Reviewed
• [Journal Article] The establishment of asymmetry in Arabidopsis lateral root founder cells is regulated by LBD16/ASL18 and related LBD/ASL proteins (2012)
  • Author(s): Goh T., Joi S., Mimura T., and Fukaki H.
  • Journal Title: Development Volume: 139 Pages: 883-893
  • Peer Reviewed
• [Journal Article] Multiple Aux/IAA-ARF modules regulate lateral root formatio n : the role of Arabidopsis SITY2/IAA3-mediated auxin signaling. (2012)
  • Author(s): Goh, T., Kasahara H., Mim ura, T., Kamiya Y. and Fu kaki, H.
  • Journal Title: Phil. Trans. R. Soc. B Biol. Sci. Volume: 367 Issue: 1595 Pages: 1461-1468
  • DOI: 10.1098/rstb.2011.0232
  • Peer Reviewed
• [Journal Article] オーキシンを介した側根形成の分子機構 (2012)
  • Author(s): 深城英弘、 郷達明
  • Journal Title: 植物の生長調節 Volume: 47 Pages: 93-100
• [Journal Article] The establishment of asymmetry in Arabidopsis lateral root founder cells is regulated by LBD16/ASL18 and related LBD/ASL proteins. (2012)
  • Author(s): Goh, T., Joi, S., Himura, T., Fukaki, H.
  • Journal Title: Development Volume: 139 Issue: 5 Pages: 883-893
  • DOI: 10.1242/dev.071928
  • Peer Reviewed
• [Journal Article] Analyzing lateral root development : hoW to move forward. (2012)
  • Author(s): De Smet, I., White, P., Bengough, A. G., Dupuy, L., Parizot, B., tasimiro, I. He ids tra, R., Laskowski, M., Lepetit, M., Hochholdinger, F., Draye, X., Broadley, M., Peret, B, Zhang, H., Hammond, J. P., Fukaki, H., Mooney, S., Lynch, J. P., Nacry, P., Schurr, U., Bennett, M., Laplaze, L., Ben fey, P and Beecknan, T.
  • Journal Title: Plant Cell Volume: 24 Issue: 1 Pages: 15-20
  • DOI: 10.1105/tpc.111.094292
  • Peer Reviewed
• [Journal Article] RLF, a cytochrome b_5 heme/steroid binding protein, controls lateral root formation independently of ARF7/19-mediated auxin signaling in Arabidopsis thaliana (2010)
  • Author(s): Ikeyama Y., Tasaka M., and Fukaki H.
  • Journal Title: Plant J. Volume: 62 Pages: 862-875
  • Peer Reviewed
• [Journal Article] Auxin control of root development. Cold Spring Harb (2010)
  • Author(s): Overvoorde P., Fukaki H., and Beeckman T.
  • Journal Title: Perspect. Biol. Volume: 2(6)
  • Peer Reviewed
• [Journal Article] RLF, a cytochrome b_5 heme/steroid binding protein, controls lateral root formation independently of ARF7/19-mediated auxin signaling in Arabidopsis thaliana. (2010)
  • Author(s): Ikeyama, Y., Tasaka, M., Fukaki, H.
  • Journal Title: The Plant Journal Volume: 62 Pages: 862-875
  • Peer Reviewed
• [Journal Article] Auxin control of root development. (2010)
  • Author(s): Overvoorde, P., Fukaki, H., Beeckman, T.
  • Journal Title: Cold Spring Harb.Perspect.Biol. Volume: 2
  • Peer Reviewed
• [Presentation] 根系の成長システム解明に向けて-遺伝子制御ネットワーク研究から見えること- (2012)
  • Author(s): 深城英弘
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 姫路
• [Presentation] The role of SHY2/IAA3-mediated auxin signaling in Arabidopsis lateral root formation (2012)
  • Author(s): Tatsuaki Goh, Hiroyuki Kasahara, Tetsuro Mimura, Yuji Kamiya, Hidehiro Fukaki
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
• [Presentation] 根系の成長システム解明に向けてー遺伝子制御ネットワーク研究から見えることー (2012)
  • Author(s): 深城英弘
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 兵庫県立大学（姫路）
  • Invited
• [Presentation] The establishment of asymmetry in Arabidopsis lateral root founder cells is regulated by auxin-inducible LBD/ASL proteins (2011)
  • Author(s): Hidehiro Fukaki
  • Organizer: The 3^<rd> Korea-Japan Young Plant Scientist Symposium
  • Place of Presentation: POSTECH,Korea
• [Presentation] シロイヌナズナ根端メリステムの形成・維持の制御機構 (2011)
  • Author(s): 深城英弘
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京
• [Presentation] 側根形成開始における細胞分裂の非対称性の制御 (2011)
  • Author(s): 郷達明,城井駿平,三村徹郎,深城英弘
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京
• [Presentation] オーキシン誘導性LBD/ASLメンミーによる側根の形成開始の制御機構 (2011)
  • Author(s): 郷達明,三村徹郎,深城英弘
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] シロイヌナズナ側根形成における時空問特異的なオーキシン応答の制御 (2011)
  • Author(s): 城井駿平,小野田誠,郷達明,三村徹郎,田坂昌生,深城英弘
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] シロイヌナズナ側根欠失変異体slrのサプレッサー変異体ssl1/hastyの解析 (2011)
  • Author(s): 徳永綾子,郷達明,三村徹郎,田坂昌生,深城英弘
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] シロイヌナズナ側根形成におけるLBD16/ASL18の下流遺伝子LLPL2の解析 (2011)
  • Author(s): 横山碧,上原健生,郷達明,奥島葉子,三村徹郎,田坂昌生,深城英弘
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] Auxin and lateral root development : Transcriptional cascades regulating lateral root initiation in Arabidopsis thaliana (2010)
  • Author(s): Hidehiro Fukaki
  • Organizer: 第22回韓国分子細胞生物学会年会
  • Place of Presentation: ソウル(韓国)(招待講演)
  • Year and Date: 2010-10-07
• [Presentation] Auxin-inducible LBD/ASL proteins regulate Arabidopsis lateral root initiation through activating the first asymmetric cell divisions of protoxylem pericycle (2010)
  • Author(s): Tatsuaki Goh, Tetsuro Mimura, and Hidehiro Fukaki
  • Organizer: 第32回日本分子生物学会年会、第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸
• [Presentation] Auxin and Lateral Root Development : Transcriptional Cascades Regulating Lateral Root Initiation in Arabidopsis thaliana (2010)
  • Author(s): Hidehiiro Fukaki
  • Organizer: Korean Society of Molecular and Cell Biology, Symposium
  • Place of Presentation: Seoul, KOREA
• [Presentation] シロイヌナズナ側根形成における時期特異的・空問特異的なオーキシン応答の制御 (2010)
  • Author(s): 城井駿平,小野田誠,郷達明,三村徹郎,田坂昌生,深城英弘
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 春日井
• [Presentation] シロイヌナズナ側根欠失変異体slrのサプレッサー変異体ssl1/hastyの解析 (2010)
  • Author(s): 徳永綾子,郷達明,三村徹郎,田坂昌生,深城英弘
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 春日井
• [Presentation] シロイヌナズナ側根形成におけるLBD16/ASL18の下流遺伝子LLPL2の解析 (2010)
  • Author(s): 横山碧,上原健生,郷達明,奥島葉子,三村徹郎,田坂昌生,深城英弘
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 春日井
• [Presentation] Auxin-inducible LBD/ASL members regulate lateral root formation (2010)
  • Author(s): Tatsuaki Goh, Tetsuro Mimura, and Hidehiro Fukaki
  • Organizer: The 21^<st> conference on Arabidopsis research
  • Place of Presentation: Yokohama, Japan
• [Book] オーキシンを介した側根形成の分子機構,植物の生長調節Vol. 47 (2012)
  • Author(s): 深城英弘,郷達明
  • Total Pages: 76
  • Publisher: 植物化学調節学会
• [Book] 維管束植物の根系構築と側根発生の機構「細胞履歴に基づく植物の形態形成」高等研報告書1004 (2011)
  • Author(s): 城英弘
  • Total Pages: 158
  • Publisher: 財団法人国際高等研究所
• [Book] オーキシン応答における遺伝子発現制御「植物のシグナル伝達-分子と応答-」 (2010)
  • Author(s): 深城英弘
  • Total Pages: 240
  • Publisher: 共立出版
• [Remarks] 所属機関研究者紹介ホームページ
  • URL: http://www.edu.kobe-u.ac.jp/fsci-biol/staff/h-fukaki.html
• [Remarks] 研究室ホームページ
  • URL: http://www.research.kobe-u.ac.jp/fsci-fukaki/fukaki/top.html
• [Remarks]
  • URL: http://www.edu.kobe-u.ac.jp/fsci-biol/staff/h-fukaki.html
• [Remarks]
  • URL: http://www.research.kobe-u.ac.jp/fsci-fukaki/fukaki/fukaki_laboratory.html